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P08149 (PBP2_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Penicillin-binding protein 2
Short name=PBP-2
Gene names
Name:penA
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Synthesis of cross-linked peptidoglycan from the lipid intermediates.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell inner membrane; Peripheral membrane protein.

Domain

The enzyme has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Miscellaneous

This protein was sequenced in penicillin-sensitive strains LM306, and FA19, and in penicillin-resistant strains CDC84-060384, CDC84-060418 and CDC77-124615. The sequence shown is that of strain LM306.

Sequence similarities

Belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 581581Penicillin-binding protein 2
PRO_0000195449

Sites

Active site3101Acyl-ester intermediate

Natural variations

Natural variant3461D → DD in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural variant5041F → L in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural variant5101A → V in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural variant5161A → G in strain: CDC84-060418, CDC77-124615 and CDC84-060384.
Natural variant5411H → N in strain: FA19 and CDC84-060418.
Natural variant5511P → L in strain: CDC84-060384.
Natural variant5511P → S in strain: CDC77-124615.
Natural variant5521P → V in strain: CDC84-060418.
Natural variant555 – 5562KI → QV in strain: CDC84-060418.
Natural variant5661I → V in strain: CDC84-060418.
Natural variant5741A → NV in strain: CDC84-060418.

Secondary structure

1 ............................................................................. 581
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08149 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: A495A788E465EFA2

FASTA58163,650
        10         20         30         40         50         60 
MLIKSEYKPR MLPKEEQVKK PMTSNGRISF VLMAMAVLFA CLIARGLYLQ TVTYNFLKEQ 

        70         80         90        100        110        120 
GDNRIVRTQA LPATRGTVSD RNGAVLALSA PTESLFAVPK DMKEMPSAAQ LERLSELVDV 

       130        140        150        160        170        180 
PVDVLRNKLE QKGKSFIWIK RQLDPKVAEE VKALGLENFV FEKELKRHYP MGNLFAHVIG 

       190        200        210        220        230        240 
FTDIDGKGQE GLELSLEDSL YGEDGAEVVL RDRQGNIVDS LDSPRNKAPQ NGKDIILSLD 

       250        260        270        280        290        300 
QRIQTLAYEE LNKAVEYHQA KAGTVVVLDA RTGEILALAN TPAYDPNRPG RADSEQRRNR 

       310        320        330        340        350        360 
AVTDMIEPGS AIKPFVIAKA LDAGKTDLNE RLNTQPYKIG PSPVRDTHVY PSLDVRGIMQ 

       370        380        390        400        410        420 
KSSNVGTSKL SARFGAEEMY DFYHELGIGV RMHSGFPGET AGLLRNWRRW RPIEQATMSF 

       430        440        450        460        470        480 
GYGLQLSLLQ LARAYTALTH DGVLLPLSFE KQAVAPQGKR IFKESTAREV RNLMVSVTEP 

       490        500        510        520        530        540 
GGTGTAGAVD GFDVGAKTGT ARKFVNGRYA DNKHVATFIG FAPAKNPRVI VAVTIDEPTA 

       550        560        570        580 
HGYYGGVVAG PPFKKIMGGS LNILGISPTK PLTAAAVKTP S

« Hide

References

[1]"Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae."
Spratt B.G.
Nature 332:173-176(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M32091 Genomic DNA. Translation: AAA25463.1.
X07468 Genomic DNA. Translation: CAA30356.1.
X07469 Genomic DNA. Translation: CAA30357.1.
X07470 Genomic DNA. Translation: CAA30358.1.
PIRS00916.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3EQUX-ray2.40A/B44-581[»]
3EQVX-ray2.40A/B44-581[»]
ProteinModelPortalP08149.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56519. PBP_dimer. 1 hit.
SSF56601. PBP_transp_fold. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP08149.

Entry information

Entry namePBP2_NEIGO
AccessionPrimary (citable) accession number: P08149
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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