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P08152

- EGR2_MOUSE

UniProt

P08152 - EGR2_MOUSE

Protein

E3 SUMO-protein ligase EGR2

Gene

Egr2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. Binds to the promoter region of ERBB2. May play a role in the regulation of hindbrain segmentation, might act in combination with the Hox network to specify odd and even rhombomeres, and might participate in the control of the expression of some of the homeobox containing genes.3 Publications
    E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri337 – 36125C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri367 – 38923C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri395 – 41723C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. DNA binding Source: MGI
    3. ligase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: UniProtKB
    6. RNA polymerase II activating transcription factor binding Source: BHF-UCL
    7. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    8. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. brain segmentation Source: MGI
    2. cellular response to cAMP Source: RefGenome
    3. cellular response to gonadotropin stimulus Source: RefGenome
    4. cellular response to organic substance Source: MGI
    5. facial nerve structural organization Source: MGI
    6. fat cell differentiation Source: BHF-UCL
    7. learning or memory Source: Ensembl
    8. motor neuron axon guidance Source: MGI
    9. myelination Source: MGI
    10. negative regulation of apoptotic process Source: RefGenome
    11. positive regulation of transcription, DNA-templated Source: UniProtKB
    12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    13. protein export from nucleus Source: UniProtKB
    14. protein sumoylation Source: UniProtKB-UniPathway
    15. regulation of neuronal synaptic plasticity Source: RefGenome
    16. regulation of ossification Source: MGI
    17. regulation of transcription, DNA-templated Source: MGI
    18. response to insulin Source: Ensembl
    19. rhombomere 3 development Source: MGI
    20. rhombomere 3 formation Source: MGI
    21. rhombomere 5 formation Source: MGI
    22. rhythmic behavior Source: MGI
    23. Schwann cell differentiation Source: MGI
    24. skeletal muscle cell differentiation Source: MGI
    25. transcription from RNA polymerase II promoter Source: BHF-UCL

    Keywords - Molecular functioni

    Activator, Ligase

    Keywords - Biological processi

    Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
    UniPathwayiUPA00886.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 SUMO-protein ligase EGR2 (EC:6.3.2.-)
    Alternative name(s):
    Early growth response protein 2
    Short name:
    EGR-2
    Zinc finger protein Krox-20
    Gene namesi
    Name:Egr2
    Synonyms:Egr-2, Krox-20, Zfp-25
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:95296. Egr2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi174 – 1741Y → F: Abolishes interaction with WWP2; if associated with F-208. 1 Publication
    Mutagenesisi208 – 2081Y → F: Abolishes interaction with WWP2; if associated with F-174. 1 Publication
    Mutagenesisi377 – 3771S → A: Inhibits interaction with SFN. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470E3 SUMO-protein ligase EGR2PRO_0000047120Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by WWP2 leading to proteasomal degradation.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiP08152.

    PTM databases

    PhosphoSiteiP08152.

    Expressioni

    Tissue specificityi

    Expressed in mammary tumors (at protein level). Expressed mainly in adult thymus and embryonic nervous system.1 Publication

    Inductioni

    Activated during G0/G1 transition in cultured cells.

    Gene expression databases

    ArrayExpressiP08152.
    BgeeiP08152.
    CleanExiMM_EGR2.
    GenevestigatoriP08152.

    Interactioni

    Subunit structurei

    Interacts with HCFC1. Interacts with UBC9 By similarity. Interacts with WWP2. Interacts with CITED1. Interacts (via phosphorylated form) with SFN.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Pias2Q8C5D8-15EBI-7070449,EBI-8064899
    Ube2iP632802EBI-7070449,EBI-80180

    Protein-protein interaction databases

    BioGridi199405. 6 interactions.
    IntActiP08152. 5 interactions.
    MINTiMINT-2828823.
    STRINGi10090.ENSMUSP00000041053.

    Structurei

    3D structure databases

    ProteinModelPortaliP08152.
    SMRiP08152. Positions 335-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi167 – 1737Poly-Pro

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri337 – 36125C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri367 – 38923C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri395 – 41723C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00550000074455.
    HOGENOMiHOG000036856.
    HOVERGENiHBG003909.
    InParanoidiP08152.
    KOiK12496.
    OMAiQKERKGS.
    PhylomeDBiP08152.
    TreeFamiTF318980.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR021849. DUF3446.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF11928. DUF3446. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P08152-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMTAKAVDKI PVTLSGFMHQ LPDSLYPVED LAASSVTIFP NGELGGPFDQ    50
    MNGVAGDGMI NIDMTGEKRP LDLPYPSSFA PISAPRNQTF TYMGKFSIDP 100
    QYPGASCYPE GIINIVSAGI LQGVTPPAST TASSSVTSAS PNPLATGPLG 150
    VCTMSQTQPE LDHLYSPPPP PPPYSGCTGD LYQDPSAFLS PPSTTSTSSL 200
    AYQPPPSYPS PKPAMDPGLI PMIPDYPGFF PSPCQRDPHG AAGPDRKPFP 250
    CPLDSLRVPP PLTPLSTIRN FTLGGPGAGV TGPGASGGGE GPRLPGSGSA 300
    AVTATPYNPH HLPLRPILRP RKYPNRPSKT PVHERPYPCP AEGCDRRFSR 350
    SDELTRHIRI HTGHKPFQCR ICMRNFSRSD HLTTHIRTHT GEKPFACDYC 400
    GRKFARSDER KRHTKIHLRQ KERKSSAPSA PPSAQSSASG PGGSQAGGSL 450
    CGNSAIGGPL ASCTSRTRTP 470
    Length:470
    Mass (Da):49,819
    Last modified:November 1, 1997 - v3
    Checksum:i67712733C77960F0
    GO
    Isoform Short (identifier: P08152-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-50: Missing.

    Show »
    Length:420
    Mass (Da):44,500
    Checksum:i945C01B7DF6F20E3
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5050Missing in isoform Short. 1 PublicationVSP_006864Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24377, M24376 Genomic DNA. Translation: AAA39379.1.
    M24377, M24376 Genomic DNA. Translation: AAA39380.1.
    X06746 mRNA. Translation: CAA29921.1.
    BC009093 mRNA. Translation: AAH09093.1.
    M20759 Genomic DNA. Translation: AAA39381.1.
    CCDSiCCDS35927.2. [P08152-1]
    PIRiA30136.
    S00256.
    RefSeqiNP_034248.2. NM_010118.3. [P08152-1]
    XP_006513272.1. XM_006513209.1. [P08152-2]
    XP_006513273.1. XM_006513210.1. [P08152-2]
    XP_006513274.1. XM_006513211.1. [P08152-2]
    XP_006513275.1. XM_006513212.1. [P08152-2]
    XP_006513276.1. XM_006513213.1. [P08152-2]
    UniGeneiMm.290421.

    Genome annotation databases

    EnsembliENSMUST00000048289; ENSMUSP00000041053; ENSMUSG00000037868. [P08152-1]
    ENSMUST00000105438; ENSMUSP00000101078; ENSMUSG00000037868. [P08152-2]
    GeneIDi13654.
    KEGGimmu:13654.
    UCSCiuc007flx.1. mouse. [P08152-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24377 , M24376 Genomic DNA. Translation: AAA39379.1 .
    M24377 , M24376 Genomic DNA. Translation: AAA39380.1 .
    X06746 mRNA. Translation: CAA29921.1 .
    BC009093 mRNA. Translation: AAH09093.1 .
    M20759 Genomic DNA. Translation: AAA39381.1 .
    CCDSi CCDS35927.2. [P08152-1 ]
    PIRi A30136.
    S00256.
    RefSeqi NP_034248.2. NM_010118.3. [P08152-1 ]
    XP_006513272.1. XM_006513209.1. [P08152-2 ]
    XP_006513273.1. XM_006513210.1. [P08152-2 ]
    XP_006513274.1. XM_006513211.1. [P08152-2 ]
    XP_006513275.1. XM_006513212.1. [P08152-2 ]
    XP_006513276.1. XM_006513213.1. [P08152-2 ]
    UniGenei Mm.290421.

    3D structure databases

    ProteinModelPortali P08152.
    SMRi P08152. Positions 335-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199405. 6 interactions.
    IntActi P08152. 5 interactions.
    MINTi MINT-2828823.
    STRINGi 10090.ENSMUSP00000041053.

    PTM databases

    PhosphoSitei P08152.

    Proteomic databases

    PRIDEi P08152.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000048289 ; ENSMUSP00000041053 ; ENSMUSG00000037868 . [P08152-1 ]
    ENSMUST00000105438 ; ENSMUSP00000101078 ; ENSMUSG00000037868 . [P08152-2 ]
    GeneIDi 13654.
    KEGGi mmu:13654.
    UCSCi uc007flx.1. mouse. [P08152-1 ]

    Organism-specific databases

    CTDi 1959.
    MGIi MGI:95296. Egr2.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00550000074455.
    HOGENOMi HOG000036856.
    HOVERGENi HBG003909.
    InParanoidi P08152.
    KOi K12496.
    OMAi QKERKGS.
    PhylomeDBi P08152.
    TreeFami TF318980.

    Enzyme and pathway databases

    UniPathwayi UPA00886 .
    Reactomei REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 284388.
    PROi P08152.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08152.
    Bgeei P08152.
    CleanExi MM_EGR2.
    Genevestigatori P08152.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR021849. DUF3446.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF11928. DUF3446. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos."
      Chavrier P., Janssen-Timmen U., Mattei M.-G., Zerial M., Bravo R., Charnay P.
      Mol. Cell. Biol. 9:787-797(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
    2. "A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells."
      Chavrier P., Zerial M., Lemaire P., Almendral J., Bravo R., Charnay P.
      EMBO J. 7:29-35(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "Characterization of a mouse multigene family that encodes zinc finger structures."
      Chavrier P., Lemaire P., Revelant O., Bravo R., Charnay P.
      Mol. Cell. Biol. 8:1319-1326(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-417.
    5. "The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene."
      Chavrier P., Vesque C., Galliot B., Vigneron M., Dolle P., Duboule D., Charnay P.
      EMBO J. 9:1209-1218(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Krox-20: a candidate gene for the regulation of pattern formation in the hindbrain."
      Gilardi P., Schneider-Maunoury S., Charnay P.
      Biochimie 73:85-91(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Mapping functional regions of the segment-specific transcription factor Krox-20."
      Vesque C., Charnay P.
      Nucleic Acids Res. 20:2485-2492(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAINS.
    8. "An EGR2/CITED1 transcription factor complex and the 14-3-3sigma tumor suppressor are involved in regulating ErbB2 expression in a transgenic-mouse model of human breast cancer."
      Dillon R.L., Brown S.T., Ling C., Shioda T., Muller W.J.
      Mol. Cell. Biol. 27:8648-8657(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CITED1 AND SFN, MUTAGENESIS OF SER-377, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    9. "The HECT-type E3 ubiquitin ligase AIP2 inhibits activation-induced T-cell death by catalyzing EGR2 ubiquitination."
      Chen A., Gao B., Zhang J., McEwen T., Ye S.Q., Zhang D., Fang D.
      Mol. Cell. Biol. 29:5348-5356(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION, INTERACTION WITH WWP2, MUTAGENESIS OF TYR-174 AND TYR-208.

    Entry informationi

    Entry nameiEGR2_MOUSE
    AccessioniPrimary (citable) accession number: P08152
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 147 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3