Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcriptional factor SWI5

Gene

SWI5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri550 – 57425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri580 – 60425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri609 – 63224C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
DNA bindingi647 – 65913A.T hookAdd
BLAST

GO - Molecular functioni

  • mediator complex binding Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II regulatory region DNA binding Source: SGD
  • RNA polymerase II transcription factor activity, sequence-specific transcription regulatory region DNA binding Source: SGD

GO - Biological processi

  • positive regulation of mating type switching Source: SGD
  • positive regulation of transcription involved in exit from mitosis, from RNA polymerase II promoter Source: SGD
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29743-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional factor SWI5
Gene namesi
Name:SWI5
Ordered Locus Names:YDR146C
ORF Names:YD8358.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR146C.
SGDiS000002553. SWI5.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi522 – 5221S → A: Constitutive nuclear entry; when associated with A-646 and A-664. 1 Publication
Mutagenesisi646 – 6461S → A: Constitutive nuclear entry; when associated with A-522 and A-664. 1 Publication
Mutagenesisi664 – 6641S → A: Constitutive nuclear entry; when associated with A-522 and A-646. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Transcriptional factor SWI5PRO_0000046855Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251PhosphoserineCombined sources
Modified residuei278 – 2781PhosphoserineCombined sources
Modified residuei300 – 3001PhosphoserineCombined sources
Modified residuei339 – 3391PhosphothreonineCombined sources
Modified residuei376 – 3761PhosphoserineCombined sources
Modified residuei488 – 4881PhosphoserineCombined sources
Modified residuei492 – 4921PhosphoserineCombined sources
Modified residuei505 – 5051PhosphoserineCombined sources
Modified residuei522 – 5221Phosphoserine; by CDC28Combined sources1 Publication
Modified residuei646 – 6461Phosphoserine; by CDC28Combined sources1 Publication
Modified residuei664 – 6641Phosphoserine; by CDC281 Publication

Post-translational modificationi

Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur.
Phosphorylated by PHO85.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08153.

PTM databases

iPTMnetiP08153.

Interactioni

GO - Molecular functioni

  • mediator complex binding Source: SGD

Protein-protein interaction databases

BioGridi32200. 226 interactions.
DIPiDIP-1461N.
IntActiP08153. 9 interactions.
MINTiMINT-399696.

Structurei

Secondary structure

1
709
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi538 – 5403Combined sources
Beta strandi543 – 5453Combined sources
Turni546 – 5483Combined sources
Beta strandi549 – 5513Combined sources
Beta strandi563 – 5664Combined sources
Helixi567 – 5748Combined sources
Turni575 – 5773Combined sources
Beta strandi591 – 5944Combined sources
Helixi595 – 6017Combined sources
Helixi602 – 6043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NCSNMR-A532-578[»]
1ZFDNMR-A577-608[»]
ProteinModelPortaliP08153.
SMRiP08153. Positions 532-627, 631-707.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08153.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi635 – 65925Nuclear localization signalSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 A.T hook DNA-binding domain.Curated
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri550 – 57425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri580 – 60425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri609 – 63224C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00790000123023.
HOGENOMiHOG000001123.
InParanoidiP08153.
KOiK09202.
OrthoDBiEOG092C2Q0K.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD
60 70 80 90 100
LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ
110 120 130 140 150
SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS
160 170 180 190 200
MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE
210 220 230 240 250
LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS
260 270 280 290 300
PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS
310 320 330 340 350
PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN
360 370 380 390 400
VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN
410 420 430 440 450
TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA
460 470 480 490 500
RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK
510 520 530 540 550
YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF
560 570 580 590 600
ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH
610 620 630 640 650
KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR
660 670 680 690 700
GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM

VSPMKTNQR
Length:709
Mass (Da):79,775
Last modified:August 1, 1988 - v1
Checksum:iBEF5ED5BFB6E30F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti514 – 5141E → K in AAU09695 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.
AY723778 Genomic DNA. Translation: AAU09695.1.
BK006938 Genomic DNA. Translation: DAA11989.1.
PIRiS00342. TWBYS5.
RefSeqiNP_010430.1. NM_001180453.1.

Genome annotation databases

EnsemblFungiiYDR146C; YDR146C; YDR146C.
GeneIDi851724.
KEGGisce:YDR146C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.
AY723778 Genomic DNA. Translation: AAU09695.1.
BK006938 Genomic DNA. Translation: DAA11989.1.
PIRiS00342. TWBYS5.
RefSeqiNP_010430.1. NM_001180453.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NCSNMR-A532-578[»]
1ZFDNMR-A577-608[»]
ProteinModelPortaliP08153.
SMRiP08153. Positions 532-627, 631-707.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32200. 226 interactions.
DIPiDIP-1461N.
IntActiP08153. 9 interactions.
MINTiMINT-399696.

PTM databases

iPTMnetiP08153.

Proteomic databases

MaxQBiP08153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR146C; YDR146C; YDR146C.
GeneIDi851724.
KEGGisce:YDR146C.

Organism-specific databases

EuPathDBiFungiDB:YDR146C.
SGDiS000002553. SWI5.

Phylogenomic databases

GeneTreeiENSGT00790000123023.
HOGENOMiHOG000001123.
InParanoidiP08153.
KOiK09202.
OrthoDBiEOG092C2Q0K.

Enzyme and pathway databases

BioCyciYEAST:G3O-29743-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08153.
PROiP08153.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSWI5_YEAST
AccessioniPrimary (citable) accession number: P08153
Secondary accession number(s): D6VSC9, E9P945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: September 7, 2016
This is version 187 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.