P08118 (MSMB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-microseminoprotein

Alternative name(s):
Immunoglobulin-binding factor
Short name=IGBF
PN44
Prostate secreted seminal plasma protein
Prostate secretory protein of 94 amino acids
Short name=PSP-94
Short name=PSP94
Seminal plasma beta-inhibin

Gene names

Name:	MSMB
Synonyms:	PRSP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Homodimer; Interacts with PI16. Ref.18 Ref.21
Subcellular location	Secreted. Note: Sperm surface.
Tissue specificity	Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder. Ref.6 Ref.16
Involvement in disease	Defects in MSMB are the cause of susceptibility to prostate cancer hereditary type 13 (HPC13) [MIM:611928]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Miscellaneous	Specific receptors for this protein are found on spermatozoa and in the prostate.
Sequence similarities	Belongs to the beta-microseminoprotein family.
Caution	Was originally thought to inhibit the secretion of FSH by pituitary cells.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	extracellular space Traceable author statement. Source: ProtInc nucleus Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform PSP94 (identifier: P08118-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform PSP57 (identifier: P08118-2)

The sequence of this isoform differs from the canonical sequence as follows:
     37-77: KCMDLKGNKH...SCCTLVSTPV → MFLHLWVMTK...QKRPVLSVNG
     78-114: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

Chain

21 – 114

94

Beta-microseminoprotein

PRO_0000019268

Amino acid modifications

Disulfide bond

22 ↔ 70

Ref.19 Ref.21

Disulfide bond

38 ↔ 62

Ref.19 Ref.21

Disulfide bond

57 ↔ 93

Ref.19 Ref.21

Disulfide bond

60 ↔ 69

Ref.19 Ref.21

Disulfide bond

84 ↔ 107

Ref.19 Ref.21

Natural variations

Alternative sequence

37 – 77

41

KCMDL…VSTPV → MFLHLWVMTKTTAKESSRRR TASISWWRRRTQKRPVLSVN G in isoform PSP57.

VSP_003275

Alternative sequence

78 – 114

37

Missing in isoform PSP57.

VSP_003276

Natural variant

17

1

L → S.
Corresponds to variant rs1804776 [ dbSNP | Ensembl ].

VAR_011935

Natural variant

25

1

I → M.
Corresponds to variant rs1804778 [ dbSNP | Ensembl ].

VAR_011936

Natural variant

52

1

W → R.
Corresponds to variant rs1804780 [ dbSNP | Ensembl ].

VAR_011937

Natural variant

53

1

Q → R.
Corresponds to variant rs1804468 [ dbSNP | Ensembl ].

VAR_011938

Natural variant

80

1

D → A.
Corresponds to variant rs1802774 [ dbSNP | Ensembl ].

VAR_011939

Natural variant

87

1

I → T.
Corresponds to variant rs1802771 [ dbSNP | Ensembl ].

VAR_011940

Natural variant

91

1

E → G.
Corresponds to variant rs1804469 [ dbSNP | Ensembl ].

VAR_011941

Natural variant

92

1

D → G.
Corresponds to variant rs1804461 [ dbSNP | Ensembl ].

VAR_011942

Natural variant

98

1

V → L.
Corresponds to variant rs1804464 [ dbSNP | Ensembl ].

VAR_011943

Experimental info

Sequence conflict

113

1

I → G AA sequence Ref.14

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

114

Helix Strand Turn

Details...

Beta strand

22 – 26

5

Beta strand

34 – 37

4

Beta strand

51 – 54

4

Turn

55 – 57

3

Beta strand

58 – 63

6

Beta strand

66 – 72

7

Beta strand

75 – 79

5

Turn

81 – 83

3

Beta strand

84 – 89

6

Helix

90 – 92

3

Beta strand

94 – 101

8

Beta strand

110 – 113

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform PSP94 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: D0B13E436D70BB1D	FASTA	114	12,865
	10 20 30 40 50 60 MNVLLGSVVI FATFVTLCNA SCYFIPNEGV PGDSTRKCMD LKGNKHPINS EWQTDNCETC 70 80 90 100 110 TCYETEISCC TLVSTPVGYD KDNCQRIFKK EDCKYIVVEK KDPKKTCSVS EWII
	Isoform PSP57 [UniParc]. Checksum: ADF30AB8D7A63C9A	FASTA	77	8,778
	10 20 30 40 50 60 MNVLLGSVVI FATFVTLCNA SCYFIPNEGV PGDSTRMFLH LWVMTKTTAK ESSRRRTASI 70 SWWRRRTQKR PVLSVNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence of the cDNA for a 94-amino-acid seminal plasma protein secreted by the human prostate." Mbikay M., Nolet S., Fournier S., Benjannet S., Chapdelaine P., Paradis G., Dube J.Y., Tremblay R., Lazure C., Seidah N.G., Chretien M. DNA 6:23-29(1987) [PubMed: 3829888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PSP94).
[2]	"Molecular cloning of a small prostate protein, known as beta-microsemenoprotein, PSP94 or beta-inhibin, and demonstration of transcripts in non-genital tissues." Ulvsbaeck M., Lindstroem C., Weiber H., Abrahamsson P.-A., Lilja H., Lundwall A. Biochem. Biophys. Res. Commun. 164:1310-1315(1989) [PubMed: 2590204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PSP94).
[3]	"Cloning and nucleotide sequence analysis of the human beta-microseminoprotein gene." Green C.B., Liu W.Y., Kwok S.C.M. Biochem. Biophys. Res. Commun. 167:1184-1190(1990) [PubMed: 2322265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Prostatic secretory protein PSP94: gene organization and promoter sequence in rhesus monkey and human." Nolet S., Mbikay M., Chretien M. Biochim. Biophys. Acta 1089:247-249(1991) [PubMed: 2054385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Decreased expression of prostatic secretory protein PSP94 in prostate cancer." Liu A.Y., Bradner R.C., Vessella R.L. Cancer Lett. 74:91-99(1993) [PubMed: 7506990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PSP94). Tissue: Prostate.
[6]	"Alternative splicing of PSP94 (prostatic secretory protein of 94 amino acids) mRNA in prostate tissue." Xuan J.W., Chin J.L., Guo Y., Chambers A.F., Finkelman M.A., Clarke M.W. Oncogene 11:1041-1047(1995) [PubMed: 7566962] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PSP57), TISSUE SPECIFICITY. Tissue: Prostate.
[7]	"Prostate specific protein (PSP94) expression in a human endometrial cell line (KLE)." Baijal-Gupta M., Clarke M.W. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PSP94).
[8]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PSP94).
[9]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PSP94).
[10]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PSP94). Tissue: Prostate.
[12]	"New world, but not old world, monkeys carry several genes encoding beta-microseminoprotein." Maekinen M., Valtonen-Andre C., Lundwall A. Eur. J. Biochem. 264:407-414(1999) [PubMed: 10491085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-72.
[13]	"The amino acid sequence of human beta-microseminoprotein." Akiyama K., Yoshioka Y., Schmid K., Offner G.D., Troxler R.F., Tsuda R., Hara M. Biochim. Biophys. Acta 829:288-294(1985) [PubMed: 3995056] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-113.
[14]	"Complete amino acid sequence of human seminal plasma beta-inhibin. Prediction of post Gln-Arg cleavage as a maturation site." Seidah N.G., Arbatti N.J., Rochemont J., Sheth A.R., Chretien M. FEBS Lett. 175:349-355(1984) [PubMed: 6434350] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-114.
[15]	"Structural identity of immunoglobulin binding factor and prostatic secretory protein of human seminal plasma." Liang Z.G., Kamada M., Koide S.S. Biochem. Biophys. Res. Commun. 180:356-359(1991) [PubMed: 1930232] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-50 AND 113-114.
[16]	"Human seminal plasma beta-microseminoprotein: its purification, characterization, and immunohistochemical localization." Ohkubo I., Tada T., Ochiai Y., Ueyama H., Eimoto T., Sasaki M. Int. J. Biochem. Cell Biol. 27:603-611(1995) [PubMed: 7671139] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-41, TISSUE SPECIFICITY. Tissue: Semen.
[17]	"Newly identified proteins in human nasal lavage fluid from non-smokers and smokers using two-dimensional gel electrophoresis and peptide mass fingerprinting." Ghafouri B., Stahlbom B., Tagesson C., Lindahl M. Proteomics 2:112-120(2002) [PubMed: 11788998] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-32.
[18]	"Identification, purification and characterization of a novel human blood protein with binding affinity for prostate secretory protein of 94 amino acids." Reeves J.R., Xuan J.W., Arfanis K., Morin C., Garde S.V., Ruiz M.T., Wisniewski J., Panchal C., Tanner J.E. Biochem. J. 385:105-114(2005) [PubMed: 15344909] [Abstract] Cited for: INTERACTION WITH PI16.
[19]	"Solution structures of human and porcine beta-microseminoprotein." Ghasriani H., Teilum K., Johnsson Y., Fernlund P., Drakenberg T. J. Mol. Biol. 362:502-515(2006) [PubMed: 16930619] [Abstract] Cited for: STRUCTURE BY NMR OF 21-114, DISULFIDE BONDS.
[20]	"Multiple loci identified in a genome-wide association study of prostate cancer." Thomas G., Jacobs K.B., Yeager M., Kraft P., Wacholder S., Orr N., Yu K., Chatterjee N., Welch R., Hutchinson A., Crenshaw A., Cancel-Tassin G., Staats B.J., Wang Z., Gonzalez-Bosquet J., Fang J., Deng X., Berndt S.I. , Calle E.E., Feigelson H.S., Thun M.J., Rodriguez C., Albanes D., Virtamo J., Weinstein S., Schumacher F.R., Giovannucci E., Willett W.C., Cussenot O., Valeri A., Andriole G.L., Crawford E.D., Tucker M., Gerhard D.S., Fraumeni J.F. Jr., Hoover R., Hayes R.B., Hunter D.J., Chanock S.J. Nat. Genet. 40:310-315(2008) [PubMed: 18264096] [Abstract] Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO HEREDITARY PROSTATE CANCER.
[21]	"Crystal structure of prostate secretory protein PSP94 shows an edge-to-edge association of two monomers to form a homodimer." Kumar A., Jagtap D.D., Mahale S.D., Kumar M. J. Mol. Biol. 397:947-956(2010) [PubMed: 20184897] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 21-114, SUBUNIT, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15885 mRNA. Translation: AAA36635.1.
M34376 , M34373, M34374, M34375 Genomic DNA. Translation: AAA59871.1.
X57928 , X57929, X57930, X57931 Genomic DNA. Translation: CAA41002.1.
S67815 mRNA. Translation: AAB29732.1.
U22178 mRNA. Translation: AAA83556.1.
U78976 mRNA. Translation: AAB37355.1.
BT006816 mRNA. Translation: AAP35462.1.
CR457015 mRNA. Translation: CAG33296.1.
AL450342 Genomic DNA. Translation: CAI14475.1.
AL450342 Genomic DNA. Translation: CAI14476.1.
BC005257 mRNA. Translation: AAH05257.1.
AJ133356 Genomic DNA. Translation: CAB39325.1.

IPI

IPI00218911.
IPI00414609.

PIR

A34567.
G01730.

RefSeq

NP_002434.1. NM_002443.3.
NP_619540.1. NM_138634.2.

UniGene

Hs.255462.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IZ3	NMR	-	A	21-114	[»]
3IX0	X-ray	2.30	A/B/C/D	21-114	[»]

ProteinModelPortal

P08118.

SMR

P08118. Positions 21-114.

ModBase

Search...

Protein-protein interaction databases

STRING

P08118.

Polymorphism databases

DMDM

131436.

Proteomic databases

PeptideAtlas

P08118.

PRIDE

P08118.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000358559; ENSP00000351363; ENSG00000138294.

GeneID

4477.

KEGG

hsa:4477.

UCSC

uc001jiq.1. human.
uc001jir.1. human.

Organism-specific databases

CTD

4477.

GeneCards

GC10P051549.

H-InvDB

HIX0008822.

HGNC

HGNC:7372. MSMB.

HPA

CAB026357.

MIM

157145. gene.
611928. phenotype.

neXtProt

NX_P08118.

Orphanet

1331. Familial prostate cancer.

PharmGKB

PA31177.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20970.

GeneTree

ENSGT00390000010587.

HOGENOM

HBG282389.

HOVERGEN

HBG031716.

InParanoid

P08118.

OMA

CKYIVVE.

OrthoDB

EOG4D7Z79.

PhylomeDB

P08118.

Gene expression databases

ArrayExpress

P08118.

Bgee

P08118.

CleanEx

HS_MSMB.

Genevestigator

P08118.

GermOnline

ENSG00000138294. Homo sapiens.

Family and domain databases

InterPro

IPR008735. PSP94.
[Graphical view]

PANTHER

PTHR10500. PSP94. 1 hit.

Pfam

PF05825. PSP94. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

17325.

SOURCE

Search...

Entry information

Entry name

MSMB_HUMAN

Accession

Primary (citable) accession number: P08118
Secondary accession number(s): B1API6 , P11999, Q13125, Q6IAY9, Q9UC59

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	January 25, 2012
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.