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Protein

Lantibiotic epidermin

Gene

epiA

Organism
Staphylococcus epidermidis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Lantibiotic

Protein family/group databases

TCDBi1.C.20.1.6. the nisin (nisin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lantibiotic epidermin
Gene namesi
Name:epiA
Encoded oniPlasmid pTu 320 Publication
OrganismiStaphylococcus epidermidis
Taxonomic identifieri1282 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000171161 – 301 PublicationAdd BLAST30
PeptideiPRO_000001711731 – 52Lantibiotic epidermin1 PublicationAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki33 ↔ 37Lanthionine (Ser-Cys)
Cross-linki38 ↔ 41Beta-methyllanthionine (Thr-Cys)
Modified residuei44(Z)-2,3-didehydrobutyrine1
Cross-linki46 ↔ 51Lanthionine (Ser-Cys)
Cross-linki49 ↔ 52S-(2-aminovinyl)-D-cysteine (Ser-Cys)

Post-translational modificationi

Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The C-terminal lanthionine undergoes decarboxylation. This is followed by membrane translocation and cleavage of the modified precursor.
The 2,3-didehydrobutyrine is determined to be the Z-isomer.1 Publication

Keywords - PTMi

D-amino acid, Thioether bond

Miscellaneous databases

PMAP-CutDBP08136.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G5QX-ray2.57M/N/O/P48-52[»]
SMRiP08136.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08136.

Family & Domainsi

Sequence similaritiesi

Belongs to the type A lantibiotic family.Curated

Family and domain databases

InterProiIPR006079. Lantibiotic_typ-A_Bacillales.
[Graphical view]
PfamiPF02052. Gallidermin. 1 hit.
[Graphical view]
PRINTSiPR00323. GALLIDERMIN.
TIGRFAMsiTIGR03731. lantibio_gallid. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVKEKNDL FNLDVKVNAK ESNDSGAEPR IASKFICTPG CAKTGSFNSY

CC
Length:52
Mass (Da):5,632
Last modified:August 1, 1988 - v1
Checksum:i8B1AD2875BF16D6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07840 Genomic DNA. Translation: CAA30689.1.
X07840 Genomic DNA. Translation: CAA30690.1.
X62386 Genomic DNA. Translation: CAA44252.1.
PIRiS00768. EPSED.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07840 Genomic DNA. Translation: CAA30689.1.
X07840 Genomic DNA. Translation: CAA30690.1.
X62386 Genomic DNA. Translation: CAA44252.1.
PIRiS00768. EPSED.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G5QX-ray2.57M/N/O/P48-52[»]
SMRiP08136.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.20.1.6. the nisin (nisin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08136.
PMAP-CutDBP08136.

Family and domain databases

InterProiIPR006079. Lantibiotic_typ-A_Bacillales.
[Graphical view]
PfamiPF02052. Gallidermin. 1 hit.
[Graphical view]
PRINTSiPR00323. GALLIDERMIN.
TIGRFAMsiTIGR03731. lantibio_gallid. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLANE_STAEP
AccessioniPrimary (citable) accession number: P08136
Secondary accession number(s): Q54093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.