Reviewed, UniProtKB/Swiss-Prot P08127 (NAHH_PSEPU)

Names and origin

Protein names

Recommended name:
    Metapyrocatechase
      Short name=MPC
    EC=1.13.11.2
Alternative name(s):
    CatO2ase
    Catechol 2,3-dioxygenase

Gene names

Name:

nahH

Encoded on

Plasmid NAH7

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Catechol + O2 = 2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; benzoic acid degradation via hydroxylation.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Metapyrocatechase		PRO_0000085024
Sites
	Metal binding	153	1	Iron By similarity
	Metal binding	214	1	Iron By similarity
	Metal binding	265	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08127-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 140465CAA0CE7F4E	FASTA	307	34,913
	10 20 30 40 50 60 MNKGVMRPGH VQLRVLDMGK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV DKFSVVLREA 70 80 90 100 110 120 DEPGMDFMGF KVVDEDSLNR LTDDLLNFGC LIENVAAGEL KGCGRRVRFQ APSGHHFELY 130 140 150 160 170 180 ADKEYTGKWG VSEVNPEAWP RDLKGMAAVR FDHCLLYGDE LQATYELFTE VLGFYLAEQV 190 200 210 220 230 240 VDADGIRLAQ FLSLSTKAHD VAFIHHAEKG KFHHASFFLD TWEDVLRAAD LISMTDTSID 250 260 270 280 290 300 IGPTRHGLTQ GKTIYFFDPS GNRCEVFCGG NYNYPDHKPV TWLAKDVGKA IFYHDRVLNE RFMTVMT

References

[1]	"Evolutionary relationships between catabolic pathways for aromatics: conservation of gene order and nucleotide sequences of catechol oxidation genes of pWW0 and NAH7 plasmids." Harayama S., Rekik M., Wasserfallen A., Bairoch A. Mol. Gen. Genet. 210:241-247(1987) [PubMed: 3481421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17485 / DSM 50208 / Stanier 111 / JCM 6158 / Biotype A.
[2]	"Nucleotide sequence and expression of gene nahH of plasmid NAH7 and homology with gene xylE of TOL pWWO." Ghosal D., You I.-S., Gunsalus I.C. Gene 55:19-28(1987) [PubMed: 3623105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17485 / DSM 50208 / Stanier 111 / JCM 6158 / Biotype A.
[3]	"Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region." You I.-S., Ghosal D., Gunsalus I.C. Biochemistry 30:1635-1641(1991) [PubMed: 1993181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8. Strain: ATCC 17485 / DSM 50208 / Stanier 111 / JCM 6158 / Biotype A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17159 Genomic DNA. Translation: AAA98183.1. X06412 Genomic DNA. Translation: CAA29701.1. M60055 Genomic DNA. Translation: AAA25899.1.
PIR	A27389.
RefSeq	YP_534833.1.
3D structure databases
HSSP	HSSP built from PDB template 1MPY based on UniProtKB P06622.
SMR	P08127. Positions 1-307.
ModBase	Search...
Genome annotation databases
GeneID	3974217.
Enzyme and pathway databases
BRENDA	1.13.11.2. 403.
Family and domain databases
InterPro	IPR017624. Catechol_2-3_dOase. IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_dOase_1_2. [Graphical view]
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
ProDom	PD002334. Gly_diox. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03211. catechol_2_3. 1 hit.
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAHH_PSEPU

Accession

Primary (citable) accession number: P08127

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)