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Protein

Opsin Rh2

Gene

Rh2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.

Absorptioni

Abs(max)=420 nm1 Publication

GO - Molecular functioni

  • G-protein coupled photoreceptor activity Source: FlyBase

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: FlyBase
  • phototransduction Source: FlyBase
  • protein-chromophore linkage Source: UniProtKB-KW
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Chromophore

Enzyme and pathway databases

ReactomeiR-DME-2187335. The retinoid cycle in cones (daylight vision).
R-DME-2453902. The canonical retinoid cycle in rods (twilight vision).
R-DME-2485179. Activation of the phototransduction cascade.
R-DME-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-DME-419771. Opsins.
R-DME-5620916. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Opsin Rh2
Alternative name(s):
Ocellar opsin
Gene namesi
Name:Rh2
ORF Names:CG16740
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003248. Rh2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 56ExtracellularAdd BLAST56
Transmembranei57 – 81Helical; Name=1Sequence analysisAdd BLAST25
Topological domaini82 – 93CytoplasmicAdd BLAST12
Transmembranei94 – 119Helical; Name=2Sequence analysisAdd BLAST26
Topological domaini120 – 133ExtracellularAdd BLAST14
Transmembranei134 – 153Helical; Name=3Sequence analysisAdd BLAST20
Topological domaini154 – 172CytoplasmicAdd BLAST19
Transmembranei173 – 196Helical; Name=4Sequence analysisAdd BLAST24
Topological domaini197 – 220ExtracellularAdd BLAST24
Transmembranei221 – 248Helical; Name=5Sequence analysisAdd BLAST28
Topological domaini249 – 283CytoplasmicAdd BLAST35
Transmembranei284 – 307Helical; Name=6Sequence analysisAdd BLAST24
Topological domaini308 – 314Extracellular7
Transmembranei315 – 339Helical; Name=7Sequence analysisAdd BLAST25
Topological domaini340 – 381CytoplasmicAdd BLAST42

GO - Cellular componenti

  • integral component of membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001976251 – 381Opsin Rh2Add BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...)Curated1
Disulfide bondi130 ↔ 207PROSITE-ProRule annotation
Modified residuei326N6-(retinylidene)lysine1

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08099.
PRIDEiP08099.

Expressioni

Tissue specificityi

Predominant opsin expressed in the dorsal ocelli.

Gene expression databases

BgeeiFBgn0003248.
ExpressionAtlasiP08099. baseline.
GenevisibleiP08099. DM.

Interactioni

Protein-protein interaction databases

BioGridi67266. 3 interactors.
DIPiDIP-22447N.
IntActiP08099. 1 interactor.
MINTiMINT-844888.
STRINGi7227.FBpp0083111.

Structurei

3D structure databases

ProteinModelPortaliP08099.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
InParanoidiP08099.
KOiK04255.
OMAiWTVMPLV.
OrthoDBiEOG091G0ALC.
PhylomeDBiP08099.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR001735. Opsin_RH1/RH2.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00576. OPSINRH1RH2.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSHLPETP FDLAHSGPRF QAQSSGNGSV LDNVLPDMAH LVNPYWSRFA
60 70 80 90 100
PMDPMMSKIL GLFTLAIMII SCCGNGVVVY IFGGTKSLRT PANLLVLNLA
110 120 130 140 150
FSDFCMMASQ SPVMIINFYY ETWVLGPLWC DIYAGCGSLF GCVSIWSMCM
160 170 180 190 200
IAFDRYNVIV KGINGTPMTI KTSIMKILFI WMMAVFWTVM PLIGWSAYVP
210 220 230 240 250
EGNLTACSID YMTRMWNPRS YLITYSLFVY YTPLFLICYS YWFIIAAVAA
260 270 280 290 300
HEKAMREQAK KMNVKSLRSS EDCDKSAEGK LAKVALTTIS LWFMAWTPYL
310 320 330 340 350
VICYFGLFKI DGLTPLTTIW GATFAKTSAV YNPIVYGISH PKYRIVLKEK
360 370 380
CPMCVFGNTD EPKPDAPASD TETTSEADSK A
Length:381
Mass (Da):42,722
Last modified:August 1, 1988 - v1
Checksum:i628322D228396F9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12896 Genomic DNA. Translation: AAA28734.1.
AE014297 Genomic DNA. Translation: AAF55601.1.
PIRiA24058. OOFF2.
RefSeqiNP_524398.1. NM_079674.3.
UniGeneiDm.2404.

Genome annotation databases

EnsemblMetazoaiFBtr0083697; FBpp0083111; FBgn0003248.
GeneIDi42261.
KEGGidme:Dmel_CG16740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12896 Genomic DNA. Translation: AAA28734.1.
AE014297 Genomic DNA. Translation: AAF55601.1.
PIRiA24058. OOFF2.
RefSeqiNP_524398.1. NM_079674.3.
UniGeneiDm.2404.

3D structure databases

ProteinModelPortaliP08099.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67266. 3 interactors.
DIPiDIP-22447N.
IntActiP08099. 1 interactor.
MINTiMINT-844888.
STRINGi7227.FBpp0083111.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP08099.
PRIDEiP08099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083697; FBpp0083111; FBgn0003248.
GeneIDi42261.
KEGGidme:Dmel_CG16740.

Organism-specific databases

CTDi42261.
FlyBaseiFBgn0003248. Rh2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
InParanoidiP08099.
KOiK04255.
OMAiWTVMPLV.
OrthoDBiEOG091G0ALC.
PhylomeDBiP08099.

Enzyme and pathway databases

ReactomeiR-DME-2187335. The retinoid cycle in cones (daylight vision).
R-DME-2453902. The canonical retinoid cycle in rods (twilight vision).
R-DME-2485179. Activation of the phototransduction cascade.
R-DME-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-DME-419771. Opsins.
R-DME-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

ChiTaRSiRh2. fly.
GenomeRNAii42261.
PROiP08099.

Gene expression databases

BgeeiFBgn0003248.
ExpressionAtlasiP08099. baseline.
GenevisibleiP08099. DM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR001735. Opsin_RH1/RH2.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00576. OPSINRH1RH2.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPS2_DROME
AccessioniPrimary (citable) accession number: P08099
Secondary accession number(s): Q9VE29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.