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Protein

Endoplasmin

Gene

Hsp90b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPBy similarity1
Binding sitei149ATPBy similarity1
Binding sitei162ATPBy similarity1
Binding sitei168ATPBy similarity1
Binding sitei199ATP; via amide nitrogenBy similarity1
Binding sitei448ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1679131. Trafficking and processing of endosomal TLR.
R-MMU-3000480. Scavenging by Class A Receptors.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-MMU-8957275. Post-translational protein phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
Endoplasmic reticulum resident protein 99
Short name:
ERp99
Heat shock protein 90 kDa beta member 1
Polymorphic tumor rejection antigen 1
Tumor rejection antigen gp96
Gene namesi
Name:Hsp90b1
Synonyms:Grp94, Tra-1, Tra1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:98817. Hsp90b1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103E → A: Loss of CNPY3-binding. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3425396.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001359922 – 802EndoplasminAdd BLAST781

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei64PhosphoserineBy similarity1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi138Interchain
Modified residuei172PhosphoserineBy similarity1
Glycosylationi217N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei306PhosphoserineBy similarity1
Modified residuei403PhosphoserineBy similarity1
Modified residuei404N6-succinyllysineCombined sources1
Glycosylationi445N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei447PhosphoserineBy similarity1
Modified residuei479N6-acetyllysineCombined sources1
Glycosylationi481N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi502N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei633N6-succinyllysineCombined sources1
Modified residuei785PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP08113.
PaxDbiP08113.
PeptideAtlasiP08113.
PRIDEiP08113.

2D gel databases

REPRODUCTION-2DPAGEiP08113.
SWISS-2DPAGEiP08113.

PTM databases

iPTMnetiP08113.
PhosphoSitePlusiP08113.
SwissPalmiP08113.

Miscellaneous databases

PMAP-CutDBiP08113.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020048.
CleanExiMM_HSP90B1.
ExpressionAtlasiP08113. baseline and differential.
GenevisibleiP08113. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with OS9 (By similarity). Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with TLR4, TLR9 and TLR11, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner. Interacts with METTL23 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204301. 9 interactors.
CORUMiP08113.
DIPiDIP-32352N.
IntActiP08113. 16 interactors.
MINTiMINT-1863762.
STRINGi10090.ENSMUSP00000020238.

Structurei

3D structure databases

ProteinModelPortaliP08113.
SMRiP08113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi799 – 802Prevents secretion from ER4

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
GeneTreeiENSGT00900000140978.
HOGENOMiHOG000031988.
HOVERGENiHBG007374.
InParanoidiP08113.
KOiK09487.
OMAiSDHEIFL.
OrthoDBiEOG091G0270.
PhylomeDBiP08113.
TreeFamiTF105969.

Family and domain databases

CDDicd00075. HATPase_c. 1 hit.
Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiView protein in InterPro
IPR003594. HATPase_C.
IPR036890. HATPase_C_sf.
IPR019805. Heat_shock_protein_90_CS.
IPR037196. HSP90_C.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SUPFAMiSSF110942. SSF110942. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiView protein in PROSITE
PS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ
60 70 80 90 100
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF
110 120 130 140 150
LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT
160 170 180 190 200
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY
210 220 230 240 250
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV
260 270 280 290 300
LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDEAAK
310 320 330 340 350
EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV
360 370 380 390 400
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE
410 420 430 440 450
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET
460 470 480 490 500
LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH
510 520 530 540 550
SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE
560 570 580 590 600
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE
610 620 630 640 650
SEKTKESREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS
660 670 680 690 700
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR
710 720 730 740 750
IKEDEDDKTV MDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID
760 770 780 790 800
PEAQVEEEPE EEPEDTSEDA EDSEQDEGEE MDAGTEEEEE ETEKESTEKD

EL
Length:802
Mass (Da):92,476
Last modified:April 1, 1990 - v2
Checksum:iBDF33B9BD86BFC5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03297 mRNA. Translation: AAA37573.1.
M29652 mRNA. Translation: AAA37743.1.
M16370 mRNA. Translation: AAA40023.1.
CCDSiCCDS36019.1.
PIRiA29317.
RefSeqiNP_035761.1. NM_011631.1.
UniGeneiMm.87773.

Genome annotation databases

EnsembliENSMUST00000020238; ENSMUSP00000020238; ENSMUSG00000020048.
GeneIDi22027.
KEGGimmu:22027.
UCSCiuc007gqi.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiENPL_MOUSE
AccessioniPrimary (citable) accession number: P08113
Secondary accession number(s): P11427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 1, 1990
Last modified: October 25, 2017
This is version 183 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families