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P08144

- AMYA_DROME

UniProt

P08144 - AMYA_DROME

Protein

Alpha-amylase A

Gene

Amy-p

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity
    Binds 1 chloride ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi116 – 1161CalciumBy similarity
    Metal bindingi165 – 1651Calcium; via carbonyl oxygenBy similarity
    Metal bindingi174 – 1741CalciumBy similarity
    Binding sitei202 – 2021ChlorideBy similarity
    Active sitei204 – 2041NucleophileBy similarity
    Metal bindingi208 – 2081Calcium; via carbonyl oxygenBy similarity
    Active sitei241 – 2411Proton donorBy similarity
    Binding sitei304 – 3041ChlorideBy similarity
    Sitei306 – 3061Transition state stabilizerBy similarity
    Binding sitei343 – 3431ChlorideBy similarity

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB
    2. calcium ion binding Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Chloride, Metal-binding

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylase A (EC:3.2.1.1)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Gene namesi
    Name:Amy-p
    Synonyms:AmyA
    ORF Names:CG18730
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0000079. Amy-p.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 494476Alpha-amylase APRO_0000001364Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Pyrrolidone carboxylic acidBy similarity
    Disulfide bondi46 ↔ 102By similarity
    Disulfide bondi153 ↔ 167By similarity
    Disulfide bondi376 ↔ 382By similarity
    Disulfide bondi448 ↔ 460By similarity

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP08144.
    PRIDEiP08144.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi70922. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP08144.
    SMRiP08144. Positions 21-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0366.
    InParanoidiP08144.
    KOiK01176.
    OrthoDBiEOG7RJPR2.
    PhylomeDBiP08144.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08144-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL    50
    GPNGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEEQFASMVK 100
    RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPYSSLDFN 150
    PTCAISNYND ANEVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG 200
    FRVDAAKHMW PADLAVIYGR LKNLNTDHGF ASGSKAYIVQ EVIDMGGEAI 250
    SKSEYTGLGA ITEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV 300
    FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF 350
    TDTDQGPPTT DGHNIASPIF NSDNSCSGGW VCEHRWRQIY NMVAFRNTVG 400
    SDEIQNWWDN GSNQISFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV 450
    ISGSKSGSSC TGKTVTVGSD GRASINIGSS EDDGVLAIHV NAKL 494
    Length:494
    Mass (Da):53,746
    Last modified:August 1, 1988 - v1
    Checksum:iB477D3B44754C298
    GO

    Polymorphismi

    At least 6 electrophoretic isozymes are known: Amy1, Amy2, Amy3, Amy4, Amy5 and Amy6. Strains J87 and KO123 express Amy2; KO140 and 1420#1 express Amy4; L16 expresses Amy5.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti11 – 111A → S in strain: KN-28.
    Natural varianti16 – 161A → V in strain: KN-22 and KN-23.
    Natural varianti71 – 711S → R in strain: KN-3, KN-9, KN-10 and L16.
    Natural varianti121 – 1211D → G in strain: TN256, 1420#1 and KO140.
    Natural varianti121 – 1211D → N in strain: KN-3, KN-9, KN-10 and L16.
    Natural varianti138 – 1381S → T in strain: JP-75, KN-7, KN-15, KN-17, KN-21 and KN-23.
    Natural varianti144 – 1441Y → H in strain: Canton-S.
    Natural varianti156 – 1561S → R in strain: 1420#1, KN-3, KN-9, KN-10, KO140, L16 and TN256.
    Natural varianti181 – 1811Y → N in strain: Canton-S.
    Natural varianti231 – 2311A → S in strain: JP-70, KN-17 and KN-21.
    Natural varianti278 – 2781D → N in strain: 1420#1, KO140, KN-3, KN-9, KN-10 and L16.
    Natural varianti284 – 2841T → I in strain: KN-21.
    Natural varianti398 – 3981T → A in strain: 1420#1, J87, JP-60, JP-70, JP-75, KO123, KO140, KN-9, KN-15, KN-21, L16 and TN256.
    Natural varianti401 – 4011S → L in strain: Berkeley.
    Natural varianti403 – 4031E → A in strain: J87, JP-60 and KO123.
    Natural varianti465 – 4651V → I in strain: 1420#1.
    Natural varianti474 – 4741S → Y in strain: KN-23.
    Natural varianti476 – 4761N → Y in strain: Berkeley, JP-1, JP-5, JP-15, JP-35, JP-55, JP-65, JP-84, KN-12, KN-22 and KN-27.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04569 Genomic DNA. Translation: CAA28238.1.
    L22716 Genomic DNA. Translation: AAA92226.1.
    L22719 Genomic DNA. Translation: AAA92229.1.
    L22721 Genomic DNA. Translation: AAA92231.1.
    L22725 Genomic DNA. Translation: AAA92235.1.
    L22726 Genomic DNA. Translation: AAA92239.1.
    L22729 Genomic DNA. Translation: AAA92240.1.
    L22731 Genomic DNA. Translation: AAA92234.1.
    L22733 Genomic DNA. Translation: AAA92241.1.
    L22735 Genomic DNA. Translation: AAA92236.1.
    AB042862 Genomic DNA. Translation: BAB32503.1.
    AB042863 Genomic DNA. Translation: BAB32504.1.
    AB042864 Genomic DNA. Translation: BAB32505.1.
    AB042865 Genomic DNA. Translation: BAB32506.1.
    AB042866 Genomic DNA. Translation: BAB32507.1.
    AB042867 Genomic DNA. Translation: BAB32508.1.
    AB042868 Genomic DNA. Translation: BAB32509.1.
    AB042869 Genomic DNA. Translation: BAB32510.1.
    AB042870 Genomic DNA. Translation: BAB32511.1.
    AB042871 Genomic DNA. Translation: BAB32512.1.
    AB042872 Genomic DNA. Translation: BAB32513.1.
    AB042873 Genomic DNA. Translation: BAB32514.1.
    AB042874 Genomic DNA. Translation: BAB32515.1.
    AB042875 Genomic DNA. Translation: BAB32516.1.
    AB042876 Genomic DNA. Translation: BAB32517.1.
    AB042877 Genomic DNA. Translation: BAB32518.1.
    AB042878 Genomic DNA. Translation: BAB32519.1.
    AB042879 Genomic DNA. Translation: BAB32520.1.
    AB042880 Genomic DNA. Translation: BAB32521.1.
    AB042881 Genomic DNA. Translation: BAB32522.1.
    AB042882 Genomic DNA. Translation: BAB32523.1.
    AB042883 Genomic DNA. Translation: BAB32524.1.
    AB042884 Genomic DNA. Translation: BAB72090.1.
    AB042885 Genomic DNA. Translation: BAB72091.1.
    AB042886 Genomic DNA. Translation: BAB72092.1.
    AE013599 Genomic DNA. Translation: AAF57896.1.
    AY051425 mRNA. Translation: AAK92849.1.
    PIRiA25529.
    S58953.
    S58956.
    S58957.
    S58958.
    S58961.
    S58965.
    RefSeqiNP_536346.1. NM_080421.3.
    UniGeneiDm.20319.

    Genome annotation databases

    GeneIDi47764.
    KEGGidme:Dmel_CG18730.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04569 Genomic DNA. Translation: CAA28238.1 .
    L22716 Genomic DNA. Translation: AAA92226.1 .
    L22719 Genomic DNA. Translation: AAA92229.1 .
    L22721 Genomic DNA. Translation: AAA92231.1 .
    L22725 Genomic DNA. Translation: AAA92235.1 .
    L22726 Genomic DNA. Translation: AAA92239.1 .
    L22729 Genomic DNA. Translation: AAA92240.1 .
    L22731 Genomic DNA. Translation: AAA92234.1 .
    L22733 Genomic DNA. Translation: AAA92241.1 .
    L22735 Genomic DNA. Translation: AAA92236.1 .
    AB042862 Genomic DNA. Translation: BAB32503.1 .
    AB042863 Genomic DNA. Translation: BAB32504.1 .
    AB042864 Genomic DNA. Translation: BAB32505.1 .
    AB042865 Genomic DNA. Translation: BAB32506.1 .
    AB042866 Genomic DNA. Translation: BAB32507.1 .
    AB042867 Genomic DNA. Translation: BAB32508.1 .
    AB042868 Genomic DNA. Translation: BAB32509.1 .
    AB042869 Genomic DNA. Translation: BAB32510.1 .
    AB042870 Genomic DNA. Translation: BAB32511.1 .
    AB042871 Genomic DNA. Translation: BAB32512.1 .
    AB042872 Genomic DNA. Translation: BAB32513.1 .
    AB042873 Genomic DNA. Translation: BAB32514.1 .
    AB042874 Genomic DNA. Translation: BAB32515.1 .
    AB042875 Genomic DNA. Translation: BAB32516.1 .
    AB042876 Genomic DNA. Translation: BAB32517.1 .
    AB042877 Genomic DNA. Translation: BAB32518.1 .
    AB042878 Genomic DNA. Translation: BAB32519.1 .
    AB042879 Genomic DNA. Translation: BAB32520.1 .
    AB042880 Genomic DNA. Translation: BAB32521.1 .
    AB042881 Genomic DNA. Translation: BAB32522.1 .
    AB042882 Genomic DNA. Translation: BAB32523.1 .
    AB042883 Genomic DNA. Translation: BAB32524.1 .
    AB042884 Genomic DNA. Translation: BAB72090.1 .
    AB042885 Genomic DNA. Translation: BAB72091.1 .
    AB042886 Genomic DNA. Translation: BAB72092.1 .
    AE013599 Genomic DNA. Translation: AAF57896.1 .
    AY051425 mRNA. Translation: AAK92849.1 .
    PIRi A25529.
    S58953.
    S58956.
    S58957.
    S58958.
    S58961.
    S58965.
    RefSeqi NP_536346.1. NM_080421.3.
    UniGenei Dm.20319.

    3D structure databases

    ProteinModelPortali P08144.
    SMRi P08144. Positions 21-494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 70922. 2 interactions.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi P08144.
    PRIDEi P08144.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 47764.
    KEGGi dme:Dmel_CG18730.

    Organism-specific databases

    CTDi 47764.
    FlyBasei FBgn0000079. Amy-p.

    Phylogenomic databases

    eggNOGi COG0366.
    InParanoidi P08144.
    KOi K01176.
    OrthoDBi EOG7RJPR2.
    PhylomeDBi P08144.

    Miscellaneous databases

    GenomeRNAii 47764.
    NextBioi 839119.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The alpha-amylase gene in Drosophila melanogaster: nucleotide sequence, gene structure and expression motifs."
      Boer P.H., Hickey D.A.
      Nucleic Acids Res. 14:8399-8411(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Canton-S and Oregon-R.
    2. "Evolutionary relationships and sequence variation of alpha-amylase variants encoded by duplicated genes in the Amy locus of Drosophila melanogaster."
      Inomata N., Shibata H., Okuyama E., Yamazaki T.
      Genetics 141:237-244(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1420#1, AO168, J87, KO123, KO140, L16, TN22, TN256 and TN329.
    3. "Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster: evidence of positive selection in the coding regions and selective constraints in the cis-regulatory regions."
      Araki H., Inomata N., Yamazaki T.
      Genetics 157:667-677(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS.
      Strain: JP-1, JP-15, JP-169, JP-186, JP-190, JP-35, JP-5, JP-55, JP-60, JP-65, JP-70, JP-75, JP-84, KN-10, KN-12, KN-15, KN-17, KN-21, KN-22, KN-23, KN-27, KN-28, KN-3, KN-7 and KN-9.
    4. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    5. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Head.

    Entry informationi

    Entry nameiAMYA_DROME
    AccessioniPrimary (citable) accession number: P08144
    Secondary accession number(s): Q27582
    , Q27583, Q27584, Q27887, Q961R0, Q969D2, Q9BH42, Q9BH55, Q9BPT4, Q9BPT5, Q9BPT6, Q9BPT7, Q9BPT8, Q9BPT9, Q9BPU0, Q9BPU1, Q9V7Y8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3