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P08149

- PBP2_NEIGO

UniProt

P08149 - PBP2_NEIGO

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Protein

Penicillin-binding protein 2

Gene

penA

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Synthesis of cross-linked peptidoglycan from the lipid intermediates.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei310 – 3101Acyl-ester intermediate

GO - Molecular functioni

  1. catalytic activity Source: UniProtKB-KW
  2. penicillin binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  5. regulation of cell shape Source: UniProtKB-KW
  6. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2
Short name:
PBP-2
Gene namesi
Name:penA
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Penicillin-binding protein 2PRO_0000195449Add
BLAST

Structurei

Secondary structure

1
581
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi55 – 573
Beta strandi65 – 717
Beta strandi85 – 917
Beta strandi165 – 1684
Helixi172 – 1754
Helixi176 – 1794
Beta strandi188 – 1903
Helixi191 – 1955
Helixi197 – 2015
Beta strandi205 – 2117
Beta strandi217 – 2204
Beta strandi235 – 2384
Helixi241 – 25717
Beta strandi261 – 2699
Turni270 – 2723
Beta strandi274 – 2829
Helixi289 – 2913
Helixi294 – 2974
Helixi300 – 3034
Helixi309 – 3124
Helixi313 – 32210
Beta strandi331 – 3333
Beta strandi337 – 3393
Beta strandi342 – 3443
Beta strandi351 – 3544
Helixi355 – 3617
Helixi364 – 3718
Helixi376 – 38510
Turni386 – 3894
Helixi407 – 4093
Helixi412 – 4198
Helixi428 – 43912
Turni440 – 4423
Beta strandi448 – 4503
Helixi464 – 47512
Turni476 – 4783
Helixi485 – 4873
Beta strandi495 – 5006
Beta strandi515 – 52612
Beta strandi529 – 5379
Helixi545 – 5484
Helixi550 – 56314

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EQUX-ray2.40A/B44-581[»]
3EQVX-ray2.40A/B44-581[»]
ProteinModelPortaliP08149.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08149.

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 4821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domaini

The enzyme has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Sequence similaritiesi

Belongs to the transpeptidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

P08149-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLIKSEYKPR MLPKEEQVKK PMTSNGRISF VLMAMAVLFA CLIARGLYLQ
60 70 80 90 100
TVTYNFLKEQ GDNRIVRTQA LPATRGTVSD RNGAVLALSA PTESLFAVPK
110 120 130 140 150
DMKEMPSAAQ LERLSELVDV PVDVLRNKLE QKGKSFIWIK RQLDPKVAEE
160 170 180 190 200
VKALGLENFV FEKELKRHYP MGNLFAHVIG FTDIDGKGQE GLELSLEDSL
210 220 230 240 250
YGEDGAEVVL RDRQGNIVDS LDSPRNKAPQ NGKDIILSLD QRIQTLAYEE
260 270 280 290 300
LNKAVEYHQA KAGTVVVLDA RTGEILALAN TPAYDPNRPG RADSEQRRNR
310 320 330 340 350
AVTDMIEPGS AIKPFVIAKA LDAGKTDLNE RLNTQPYKIG PSPVRDTHVY
360 370 380 390 400
PSLDVRGIMQ KSSNVGTSKL SARFGAEEMY DFYHELGIGV RMHSGFPGET
410 420 430 440 450
AGLLRNWRRW RPIEQATMSF GYGLQLSLLQ LARAYTALTH DGVLLPLSFE
460 470 480 490 500
KQAVAPQGKR IFKESTAREV RNLMVSVTEP GGTGTAGAVD GFDVGAKTGT
510 520 530 540 550
ARKFVNGRYA DNKHVATFIG FAPAKNPRVI VAVTIDEPTA HGYYGGVVAG
560 570 580
PPFKKIMGGS LNILGISPTK PLTAAAVKTP S
Length:581
Mass (Da):63,650
Last modified:August 1, 1988 - v1
Checksum:iA495A788E465EFA2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti346 – 3461D → DD in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural varianti504 – 5041F → L in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural varianti510 – 5101A → V in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural varianti516 – 5161A → G in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural varianti541 – 5411H → N in strain: FA19 and CDC84-060418.
Natural varianti551 – 5511P → L in strain: CDC84-060384.
Natural varianti551 – 5511P → S in strain: CDC77-124615.
Natural varianti552 – 5521P → V in strain: CDC84-060418.
Natural varianti555 – 5562KI → QV in strain: CDC84-060418.
Natural varianti566 – 5661I → V in strain: CDC84-060418.
Natural varianti574 – 5741A → NV in strain: CDC84-060418.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32091 Genomic DNA. Translation: AAA25463.1.
X07468 Genomic DNA. Translation: CAA30356.1.
X07469 Genomic DNA. Translation: CAA30357.1.
X07470 Genomic DNA. Translation: CAA30358.1.
PIRiS00916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32091 Genomic DNA. Translation: AAA25463.1 .
X07468 Genomic DNA. Translation: CAA30356.1 .
X07469 Genomic DNA. Translation: CAA30357.1 .
X07470 Genomic DNA. Translation: CAA30358.1 .
PIRi S00916.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3EQU X-ray 2.40 A/B 44-581 [» ]
3EQV X-ray 2.40 A/B 44-581 [» ]
ProteinModelPortali P08149.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB00535. Cefdinir.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00219 .

Miscellaneous databases

EvolutionaryTracei P08149.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae."
    Spratt B.G.
    Nature 332:173-176(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPBP2_NEIGO
AccessioniPrimary (citable) accession number: P08149
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein was sequenced in penicillin-sensitive strains LM306, and FA19, and in penicillin-resistant strains CDC84-060384, CDC84-060418 and CDC77-124615. The sequence shown is that of strain LM306.

Keywords - Technical termi

3D-structure, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3