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P08113

- ENPL_MOUSE

UniProt

P08113 - ENPL_MOUSE

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Protein

Endoplasmin

Gene

Hsp90b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATPBy similarity
Binding sitei149 – 1491ATPBy similarity
Binding sitei162 – 1621ATPBy similarity
Binding sitei168 – 1681ATPBy similarity
Binding sitei199 – 1991ATP; via amide nitrogenBy similarity
Binding sitei448 – 4481ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. low-density lipoprotein particle receptor binding Source: MGI
  3. protein phosphatase binding Source: MGI
  4. RNA binding Source: Ensembl
  5. virion binding Source: Ensembl

GO - Biological processi

  1. actin rod assembly Source: MGI
  2. cellular response to ATP Source: MGI
  3. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  4. negative regulation of apoptotic process Source: Ensembl
  5. protein folding Source: InterPro
  6. regulation of phosphoprotein phosphatase activity Source: MGI
  7. response to hypoxia Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196581. Scavenging by Class A Receptors.
REACT_198976. Trafficking and processing of endosomal TLR.
REACT_32838. ATF6-alpha activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
Endoplasmic reticulum resident protein 99
Short name:
ERp99
Heat shock protein 90 kDa beta member 1
Polymorphic tumor rejection antigen 1
Tumor rejection antigen gp96
Gene namesi
Name:Hsp90b1
Synonyms:Grp94, Tra-1, Tra1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:98817. Hsp90b1.

Subcellular locationi

Endoplasmic reticulum lumen 1 Publication. Melanosome By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. endoplasmic reticulum Source: MGI
  3. endoplasmic reticulum lumen Source: Ensembl
  4. endoplasmic reticulum membrane Source: Ensembl
  5. extracellular region Source: Reactome
  6. extracellular vesicular exosome Source: Ensembl
  7. midbody Source: Ensembl
  8. nucleus Source: Ensembl
  9. perinuclear region of cytoplasm Source: Ensembl
  10. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi103 – 1031E → A: Loss of CNPY3-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 802781EndoplasminPRO_0000013599Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi138 – 138Interchain
Glycosylationi217 – 2171N-linked (GlcNAc...)1 Publication
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei404 – 4041N6-succinyllysine1 Publication
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
Modified residuei479 – 4791N6-acetyllysine1 Publication
Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
Modified residuei633 – 6331N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP08113.
PaxDbiP08113.
PRIDEiP08113.

2D gel databases

REPRODUCTION-2DPAGEP08113.
SWISS-2DPAGEP08113.

PTM databases

PhosphoSiteiP08113.

Miscellaneous databases

PMAP-CutDBP08113.

Expressioni

Gene expression databases

BgeeiP08113.
CleanExiMM_HSP90B1.
ExpressionAtlasiP08113. baseline and differential.
GenevestigatoriP08113.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with OS9 (By similarity). Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with TLR4, TLR9 and TLR11, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner. Interacts with METTL23 (By similarity).By similarity

Protein-protein interaction databases

BioGridi204301. 8 interactions.
DIPiDIP-32352N.
IntActiP08113. 11 interactions.
MINTiMINT-1863762.

Structurei

3D structure databases

ProteinModelPortaliP08113.
SMRiP08113. Positions 72-755.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi799 – 8024Prevents secretion from ER

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0326.
HOGENOMiHOG000031988.
HOVERGENiHBG007374.
InParanoidiP08113.
KOiK09487.
OMAiQSSHHPS.
OrthoDBiEOG780RM0.
PhylomeDBiP08113.
TreeFamiTF105969.

Family and domain databases

Gene3Di3.30.565.10. 2 hits.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08113-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ
60 70 80 90 100
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF
110 120 130 140 150
LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT
160 170 180 190 200
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY
210 220 230 240 250
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV
260 270 280 290 300
LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDEAAK
310 320 330 340 350
EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV
360 370 380 390 400
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE
410 420 430 440 450
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET
460 470 480 490 500
LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH
510 520 530 540 550
SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE
560 570 580 590 600
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE
610 620 630 640 650
SEKTKESREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS
660 670 680 690 700
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR
710 720 730 740 750
IKEDEDDKTV MDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID
760 770 780 790 800
PEAQVEEEPE EEPEDTSEDA EDSEQDEGEE MDAGTEEEEE ETEKESTEKD

EL
Length:802
Mass (Da):92,476
Last modified:April 1, 1990 - v2
Checksum:iBDF33B9BD86BFC5F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03297 mRNA. Translation: AAA37573.1.
M29652 mRNA. Translation: AAA37743.1.
M16370 mRNA. Translation: AAA40023.1.
CCDSiCCDS36019.1.
PIRiA29317.
RefSeqiNP_035761.1. NM_011631.1.
UniGeneiMm.87773.

Genome annotation databases

EnsembliENSMUST00000020238; ENSMUSP00000020238; ENSMUSG00000020048.
GeneIDi22027.
KEGGimmu:22027.
UCSCiuc007gqi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03297 mRNA. Translation: AAA37573.1 .
M29652 mRNA. Translation: AAA37743.1 .
M16370 mRNA. Translation: AAA40023.1 .
CCDSi CCDS36019.1.
PIRi A29317.
RefSeqi NP_035761.1. NM_011631.1.
UniGenei Mm.87773.

3D structure databases

ProteinModelPortali P08113.
SMRi P08113. Positions 72-755.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204301. 8 interactions.
DIPi DIP-32352N.
IntActi P08113. 11 interactions.
MINTi MINT-1863762.

PTM databases

PhosphoSitei P08113.

2D gel databases

REPRODUCTION-2DPAGE P08113.
SWISS-2DPAGE P08113.

Proteomic databases

MaxQBi P08113.
PaxDbi P08113.
PRIDEi P08113.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000020238 ; ENSMUSP00000020238 ; ENSMUSG00000020048 .
GeneIDi 22027.
KEGGi mmu:22027.
UCSCi uc007gqi.1. mouse.

Organism-specific databases

CTDi 7184.
MGIi MGI:98817. Hsp90b1.

Phylogenomic databases

eggNOGi COG0326.
HOGENOMi HOG000031988.
HOVERGENi HBG007374.
InParanoidi P08113.
KOi K09487.
OMAi QSSHHPS.
OrthoDBi EOG780RM0.
PhylomeDBi P08113.
TreeFami TF105969.

Enzyme and pathway databases

Reactomei REACT_196581. Scavenging by Class A Receptors.
REACT_198976. Trafficking and processing of endosomal TLR.
REACT_32838. ATF6-alpha activates chaperone genes.

Miscellaneous databases

ChiTaRSi HSP90B1. mouse.
NextBioi 301772.
PMAP-CutDB P08113.
PROi P08113.
SOURCEi Search...

Gene expression databases

Bgeei P08113.
CleanExi MM_HSP90B1.
ExpressionAtlasi P08113. baseline and differential.
Genevestigatori P08113.

Family and domain databases

Gene3Di 3.30.565.10. 2 hits.
HAMAPi MF_00505. HSP90.
InterProi IPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view ]
PANTHERi PTHR11528. PTHR11528. 1 hit.
Pfami PF00183. HSP90. 1 hit.
[Graphical view ]
PIRSFi PIRSF002583. Hsp90. 1 hit.
PRINTSi PR00775. HEATSHOCK90.
SMARTi SM00387. HATPase_c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ERp99, an abundant, conserved glycoprotein of the endoplasmic reticulum, is homologous to the 90-kDa heat shock protein (hsp90) and the 94-kDa glucose regulated protein (GRP94)."
    Mazzarella R.A., Green M.
    J. Biol. Chem. 262:8875-8883(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 76-84; 88-95; 117-135; 143-156; 244-265; 270-285; 385-404; 416-428; 435-448; 512-530; 609-623 AND 640-660, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Isolation and identification of partial cDNA clones for endoplasmin, the major glycoprotein of mammalian endoplasmic reticulum."
    Smith M.J., Koch G.L.E.
    J. Mol. Biol. 194:345-347(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 378-507.
  4. "5'-structural analysis of genes encoding polymorphic antigens of chemically induced tumors."
    Srivastava P.K., Chen Y.-T., Old L.J.
    Proc. Natl. Acad. Sci. U.S.A. 84:3807-3811(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-88.
  5. "HSP100, a 100-kDa heat shock protein, is a Ca2+-calmodulin-regulated actin-binding protein."
    Koyasu S., Nishida E., Miyata Y., Sakai H., Yahara I.
    J. Biol. Chem. 264:15083-15087(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 271-284; 328-339 AND 633-659.
  6. "Analysis of the structure and synthesis of GRP94, an abundant stress protein of the endoplasmic reticulum."
    Qu D., Mazzarella R.A., Green M.
    DNA Cell Biol. 13:117-124(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERCHAIN DISULFIDE BOND.
  7. "A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins."
    Meunier L., Usherwood Y.-K., Chung K.T., Hendershot L.M.
    Mol. Biol. Cell 13:4456-4469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF A CHAPERONE COMPLEX.
  8. "Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver."
    Timchenko L.T., Salisbury E., Wang G.-L., Nguyen H., Albrecht J.H., Hershey J.W., Timchenko N.A.
    J. Biol. Chem. 281:32806-32819(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN AN EIF2 COMPLEX WITH EIF2S1; EIF2S2; CELF1; CALR3; HSPA5 AND CALR.
  9. "Mzb1 protein regulates calcium homeostasis, antibody secretion, and integrin activation in innate-like B cells."
    Flach H., Rosenbaum M., Duchniewicz M., Kim S., Zhang S.L., Cahalan M.D., Mittler G., Grosschedl R.
    Immunity 33:723-735(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MZB1.
  10. "Folding of Toll-like receptors by the HSP90 paralogue gp96 requires a substrate-specific cochaperone."
    Liu B., Yang Y., Qiu Z., Staron M., Hong F., Li Y., Wu S., Li Y., Hao B., Bona R., Han D., Li Z.
    Nat. Commun. 1:79-79(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CNPY3; TLR4; TLR9 AND TLR11, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-103.
  11. Erratum
    Liu B., Yang Y., Qiu Z., Staron M., Hong F., Li Y., Wu S., Li Y., Hao B., Bona R., Han D., Li Z.
    Nat. Commun. 3:653-653(2012)
  12. "Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96: its pathological implications in lupus-like autoimmune diseases."
    Han J.M., Park S.G., Liu B., Park B.-J., Kim J.Y., Jin C.H., Song Y.W., Li Z., Kim S.
    Am. J. Pathol. 170:2042-2054(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AIMP1.
  13. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-217.
  14. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-479, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-404 AND LYS-633, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiENPL_MOUSE
AccessioniPrimary (citable) accession number: P08113
Secondary accession number(s): P11427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3