P08121 (CO3A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Collagen alpha-1(III) chain

Gene names

Name:

Col3a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Collagen type III occurs in most soft connective tissues along with type I collagen.
Subunit structure	Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Domain	The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function By similarity.
Post-translational modification	Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group By similarity.
Sequence similarities	Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Collagen Repeat Signal
Ligand	Calcium Metal-binding
PTM	Disulfide bond Glycoprotein Hydroxylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood vessel development Inferred from mutant phenotype PubMed 9050868. Source: MGI cell-matrix adhesion Inferred from electronic annotation. Source: Compara cellular response to amino acid stimulus Inferred from direct assay PubMed 20548288. Source: MGI collagen biosynthetic process Inferred from electronic annotation. Source: Compara collagen fibril organization Inferred from mutant phenotype PubMed 9050868. Source: MGI digestive tract development Inferred from mutant phenotype PubMed 9050868. Source: MGI extracellular fibril organization Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara integrin-mediated signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of immune response Inferred from electronic annotation. Source: Compara peptide cross-linking Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara response to radiation Inferred from electronic annotation. Source: Compara skeletal system development Inferred from electronic annotation. Source: Compara skin development Inferred from electronic annotation. Source: Compara transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	collagen type III Inferred from direct assay PubMed 7825727. Source: MGI extracellular space Inferred from electronic annotation. Source: Compara
Molecular_function	extracellular matrix structural constituent Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Propeptide	24 – 154	131	N-terminal propeptide		PRO_0000005743
	Chain	155 – 1219	1065	Collagen alpha-1(III) chain		PRO_0000005744
	Propeptide	1220 – 1464	245	C-terminal propeptide		PRO_0000005745
Regions
	Domain	31 – 90	60	VWFC
	Domain	1230 – 1464	235	Fibrillar collagen NC1
	Region	155 – 169	15	Nonhelical region (N-terminal)
	Region	170 – 1195	1026	Triple-helical region
Sites
	Metal binding	1278	1	Calcium By similarity
	Metal binding	1280	1	Calcium By similarity
	Metal binding	1281	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	1283	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	1286	1	Calcium By similarity
Amino acid modifications
	Modified residue	262	1	5-hydroxylysine; alternate By similarity
	Modified residue	283	1	5-hydroxylysine By similarity
	Modified residue	859	1	5-hydroxylysine By similarity
	Modified residue	976	1	5-hydroxylysine By similarity
	Modified residue	1093	1	5-hydroxylysine By similarity
	Modified residue	1105	1	5-hydroxylysine By similarity
	Glycosylation	262	1	O-linked (Gal...); alternate By similarity
	Disulfide bond	1195		Interchain By similarity
	Disulfide bond	1196		Interchain By similarity
	Disulfide bond	1260 ↔ 1292		By similarity
	Disulfide bond	1266		Interchain (with C-1283) By similarity
	Disulfide bond	1283		Interchain (with C-1266) By similarity
	Disulfide bond	1300 ↔ 1462		By similarity
	Disulfide bond	1370 ↔ 1415		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08121 [UniParc]. Last modified July 15, 1999. Version 4. Checksum: 2104EC27A886090B	FASTA	1,464	138,943
	10 20 30 40 50 60 MMSFVQSGTW FLLTLLHPTL ILAQQSNVDE LGCSHLGQSY ESRDVWKPEP CQICVCDSGS 70 80 90 100 110 120 VLCDDIICDE EPLDCPNPEI PFGECCAICP QPSTPAPVLP DGHGPQGPKG DPGPPGIPGR 130 140 150 160 170 180 NGDPGLPGQP GLPGPPGSPG ICESCPTGGQ NYSPQFDSYD VKSGVGGMGG YPGPAGPPGP 190 200 210 220 230 240 PGPPGSSGHP GSPGSPGYQG PPGEPGQAGP AGPPGPPGAL GPAGPAGKDG ESGRPGRPGE 250 260 270 280 290 300 RGLPGPPGIK GPAGMPGFPG MKGHRGFDGR NGEKGETGAP GLKGENGLPG DNGAPGPMGP 310 320 330 340 350 360 RGAPGERGRP GLPGAAGARG NDGARGSDGQ PGPPGPPGTA GFPGSPGAKG EVGPAGSPGS 370 380 390 400 410 420 NGSPGQRGEP GPQGHAGAQG PPGPPGNNGS PGGKGEMGPA GIPGAPGLIG ARGPPGPAGT 430 440 450 460 470 480 NGIPGTRGPS GEPGKNGAKG EPGARGERGE AGSPGIPGPK GEDGKDGSPG EPGANGLPGA 490 500 510 520 530 540 AGERGPSGFR GPAGPNGIPG EKGPPGERGG PGPAGPRGVA GEPGRDGTPG GPGIRGMPGS 550 560 570 580 590 600 PGGPGNDGKP GPPGSQGESG RPGPPGPSGP RGQPGVMGFP GPKGNDGAPG KNGERGGPGG 610 620 630 640 650 660 PGLPGPAGKN GETGPQGPPG PTGPAGDKGD SGPPGPQGLQ GIPGTGGPPG ENGKPGEPGP 670 680 690 700 710 720 KGEVGAPGAP GGKGDSGAPG ERGPPGTAGI PGARGGAGPP GPEGGKGPAG PPGPPGASGS 730 740 750 760 770 780 PGLQGMPGER GGPGSPGPKG EKGEPGGAGA DGVPGKDGPR GPAGPIGPPG PAGQPGDKGE 790 800 810 820 830 840 GGSPGLPGIA GPRGGPGERG EHGPPGPAGF PGAPGQNGEP GAKGERGAPG EKGEGGPPGP 850 860 870 880 890 900 AGPTGSSGPA GPPGPQGVKG ERGSPGGPGT AGFPGGRGLP GPPGNNGNPG PPGPSGAPGK 910 920 930 940 950 960 DGPPGPAGNS GSPGNPGIAG PKGDAGQPGE KGPPGAQGPP GSPGPLGIAG LTGARGLAGP 970 980 990 1000 1010 1020 PGMPGPRGSP GPQGIKGESG KPGASGHNGE RGPPGPQGLP GQPGTAGEPG RDGNPGSDGQ 1030 1040 1050 1060 1070 1080 PGRDGSPGGK GDRGENGSPG APGAPGHPGP PGPVGPSGKS GDRGETGPAG PSGAPGPAGA 1090 1100 1110 1120 1130 1140 RGAPGPQGPR GDKGETGERG SNGIKGHRGF PGNPGPPGSP GAAGHQGAIG SPGPAGPRGP 1150 1160 1170 1180 1190 1200 VGPHGPPGKD GTSGHPGPIG PPGPRGNRGE RGSEGSPGHP GQPGPPGPPG APGPCCGGGA 1210 1220 1230 1240 1250 1260 AAIAGVGGEK SGGFSPYYGD DPMDFKINTE EIMSSLKSVN GQIESLISPD GSRKNPARNC 1270 1280 1290 1300 1310 1320 RDLKFCHPEL KSGEYWVDPN QGCKMDAIKV FCNMETGETC INASPMTVPR KHWWTDSGAE 1330 1340 1350 1360 1370 1380 KKHVWFGESM NGGFQFSYGP PDLPEDVVDV QLAFLRLLSS RASQNITYHC KNSIAYMDQA 1390 1400 1410 1420 1430 1440 SGNVKKSLKL MGSNEGEFKA EGNSKFTYTV LEDGCTKHTG EWSKTVFEYQ TRKAMRLPII 1450 1460 DIAPYDIGGP DQEFGVDIGP VCFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse type-III procollagen-encoding gene: genomic cloning and complete DNA sequence." Toman D., de Crombrugghe B. Gene 147:161-168(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X DBA. Tissue: Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Complete nucleotide sequence of the N-terminal domains of the murine alpha-1 type-III collagen chain." Wood L., Theriault N., Vogeli G. Gene 61:225-230(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-488.
[4]	"Identification of the promoter and first exon of the mouse alpha 1 (III) collagen gene." Liau G., Mudryj M., de Crombrugghe B. J. Biol. Chem. 260:3773-3777(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 810-1464. Strain: C57BL/6J. Tissue: Embryonic head.
[6]	"Specific hybridization probes for mouse type I, II, III and IX collagen mRNAs." Metsaeranta M., Toman D., de Crombrugghe B., Vuorio E. Biochim. Biophys. Acta 1089:241-243(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1442-1464. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52046 Genomic DNA. Translation: CAA36279.1. BC043089 mRNA. Translation: AAH43089.1. BC058724 mRNA. Translation: AAH58724.1. M18933 mRNA. Translation: AAA37338.1. K03037 Genomic DNA. No translation available. AK019448 mRNA. Translation: BAB31724.1. X57983 Genomic DNA. Translation: CAA41048.1.
IPI	IPI00129571.
PIR	A27353. S59856.
RefSeq	NP_034060.2. NM_009930.2.
UniGene	Mm.249555.
3D structure databases
ProteinModelPortal	P08121.
SMR	P08121. Positions 1247-1464.
ModBase	Search...
PTM databases
PhosphoSite	P08121.
Proteomic databases
PaxDb	P08121.
PRIDE	P08121.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087883; ENSMUSP00000085192; ENSMUSG00000026043.
GeneID	12825.
KEGG	mmu:12825.
Organism-specific databases
CTD	1281.
MGI	MGI:88453. Col3a1.
Phylogenomic databases
eggNOG	NOG12793.
HOVERGEN	HBG004933.
InParanoid	P08121.
KO	K06236.
OMA	EGSPGHP.
OrthoDB	EOG4FTW1C.
Gene expression databases
ArrayExpress	P08121.
Bgee	P08121.
CleanEx	MM_COL3A1.
Genevestigator	P08121.
Family and domain databases
InterPro	IPR008160. Collagen. IPR000885. Fib_collagen_C. IPR001007. VWF_C. [Graphical view]
Pfam	PF01410. COLFI. 1 hit. PF01391. Collagen. 7 hits. PF00093. VWC. 1 hit. [Graphical view]
ProDom	PD002078. Fib_collagen_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00038. COLFI. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
PROSITE	PS51461. NC1_FIB. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	COL3A1. mouse.
NextBio	282310.
SOURCE	Search...

Entry information

Entry name

CO3A1_MOUSE

Accession

Primary (citable) accession number: P08121
Secondary accession number(s): Q61429, Q9CRN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 15, 1999
Last modified:	May 1, 2013
This is version 128 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program