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Protein

Alpha-amylase A

Gene

Amy-p

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116CalciumBy similarity1
Metal bindingi165Calcium; via carbonyl oxygenBy similarity1
Metal bindingi174CalciumBy similarity1
Binding sitei202ChlorideBy similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium; via carbonyl oxygenBy similarity1
Active sitei241Proton donorBy similarity1
Binding sitei304ChlorideBy similarity1
Sitei306Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: UniProtKB
  • alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
  • calcium ion binding Source: UniProtKB

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB
  • sleep Source: FlyBase

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-189085. Digestion of dietary carbohydrate.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase A (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-p
Synonyms:AmyA
ORF Names:CG18730
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000079. Amy-p.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000000136419 – 494Alpha-amylase AAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP08144.
PeptideAtlasiP08144.
PRIDEiP08144.

Expressioni

Gene expression databases

BgeeiFBgn0000079.
ExpressionAtlasiP08144. differential.
GenevisibleiP08144. DM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi70922. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP08144.
SMRiP08144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
InParanoidiP08144.
KOiK01176.
OrthoDBiEOG091G07V6.
PhylomeDBiP08144.

Family and domain databases

InterProiView protein in InterPro
IPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPNGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEEQFASMVK
110 120 130 140 150
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPYSSLDFN
160 170 180 190 200
PTCAISNYND ANEVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLAVIYGR LKNLNTDHGF ASGSKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA ITEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
TDTDQGPPTT DGHNIASPIF NSDNSCSGGW VCEHRWRQIY NMVAFRNTVG
410 420 430 440 450
SDEIQNWWDN GSNQISFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVTVGSD GRASINIGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,746
Last modified:August 1, 1988 - v1
Checksum:iB477D3B44754C298
GO

Polymorphismi

At least 6 electrophoretic isozymes are known: Amy1, Amy2, Amy3, Amy4, Amy5 and Amy6. Strains J87 and KO123 express Amy2; KO140 and 1420#1 express Amy4; L16 expresses Amy5.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti11A → S in strain: KN-28. 1
Natural varianti16A → V in strain: KN-22 and KN-23. 1
Natural varianti71S → R in strain: KN-3, KN-9, KN-10 and L16. 1
Natural varianti121D → G in strain: TN256, 1420#1 and KO140. 1
Natural varianti121D → N in strain: KN-3, KN-9, KN-10 and L16. 1
Natural varianti138S → T in strain: JP-75, KN-7, KN-15, KN-17, KN-21 and KN-23. 1
Natural varianti144Y → H in strain: Canton-S. 1
Natural varianti156S → R in strain: 1420#1, KN-3, KN-9, KN-10, KO140, L16 and TN256. 1
Natural varianti181Y → N in strain: Canton-S. 1
Natural varianti231A → S in strain: JP-70, KN-17 and KN-21. 1
Natural varianti278D → N in strain: 1420#1, KO140, KN-3, KN-9, KN-10 and L16. 1
Natural varianti284T → I in strain: KN-21. 1
Natural varianti398T → A in strain: 1420#1, J87, JP-60, JP-70, JP-75, KO123, KO140, KN-9, KN-15, KN-21, L16 and TN256. 1
Natural varianti401S → L in strain: Berkeley. 1
Natural varianti403E → A in strain: J87, JP-60 and KO123. 1
Natural varianti465V → I in strain: 1420#1. 1
Natural varianti474S → Y in strain: KN-23. 1
Natural varianti476N → Y in strain: Berkeley, JP-1, JP-5, JP-15, JP-35, JP-55, JP-65, JP-84, KN-12, KN-22 and KN-27. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04569 Genomic DNA. Translation: CAA28238.1.
L22716 Genomic DNA. Translation: AAA92226.1.
L22719 Genomic DNA. Translation: AAA92229.1.
L22721 Genomic DNA. Translation: AAA92231.1.
L22725 Genomic DNA. Translation: AAA92235.1.
L22726 Genomic DNA. Translation: AAA92239.1.
L22729 Genomic DNA. Translation: AAA92240.1.
L22731 Genomic DNA. Translation: AAA92234.1.
L22733 Genomic DNA. Translation: AAA92241.1.
L22735 Genomic DNA. Translation: AAA92236.1.
AB042862 Genomic DNA. Translation: BAB32503.1.
AB042863 Genomic DNA. Translation: BAB32504.1.
AB042864 Genomic DNA. Translation: BAB32505.1.
AB042865 Genomic DNA. Translation: BAB32506.1.
AB042866 Genomic DNA. Translation: BAB32507.1.
AB042867 Genomic DNA. Translation: BAB32508.1.
AB042868 Genomic DNA. Translation: BAB32509.1.
AB042869 Genomic DNA. Translation: BAB32510.1.
AB042870 Genomic DNA. Translation: BAB32511.1.
AB042871 Genomic DNA. Translation: BAB32512.1.
AB042872 Genomic DNA. Translation: BAB32513.1.
AB042873 Genomic DNA. Translation: BAB32514.1.
AB042874 Genomic DNA. Translation: BAB32515.1.
AB042875 Genomic DNA. Translation: BAB32516.1.
AB042876 Genomic DNA. Translation: BAB32517.1.
AB042877 Genomic DNA. Translation: BAB32518.1.
AB042878 Genomic DNA. Translation: BAB32519.1.
AB042879 Genomic DNA. Translation: BAB32520.1.
AB042880 Genomic DNA. Translation: BAB32521.1.
AB042881 Genomic DNA. Translation: BAB32522.1.
AB042882 Genomic DNA. Translation: BAB32523.1.
AB042883 Genomic DNA. Translation: BAB32524.1.
AB042884 Genomic DNA. Translation: BAB72090.1.
AB042885 Genomic DNA. Translation: BAB72091.1.
AB042886 Genomic DNA. Translation: BAB72092.1.
AE013599 Genomic DNA. Translation: AAF57896.1.
AY051425 mRNA. Translation: AAK92849.1.
PIRiA25529.
S58953.
S58956.
S58957.
S58958.
S58961.
S58965.
RefSeqiNP_001286518.1. NM_001299589.1.
NP_536346.1. NM_080421.4.
UniGeneiDm.20319.

Genome annotation databases

GeneIDi47764.
KEGGidme:Dmel_CG18730.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAMYA_DROME
AccessioniPrimary (citable) accession number: P08144
Secondary accession number(s): Q27582
, Q27583, Q27584, Q27887, Q961R0, Q969D2, Q9BH42, Q9BH55, Q9BPT4, Q9BPT5, Q9BPT6, Q9BPT7, Q9BPT8, Q9BPT9, Q9BPU0, Q9BPU1, Q9V7Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: September 27, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families