ID   NAHH_PSEPU              Reviewed;         307 AA.
AC   P08127;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   16-JUN-2009, entry version 71.
DE   RecName: Full=Metapyrocatechase;
DE            Short=MPC;
DE            EC=1.13.11.2;
DE   AltName: Full=CatO2ase;
DE   AltName: Full=Catechol 2,3-dioxygenase;
GN   Name=nahH;
OS   Pseudomonas putida.
OG   Plasmid NAH7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17485 / DSM 50208 / Stanier 111 / JCM 6158 / Biotype A;
RX   MEDLINE=88142541; PubMed=3481421; DOI=10.1007/BF00325689;
RA   Harayama S., Rekik M., Wasserfallen A., Bairoch A.;
RT   "Evolutionary relationships between catabolic pathways for aromatics:
RT   conservation of gene order and nucleotide sequences of catechol
RT   oxidation genes of pWW0 and NAH7 plasmids.";
RL   Mol. Gen. Genet. 210:241-247(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17485 / DSM 50208 / Stanier 111 / JCM 6158 / Biotype A;
RX   MEDLINE=87305588; PubMed=3623105; DOI=10.1016/0378-1119(87)90244-7;
RA   Ghosal D., You I.-S., Gunsalus I.C.;
RT   "Nucleotide sequence and expression of gene nahH of plasmid NAH7 and
RT   homology with gene xylE of TOL pWWO.";
RL   Gene 55:19-28(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
RC   STRAIN=ATCC 17485 / DSM 50208 / Stanier 111 / JCM 6158 / Biotype A;
RX   MEDLINE=91129237; PubMed=1993181; DOI=10.1021/bi00220a028;
RA   You I.-S., Ghosal D., Gunsalus I.C.;
RT   "Nucleotide sequence analysis of the Pseudomonas putida PpG7
RT   salicylate hydroxylase gene (nahG) and its 3'-flanking region.";
RL   Biochemistry 30:1635-1641(1991).
CC   -!- CATALYTIC ACTIVITY: Catechol + O(2) = 2-hydroxymuconate
CC       semialdehyde.
CC   -!- COFACTOR: Fe(2+) ion.
CC   -!- PATHWAY: Aromatic compound metabolism; benzoic acid degradation
CC       via hydroxylation.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the extradiol ring-cleavage dioxygenase
CC       family.
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DR   EMBL; M17159; AAA98183.1; -; Genomic_DNA.
DR   EMBL; X06412; CAA29701.1; -; Genomic_DNA.
DR   EMBL; M60055; AAA25899.1; -; Genomic_DNA.
DR   PIR; A27389; A27389.
DR   RefSeq; YP_534833.1; -.
DR   HSSP; P06622; 1MPY.
DR   SMR; P08127; 1-307.
DR   GeneID; 3974217; -.
DR   BRENDA; 1.13.11.2; 403.
DR   GO; GO:0018577; F:catechol 2,3-dioxygenase activity; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017624; Catechol_2-3_dOase.
DR   InterPro; IPR004360; Glyas_bleo-R_dOase.
DR   InterPro; IPR000486; Xdiol_dOase_1_2.
DR   Pfam; PF00903; Glyoxalase; 2.
DR   ProDom; PD002334; Gly_diox; 2.
DR   TIGRFAMs; TIGR03211; catechol_2_3; 1.
DR   PROSITE; PS00082; EXTRADIOL_DIOXYGENAS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Metal-binding;
KW   Oxidoreductase; Plasmid.
FT   CHAIN         1    307       Metapyrocatechase.
FT                                /FTId=PRO_0000085024.
FT   METAL       153    153       Iron (By similarity).
FT   METAL       214    214       Iron (By similarity).
FT   METAL       265    265       Iron (By similarity).
SQ   SEQUENCE   307 AA;  34913 MW;  140465CAA0CE7F4E CRC64;
     MNKGVMRPGH VQLRVLDMGK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV DKFSVVLREA
     DEPGMDFMGF KVVDEDSLNR LTDDLLNFGC LIENVAAGEL KGCGRRVRFQ APSGHHFELY
     ADKEYTGKWG VSEVNPEAWP RDLKGMAAVR FDHCLLYGDE LQATYELFTE VLGFYLAEQV
     VDADGIRLAQ FLSLSTKAHD VAFIHHAEKG KFHHASFFLD TWEDVLRAAD LISMTDTSID
     IGPTRHGLTQ GKTIYFFDPS GNRCEVFCGG NYNYPDHKPV TWLAKDVGKA IFYHDRVLNE
     RFMTVMT
//