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Protein

Lethal(2) giant larvae protein

Gene

l(2)gl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the development of polarized epithelia, for cell polarity associated with asymmetric cell division of neuroblasts during development, and for oocyte polarity formation. Promotes the formation of actin-rich projections at the oocyte cortex and the posterior enrichment of par-1 which is required for oocyte polarization. Regulates the localization of axis-specifying morphogens such as stau and grk.
Isoform p78: Has an essential role in control of cell proliferation and differentiation during development and could act as a tumor suppressor.
Isoform p127: Has an accessory function in control of cell proliferation and differentiation during development.

GO - Molecular functioni

  • GTPase activator activity Source: GO_Central
  • myosin binding Source: FlyBase
  • myosin II binding Source: FlyBase
  • protein kinase inhibitor activity Source: FlyBase
  • Rab GTPase binding Source: GO_Central
  • SNARE binding Source: FlyBase
  • syntaxin binding Source: GO_Central

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • apical protein localization Source: FlyBase
  • asymmetric neuroblast division Source: FlyBase
  • asymmetric protein localization Source: FlyBase
  • asymmetric protein localization involved in cell fate determination Source: FlyBase
  • basal protein localization Source: FlyBase
  • cell competition in a multicellular organism Source: FlyBase
  • cell proliferation Source: FlyBase
  • chitin-based larval cuticle pattern formation Source: FlyBase
  • cortical actin cytoskeleton organization Source: GO_Central
  • dorsal closure Source: FlyBase
  • ecdysone-mediated induction of salivary gland cell autophagic cell death Source: FlyBase
  • epidermis morphogenesis Source: FlyBase
  • epithelium development Source: FlyBase
  • establishment of epithelial cell planar polarity Source: FlyBase
  • establishment of mitotic spindle orientation Source: FlyBase
  • establishment of neuroblast polarity Source: FlyBase
  • establishment of protein localization Source: FlyBase
  • establishment or maintenance of epithelial cell apical/basal polarity Source: FlyBase
  • establishment or maintenance of neuroblast polarity Source: FlyBase
  • establishment or maintenance of polarity of embryonic epithelium Source: FlyBase
  • establishment or maintenance of polarity of follicular epithelium Source: FlyBase
  • establishment or maintenance of polarity of larval imaginal disc epithelium Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • localization within membrane Source: FlyBase
  • morphogenesis of an epithelium Source: FlyBase
  • morphogenesis of a polarized epithelium Source: FlyBase
  • morphogenesis of follicular epithelium Source: FlyBase
  • morphogenesis of larval imaginal disc epithelium Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • negative regulation of neuroblast proliferation Source: FlyBase
  • negative regulation of Notch signaling pathway Source: FlyBase
  • negative regulation of protein kinase activity Source: FlyBase
  • nervous system development Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • oocyte anterior/posterior axis specification Source: FlyBase
  • oocyte microtubule cytoskeleton organization Source: FlyBase
  • protein localization Source: FlyBase
  • protein localization to cell cortex Source: FlyBase
  • regulation of cell cycle Source: FlyBase
  • regulation of cell shape Source: FlyBase
  • regulation of establishment or maintenance of cell polarity Source: GO_Central
  • regulation of protein secretion Source: GO_Central
  • salivary gland histolysis Source: FlyBase
  • sensory organ development Source: FlyBase
  • septate junction assembly Source: FlyBase
  • symmetric cell division Source: FlyBase
  • synaptic vesicle priming Source: FlyBase
  • transforming growth factor beta receptor signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Exocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Lethal(2) giant larvae protein
Gene namesi
Name:l(2)gl
ORF Names:CG2671
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0002121. l(2)gl.

Subcellular locationi

GO - Cellular componenti

  • apicolateral plasma membrane Source: FlyBase
  • basolateral plasma membrane Source: FlyBase
  • cell cortex Source: FlyBase
  • cortical actin cytoskeleton Source: GO_Central
  • cortical cytoskeleton Source: FlyBase
  • cytoplasm Source: FlyBase
  • cytoskeleton Source: FlyBase
  • cytosol Source: FlyBase
  • plasma membrane Source: FlyBase
  • proteinaceous extracellular matrix Source: FlyBase
  • septate junction Source: FlyBase
  • smooth septate junction Source: FlyBase
  • synaptic vesicle Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843451 – 1161Lethal(2) giant larvae proteinAdd BLAST1161

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei473Phosphoserine1 Publication1
Modified residuei484Phosphoserine1 Publication1
Modified residuei679Phosphoserine1 Publication1
Modified residuei808Phosphoserine1 Publication1
Modified residuei869Phosphoserine1 Publication1
Modified residuei876Phosphoserine1 Publication1
Modified residuei887Phosphoserine1 Publication1
Modified residuei889Phosphoserine1 Publication1
Modified residuei893Phosphoserine1 Publication1
Modified residuei1013Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by aPKC which restricts l2gl activity to the oocyte posterior and is required for oocyte polarity formation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08111.
PRIDEiP08111.

PTM databases

iPTMnetiP08111.

Expressioni

Tissue specificityi

Expressed in the epithelial cells of the digestive tract and in gonads.

Developmental stagei

Expressed abundantly in early embryogenesis. Moderate expression is found in larval and adult stages.

Gene expression databases

BgeeiFBgn0002121.
ExpressionAtlasiP08111. baseline.
GenevisibleiP08111. DM.

Interactioni

Subunit structurei

May form multimeric complexes. Interacts with mahj. Interacts with aPKC; the interaction results in phosphorylation of l2gl.2 Publications

GO - Molecular functioni

  • myosin binding Source: FlyBase
  • myosin II binding Source: FlyBase
  • Rab GTPase binding Source: GO_Central
  • SNARE binding Source: FlyBase
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

BioGridi59421. 9 interactors.
DIPiDIP-22687N.
IntActiP08111. 5 interactors.
MINTiMINT-778782.
STRINGi7227.FBpp0297544.

Structurei

3D structure databases

ProteinModelPortaliP08111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati39 – 72WD 1Add BLAST34
Repeati82 – 128WD 2Add BLAST47
Repeati131 – 167WD 3Add BLAST37
Repeati189 – 223WD 4Add BLAST35
Repeati231 – 263WD 5Add BLAST33
Repeati278 – 320WD 6Add BLAST43
Repeati328 – 358WD 7Add BLAST31
Repeati380 – 464WD 8Add BLAST85
Repeati513 – 594WD 9Add BLAST82
Repeati603 – 664WD 10Add BLAST62
Repeati708 – 778WD 11Add BLAST71
Repeati787 – 832WD 12Add BLAST46
Repeati837 – 927WD 13Add BLAST91
Repeati941 – 964WD 14Add BLAST24

Sequence similaritiesi

Belongs to the WD repeat L(2)GL family.Curated
Contains 14 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1983. Eukaryota.
ENOG410XS6Z. LUCA.
GeneTreeiENSGT00390000000018.
InParanoidiP08111.
KOiK06094.
OMAiGHLRDPT.
OrthoDBiEOG091G01L0.
PhylomeDBiP08111.

Family and domain databases

Gene3Di2.130.10.10. 4 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SUPFAMiSSF50978. SSF50978. 8 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform p127 (identifier: P08111-1) [UniParc]FASTAAdd to basket
Also known as: A, C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKFIRGKGQ QPSADRHRLQ KDLFAYRKTA QHGFPHKPSA LAYDPVLKLM
60 70 80 90 100
AIGTQTGALK VFGQPGVELY GQHTLLNNSA SELNVQLLEW VYGTGRILSL
110 120 130 140 150
TAANQLILWE PVGATLLPIK TLPFDGKLKK VSSLCCSLSK DLLWIGTEGG
160 170 180 190 200
NIYQLDLHTF TIKEPVIYHD VVLEQVPPAY KLNPGAIESI RQLPNSPSKL
210 220 230 240 250
LVAYNRGLCV LWDFESASVQ RAYIAPGHGQ SVGLTVNFEG SEFTWYHADG
260 270 280 290 300
SYATWSIDNP EPPSNVNYVP YGPDPCKSIN RLYKGKRRSN DVIVFSGGMP
310 320 330 340 350
RSAYGDHNCV SVHASDGHKV CLDFTSKVID FFVTFENNRD VAEVLVVLLE
360 370 380 390 400
EELCAYDLTD PNICAIKAPY LHSVHASAVT CNYLASEVVQ SVYESILRAG
410 420 430 440 450
DEQDIDYSNI SWPITGGTLP DNLEESVEED ATKLYEILLT GHEDGSVKFW
460 470 480 490 500
DCTGVLLKPI YNFKTSSIFG SESDFRDDAA ADMSAEQVDE GEPPFRKSGL
510 520 530 540 550
FDPYSDDPRL AVKKIAFCPK TGQLIVGGTA GQIVIADFID LPEKVSLKYI
560 570 580 590 600
SMNLVSDRDG FVWKGHDQLN VRSNLLDGEA IPTTERGVNI SGVLQVLPPA
610 620 630 640 650
SITCMALEAS WGLVSGGTAH GLVLFDFKNF VPVFHRCTLN PNDLTGAGEQ
660 670 680 690 700
LSRRKSFKKS LRESFRKLRK GRSTRTNQSN QVPTTLEARP VERQIEARCA
710 720 730 740 750
DDGLGSMVRC LLFAKTYVTN VNITSPTLWS ATNASTVSVF LLHLPPAQTA
760 770 780 790 800
ATAVPSASGN APPHMPRRIS AQLAKEIQLK HRAPVVGISI FDQAGSPVDQ
810 820 830 840 850
LNAGENGSPP HRVLIASEEQ FKVFSLPQLK PINKYKLTAN EGARIRRIHF
860 870 880 890 900
GSFSCRISPE TLQSMHGCSP TKSTRSHGDG EADPNISGSL AVSRGDVYNE
910 920 930 940 950
TALICLTNMG DIMVLSVPEL KRQLNAAAVR REDINGVSSL CFTNSGEALY
960 970 980 990 1000
MMSSSELQRI ALATSRVVQP TGVVPVEPLE NEESVLEEND AENNKETYAC
1010 1020 1030 1040 1050
DEVVNTYEIK NPSGISICTR PAEENVGRNS VQQVNGVNIS NSPNQANETI
1060 1070 1080 1090 1100
SSSIGDITVD SVRDHLNMTT TTLCSINTEE TIGRLSVLST QTNKASTTVN
1110 1120 1130 1140 1150
MSEIPNINIS NLEDLESKRN TTETSTSSVV IKSIITNISH EKTNGDNKIG
1160
TPKTAPEESQ F
Length:1,161
Mass (Da):126,892
Last modified:February 1, 1995 - v2
Checksum:i979F73A01DA62A1A
GO
Isoform p78 (identifier: P08111-2) [UniParc]FASTAAdd to basket
Also known as: EC371

The sequence of this isoform differs from the canonical sequence as follows:
     708-708: V → F
     709-1161: Missing.

Show »
Length:708
Mass (Da):78,112
Checksum:i830226D7396A8CC5
GO
Isoform B (identifier: P08111-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-28: HRLQKDLFAYRK → QRYI

Note: No experimental confirmation available.
Show »
Length:1,153
Mass (Da):125,896
Checksum:i577E34DFAE48539C
GO
Isoform D (identifier: P08111-4) [UniParc]FASTAAdd to basket
Also known as: E, F

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:1,112
Mass (Da):121,249
Checksum:i524F615010E8B775
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2L → F in AAA28671 (PubMed:3036370).Curated1
Sequence conflicti2L → F in AAA28672 (PubMed:3036370).Curated1
Sequence conflicti37 – 38KP → A in CAA29007 (PubMed:16453778).Curated2
Sequence conflicti58A → V in ACY56896 (Ref. 6) Curated1
Sequence conflicti185G → S in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti227G → R in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti243 – 245FTW → RYL in CAA29007 (PubMed:16453778).Curated3
Sequence conflicti304Y → N in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti331F → I in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti338N → S in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti366I → T in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti388V → L in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti512 – 515VKKI → SEEN in CAA29007 (PubMed:16453778).Curated4
Sequence conflicti667 – 675KLRKGRSTR → SFARVDQTK in CAA29007 (PubMed:16453778).Curated9
Sequence conflicti727T → S in CAA29007 (PubMed:16453778).Curated1
Sequence conflicti857I → V in ACY56896 (Ref. 6) Curated1
Sequence conflicti959R → G in AAA28671 (PubMed:3036370).Curated1
Sequence conflicti1095A → G in CAA29007 (PubMed:16453778).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0217931 – 49Missing in isoform D. CuratedAdd BLAST49
Alternative sequenceiVSP_02179417 – 28HRLQK…FAYRK → QRYI in isoform B. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_004299708V → F in isoform p78. Curated1
Alternative sequenceiVSP_004300709 – 1161Missing in isoform p78. CuratedAdd BLAST453

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05426 mRNA. Translation: CAA29007.1.
M17022 Genomic DNA. Translation: AAA28671.1.
M17022 Genomic DNA. Translation: AAA28672.1.
AE014134 Genomic DNA. Translation: AAG22255.1.
AE014134 Genomic DNA. Translation: AAG22256.2.
AE014134 Genomic DNA. Translation: AAN10501.1.
AE014134 Genomic DNA. Translation: AAN10502.1.
AE014134 Genomic DNA. Translation: AAN10503.1.
AY051654 mRNA. Translation: AAK93078.1.
BT100129 mRNA. Translation: ACX85650.1.
BT100228 mRNA. Translation: ACY56896.1.
PIRiA27069.
A27868.
RefSeqiNP_001245801.1. NM_001258872.2. [P08111-1]
NP_001245802.1. NM_001258873.2. [P08111-1]
NP_001245803.1. NM_001258874.2. [P08111-1]
NP_001245804.1. NM_001258875.2. [P08111-1]
NP_523439.2. NM_078715.4. [P08111-1]
NP_722583.1. NM_164348.3. [P08111-3]
NP_722584.1. NM_164349.3. [P08111-1]
NP_722585.1. NM_164350.3. [P08111-4]
NP_722586.1. NM_164351.3. [P08111-4]
NP_722587.1. NM_164352.3. [P08111-4]
UniGeneiDm.2784.

Genome annotation databases

EnsemblMetazoaiFBtr0078170; FBpp0077828; FBgn0002121. [P08111-1]
FBtr0078171; FBpp0077829; FBgn0002121. [P08111-1]
FBtr0306589; FBpp0297544; FBgn0002121. [P08111-1]
FBtr0306590; FBpp0297545; FBgn0002121. [P08111-1]
FBtr0306591; FBpp0297546; FBgn0002121. [P08111-1]
FBtr0306592; FBpp0297547; FBgn0002121. [P08111-1]
GeneIDi33156.
KEGGidme:Dmel_CG2671.
UCSCiCG2671-RE. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05426 mRNA. Translation: CAA29007.1.
M17022 Genomic DNA. Translation: AAA28671.1.
M17022 Genomic DNA. Translation: AAA28672.1.
AE014134 Genomic DNA. Translation: AAG22255.1.
AE014134 Genomic DNA. Translation: AAG22256.2.
AE014134 Genomic DNA. Translation: AAN10501.1.
AE014134 Genomic DNA. Translation: AAN10502.1.
AE014134 Genomic DNA. Translation: AAN10503.1.
AY051654 mRNA. Translation: AAK93078.1.
BT100129 mRNA. Translation: ACX85650.1.
BT100228 mRNA. Translation: ACY56896.1.
PIRiA27069.
A27868.
RefSeqiNP_001245801.1. NM_001258872.2. [P08111-1]
NP_001245802.1. NM_001258873.2. [P08111-1]
NP_001245803.1. NM_001258874.2. [P08111-1]
NP_001245804.1. NM_001258875.2. [P08111-1]
NP_523439.2. NM_078715.4. [P08111-1]
NP_722583.1. NM_164348.3. [P08111-3]
NP_722584.1. NM_164349.3. [P08111-1]
NP_722585.1. NM_164350.3. [P08111-4]
NP_722586.1. NM_164351.3. [P08111-4]
NP_722587.1. NM_164352.3. [P08111-4]
UniGeneiDm.2784.

3D structure databases

ProteinModelPortaliP08111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59421. 9 interactors.
DIPiDIP-22687N.
IntActiP08111. 5 interactors.
MINTiMINT-778782.
STRINGi7227.FBpp0297544.

PTM databases

iPTMnetiP08111.

Proteomic databases

PaxDbiP08111.
PRIDEiP08111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078170; FBpp0077828; FBgn0002121. [P08111-1]
FBtr0078171; FBpp0077829; FBgn0002121. [P08111-1]
FBtr0306589; FBpp0297544; FBgn0002121. [P08111-1]
FBtr0306590; FBpp0297545; FBgn0002121. [P08111-1]
FBtr0306591; FBpp0297546; FBgn0002121. [P08111-1]
FBtr0306592; FBpp0297547; FBgn0002121. [P08111-1]
GeneIDi33156.
KEGGidme:Dmel_CG2671.
UCSCiCG2671-RE. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0002121. l(2)gl.

Phylogenomic databases

eggNOGiKOG1983. Eukaryota.
ENOG410XS6Z. LUCA.
GeneTreeiENSGT00390000000018.
InParanoidiP08111.
KOiK06094.
OMAiGHLRDPT.
OrthoDBiEOG091G01L0.
PhylomeDBiP08111.

Miscellaneous databases

ChiTaRSil(2)gl. fly.
GenomeRNAii33156.
PROiP08111.

Gene expression databases

BgeeiFBgn0002121.
ExpressionAtlasiP08111. baseline.
GenevisibleiP08111. DM.

Family and domain databases

Gene3Di2.130.10.10. 4 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SUPFAMiSSF50978. SSF50978. 8 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiL2GL_DROME
AccessioniPrimary (citable) accession number: P08111
Secondary accession number(s): A4UZW0
, D0IQ91, D0IQJ0, Q0E8V5, Q24377, Q8IPV4, Q8IPV5, Q9VPI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.