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P08153

- SWI5_YEAST

UniProt

P08153 - SWI5_YEAST

Protein

Transcriptional factor SWI5

Gene

SWI5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 169 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri550 – 57425C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri580 – 60425C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri609 – 63224C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi647 – 65913A.T hookAdd
    BLAST

    GO - Molecular functioni

    1. mediator complex binding Source: SGD
    2. metal ion binding Source: UniProtKB-KW
    3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: SGD
    4. sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD

    GO - Biological processi

    1. positive regulation of mating type switching Source: SGD
    2. positive regulation of transcription on exit from mitosis, from RNA polymerase II promoter Source: SGD
    3. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29743-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional factor SWI5
    Gene namesi
    Name:SWI5
    Ordered Locus Names:YDR146C
    ORF Names:YD8358.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR146c.
    SGDiS000002553. SWI5.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication
    Note: Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases.

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi522 – 5221S → A: Constitutive nuclear entry; when associated with A-646 and A-664. 1 Publication
    Mutagenesisi646 – 6461S → A: Constitutive nuclear entry; when associated with A-522 and A-664. 1 Publication
    Mutagenesisi664 – 6641S → A: Constitutive nuclear entry; when associated with A-522 and A-646. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 709709Transcriptional factor SWI5PRO_0000046855Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei225 – 2251Phosphoserine2 Publications
    Modified residuei278 – 2781Phosphoserine2 Publications
    Modified residuei300 – 3001Phosphoserine2 Publications
    Modified residuei339 – 3391Phosphothreonine2 Publications
    Modified residuei376 – 3761Phosphoserine2 Publications
    Modified residuei488 – 4881Phosphoserine2 Publications
    Modified residuei492 – 4921Phosphoserine3 Publications
    Modified residuei505 – 5051Phosphoserine3 Publications
    Modified residuei522 – 5221Phosphoserine; by CDC283 Publications
    Modified residuei646 – 6461Phosphoserine; by CDC283 Publications
    Modified residuei664 – 6641Phosphoserine; by CDC282 Publications

    Post-translational modificationi

    Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur.
    Phosphorylated by PHO85.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP08153.
    PaxDbiP08153.

    Expressioni

    Gene expression databases

    GenevestigatoriP08153.

    Interactioni

    Protein-protein interaction databases

    BioGridi32200. 224 interactions.
    DIPiDIP-1461N.
    IntActiP08153. 9 interactions.
    MINTiMINT-399696.
    STRINGi4932.YDR146C.

    Structurei

    Secondary structure

    1
    709
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi538 – 5403
    Beta strandi543 – 5453
    Turni546 – 5483
    Beta strandi549 – 5513
    Beta strandi563 – 5664
    Helixi567 – 5748
    Turni575 – 5773
    Beta strandi591 – 5944
    Helixi595 – 6017
    Helixi602 – 6043

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NCSNMR-A532-578[»]
    1ZFDNMR-A577-608[»]
    ProteinModelPortaliP08153.
    SMRiP08153. Positions 532-707.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08153.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi635 – 65925Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 A.T hook DNA-binding domain.Curated
    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri550 – 57425C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri580 – 60425C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri609 – 63224C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00620000087922.
    HOGENOMiHOG000001123.
    KOiK09202.
    OrthoDBiEOG7Q2NDV.

    Family and domain databases

    Gene3Di3.30.160.60. 2 hits.
    InterProiIPR017956. AT_hook_DNA-bd_motif.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 2 hits.
    [Graphical view]
    SMARTiSM00384. AT_hook. 1 hit.
    SM00355. ZnF_C2H2. 2 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08153-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD    50
    LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ 100
    SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS 150
    MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE 200
    LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS 250
    PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS 300
    PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN 350
    VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN 400
    TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA 450
    RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK 500
    YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF 550
    ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH 600
    KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR 650
    GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM 700
    VSPMKTNQR 709
    Length:709
    Mass (Da):79,775
    Last modified:August 1, 1988 - v1
    Checksum:iBEF5ED5BFB6E30F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti514 – 5141E → K in AAU09695. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06978 Genomic DNA. Translation: CAA30040.1.
    Z50046 Genomic DNA. Translation: CAA90369.1.
    AY723778 Genomic DNA. Translation: AAU09695.1.
    BK006938 Genomic DNA. Translation: DAA11989.1.
    PIRiS00342. TWBYS5.
    RefSeqiNP_010430.1. NM_001180453.1.

    Genome annotation databases

    EnsemblFungiiYDR146C; YDR146C; YDR146C.
    GeneIDi851724.
    KEGGisce:YDR146C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06978 Genomic DNA. Translation: CAA30040.1 .
    Z50046 Genomic DNA. Translation: CAA90369.1 .
    AY723778 Genomic DNA. Translation: AAU09695.1 .
    BK006938 Genomic DNA. Translation: DAA11989.1 .
    PIRi S00342. TWBYS5.
    RefSeqi NP_010430.1. NM_001180453.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NCS NMR - A 532-578 [» ]
    1ZFD NMR - A 577-608 [» ]
    ProteinModelPortali P08153.
    SMRi P08153. Positions 532-707.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32200. 224 interactions.
    DIPi DIP-1461N.
    IntActi P08153. 9 interactions.
    MINTi MINT-399696.
    STRINGi 4932.YDR146C.

    Proteomic databases

    MaxQBi P08153.
    PaxDbi P08153.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR146C ; YDR146C ; YDR146C .
    GeneIDi 851724.
    KEGGi sce:YDR146C.

    Organism-specific databases

    CYGDi YDR146c.
    SGDi S000002553. SWI5.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00620000087922.
    HOGENOMi HOG000001123.
    KOi K09202.
    OrthoDBi EOG7Q2NDV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29743-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P08153.
    NextBioi 969436.

    Gene expression databases

    Genevestigatori P08153.

    Family and domain databases

    Gene3Di 3.30.160.60. 2 hits.
    InterProi IPR017956. AT_hook_DNA-bd_motif.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 2 hits.
    [Graphical view ]
    SMARTi SM00384. AT_hook. 1 hit.
    SM00355. ZnF_C2H2. 2 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene."
      Stillman D.J., Bankier A.T., Seddon A., Groenhout E.G., Nasmyth K.A.
      EMBO J. 7:485-494(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA."
      Nagai K., Nakaseko Y., Nasmyth K.A., Rhodes D.
      Nature 332:284-286(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
    6. "The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5."
      Moll T., Tebb G., Surana U., Robitsch H., Nasmyth K.
      Cell 66:743-758(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-522; SER-646 AND SER-664 BY CDC28, MUTAGENESIS, SUBCELLULAR LOCATION.
    7. "Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast."
      Measday V., McBride H., Moffat J., Stillman D., Andrews B.J.
      Mol. Microbiol. 35:825-834(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY PHO85.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-646, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492 AND SER-505, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-278; SER-300; THR-339; SER-376; SER-488; SER-492 AND SER-522, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet."
      Neuhaus D., Nakaseko Y., Schwabe J.W.R., Klug A.
      J. Mol. Biol. 228:637-651(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 540-608.

    Entry informationi

    Entry nameiSWI5_YEAST
    AccessioniPrimary (citable) accession number: P08153
    Secondary accession number(s): D6VSC9, E9P945
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 169 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 688 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3