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Protein

E3 SUMO-protein ligase EGR2

Gene

Egr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. Binds to the promoter region of ERBB2. May play a role in the regulation of hindbrain segmentation, might act in combination with the Hox network to specify odd and even rhombomeres, and might participate in the control of the expression of some of the homeobox containing genes.3 Publications
E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity.By similarity

Pathway: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri337 – 36125C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri367 – 38923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri395 – 41723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • brain segmentation Source: MGI
  • cellular response to cAMP Source: GO_Central
  • cellular response to gonadotropin stimulus Source: GO_Central
  • cellular response to organic substance Source: MGI
  • facial nerve structural organization Source: MGI
  • fat cell differentiation Source: BHF-UCL
  • learning or memory Source: Ensembl
  • motor neuron axon guidance Source: MGI
  • myelination Source: MGI
  • negative regulation of apoptotic process Source: GO_Central
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein export from nucleus Source: UniProtKB
  • protein sumoylation Source: UniProtKB-UniPathway
  • regulation of neuronal synaptic plasticity Source: GO_Central
  • regulation of ossification Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • response to insulin Source: Ensembl
  • rhombomere 3 development Source: MGI
  • rhombomere 3 formation Source: MGI
  • rhombomere 5 formation Source: MGI
  • rhythmic behavior Source: MGI
  • Schwann cell differentiation Source: MGI
  • skeletal muscle cell differentiation Source: MGI
  • transcription from RNA polymerase II promoter Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Activator, Ligase

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
REACT_309805. Transcriptional regulation of white adipocyte differentiation.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase EGR2 (EC:6.3.2.-)
Alternative name(s):
Early growth response protein 2
Short name:
EGR-2
Zinc finger protein Krox-20
Gene namesi
Name:Egr2
Synonyms:Egr-2, Krox-20, Zfp-25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95296. Egr2.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi174 – 1741Y → F: Abolishes interaction with WWP2; if associated with F-208. 1 Publication
Mutagenesisi208 – 2081Y → F: Abolishes interaction with WWP2; if associated with F-174. 1 Publication
Mutagenesisi377 – 3771S → A: Inhibits interaction with SFN. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470E3 SUMO-protein ligase EGR2PRO_0000047120Add
BLAST

Post-translational modificationi

Ubiquitinated by WWP2 leading to proteasomal degradation.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiP08152.

PTM databases

PhosphoSiteiP08152.

Expressioni

Tissue specificityi

Expressed in mammary tumors (at protein level). Expressed mainly in adult thymus and embryonic nervous system.1 Publication

Inductioni

Activated during G0/G1 transition in cultured cells.

Gene expression databases

BgeeiP08152.
CleanExiMM_EGR2.
ExpressionAtlasiP08152. baseline and differential.
GenevisibleiP08152. MM.

Interactioni

Subunit structurei

Interacts with HCFC1. Interacts with UBC9 (By similarity). Interacts with WWP2. Interacts with CITED1. Interacts (via phosphorylated form) with SFN.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pias2Q8C5D8-15EBI-7070449,EBI-8064899
Ube2iP632802EBI-7070449,EBI-80180

Protein-protein interaction databases

BioGridi199405. 6 interactions.
IntActiP08152. 5 interactions.
MINTiMINT-2828823.
STRINGi10090.ENSMUSP00000041053.

Structurei

3D structure databases

ProteinModelPortaliP08152.
SMRiP08152. Positions 334-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi167 – 1737Poly-Pro

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri337 – 36125C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri367 – 38923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri395 – 41723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00550000074455.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08152.
KOiK12496.
OMAiQKERKGS.
PhylomeDBiP08152.
TreeFamiTF318980.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P08152-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMTAKAVDKI PVTLSGFMHQ LPDSLYPVED LAASSVTIFP NGELGGPFDQ
60 70 80 90 100
MNGVAGDGMI NIDMTGEKRP LDLPYPSSFA PISAPRNQTF TYMGKFSIDP
110 120 130 140 150
QYPGASCYPE GIINIVSAGI LQGVTPPAST TASSSVTSAS PNPLATGPLG
160 170 180 190 200
VCTMSQTQPE LDHLYSPPPP PPPYSGCTGD LYQDPSAFLS PPSTTSTSSL
210 220 230 240 250
AYQPPPSYPS PKPAMDPGLI PMIPDYPGFF PSPCQRDPHG AAGPDRKPFP
260 270 280 290 300
CPLDSLRVPP PLTPLSTIRN FTLGGPGAGV TGPGASGGGE GPRLPGSGSA
310 320 330 340 350
AVTATPYNPH HLPLRPILRP RKYPNRPSKT PVHERPYPCP AEGCDRRFSR
360 370 380 390 400
SDELTRHIRI HTGHKPFQCR ICMRNFSRSD HLTTHIRTHT GEKPFACDYC
410 420 430 440 450
GRKFARSDER KRHTKIHLRQ KERKSSAPSA PPSAQSSASG PGGSQAGGSL
460 470
CGNSAIGGPL ASCTSRTRTP
Length:470
Mass (Da):49,819
Last modified:November 1, 1997 - v3
Checksum:i67712733C77960F0
GO
Isoform Short (identifier: P08152-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Show »
Length:420
Mass (Da):44,500
Checksum:i945C01B7DF6F20E3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5050Missing in isoform Short. 1 PublicationVSP_006864Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24377, M24376 Genomic DNA. Translation: AAA39379.1.
M24377, M24376 Genomic DNA. Translation: AAA39380.1.
X06746 mRNA. Translation: CAA29921.1.
BC009093 mRNA. Translation: AAH09093.1.
M20759 Genomic DNA. Translation: AAA39381.1.
CCDSiCCDS35927.2. [P08152-1]
PIRiA30136.
S00256.
RefSeqiNP_034248.2. NM_010118.3. [P08152-1]
XP_006513272.1. XM_006513209.1. [P08152-2]
XP_006513273.1. XM_006513210.1. [P08152-2]
XP_006513274.1. XM_006513211.1. [P08152-2]
XP_006513275.1. XM_006513212.1. [P08152-2]
XP_006513276.1. XM_006513213.2. [P08152-2]
UniGeneiMm.290421.

Genome annotation databases

EnsembliENSMUST00000048289; ENSMUSP00000041053; ENSMUSG00000037868. [P08152-1]
ENSMUST00000105438; ENSMUSP00000101078; ENSMUSG00000037868. [P08152-2]
GeneIDi13654.
KEGGimmu:13654.
UCSCiuc007flx.1. mouse. [P08152-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24377, M24376 Genomic DNA. Translation: AAA39379.1.
M24377, M24376 Genomic DNA. Translation: AAA39380.1.
X06746 mRNA. Translation: CAA29921.1.
BC009093 mRNA. Translation: AAH09093.1.
M20759 Genomic DNA. Translation: AAA39381.1.
CCDSiCCDS35927.2. [P08152-1]
PIRiA30136.
S00256.
RefSeqiNP_034248.2. NM_010118.3. [P08152-1]
XP_006513272.1. XM_006513209.1. [P08152-2]
XP_006513273.1. XM_006513210.1. [P08152-2]
XP_006513274.1. XM_006513211.1. [P08152-2]
XP_006513275.1. XM_006513212.1. [P08152-2]
XP_006513276.1. XM_006513213.2. [P08152-2]
UniGeneiMm.290421.

3D structure databases

ProteinModelPortaliP08152.
SMRiP08152. Positions 334-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199405. 6 interactions.
IntActiP08152. 5 interactions.
MINTiMINT-2828823.
STRINGi10090.ENSMUSP00000041053.

PTM databases

PhosphoSiteiP08152.

Proteomic databases

PRIDEiP08152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048289; ENSMUSP00000041053; ENSMUSG00000037868. [P08152-1]
ENSMUST00000105438; ENSMUSP00000101078; ENSMUSG00000037868. [P08152-2]
GeneIDi13654.
KEGGimmu:13654.
UCSCiuc007flx.1. mouse. [P08152-1]

Organism-specific databases

CTDi1959.
MGIiMGI:95296. Egr2.

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00550000074455.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08152.
KOiK12496.
OMAiQKERKGS.
PhylomeDBiP08152.
TreeFamiTF318980.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
REACT_309805. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

NextBioi284388.
PROiP08152.
SOURCEiSearch...

Gene expression databases

BgeeiP08152.
CleanExiMM_EGR2.
ExpressionAtlasiP08152. baseline and differential.
GenevisibleiP08152. MM.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos."
    Chavrier P., Janssen-Timmen U., Mattei M.-G., Zerial M., Bravo R., Charnay P.
    Mol. Cell. Biol. 9:787-797(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
  2. "A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells."
    Chavrier P., Zerial M., Lemaire P., Almendral J., Bravo R., Charnay P.
    EMBO J. 7:29-35(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Characterization of a mouse multigene family that encodes zinc finger structures."
    Chavrier P., Lemaire P., Revelant O., Bravo R., Charnay P.
    Mol. Cell. Biol. 8:1319-1326(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-417.
  5. "The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene."
    Chavrier P., Vesque C., Galliot B., Vigneron M., Dolle P., Duboule D., Charnay P.
    EMBO J. 9:1209-1218(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Krox-20: a candidate gene for the regulation of pattern formation in the hindbrain."
    Gilardi P., Schneider-Maunoury S., Charnay P.
    Biochimie 73:85-91(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Mapping functional regions of the segment-specific transcription factor Krox-20."
    Vesque C., Charnay P.
    Nucleic Acids Res. 20:2485-2492(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.
  8. "An EGR2/CITED1 transcription factor complex and the 14-3-3sigma tumor suppressor are involved in regulating ErbB2 expression in a transgenic-mouse model of human breast cancer."
    Dillon R.L., Brown S.T., Ling C., Shioda T., Muller W.J.
    Mol. Cell. Biol. 27:8648-8657(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CITED1 AND SFN, MUTAGENESIS OF SER-377, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  9. "The HECT-type E3 ubiquitin ligase AIP2 inhibits activation-induced T-cell death by catalyzing EGR2 ubiquitination."
    Chen A., Gao B., Zhang J., McEwen T., Ye S.Q., Zhang D., Fang D.
    Mol. Cell. Biol. 29:5348-5356(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, INTERACTION WITH WWP2, MUTAGENESIS OF TYR-174 AND TYR-208.

Entry informationi

Entry nameiEGR2_MOUSE
AccessioniPrimary (citable) accession number: P08152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.