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Protein

Transcriptional factor SWI5

Gene

SWI5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri550 – 574C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri580 – 604C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri609 – 632C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
DNA bindingi647 – 659A.T hookAdd BLAST13

GO - Molecular functioni

  • mediator complex binding Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II regulatory region DNA binding Source: SGD
  • RNA polymerase II transcription factor activity, sequence-specific transcription regulatory region DNA binding Source: SGD

GO - Biological processi

  • positive regulation of mating type switching Source: SGD
  • positive regulation of transcription involved in exit from mitosis, from RNA polymerase II promoter Source: SGD
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29743-MONOMER.
ReactomeiR-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional factor SWI5
Gene namesi
Name:SWI5
Ordered Locus Names:YDR146C
ORF Names:YD8358.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR146C.
SGDiS000002553. SWI5.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi522S → A: Constitutive nuclear entry; when associated with A-646 and A-664. 1 Publication1
Mutagenesisi646S → A: Constitutive nuclear entry; when associated with A-522 and A-664. 1 Publication1
Mutagenesisi664S → A: Constitutive nuclear entry; when associated with A-522 and A-646. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468551 – 709Transcriptional factor SWI5Add BLAST709

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei225PhosphoserineCombined sources1
Modified residuei278PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei339PhosphothreonineCombined sources1
Modified residuei376PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei492PhosphoserineCombined sources1
Modified residuei505PhosphoserineCombined sources1
Modified residuei522Phosphoserine; by CDC28Combined sources1 Publication1
Modified residuei646Phosphoserine; by CDC28Combined sources1 Publication1
Modified residuei664Phosphoserine; by CDC281 Publication1

Post-translational modificationi

Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur.
Phosphorylated by PHO85.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08153.
PRIDEiP08153.

PTM databases

iPTMnetiP08153.

Interactioni

GO - Molecular functioni

  • mediator complex binding Source: SGD

Protein-protein interaction databases

BioGridi32200. 226 interactors.
DIPiDIP-1461N.
IntActiP08153. 9 interactors.
MINTiMINT-399696.

Structurei

Secondary structure

1709
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi538 – 540Combined sources3
Beta strandi543 – 545Combined sources3
Turni546 – 548Combined sources3
Beta strandi549 – 551Combined sources3
Beta strandi563 – 566Combined sources4
Helixi567 – 574Combined sources8
Turni575 – 577Combined sources3
Beta strandi591 – 594Combined sources4
Helixi595 – 601Combined sources7
Helixi602 – 604Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NCSNMR-A532-578[»]
1ZFDNMR-A577-608[»]
ProteinModelPortaliP08153.
SMRiP08153.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08153.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi635 – 659Nuclear localization signalSequence analysisAdd BLAST25

Sequence similaritiesi

Contains 1 A.T hook DNA-binding domain.Curated
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri550 – 574C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri580 – 604C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri609 – 632C2H2-type 3PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00790000123023.
HOGENOMiHOG000001123.
InParanoidiP08153.
KOiK09202.
OrthoDBiEOG092C2Q0K.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD
60 70 80 90 100
LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ
110 120 130 140 150
SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS
160 170 180 190 200
MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE
210 220 230 240 250
LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS
260 270 280 290 300
PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS
310 320 330 340 350
PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN
360 370 380 390 400
VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN
410 420 430 440 450
TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA
460 470 480 490 500
RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK
510 520 530 540 550
YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF
560 570 580 590 600
ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH
610 620 630 640 650
KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR
660 670 680 690 700
GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM

VSPMKTNQR
Length:709
Mass (Da):79,775
Last modified:August 1, 1988 - v1
Checksum:iBEF5ED5BFB6E30F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti514E → K in AAU09695 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.
AY723778 Genomic DNA. Translation: AAU09695.1.
BK006938 Genomic DNA. Translation: DAA11989.1.
PIRiS00342. TWBYS5.
RefSeqiNP_010430.1. NM_001180453.1.

Genome annotation databases

EnsemblFungiiYDR146C; YDR146C; YDR146C.
GeneIDi851724.
KEGGisce:YDR146C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06978 Genomic DNA. Translation: CAA30040.1.
Z50046 Genomic DNA. Translation: CAA90369.1.
AY723778 Genomic DNA. Translation: AAU09695.1.
BK006938 Genomic DNA. Translation: DAA11989.1.
PIRiS00342. TWBYS5.
RefSeqiNP_010430.1. NM_001180453.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NCSNMR-A532-578[»]
1ZFDNMR-A577-608[»]
ProteinModelPortaliP08153.
SMRiP08153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32200. 226 interactors.
DIPiDIP-1461N.
IntActiP08153. 9 interactors.
MINTiMINT-399696.

PTM databases

iPTMnetiP08153.

Proteomic databases

MaxQBiP08153.
PRIDEiP08153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR146C; YDR146C; YDR146C.
GeneIDi851724.
KEGGisce:YDR146C.

Organism-specific databases

EuPathDBiFungiDB:YDR146C.
SGDiS000002553. SWI5.

Phylogenomic databases

GeneTreeiENSGT00790000123023.
HOGENOMiHOG000001123.
InParanoidiP08153.
KOiK09202.
OrthoDBiEOG092C2Q0K.

Enzyme and pathway databases

BioCyciYEAST:G3O-29743-MONOMER.
ReactomeiR-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

Miscellaneous databases

EvolutionaryTraceiP08153.
PROiP08153.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSWI5_YEAST
AccessioniPrimary (citable) accession number: P08153
Secondary accession number(s): D6VSC9, E9P945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 190 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.