Low affinity immunoglobulin gamma Fc region receptor II precursor

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P08101 (FCGR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 132. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Low affinity immunoglobulin gamma Fc region receptor II

Alternative name(s):
Fc gamma receptor IIB
Short name=Fc-gamma RII
Short name=Fc-gamma-RIIB
Short name=FcRII
IgG Fc receptor II beta
Lymphocyte antigen 17
Short name=Ly-17
CD_antigen=CD32

Gene names

Name:	Fcgr2
Synonyms:	Fcgr2b, Ly-17

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
Subunit structure	Interacts with FGR By similarity. Interacts with LYN. Ref.10
Subcellular location	Cell membrane; Single-pass type I membrane protein. Isoform IIB1: Cytoplasm › cytoskeleton. Note: Binds the cytoskeleton and is not localized in endocytotic pits. Isoform IIB3: Secreted. Note: Released as a soluble molecule.
Tissue specificity	Widely expressed by cells of hemopoietic origin. The isoforms are differentially expressed. Isoform IIB1 is preferentially expressed by cells of the lymphoid lineage, isoform IIB2 by cells of the myeloid lineage, and isoform IIB3 is released by macrophages and is present in the serum. Isoform IIB1' is expressed in myeloid and lymphoid cell lines, in normal spleen cells, and in resting or LPS-activated B-cells but is not detected in mesenteric lymph node cells.
Domain	Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. Another tyrosine-containing sequence, more C-terminal, accounts for the ability of isoform IIB2 to trigger the phagocytosis of particulate immuno complexes.
Post-translational modification	Glycosylated. When coaggregated to BCR, isoform IIB1 and isoform IIB1' become tyrosine phosphorylated and bind to the SH2 domains of the protein tyrosine phosphatase PTPC1. Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN and SYK By similarity. Ref.10
Polymorphism	Ly-17 alloantigenic system involves residues 116 and 161. Ly-17.1 mice are Pro-116 and Glu-161; Ly-17.2 mice are Leu-116 and Leu-161. These polymorphisms do not affect IgG binding.
Sequence similarities	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Sequence caution	The sequence CAA28309.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Ligand	IgG-binding protein
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II Inferred from mutant phenotype PubMed 14643301. Source: MGI cell surface receptor signaling pathway Inferred from mutant phenotype PubMed 8552190. Source: MGI cellular response to molecule of bacterial origin Inferred from mutant phenotype PubMed 12385742. Source: MGI defense response Inferred from mutant phenotype PubMed 11994421. Source: MGI immunoglobulin mediated immune response Inferred from mutant phenotype PubMed 10395649 PubMed 14643301. Source: MGI negative regulation of B cell proliferation Inferred from mutant phenotype PubMed 12385742 PubMed 8552190. Source: MGI negative regulation of humoral immune response mediated by circulating immunoglobulin Inferred from mutant phenotype PubMed 12385742. Source: MGI negative regulation of immunoglobulin production Inferred from mutant phenotype PubMed 12385742. Source: MGI negative regulation of interleukin-10 production Inferred from mutant phenotype PubMed 12385742. Source: MGI negative regulation of phagocytosis Inferred from mutant phenotype PubMed 12385742. Source: MGI negative regulation of type I hypersensitivity Inferred from mutant phenotype PubMed 8552190. Source: MGI phagocytosis, engulfment Inferred from direct assay PubMed 8769481. Source: MGI positive regulation of phagocytosis Inferred from direct assay PubMed 8769481. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell external side of plasma membrane Inferred from direct assay PubMed 8552190 PubMed 8769481. Source: MGI extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred by curator PubMed 8552190. Source: MGI
Molecular_function	IgG binding Inferred from direct assay PubMed 9551950. Source: MGI receptor signaling protein activity Traceable author statement PubMed 11994421. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform IIB1 (identifier: P08101-1) Also known as: Beta-1; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform IIB2 (identifier: P08101-2) Also known as: Beta-2; The sequence of this isoform differs from the canonical sequence as follows: 243-290: ALPGNPDHREMGETLPEEVGEYRQPSGGSVPVSPGPPSGLEPTSSSPY → D

Isoform IIB1' (identifier: P08101-3) Also known as: Beta-1'; The sequence of this isoform differs from the canonical sequence as follows: 262-290: GEYRQPSGGSVPVSPGPPSGLEPTSSSPY → D

Isoform IIB3 (identifier: P08101-4) Also known as: Beta-3; The sequence of this isoform is not available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

Ref.1

Chain

30 – 330

301

Low affinity immunoglobulin gamma Fc region receptor II

PRO_0000015143

Regions

Topological domain

30 – 210

181

Extracellular Potential

Transmembrane

211 – 231

Helical; Potential

Topological domain

232 – 330

Cytoplasmic Potential

Domain

50 – 106

Ig-like C2-type 1

Domain

131 – 189

Ig-like C2-type 2

Motif

307 – 312

ITIM motif

Amino acid modifications

Modified residue

309

Phosphotyrosine; by SRC-type Tyr-kinases By similarity

Modified residue

326

Phosphotyrosine Ref.11

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

166

N-linked (GlcNAc...) Potential

Glycosylation

173

N-linked (GlcNAc...) Potential

Disulfide bond

57 ↔ 99

By similarity

Disulfide bond

138 ↔ 182

By similarity

Natural variations

Alternative sequence

243 – 290

ALPGN…SSSPY → D in isoform IIB2.

VSP_002640

Alternative sequence

262 – 290

GEYRQ…SSSPY → D in isoform IIB1'.

VSP_002641

Natural variant

116

S → L in strain: DBA/2; Ly17.2 allotype.

Natural variant

116

S → P in strain: NZB; Ly17.1 allotype.

Natural variant

145

L → P.

Natural variant

161

H → L in strain: DBA/2; Ly17.2 allotype.

Natural variant

161

H → Q in strain: NZB; Ly17.1 allotype.

Natural variant

190

L → Q.

Natural variant

195

P → T in strain: NZB.

Natural variant

287

S → I in strain: NZB.

Experimental info

Sequence conflict

270 – 278

GSVPVSPGP → LSACQPRA in AAA37608. Ref.1

Sequence conflict

299

A → P Ref.3

Sequence conflict

299

A → P Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform IIB1 (Beta-1) [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A463B9EFF2717511
Show »

FASTA

330

36,695

        10         20         30         40         50         60 
MESNWTVHVF SRTLCHMLLW TAVLNLAAGT HDLPKAVVKL EPPWIQVLKE DTVTLTCEGT 

        70         80         90        100        110        120 
HNPGNSSTQW FHNGRSIRSQ VQASYTFKAT VNDSGEYRCQ MEQTRLSDPV DLGVISDWLL 

       130        140        150        160        170        180 
LQTPQLVFLE GETITLRCHS WRNKLLNRIS FFHNEKSVRY HHYSSNFSIP KANHSHSGDY 

       190        200        210        220        230        240 
YCKGSLGRTL HQSKPVTITV QGPKSSRSLP VLTIVAAVTG IAVAAIVIIL VSLVYLKKKQ 

       250        260        270        280        290        300 
VPALPGNPDH REMGETLPEE VGEYRQPSGG SVPVSPGPPS GLEPTSSSPY NPPDLEEAAK 

       310        320        330 
TEAENTITYS LLKHPEALDE ETEHDYQNHI

« Hide

Isoform IIB2 (Beta-2) [UniParc].

Checksum: 84718A2D4D82EC89
Show »

FASTA

283

31,881

Isoform IIB1' (Beta-1') [UniParc].

Checksum: 7706C4ADE2EF2CB9
Show »

FASTA

302

33,954

Isoform IIB3 (Beta-3) (Sequence not available).

–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors." Ravetch J.V., Luster A.D., Weinshank R., Kochan J., Pavlovec A., Portnoy D.A., Hulmes J., Pan Y.-C.E., Unkeless J.C. Science 234:718-725(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-51 (ISOFORMS IIB1 AND IIB2).
[2]	"A complementary DNA clone for a macrophage-lymphocyte Fc receptor." Lewis V.A., Koch T., Plutner H., Mellman I. Nature 324:372-375(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB2). Tissue: Macrophage.
[3]	"The mouse Fc receptor for IgG (Ly-17): molecular cloning and specificity." Hogarth P.M., Hibbs M.L., Bonadonna L., Scott B.M., Witort E., Pietersz G.A., McKenzie I.F.C. Immunogenetics 26:161-168(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE (ISOFORM IIB1).
[4]	"Identification of the mouse beta Fc gamma RII polymorphism by direct sequencing of amplified genomic DNA." Lah M., Quelch K., Deacon N.J., McKenzie I.F., Hogarth P.M. Immunogenetics 31:202-206(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM IIB1), VARIANTS. Strain: BALB/c. Tissue: Spleen.
[5]	"Structure of the mouse beta Fc gamma receptor II gene." Hogarth P.M., Witort E., Hulett M.D., Bonnerot C., Even J., Fridman W.H., McKenzie I.F.C. J. Immunol. 146:369-376(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS IIB1 AND IIB2).
[6]	"Identification, molecular cloning, biologic properties, and tissue distribution of a novel isoform of murine low-affinity IgG receptor homologous to human Fc gamma RIIB1." Latour S., Fridman W.H., Daeron M. J. Immunol. 157:189-197(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB1'). Strain: DBA/2. Tissue: Mast cell.
[7]	"Nonsynonymous mutations in an Fc-receptor structural gene in NZB mice." Sawchuk D.J., Mahmoudi M., Cairns E., Sinclair N.R.S. Immunogenetics 43:112-113(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-330 (ISOFORMS IIB1 AND IIB2). Strain: DBA/2 and NZB. Tissue: Spleen.
[8]	"The murine Fc receptor for immunoglobulin: purification, partial amino acid sequence, and isolation of cDNA clones." Hibbs M.L., Walker I.D., Kirszbaum L., Peitersz G.A., Deacon N.J., Chambers G.W., McKenzie I.F.C., Hogarth P.M. Proc. Natl. Acad. Sci. U.S.A. 83:6980-6984(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-45.
[9]	"Identification, in mouse macrophages and in serum, of a soluble receptor for the Fc portion of IgG (Fc gamma R) encoded by an alternatively spliced transcript of the Fc gamma RII gene." Tartour E., de la Salle H., de la Salle C., Teillaud C., Camoin L., Galinha A., Latour S., Hanau D., Fridman W.H., Sautes C. Int. Immunol. 5:859-868(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF ISOFORM IIB3. Tissue: Macrophage.
[10]	"Fc epsilon receptor I-associated lyn-dependent phosphorylation of Fc gamma receptor IIB during negative regulation of mast cell activation." Malbec O., Fong D.C., Turner M., Tybulewicz V.L., Cambier J.C., Fridman W.H., Daeron M. J. Immunol. 160:1647-1658(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, INTERACTION WITH LYN.
[11]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-326, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16367 mRNA. Translation: AAA37608.1. M14216 mRNA. Translation: AAA37609.1. M17515 mRNA. Translation: AAA37607.1. M31312 Genomic DNA. Translation: AAA37610.1. X04648 mRNA. Translation: CAA28309.1. Different initiation. U31801 mRNA. Translation: AAA92707.1. U31802 mRNA. Translation: AAA92708.1. U31803 mRNA. Translation: AAA92709.1. U31804 mRNA. Translation: AAA92710.1. M14276 mRNA. Translation: AAA37605.1. U51629 mRNA. Translation: AAA97464.1.
IPI	IPI00129485. IPI00223490. IPI00762704.
PIR	A40071. FCMSG1. B40071. I49660.
RefSeq	NP_034317.1. NM_010187.2.
UniGene	Mm.425062.
3D structure databases
ProteinModelPortal	P08101.
SMR	P08101. Positions 34-203.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000027966.
Protein family/group databases
MEROPS	I43.001.
PTM databases
GlycoSuiteDB	P08101.
PhosphoSite	P08101.
Proteomic databases
PaxDb	P08101.
PRIDE	P08101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	14130.
KEGG	mmu:14130.
UCSC	uc007dmy.2. mouse.
Organism-specific databases
CTD	2213.
MGI	MGI:95499. Fcgr2b.
Phylogenomic databases
eggNOG	NOG25177.
HOVERGEN	HBG051602.
InParanoid	P08101.
KO	K12560.
OrthoDB	EOG4ZS94D.
Gene expression databases
ArrayExpress	P08101.
Bgee	P08101.
CleanEx	MM_FCGR2B.
Genevestigator	P08101.
GermOnline	ENSMUSG00000026656. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. [Graphical view]
SMART	SM00409. IG. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	285226.
SOURCE	Search...

Entry information

Entry name

FCGR2_MOUSE

Accession

Primary (citable) accession number: P08101
Secondary accession number(s): P08102

Q61558

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 132 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents