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P08117 (AMY3_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-amylase AMY3
EC=3.2.1.1
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene names
Name:AMY1.1
Synonyms:ALPHA-AMY3
OrganismTriticum aestivum (Wheat)
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Important for breakdown of endosperm starch during germination.

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactor

Binds 3 calcium ions per subunit By similarity.

Subunit structure

Monomer By similarity.

Developmental stage

Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.

Miscellaneous

Binds starch not only at the active site, but also via accessory binding sites on the protein surface that are important for efficient binding to starch granules and thereby increase enzyme activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Germination
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-amylase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 413389Alpha-amylase AMY3
PRO_0000001418

Regions

Region76 – 772Substrate binding By similarity
Region201 – 2066Substrate binding By similarity
Region282 – 2843Substrate binding By similarity
Region385 – 3873Substrate binding By similarity

Sites

Active site2031Nucleophile By similarity
Active site2281Proton donor By similarity
Metal binding1151Calcium 1 By similarity
Metal binding1321Calcium 2 By similarity
Metal binding1351Calcium 2; via carbonyl oxygen By similarity
Metal binding1371Calcium 2; via carbonyl oxygen By similarity
Metal binding1411Calcium 2 By similarity
Metal binding1511Calcium 3 By similarity
Metal binding1621Calcium 1 By similarity
Metal binding1671Calcium 3; via carbonyl oxygen By similarity
Metal binding1701Calcium 3; via carbonyl oxygen By similarity
Metal binding1721Calcium 1 By similarity
Metal binding1721Calcium 3 By similarity
Metal binding2071Calcium 1; via carbonyl oxygen By similarity
Binding site2351Substrate By similarity
Binding site2761Substrate By similarity
Binding site2951Substrate By similarity
Binding site3011Substrate By similarity
Binding site3801Substrate By similarity
Binding site4081Substrate By similarity
Site2961Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P08117 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: C262ECA1C54FCCE4

FASTA41345,370
        10         20         30         40         50         60 
MGKHSATLCG LLVVVLCLAS SLAQAQILFQ GFNWESWKTQ GGWYKFMQGK VEEIASTGAT 

        70         80         90        100        110        120 
HVWLPPPSQS VSPEGYLPGQ LYNLNSKYGS GADLKSLIQA FRGKNISCVA DIVINHRCAD 

       130        140        150        160        170        180 
KKDGRGVYCI FEGGTSDNRL DWGPDEICSD DTKYSNGRGH RDTGGGFDAA PDIDHLNPRV 

       190        200        210        220        230        240 
QRELSAWLNW LKTDLGFDGW RLDFAKGYSA AMAKIYVDNS KPAFVVGELY DRDRQLLANW 

       250        260        270        280        290        300 
VRGVGGPATA FDFPTKGVLQ EAVQGDLGRM RGSDGKAPGM IGWMPEKTVT FIDNHDTGST 

       310        320        330        340        350        360 
QRLWPFPSDK VMQGYAYILT HPGIPCIFYD HVFDWKLKQE ITALATVRSR NGIHPGSTLD 

       370        380        390        400        410 
ILKAEGDLYV AKIGGKVITK IGSRYNIGDN VIPSGFKIAA KGNNYCVWEK SGL

« Hide

References

[1]"A novel wheat alpha-amylase gene (alpha-Amy3)."
Baulcombe D.C., Huttly A.K., Martienssen R.A., Barker R.F., Jarvis M.G.
Mol. Gen. Genet. 209:33-40(1987) [PubMed: 17186618] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Chinese Spring.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X05809 Genomic DNA. Translation: CAA29252.1.
M16991 Genomic DNA. Translation: AAA34259.1.
PIRALWT3. S06357.

3D structure databases

ProteinModelPortalP08117.
SMRP08117. Positions 26-410.
ModBaseSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP08117.

Family and domain databases

InterProIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR015902. Alpha_amylase.
IPR006046. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHERPTHR10357. Alpha_amylase. 1 hit.
PfamPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMY3_WHEAT
AccessionPrimary (citable) accession number: P08117
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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