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P08144

- AMYA_DROME

UniProt

P08144 - AMYA_DROME

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Protein

Alpha-amylase A

Gene
Amy-p, AmyA, CG18730
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Binds 1 calcium ion per subunit By similarity.
Binds 1 chloride ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161Calcium By similarity
Metal bindingi165 – 1651Calcium; via carbonyl oxygen By similarity
Metal bindingi174 – 1741Calcium By similarity
Binding sitei202 – 2021Chloride By similarity
Active sitei204 – 2041Nucleophile By similarity
Metal bindingi208 – 2081Calcium; via carbonyl oxygen By similarity
Active sitei241 – 2411Proton donor By similarity
Binding sitei304 – 3041Chloride By similarity
Sitei306 – 3061Transition state stabilizer By similarity
Binding sitei343 – 3431Chloride By similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB
  2. calcium ion binding Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase A (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-p
Synonyms:AmyA
ORF Names:CG18730
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000079. Amy-p.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Chaini19 – 494476Alpha-amylase APRO_0000001364Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acid By similarity
Disulfide bondi46 ↔ 102 By similarity
Disulfide bondi153 ↔ 167 By similarity
Disulfide bondi376 ↔ 382 By similarity
Disulfide bondi448 ↔ 460 By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP08144.
PRIDEiP08144.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

BioGridi70922. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP08144.
SMRiP08144. Positions 21-494.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
InParanoidiP08144.
KOiK01176.
OrthoDBiEOG7RJPR2.
PhylomeDBiP08144.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08144-1 [UniParc]FASTAAdd to Basket

« Hide

MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL    50
GPNGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEEQFASMVK 100
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPYSSLDFN 150
PTCAISNYND ANEVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG 200
FRVDAAKHMW PADLAVIYGR LKNLNTDHGF ASGSKAYIVQ EVIDMGGEAI 250
SKSEYTGLGA ITEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV 300
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF 350
TDTDQGPPTT DGHNIASPIF NSDNSCSGGW VCEHRWRQIY NMVAFRNTVG 400
SDEIQNWWDN GSNQISFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV 450
ISGSKSGSSC TGKTVTVGSD GRASINIGSS EDDGVLAIHV NAKL 494
Length:494
Mass (Da):53,746
Last modified:August 1, 1988 - v1
Checksum:iB477D3B44754C298
GO

Polymorphismi

At least 6 electrophoretic isozymes are known: Amy1, Amy2, Amy3, Amy4, Amy5 and Amy6. Strains J87 and KO123 express Amy2; KO140 and 1420#1 express Amy4; L16 expresses Amy5.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111A → S in strain: KN-28.
Natural varianti16 – 161A → V in strain: KN-22 and KN-23.
Natural varianti71 – 711S → R in strain: KN-3, KN-9, KN-10 and L16.
Natural varianti121 – 1211D → G in strain: TN256, 1420#1 and KO140.
Natural varianti121 – 1211D → N in strain: KN-3, KN-9, KN-10 and L16.
Natural varianti138 – 1381S → T in strain: JP-75, KN-7, KN-15, KN-17, KN-21 and KN-23.
Natural varianti144 – 1441Y → H in strain: Canton-S.
Natural varianti156 – 1561S → R in strain: 1420#1, KN-3, KN-9, KN-10, KO140, L16 and TN256.
Natural varianti181 – 1811Y → N in strain: Canton-S.
Natural varianti231 – 2311A → S in strain: JP-70, KN-17 and KN-21.
Natural varianti278 – 2781D → N in strain: 1420#1, KO140, KN-3, KN-9, KN-10 and L16.
Natural varianti284 – 2841T → I in strain: KN-21.
Natural varianti398 – 3981T → A in strain: 1420#1, J87, JP-60, JP-70, JP-75, KO123, KO140, KN-9, KN-15, KN-21, L16 and TN256.
Natural varianti401 – 4011S → L in strain: Berkeley.
Natural varianti403 – 4031E → A in strain: J87, JP-60 and KO123.
Natural varianti465 – 4651V → I in strain: 1420#1.
Natural varianti474 – 4741S → Y in strain: KN-23.
Natural varianti476 – 4761N → Y in strain: Berkeley, JP-1, JP-5, JP-15, JP-35, JP-55, JP-65, JP-84, KN-12, KN-22 and KN-27.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04569 Genomic DNA. Translation: CAA28238.1.
L22716 Genomic DNA. Translation: AAA92226.1.
L22719 Genomic DNA. Translation: AAA92229.1.
L22721 Genomic DNA. Translation: AAA92231.1.
L22725 Genomic DNA. Translation: AAA92235.1.
L22726 Genomic DNA. Translation: AAA92239.1.
L22729 Genomic DNA. Translation: AAA92240.1.
L22731 Genomic DNA. Translation: AAA92234.1.
L22733 Genomic DNA. Translation: AAA92241.1.
L22735 Genomic DNA. Translation: AAA92236.1.
AB042862 Genomic DNA. Translation: BAB32503.1.
AB042863 Genomic DNA. Translation: BAB32504.1.
AB042864 Genomic DNA. Translation: BAB32505.1.
AB042865 Genomic DNA. Translation: BAB32506.1.
AB042866 Genomic DNA. Translation: BAB32507.1.
AB042867 Genomic DNA. Translation: BAB32508.1.
AB042868 Genomic DNA. Translation: BAB32509.1.
AB042869 Genomic DNA. Translation: BAB32510.1.
AB042870 Genomic DNA. Translation: BAB32511.1.
AB042871 Genomic DNA. Translation: BAB32512.1.
AB042872 Genomic DNA. Translation: BAB32513.1.
AB042873 Genomic DNA. Translation: BAB32514.1.
AB042874 Genomic DNA. Translation: BAB32515.1.
AB042875 Genomic DNA. Translation: BAB32516.1.
AB042876 Genomic DNA. Translation: BAB32517.1.
AB042877 Genomic DNA. Translation: BAB32518.1.
AB042878 Genomic DNA. Translation: BAB32519.1.
AB042879 Genomic DNA. Translation: BAB32520.1.
AB042880 Genomic DNA. Translation: BAB32521.1.
AB042881 Genomic DNA. Translation: BAB32522.1.
AB042882 Genomic DNA. Translation: BAB32523.1.
AB042883 Genomic DNA. Translation: BAB32524.1.
AB042884 Genomic DNA. Translation: BAB72090.1.
AB042885 Genomic DNA. Translation: BAB72091.1.
AB042886 Genomic DNA. Translation: BAB72092.1.
AE013599 Genomic DNA. Translation: AAF57896.1.
AY051425 mRNA. Translation: AAK92849.1.
PIRiA25529.
S58953.
S58956.
S58957.
S58958.
S58961.
S58965.
RefSeqiNP_536346.1. NM_080421.3.
UniGeneiDm.20319.

Genome annotation databases

GeneIDi47764.
KEGGidme:Dmel_CG18730.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04569 Genomic DNA. Translation: CAA28238.1 .
L22716 Genomic DNA. Translation: AAA92226.1 .
L22719 Genomic DNA. Translation: AAA92229.1 .
L22721 Genomic DNA. Translation: AAA92231.1 .
L22725 Genomic DNA. Translation: AAA92235.1 .
L22726 Genomic DNA. Translation: AAA92239.1 .
L22729 Genomic DNA. Translation: AAA92240.1 .
L22731 Genomic DNA. Translation: AAA92234.1 .
L22733 Genomic DNA. Translation: AAA92241.1 .
L22735 Genomic DNA. Translation: AAA92236.1 .
AB042862 Genomic DNA. Translation: BAB32503.1 .
AB042863 Genomic DNA. Translation: BAB32504.1 .
AB042864 Genomic DNA. Translation: BAB32505.1 .
AB042865 Genomic DNA. Translation: BAB32506.1 .
AB042866 Genomic DNA. Translation: BAB32507.1 .
AB042867 Genomic DNA. Translation: BAB32508.1 .
AB042868 Genomic DNA. Translation: BAB32509.1 .
AB042869 Genomic DNA. Translation: BAB32510.1 .
AB042870 Genomic DNA. Translation: BAB32511.1 .
AB042871 Genomic DNA. Translation: BAB32512.1 .
AB042872 Genomic DNA. Translation: BAB32513.1 .
AB042873 Genomic DNA. Translation: BAB32514.1 .
AB042874 Genomic DNA. Translation: BAB32515.1 .
AB042875 Genomic DNA. Translation: BAB32516.1 .
AB042876 Genomic DNA. Translation: BAB32517.1 .
AB042877 Genomic DNA. Translation: BAB32518.1 .
AB042878 Genomic DNA. Translation: BAB32519.1 .
AB042879 Genomic DNA. Translation: BAB32520.1 .
AB042880 Genomic DNA. Translation: BAB32521.1 .
AB042881 Genomic DNA. Translation: BAB32522.1 .
AB042882 Genomic DNA. Translation: BAB32523.1 .
AB042883 Genomic DNA. Translation: BAB32524.1 .
AB042884 Genomic DNA. Translation: BAB72090.1 .
AB042885 Genomic DNA. Translation: BAB72091.1 .
AB042886 Genomic DNA. Translation: BAB72092.1 .
AE013599 Genomic DNA. Translation: AAF57896.1 .
AY051425 mRNA. Translation: AAK92849.1 .
PIRi A25529.
S58953.
S58956.
S58957.
S58958.
S58961.
S58965.
RefSeqi NP_536346.1. NM_080421.3.
UniGenei Dm.20319.

3D structure databases

ProteinModelPortali P08144.
SMRi P08144. Positions 21-494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 70922. 2 interactions.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbi P08144.
PRIDEi P08144.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 47764.
KEGGi dme:Dmel_CG18730.

Organism-specific databases

CTDi 47764.
FlyBasei FBgn0000079. Amy-p.

Phylogenomic databases

eggNOGi COG0366.
InParanoidi P08144.
KOi K01176.
OrthoDBi EOG7RJPR2.
PhylomeDBi P08144.

Miscellaneous databases

GenomeRNAii 47764.
NextBioi 839119.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The alpha-amylase gene in Drosophila melanogaster: nucleotide sequence, gene structure and expression motifs."
    Boer P.H., Hickey D.A.
    Nucleic Acids Res. 14:8399-8411(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Canton-S and Oregon-R.
  2. "Evolutionary relationships and sequence variation of alpha-amylase variants encoded by duplicated genes in the Amy locus of Drosophila melanogaster."
    Inomata N., Shibata H., Okuyama E., Yamazaki T.
    Genetics 141:237-244(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 1420#1, AO168, J87, KO123, KO140, L16, TN22, TN256 and TN329.
  3. "Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster: evidence of positive selection in the coding regions and selective constraints in the cis-regulatory regions."
    Araki H., Inomata N., Yamazaki T.
    Genetics 157:667-677(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS.
    Strain: JP-1, JP-15, JP-169, JP-186, JP-190, JP-35, JP-5, JP-55, JP-60, JP-65, JP-70, JP-75, JP-84, KN-10, KN-12, KN-15, KN-17, KN-21, KN-22, KN-23, KN-27, KN-28, KN-3, KN-7 and KN-9.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiAMYA_DROME
AccessioniPrimary (citable) accession number: P08144
Secondary accession number(s): Q27582
, Q27583, Q27584, Q27887, Q961R0, Q969D2, Q9BH42, Q9BH55, Q9BPT4, Q9BPT5, Q9BPT6, Q9BPT7, Q9BPT8, Q9BPT9, Q9BPU0, Q9BPU1, Q9V7Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi