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P08149

- PBP2_NEIGO

UniProt

P08149 - PBP2_NEIGO

Protein

Penicillin-binding protein 2

Gene

penA

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Synthesis of cross-linked peptidoglycan from the lipid intermediates.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei310 – 3101Acyl-ester intermediate

    GO - Molecular functioni

    1. catalytic activity Source: UniProtKB-KW
    2. penicillin binding Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
    4. regulation of cell shape Source: UniProtKB-KW
    5. response to antibiotic Source: UniProtKB-KW

    Keywords - Biological processi

    Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 2
    Short name:
    PBP-2
    Gene namesi
    Name:penA
    OrganismiNeisseria gonorrhoeae
    Taxonomic identifieri485 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Penicillin-binding protein 2PRO_0000195449Add
    BLAST

    Structurei

    Secondary structure

    1
    581
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi55 – 573
    Beta strandi65 – 717
    Beta strandi85 – 917
    Beta strandi165 – 1684
    Helixi172 – 1754
    Helixi176 – 1794
    Beta strandi188 – 1903
    Helixi191 – 1955
    Helixi197 – 2015
    Beta strandi205 – 2117
    Beta strandi217 – 2204
    Beta strandi235 – 2384
    Helixi241 – 25717
    Beta strandi261 – 2699
    Turni270 – 2723
    Beta strandi274 – 2829
    Helixi289 – 2913
    Helixi294 – 2974
    Helixi300 – 3034
    Helixi309 – 3124
    Helixi313 – 32210
    Beta strandi331 – 3333
    Beta strandi337 – 3393
    Beta strandi342 – 3443
    Beta strandi351 – 3544
    Helixi355 – 3617
    Helixi364 – 3718
    Helixi376 – 38510
    Turni386 – 3894
    Helixi407 – 4093
    Helixi412 – 4198
    Helixi428 – 43912
    Turni440 – 4423
    Beta strandi448 – 4503
    Helixi464 – 47512
    Turni476 – 4783
    Helixi485 – 4873
    Beta strandi495 – 5006
    Beta strandi515 – 52612
    Beta strandi529 – 5379
    Helixi545 – 5484
    Helixi550 – 56314

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3EQUX-ray2.40A/B44-581[»]
    3EQVX-ray2.40A/B44-581[»]
    ProteinModelPortaliP08149.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08149.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 4821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The enzyme has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

    Sequence similaritiesi

    Belongs to the transpeptidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P08149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIKSEYKPR MLPKEEQVKK PMTSNGRISF VLMAMAVLFA CLIARGLYLQ    50
    TVTYNFLKEQ GDNRIVRTQA LPATRGTVSD RNGAVLALSA PTESLFAVPK 100
    DMKEMPSAAQ LERLSELVDV PVDVLRNKLE QKGKSFIWIK RQLDPKVAEE 150
    VKALGLENFV FEKELKRHYP MGNLFAHVIG FTDIDGKGQE GLELSLEDSL 200
    YGEDGAEVVL RDRQGNIVDS LDSPRNKAPQ NGKDIILSLD QRIQTLAYEE 250
    LNKAVEYHQA KAGTVVVLDA RTGEILALAN TPAYDPNRPG RADSEQRRNR 300
    AVTDMIEPGS AIKPFVIAKA LDAGKTDLNE RLNTQPYKIG PSPVRDTHVY 350
    PSLDVRGIMQ KSSNVGTSKL SARFGAEEMY DFYHELGIGV RMHSGFPGET 400
    AGLLRNWRRW RPIEQATMSF GYGLQLSLLQ LARAYTALTH DGVLLPLSFE 450
    KQAVAPQGKR IFKESTAREV RNLMVSVTEP GGTGTAGAVD GFDVGAKTGT 500
    ARKFVNGRYA DNKHVATFIG FAPAKNPRVI VAVTIDEPTA HGYYGGVVAG 550
    PPFKKIMGGS LNILGISPTK PLTAAAVKTP S 581
    Length:581
    Mass (Da):63,650
    Last modified:August 1, 1988 - v1
    Checksum:iA495A788E465EFA2
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti346 – 3461D → DD in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
    Natural varianti504 – 5041F → L in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
    Natural varianti510 – 5101A → V in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
    Natural varianti516 – 5161A → G in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
    Natural varianti541 – 5411H → N in strain: FA19 and CDC84-060418.
    Natural varianti551 – 5511P → L in strain: CDC84-060384.
    Natural varianti551 – 5511P → S in strain: CDC77-124615.
    Natural varianti552 – 5521P → V in strain: CDC84-060418.
    Natural varianti555 – 5562KI → QV in strain: CDC84-060418.
    Natural varianti566 – 5661I → V in strain: CDC84-060418.
    Natural varianti574 – 5741A → NV in strain: CDC84-060418.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32091 Genomic DNA. Translation: AAA25463.1.
    X07468 Genomic DNA. Translation: CAA30356.1.
    X07469 Genomic DNA. Translation: CAA30357.1.
    X07470 Genomic DNA. Translation: CAA30358.1.
    PIRiS00916.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32091 Genomic DNA. Translation: AAA25463.1 .
    X07468 Genomic DNA. Translation: CAA30356.1 .
    X07469 Genomic DNA. Translation: CAA30357.1 .
    X07470 Genomic DNA. Translation: CAA30358.1 .
    PIRi S00916.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3EQU X-ray 2.40 A/B 44-581 [» ]
    3EQV X-ray 2.40 A/B 44-581 [» ]
    ProteinModelPortali P08149.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00219 .

    Miscellaneous databases

    EvolutionaryTracei P08149.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae."
      Spratt B.G.
      Nature 332:173-176(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPBP2_NEIGO
    AccessioniPrimary (citable) accession number: P08149
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein was sequenced in penicillin-sensitive strains LM306, and FA19, and in penicillin-resistant strains CDC84-060384, CDC84-060418 and CDC77-124615. The sequence shown is that of strain LM306.

    Keywords - Technical termi

    3D-structure, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3