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P08152 (EGR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 SUMO-protein ligase EGR2

EC=6.3.2.-
Alternative name(s):
Early growth response protein 2
Short name=EGR-2
Zinc finger protein Krox-20
Gene names
Name:Egr2
Synonyms:Egr-2, Krox-20, Zfp-25
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. Binds to the promoter region of ERBB2. May play a role in the regulation of hindbrain segmentation, might act in combination with the Hox network to specify odd and even rhombomeres, and might participate in the control of the expression of some of the homeobox containing genes. Ref.5 Ref.6 Ref.8

E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity By similarity. Ref.5 Ref.6 Ref.8

Pathway

Protein modification; protein sumoylation.

Subunit structure

Interacts with HCFC1. Interacts with UBC9 By similarity. Interacts with WWP2. Interacts with CITED1. Interacts (via phosphorylated form) with SFN. Ref.8 Ref.9

Subcellular location

Nucleus Ref.8.

Tissue specificity

Expressed in mammary tumors (at protein level). Expressed mainly in adult thymus and embryonic nervous system. Ref.8

Induction

Activated during G0/G1 transition in cultured cells.

Post-translational modification

Ubiquitinated by WWP2 leading to proteasomal degradation. Ref.9

Sequence similarities

Belongs to the EGR C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
Ubl conjugation pathway
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Ligase
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSchwann cell differentiation

Inferred from mutant phenotype PubMed 16136673. Source: MGI

brain segmentation

Inferred from mutant phenotype PubMed 7903221. Source: MGI

cellular response to cAMP

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to gonadotropin stimulus

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to organic substance

Inferred from direct assay PubMed 21357543. Source: MGI

facial nerve structural organization

Inferred from genetic interaction PubMed 9806922. Source: MGI

fat cell differentiation

Inferred from mutant phenotype PubMed 18396140. Source: BHF-UCL

learning or memory

Inferred from electronic annotation. Source: Ensembl

motor neuron axon guidance

Inferred from genetic interaction PubMed 9806922. Source: MGI

myelination

Inferred from mutant phenotype PubMed 10704452PubMed 16136673. Source: MGI

negative regulation of apoptotic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 8093858. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.8. Source: UniProtKB

protein export from nucleus

Inferred from direct assay Ref.8. Source: UniProtKB

protein sumoylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

regulation of neuronal synaptic plasticity

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of ossification

Inferred from mutant phenotype PubMed 8565822. Source: MGI

regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 10704452PubMed 16136673. Source: MGI

response to insulin

Inferred from electronic annotation. Source: Ensembl

rhombomere 3 development

Inferred from genetic interaction PubMed 9806922. Source: MGI

rhombomere 3 formation

Inferred from mutant phenotype PubMed 7903221. Source: MGI

rhombomere 5 formation

Inferred from mutant phenotype PubMed 7903221. Source: MGI

rhythmic behavior

Inferred from direct assay PubMed 8893031. Source: MGI

skeletal muscle cell differentiation

Inferred from mutant phenotype PubMed 22147266. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18396140. Source: BHF-UCL

   Cellular_componentcytoplasm

Inferred from direct assay Ref.8. Source: UniProtKB

nucleus

Inferred from direct assay Ref.8. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay PubMed 8093858PubMed 8895582. Source: MGI

RNA polymerase II activating transcription factor binding

Inferred from physical interaction PubMed 18396140. Source: BHF-UCL

chromatin binding

Inferred from direct assay Ref.8. Source: UniProtKB

ligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.8. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from direct assay Ref.8. Source: UniProtKB

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P08152-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P08152-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470E3 SUMO-protein ligase EGR2
PRO_0000047120

Regions

Zinc finger337 – 36125C2H2-type 1
Zinc finger367 – 38923C2H2-type 2
Zinc finger395 – 41723C2H2-type 3
Compositional bias167 – 1737Poly-Pro

Natural variations

Alternative sequence1 – 5050Missing in isoform Short.
VSP_006864

Experimental info

Mutagenesis1741Y → F: Abolishes interaction with WWP2; if associated with F-208. Ref.9
Mutagenesis2081Y → F: Abolishes interaction with WWP2; if associated with F-174. Ref.9
Mutagenesis3771S → A: Inhibits interaction with SFN. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 67712733C77960F0

FASTA47049,819
        10         20         30         40         50         60 
MMTAKAVDKI PVTLSGFMHQ LPDSLYPVED LAASSVTIFP NGELGGPFDQ MNGVAGDGMI 

        70         80         90        100        110        120 
NIDMTGEKRP LDLPYPSSFA PISAPRNQTF TYMGKFSIDP QYPGASCYPE GIINIVSAGI 

       130        140        150        160        170        180 
LQGVTPPAST TASSSVTSAS PNPLATGPLG VCTMSQTQPE LDHLYSPPPP PPPYSGCTGD 

       190        200        210        220        230        240 
LYQDPSAFLS PPSTTSTSSL AYQPPPSYPS PKPAMDPGLI PMIPDYPGFF PSPCQRDPHG 

       250        260        270        280        290        300 
AAGPDRKPFP CPLDSLRVPP PLTPLSTIRN FTLGGPGAGV TGPGASGGGE GPRLPGSGSA 

       310        320        330        340        350        360 
AVTATPYNPH HLPLRPILRP RKYPNRPSKT PVHERPYPCP AEGCDRRFSR SDELTRHIRI 

       370        380        390        400        410        420 
HTGHKPFQCR ICMRNFSRSD HLTTHIRTHT GEKPFACDYC GRKFARSDER KRHTKIHLRQ 

       430        440        450        460        470 
KERKSSAPSA PPSAQSSASG PGGSQAGGSL CGNSAIGGPL ASCTSRTRTP 

« Hide

Isoform Short [UniParc].

Checksum: 945C01B7DF6F20E3
Show »

FASTA42044,500

References

« Hide 'large scale' references
[1]"Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos."
Chavrier P., Janssen-Timmen U., Mattei M.-G., Zerial M., Bravo R., Charnay P.
Mol. Cell. Biol. 9:787-797(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
[2]"A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells."
Chavrier P., Zerial M., Lemaire P., Almendral J., Bravo R., Charnay P.
EMBO J. 7:29-35(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"Characterization of a mouse multigene family that encodes zinc finger structures."
Chavrier P., Lemaire P., Revelant O., Bravo R., Charnay P.
Mol. Cell. Biol. 8:1319-1326(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-417.
[5]"The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene."
Chavrier P., Vesque C., Galliot B., Vigneron M., Dolle P., Duboule D., Charnay P.
EMBO J. 9:1209-1218(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Krox-20: a candidate gene for the regulation of pattern formation in the hindbrain."
Gilardi P., Schneider-Maunoury S., Charnay P.
Biochimie 73:85-91(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Mapping functional regions of the segment-specific transcription factor Krox-20."
Vesque C., Charnay P.
Nucleic Acids Res. 20:2485-2492(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAINS.
[8]"An EGR2/CITED1 transcription factor complex and the 14-3-3sigma tumor suppressor are involved in regulating ErbB2 expression in a transgenic-mouse model of human breast cancer."
Dillon R.L., Brown S.T., Ling C., Shioda T., Muller W.J.
Mol. Cell. Biol. 27:8648-8657(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CITED1 AND SFN, MUTAGENESIS OF SER-377, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]"The HECT-type E3 ubiquitin ligase AIP2 inhibits activation-induced T-cell death by catalyzing EGR2 ubiquitination."
Chen A., Gao B., Zhang J., McEwen T., Ye S.Q., Zhang D., Fang D.
Mol. Cell. Biol. 29:5348-5356(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION, INTERACTION WITH WWP2, MUTAGENESIS OF TYR-174 AND TYR-208.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24377, M24376 Genomic DNA. Translation: AAA39379.1.
M24377, M24376 Genomic DNA. Translation: AAA39380.1.
X06746 mRNA. Translation: CAA29921.1.
BC009093 mRNA. Translation: AAH09093.1.
M20759 Genomic DNA. Translation: AAA39381.1.
CCDSCCDS35927.2. [P08152-1]
PIRA30136.
S00256.
RefSeqNP_034248.2. NM_010118.3. [P08152-1]
XP_006513272.1. XM_006513209.1. [P08152-2]
XP_006513273.1. XM_006513210.1. [P08152-2]
XP_006513274.1. XM_006513211.1. [P08152-2]
XP_006513275.1. XM_006513212.1. [P08152-2]
XP_006513276.1. XM_006513213.1. [P08152-2]
UniGeneMm.290421.

3D structure databases

ProteinModelPortalP08152.
SMRP08152. Positions 335-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199405. 6 interactions.
IntActP08152. 5 interactions.
MINTMINT-2828823.
STRING10090.ENSMUSP00000041053.

PTM databases

PhosphoSiteP08152.

Proteomic databases

PRIDEP08152.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048289; ENSMUSP00000041053; ENSMUSG00000037868. [P08152-1]
ENSMUST00000105438; ENSMUSP00000101078; ENSMUSG00000037868. [P08152-2]
GeneID13654.
KEGGmmu:13654.
UCSCuc007flx.1. mouse. [P08152-1]

Organism-specific databases

CTD1959.
MGIMGI:95296. Egr2.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00550000074455.
HOGENOMHOG000036856.
HOVERGENHBG003909.
InParanoidP08152.
KOK12496.
OMAQKERKGS.
PhylomeDBP08152.
TreeFamTF318980.

Enzyme and pathway databases

UniPathwayUPA00886.

Gene expression databases

ArrayExpressP08152.
BgeeP08152.
CleanExMM_EGR2.
GenevestigatorP08152.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF11928. DUF3446. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio284388.
PROP08152.
SOURCESearch...

Entry information

Entry nameEGR2_MOUSE
AccessionPrimary (citable) accession number: P08152
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot