Reviewed, UniProtKB/Swiss-Prot P08153 (SWI5_YEAST)

Names and origin

Protein names

Recommended name:
    Transcriptional factor SWI5

Gene names

Name:	SWI5
Ordered Locus Names:	YDR146C
ORF Names:	YD8358.03C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.
Subcellular location	Nucleus. Cytoplasm. Note: Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases. Ref.4
Post-translational modification	Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur. Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.10 Phosphorylated by PHO85. Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.10
Miscellaneous	Present with 688 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 A.T hook DNA-binding domain. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription involved in G1 phase of mitotic cell cycle Inferred from direct assay. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD transcription activator activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	1	EBI-18633,EBI-4192
MSN5	P52918	2	EBI-18633,EBI-11420

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 709

709

Transcriptional factor SWI5

PRO_0000046855

Regions

Zinc finger

550 – 574

25

C2H2-type 1

Zinc finger

580 – 604

25

C2H2-type 2

Zinc finger

609 – 632

24

C2H2-type 3

DNA binding

647 – 659

13

A.T hook Ref.3

Motif

635 – 659

25

Nuclear localization signal Potential

Amino acid modifications

Modified residue

278

1

Phosphoserine Ref.9

Modified residue

492

1

Phosphoserine Ref.7 Ref.10

Modified residue

505

1

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

522

1

Phosphoserine; by CDC28

Modified residue

646

1

Phosphoserine; by CDC28 Ref.8

Modified residue

664

1

Phosphoserine; by CDC28

Experimental info

Mutagenesis

522

1

S → A: Constitutive nuclear entry; when associated with A-646 and A-664.

Mutagenesis

646

1

S → A: Constitutive nuclear entry; when associated with A-522 and A-664.

Mutagenesis

664

1

S → A: Constitutive nuclear entry; when associated with A-522 and A-646.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

709

Helix Strand Turn

Details...

Helix

538 – 540

3

Beta strand

543 – 545

3

Turn

546 – 548

3

Beta strand

549 – 551

3

Beta strand

563 – 566

4

Helix

567 – 574

8

Turn

575 – 577

3

Beta strand

591 – 594

4

Helix

595 – 601

7

Helix

602 – 604

3

Sequences

	Sequence		Length	Mass (Da)
	P08153-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: BEF5ED5BFB6E30F6	FASTA	709	79,775
	10 20 30 40 50 60 MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD LGNILNLNYG 70 80 90 100 110 120 ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ SMNFDLENNS REVTLNATSL 130 140 150 160 170 180 LNENRLNQDS GMTVYQKTMS DKPHDEKKIS MADNLLSTIN KSEINKGFDR NLGELLLQQQ 190 200 210 220 230 240 QELREQLRAQ QEANKKLELE LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV 250 260 270 280 290 300 YANSLSPMIS PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS 310 320 330 340 350 360 PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN VEDQESEYSE 370 380 390 400 410 420 KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN TPSVNKVSLS SSYIDQYTPR 430 440 450 460 470 480 GKQLHFSSIS ENALGINAAT PHLKPPSQQA RHREGVFNDL DPNVLTKNTD NEGDDNEENE 490 500 510 520 530 540 PESRFVISET PSPVLKSQSK YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK 550 560 570 580 590 600 YVKEMPDKTF ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH 610 620 630 640 650 660 KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR GRPRKDGTSS 670 680 690 700 VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM VSPMKTNQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene." Stillman D.J., Bankier A.T., Seddon A., Groenhout E.G., Nasmyth K.A. EMBO J. 7:485-494(1988) [PubMed: 3284746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[3]	"Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA." Nagai K., Nakaseko Y., Nasmyth K.A., Rhodes D. Nature 332:284-286(1988) [PubMed: 2831463] [Abstract] Cited for: DNA-BINDING.
[4]	"The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5." Moll T., Tebb G., Surana U., Robitsch H., Nasmyth K. Cell 66:743-758(1991) [PubMed: 1652372] [Abstract] Cited for: PHOSPHORYLATION, MUTAGENESIS, SUBCELLULAR LOCATION.
[5]	"Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast." Measday V., McBride H., Moffat J., Stillman D., Andrews B.J. Mol. Microbiol. 35:825-834(2000) [PubMed: 10692159] [Abstract] Cited for: PHOSPHORYLATION BY PHO85.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492, MASS SPECTROMETRY.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505 AND SER-646, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278 AND SER-505, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492 AND SER-505, MASS SPECTROMETRY.
[11]	"Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet." Neuhaus D., Nakaseko Y., Schwabe J.W.R., Klug A. J. Mol. Biol. 228:637-651(1992) [PubMed: 1453468] [Abstract] Cited for: STRUCTURE BY NMR OF 540-608.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.

PIR

TWBYS5. S00342.

RefSeq

NP_010430.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NCS	NMR	-	A	532-578	[»]
1ZFD	NMR	-	A	577-608	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1461N.

IntAct

P08153. 18 interactions.

STRING

P08153.

Proteomic databases

PRIDE

P08153.

Genome annotation databases

Ensembl

YDR146C; YDR146C; YDR146C; Saccharomyces cerevisiae. [Genome view]

GeneID

851724.

KEGG

sce:YDR146C.

NMPDR

fig|4932.3.peg.1179.

Organism-specific databases

CYGD

YDR146c.

SGD

S000002553. SWI5.

Phylogenomic databases

OrthoDB

EOG9BGBC2

Gene expression databases

ArrayExpress

P08153.

Genevestigator

P08153.

GermOnline

YDR146C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR017956. AT_hook_DNA-bd_motif.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.

Pfam

PF02178. AT_hook. 1 hit.
PF00096. zf-C2H2. 2 hits.
[Graphical view]

SMART

SM00384. AT_hook. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

969436.

Entry information

Entry name

SWI5_YEAST

Accession

Primary (citable) accession number: P08153

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	November 24, 2009
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)