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P08153 (SWI5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 166. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional factor SWI5
Gene names
Name:SWI5
Ordered Locus Names:YDR146C
ORF Names:YD8358.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length709 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.

Subcellular location

Nucleus. Cytoplasm. Note: Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases. Ref.6

Post-translational modification

Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur. Ref.6 Ref.7

Phosphorylated by PHO85. Ref.6 Ref.7

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 A.T hook DNA-binding domain.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of mating type switching

Inferred from mutant phenotype PubMed 6436497. Source: SGD

positive regulation of transcription on exit from mitosis, from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 9017392PubMed 9529390. Source: SGD

regulation of transcription involved in G1/S transition of mitotic cell cycle

Inferred from mutant phenotype PubMed 10409653. Source: SGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 8816483. Source: SGD

nucleus

Inferred from direct assay PubMed 8816483. Source: SGD

   Molecular_functionRNA polymerase II distal enhancer sequence-specific DNA binding

Inferred from direct assay Ref.1. Source: SGD

mediator complex binding

Inferred from mutant phenotype PubMed 11562354. Source: SGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity

Inferred from mutant phenotype PubMed 10319811PubMed 11562354. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 709709Transcriptional factor SWI5
PRO_0000046855

Regions

Zinc finger550 – 57425C2H2-type 1
Zinc finger580 – 60425C2H2-type 2
Zinc finger609 – 63224C2H2-type 3
DNA binding647 – 65913A.T hook Ref.5
Motif635 – 65925Nuclear localization signal Potential

Amino acid modifications

Modified residue2251Phosphoserine Ref.11
Modified residue2781Phosphoserine Ref.11
Modified residue3001Phosphoserine Ref.11
Modified residue3391Phosphothreonine Ref.11
Modified residue3761Phosphoserine Ref.11
Modified residue4881Phosphoserine Ref.11
Modified residue4921Phosphoserine Ref.10 Ref.11
Modified residue5051Phosphoserine Ref.9 Ref.10
Modified residue5221Phosphoserine; by CDC28 Ref.6 Ref.11
Modified residue6461Phosphoserine; by CDC28 Ref.6 Ref.9
Modified residue6641Phosphoserine; by CDC28 Ref.6

Experimental info

Mutagenesis5221S → A: Constitutive nuclear entry; when associated with A-646 and A-664.
Mutagenesis6461S → A: Constitutive nuclear entry; when associated with A-522 and A-664.
Mutagenesis6641S → A: Constitutive nuclear entry; when associated with A-522 and A-646.
Sequence conflict5141E → K in AAU09695. Ref.4

Secondary structure

............... 709
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08153 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: BEF5ED5BFB6E30F6

FASTA70979,775
        10         20         30         40         50         60 
MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD LGNILNLNYG 

        70         80         90        100        110        120 
ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ SMNFDLENNS REVTLNATSL 

       130        140        150        160        170        180 
LNENRLNQDS GMTVYQKTMS DKPHDEKKIS MADNLLSTIN KSEINKGFDR NLGELLLQQQ 

       190        200        210        220        230        240 
QELREQLRAQ QEANKKLELE LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV 

       250        260        270        280        290        300 
YANSLSPMIS PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS 

       310        320        330        340        350        360 
PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN VEDQESEYSE 

       370        380        390        400        410        420 
KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN TPSVNKVSLS SSYIDQYTPR 

       430        440        450        460        470        480 
GKQLHFSSIS ENALGINAAT PHLKPPSQQA RHREGVFNDL DPNVLTKNTD NEGDDNEENE 

       490        500        510        520        530        540 
PESRFVISET PSPVLKSQSK YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK 

       550        560        570        580        590        600 
YVKEMPDKTF ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH 

       610        620        630        640        650        660 
KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR GRPRKDGTSS 

       670        680        690        700 
VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM VSPMKTNQR 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene."
Stillman D.J., Bankier A.T., Seddon A., Groenhout E.G., Nasmyth K.A.
EMBO J. 7:485-494(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA."
Nagai K., Nakaseko Y., Nasmyth K.A., Rhodes D.
Nature 332:284-286(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING.
[6]"The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5."
Moll T., Tebb G., Surana U., Robitsch H., Nasmyth K.
Cell 66:743-758(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-522; SER-646 AND SER-664 BY CDC28, MUTAGENESIS, SUBCELLULAR LOCATION.
[7]"Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast."
Measday V., McBride H., Moffat J., Stillman D., Andrews B.J.
Mol. Microbiol. 35:825-834(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY PHO85.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-646, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492 AND SER-505, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-278; SER-300; THR-339; SER-376; SER-488; SER-492 AND SER-522, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet."
Neuhaus D., Nakaseko Y., Schwabe J.W.R., Klug A.
J. Mol. Biol. 228:637-651(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 540-608.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.
AY723778 Genomic DNA. Translation: AAU09695.1.
BK006938 Genomic DNA. Translation: DAA11989.1.
PIRTWBYS5. S00342.
RefSeqNP_010430.1. NM_001180453.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NCSNMR-A532-578[»]
1ZFDNMR-A577-608[»]
ProteinModelPortalP08153.
SMRP08153. Positions 532-695.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32200. 224 interactions.
DIPDIP-1461N.
IntActP08153. 9 interactions.
MINTMINT-399696.
STRING4932.YDR146C.

Proteomic databases

PaxDbP08153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR146C; YDR146C; YDR146C.
GeneID851724.
KEGGsce:YDR146C.

Organism-specific databases

CYGDYDR146c.
SGDS000002553. SWI5.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00620000087922.
HOGENOMHOG000001123.
KOK09202.
OrthoDBEOG7Q2NDV.

Enzyme and pathway databases

BioCycYEAST:G3O-29743-MONOMER.

Gene expression databases

GenevestigatorP08153.

Family and domain databases

Gene3D3.30.160.60. 2 hits.
InterProIPR017956. AT_hook_DNA-bd_motif.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTSM00384. AT_hook. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP08153.
NextBio969436.

Entry information

Entry nameSWI5_YEAST
AccessionPrimary (citable) accession number: P08153
Secondary accession number(s): D6VSC9, E9P945
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 19, 2014
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references