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P08153

- SWI5_YEAST

UniProt

P08153 - SWI5_YEAST

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Protein

Transcriptional factor SWI5

Gene
SWI5, YDR146C, YD8358.03C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri550 – 57425C2H2-type 1Add
BLAST
Zinc fingeri580 – 60425C2H2-type 2Add
BLAST
Zinc fingeri609 – 63224C2H2-type 3Add
BLAST
DNA bindingi647 – 65913A.T hook1 PublicationAdd
BLAST

GO - Molecular functioni

  1. mediator complex binding Source: SGD
  2. metal ion binding Source: UniProtKB-KW
  3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: SGD
  4. sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD

GO - Biological processi

  1. positive regulation of mating type switching Source: SGD
  2. positive regulation of transcription on exit from mitosis, from RNA polymerase II promoter Source: SGD
  3. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29743-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional factor SWI5
Gene namesi
Name:SWI5
Ordered Locus Names:YDR146C
ORF Names:YD8358.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR146c.
SGDiS000002553. SWI5.

Subcellular locationi

Nucleus. Cytoplasm
Note: Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi522 – 5221S → A: Constitutive nuclear entry; when associated with A-646 and A-664.
Mutagenesisi646 – 6461S → A: Constitutive nuclear entry; when associated with A-522 and A-664.
Mutagenesisi664 – 6641S → A: Constitutive nuclear entry; when associated with A-522 and A-646.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Transcriptional factor SWI5PRO_0000046855Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251Phosphoserine1 Publication
Modified residuei278 – 2781Phosphoserine1 Publication
Modified residuei300 – 3001Phosphoserine1 Publication
Modified residuei339 – 3391Phosphothreonine1 Publication
Modified residuei376 – 3761Phosphoserine1 Publication
Modified residuei488 – 4881Phosphoserine1 Publication
Modified residuei492 – 4921Phosphoserine2 Publications
Modified residuei505 – 5051Phosphoserine2 Publications
Modified residuei522 – 5221Phosphoserine; by CDC282 Publications
Modified residuei646 – 6461Phosphoserine; by CDC282 Publications
Modified residuei664 – 6641Phosphoserine; by CDC281 Publication

Post-translational modificationi

Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur.2 Publications
Phosphorylated by PHO85.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08153.
PaxDbiP08153.

Expressioni

Gene expression databases

GenevestigatoriP08153.

Interactioni

Protein-protein interaction databases

BioGridi32200. 224 interactions.
DIPiDIP-1461N.
IntActiP08153. 9 interactions.
MINTiMINT-399696.
STRINGi4932.YDR146C.

Structurei

Secondary structure

1
709
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi538 – 5403
Beta strandi543 – 5453
Turni546 – 5483
Beta strandi549 – 5513
Beta strandi563 – 5664
Helixi567 – 5748
Turni575 – 5773
Beta strandi591 – 5944
Helixi595 – 6017
Helixi602 – 6043

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NCSNMR-A532-578[»]
1ZFDNMR-A577-608[»]
ProteinModelPortaliP08153.
SMRiP08153. Positions 532-707.

Miscellaneous databases

EvolutionaryTraceiP08153.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi635 – 65925Nuclear localization signal Reviewed predictionAdd
BLAST

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri550 – 57425C2H2-type 1Add
BLAST
Zinc fingeri580 – 60425C2H2-type 2Add
BLAST
Zinc fingeri609 – 63224C2H2-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00620000087922.
HOGENOMiHOG000001123.
KOiK09202.
OrthoDBiEOG7Q2NDV.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR017956. AT_hook_DNA-bd_motif.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00384. AT_hook. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08153-1 [UniParc]FASTAAdd to Basket

« Hide

MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD    50
LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ 100
SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS 150
MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE 200
LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS 250
PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS 300
PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN 350
VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN 400
TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA 450
RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK 500
YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF 550
ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH 600
KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR 650
GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM 700
VSPMKTNQR 709
Length:709
Mass (Da):79,775
Last modified:August 1, 1988 - v1
Checksum:iBEF5ED5BFB6E30F6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti514 – 5141E → K in AAU09695. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.
AY723778 Genomic DNA. Translation: AAU09695.1.
BK006938 Genomic DNA. Translation: DAA11989.1.
PIRiS00342. TWBYS5.
RefSeqiNP_010430.1. NM_001180453.1.

Genome annotation databases

EnsemblFungiiYDR146C; YDR146C; YDR146C.
GeneIDi851724.
KEGGisce:YDR146C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06978 Genomic DNA. Translation: CAA30040.1 .
Z50046 Genomic DNA. Translation: CAA90369.1 .
AY723778 Genomic DNA. Translation: AAU09695.1 .
BK006938 Genomic DNA. Translation: DAA11989.1 .
PIRi S00342. TWBYS5.
RefSeqi NP_010430.1. NM_001180453.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NCS NMR - A 532-578 [» ]
1ZFD NMR - A 577-608 [» ]
ProteinModelPortali P08153.
SMRi P08153. Positions 532-707.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32200. 224 interactions.
DIPi DIP-1461N.
IntActi P08153. 9 interactions.
MINTi MINT-399696.
STRINGi 4932.YDR146C.

Proteomic databases

MaxQBi P08153.
PaxDbi P08153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR146C ; YDR146C ; YDR146C .
GeneIDi 851724.
KEGGi sce:YDR146C.

Organism-specific databases

CYGDi YDR146c.
SGDi S000002553. SWI5.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00620000087922.
HOGENOMi HOG000001123.
KOi K09202.
OrthoDBi EOG7Q2NDV.

Enzyme and pathway databases

BioCyci YEAST:G3O-29743-MONOMER.

Miscellaneous databases

EvolutionaryTracei P08153.
NextBioi 969436.

Gene expression databases

Genevestigatori P08153.

Family and domain databases

Gene3Di 3.30.160.60. 2 hits.
InterProi IPR017956. AT_hook_DNA-bd_motif.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 2 hits.
[Graphical view ]
SMARTi SM00384. AT_hook. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene."
    Stillman D.J., Bankier A.T., Seddon A., Groenhout E.G., Nasmyth K.A.
    EMBO J. 7:485-494(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA."
    Nagai K., Nakaseko Y., Nasmyth K.A., Rhodes D.
    Nature 332:284-286(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
  6. "The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5."
    Moll T., Tebb G., Surana U., Robitsch H., Nasmyth K.
    Cell 66:743-758(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-522; SER-646 AND SER-664 BY CDC28, MUTAGENESIS, SUBCELLULAR LOCATION.
  7. "Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast."
    Measday V., McBride H., Moffat J., Stillman D., Andrews B.J.
    Mol. Microbiol. 35:825-834(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PHO85.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-646, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492 AND SER-505, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-278; SER-300; THR-339; SER-376; SER-488; SER-492 AND SER-522, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet."
    Neuhaus D., Nakaseko Y., Schwabe J.W.R., Klug A.
    J. Mol. Biol. 228:637-651(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 540-608.

Entry informationi

Entry nameiSWI5_YEAST
AccessioniPrimary (citable) accession number: P08153
Secondary accession number(s): D6VSC9, E9P945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 11, 2014
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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