##gff-version 3
P08144	UniProtKB	Signal peptide	1	18	.	.	.	.	
P08144	UniProtKB	Chain	19	494	.	.	.	ID=PRO_0000001364;Note=Alpha-amylase A	
P08144	UniProtKB	Active site	204	204	.	.	.	Note=Nucleophile;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Active site	241	241	.	.	.	Note=Proton donor;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	116	116	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	165	165	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	174	174	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	202	202	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	208	208	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	304	304	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Binding site	343	343	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Site	306	306	.	.	.	Note=Transition state stabilizer;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Modified residue	19	19	.	.	.	Note=Pyrrolidone carboxylic acid;Ontology_term=ECO:0000250;evidence=ECO:0000250	
P08144	UniProtKB	Disulfide bond	46	102	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Disulfide bond	153	167	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Disulfide bond	376	382	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Disulfide bond	448	460	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P04746	
P08144	UniProtKB	Natural variant	11	11	.	.	.	Note=In strain: KN-28. A->S	
P08144	UniProtKB	Natural variant	16	16	.	.	.	Note=In strain: KN-22 and KN-23. A->V	
P08144	UniProtKB	Natural variant	71	71	.	.	.	Note=In strain: KN-3%2C KN-9%2C KN-10 and L16. S->R	
P08144	UniProtKB	Natural variant	121	121	.	.	.	Note=In strain: TN256%2C 1420#1 and KO140. D->G	
P08144	UniProtKB	Natural variant	121	121	.	.	.	Note=In strain: KN-3%2C KN-9%2C KN-10 and L16. D->N	
P08144	UniProtKB	Natural variant	138	138	.	.	.	Note=In strain: JP-75%2C KN-7%2C KN-15%2C KN-17%2C KN-21 and KN-23. S->T	
P08144	UniProtKB	Natural variant	144	144	.	.	.	Note=In strain: Canton-S. Y->H	
P08144	UniProtKB	Natural variant	156	156	.	.	.	Note=In strain: 1420#1%2C KN-3%2C KN-9%2C KN-10%2C KO140%2C L16 and TN256. S->R	
P08144	UniProtKB	Natural variant	181	181	.	.	.	Note=In strain: Canton-S. Y->N	
P08144	UniProtKB	Natural variant	231	231	.	.	.	Note=In strain: JP-70%2C KN-17 and KN-21. A->S	
P08144	UniProtKB	Natural variant	278	278	.	.	.	Note=In strain: 1420#1%2C KO140%2C KN-3%2C KN-9%2C KN-10 and L16. D->N	
P08144	UniProtKB	Natural variant	284	284	.	.	.	Note=In strain: KN-21. T->I	
P08144	UniProtKB	Natural variant	398	398	.	.	.	Note=In strain: 1420#1%2C J87%2C JP-60%2C JP-70%2C JP-75%2C KO123%2C KO140%2C KN-9%2C KN-15%2C KN-21%2C L16 and TN256. T->A	
P08144	UniProtKB	Natural variant	401	401	.	.	.	Note=In strain: Berkeley. S->L	
P08144	UniProtKB	Natural variant	403	403	.	.	.	Note=In strain: J87%2C JP-60 and KO123. E->A	
P08144	UniProtKB	Natural variant	465	465	.	.	.	Note=In strain: 1420#1. V->I	
P08144	UniProtKB	Natural variant	474	474	.	.	.	Note=In strain: KN-23. S->Y	
P08144	UniProtKB	Natural variant	476	476	.	.	.	Note=In strain: Berkeley%2C JP-1%2C JP-5%2C JP-15%2C JP-35%2C JP-55%2C JP-65%2C JP-84%2C KN-12%2C KN-22 and KN-27. N->Y