Reviewed, UniProtKB/Swiss-Prot P08103 (HCK_MOUSE)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase HCK
    EC=2.7.10.2
Alternative name(s):
    Hemopoietic cell kinase
    p56-HCK/p59-HCK
    B-cell/myeloid kinase
      Short name=BMK

Gene names

Name:

Hck

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Isoform p59-HCK: Membrane; Lipid-anchor. Ref.5 Isoform p56-HCK: Membrane; Lipid-anchor. Ref.5
Tissue specificity	Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
Post-translational modification	Isoform p56-HCK contains a N-myristoyl glycine at position 3 By similarity. Isoform p56-HCK contains a S-palmitoyl cysteine at position 3 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative initiation
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	defense response to Gram-positive bacterium Inferred from genetic interaction. Source: MGI phagocytosis Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform p59-HCK (identifier: P08103-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform p56-HCK (identifier: P08103-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Note: Initiator Met-1 is removed. Contains a N-myristoyl glycine at position 3 (By similarity). Contains a S-palmitoyl cysteine at position 4 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 524	523	Tyrosine-protein kinase HCK		PRO_0000024435
Regions
	Domain	76 – 136	61	SH3
	Domain	142 – 239	98	SH2
	Domain	260 – 513	254	Protein kinase
	Nucleotide binding	266 – 274	9	ATP By similarity
Sites
	Active site	379	1	Proton acceptor By similarity
	Binding site	288	1	ATP By similarity
Amino acid modifications
	Modified residue	34	1	Phosphoserine
	Modified residue	207	1	Phosphotyrosine
	Modified residue	409	1	Phosphotyrosine; by autocatalysis By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
Natural variations
	Alternative sequence	1 – 21	21	Missing in isoform p56-HCK.		VSP_018859

Sequences

	Sequence		Length	Mass (Da)
	Isoform p59-HCK [UniParc]. Last modified January 23, 2007. Version 4. Checksum: DF72FD69B38C9706	FASTA	524	59,129
	10 20 30 40 50 60 MGGRSSCEDP GCPRSEGRAP RMGCVKSRFL RDGSKASKTE PSANQKGPVY VPDPTSSSKL 70 80 90 100 110 120 GPNNSNSMPP GFVEGSEDTI VVALYDYEAI HREDLSFQKG DQMVVLEEAG EWWKARSLAT 130 140 150 160 170 180 KKEGYIPSNY VARVNSLETE EWFFKGISRK DAERHLLAPG NMLGSFMIRD SETTKGSYSL 190 200 210 220 230 240 SVRDFDPQHG DTVKHYKIRT LDSGGFYISP RSTFSSLQEL VLHYKKGKDG LCQKLSVPCV 250 260 270 280 290 300 SPKPQKPWEK DAWEIPRESL QMEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF 310 320 330 340 350 360 LAEANLMKSL QHDKLVKLHA VVSQEPIFIV TEFMAKGSLL DFLKSEEGSK QPLPKLIDFS 370 380 390 400 410 420 AQISEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK 430 440 450 460 470 480 WTAPEAINFG SFTIKSDVWS FGILLMEIVT YGRIPYPGMS NPEVIRALEH GYRMPRPDNC 490 500 510 520 PEELYNIMIR CWKNRPEERP TFEYIQSVLD DFYTATESQY QQQP
	Isoform p56-HCK. Checksum: 29E004E0ABA7DF40	FASTA	503	56,942
	10 20 30 40 50 60 MGCVKSRFLR DGSKASKTEP SANQKGPVYV PDPTSSSKLG PNNSNSMPPG FVEGSEDTIV 70 80 90 100 110 120 VALYDYEAIH REDLSFQKGD QMVVLEEAGE WWKARSLATK KEGYIPSNYV ARVNSLETEE 130 140 150 160 170 180 WFFKGISRKD AERHLLAPGN MLGSFMIRDS ETTKGSYSLS VRDFDPQHGD TVKHYKIRTL 190 200 210 220 230 240 DSGGFYISPR STFSSLQELV LHYKKGKDGL CQKLSVPCVS PKPQKPWEKD AWEIPRESLQ 250 260 270 280 290 300 MEKKLGAGQF GEVWMATYNK HTKVAVKTMK PGSMSVEAFL AEANLMKSLQ HDKLVKLHAV 310 320 330 340 350 360 VSQEPIFIVT EFMAKGSLLD FLKSEEGSKQ PLPKLIDFSA QISEGMAFIE QRNYIHRDLR 370 380 390 400 410 420 AANILVSASL VCKIADFGLA RIIEDNEYTA REGAKFPIKW TAPEAINFGS FTIKSDVWSF 430 440 450 460 470 480 GILLMEIVTY GRIPYPGMSN PEVIRALEHG YRMPRPDNCP EELYNIMIRC WKNRPEERPT 490 500 FEYIQSVLDD FYTATESQYQ QQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the mouse hck gene." Klemsz M.J., McKercher S.R., Maki R.A. Nucleic Acids Res. 15:9600-9600(1987) [PubMed: 3684607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM P59-HCK). Strain: ICR. Tissue: Macrophage.
[2]	"Isolation and sequence of a cDNA corresponding to a src-related gene expressed in murine hemopoietic cells." Holtzman D.A., Cook W.D., Dunn A.R. Proc. Natl. Acad. Sci. U.S.A. 84:8325-8329(1987) [PubMed: 3317404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM P59-HCK). Tissue: Macrophage.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM P59-HCK). Strain: C57BL/6J and NOD. Tissue: Bone marrow macrophage and Spleen.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM P59-HCK). Strain: FVB/N. Tissue: Mammary gland.
[5]	"Two isoforms of murine hck, generated by utilization of alternative translational initiation codons, exhibit different patterns of subcellular localization." Lock P., Ralph S., Stanley E., Boulet I., Ramsay R., Dunn A.R. Mol. Cell. Biol. 11:4363-4370(1991) [PubMed: 1875927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-21 (ISOFORM P59-HCK), SUBCELLULAR LOCATION, ALTERNATIVE INITIATION.
[6]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-409, MASS SPECTROMETRY. Tissue: Brain.
[7]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; TYR-207 AND TYR-409, MASS SPECTROMETRY. Tissue: Macrophage.
[8]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-409, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00487 mRNA. Translation: CAA68544.1. Different initiation. J03023 mRNA. Translation: AAA37305.1. Different initiation. AK149736 mRNA. Translation: BAE29054.1. Different initiation. AK150290 mRNA. Translation: BAE29445.1. Different initiation. AK150709 mRNA. Translation: BAE29787.1. Different initiation. AK155975 mRNA. Translation: BAE33532.1. Different initiation. AK165315 mRNA. Translation: BAE38133.1. Different initiation. BC010478 mRNA. Translation: AAH10478.2.
IPI	IPI00129487. IPI00760068.
PIR	TVMSHC. A27282.
RefSeq	NP_034537.2.
UniGene	Mm.715
3D structure databases
HSSP	HSSP built from PDB template 1AD5 based on UniProtKB P08631.
SMR	P08103. Positions 80-524.
ModBase	Search...
PTM databases
PhosphoSite	P08103.
Proteomic databases
PRIDE	P08103.
Genome annotation databases
Ensembl	ENSMUSG00000003283. Mus musculus. [Contig view]
GeneID	15162.
KEGG	mmu:15162.
Organism-specific databases
MGI	MGI:96052. Hck.
Phylogenomic databases
HOGENOM	P08103.
HOVERGEN	P08103.
Enzyme and pathway databases
BRENDA	2.7.10.2. 244.
Gene expression databases
ArrayExpress	P08103.
Bgee	P08103.
CleanEx	MM_HCK.
GermOnline	ENSMUSG00000003283. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	287656.
SOURCE	Search...

Entry information

Entry name

HCK_MOUSE

Accession

Primary (citable) accession number: P08103
Secondary accession number(s): Q0VH03, Q3UD17

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 110 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)