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Protein

Negative regulator of sexual conjugation and meiosis

Gene

ran1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a negative regulator of both sexual conjugation and meiosis. It phosphorylates mei2. It blocks the onset of meiosis until conjugation takes place.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47ATPPROSITE-ProRule annotation1
Active sitei143Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  • conjugation Source: UniProtKB-KW
  • intracellular signal transduction Source: GO_Central
  • meiotic cell cycle Source: UniProtKB-KW
  • negative regulation of induction of conjugation with cellular fusion Source: PomBase
  • negative regulation of meiotic cell cycle Source: PomBase
  • negative regulation of signal transduction involved in conjugation with cellular fusion Source: PomBase
  • negative regulation of transcription factor import into nucleus Source: PomBase
  • negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  • protein autophosphorylation Source: PomBase
  • protein phosphorylation Source: PomBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processConjugation, Meiosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of sexual conjugation and meiosis (EC:2.7.11.1)
Gene namesi
Name:ran1
Synonyms:pat1
ORF Names:SPBC19C2.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.05.
PomBaseiSPBC19C2.05. ran1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866021 – 470Negative regulator of sexual conjugation and meiosisAdd BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei469Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP08092.

PTM databases

iPTMnetiP08092.

Interactioni

Protein-protein interaction databases

BioGridi277106. 58 interactors.
DIPiDIP-10N.
MINTiMINT-4686990.

Structurei

3D structure databases

ProteinModelPortaliP08092.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 295Protein kinasePROSITE-ProRule annotationAdd BLAST278

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi319 – 326Poly-Gln8
Compositional biasi404 – 410Poly-Ala7

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000197083.
InParanoidiP08092.
OrthoDBiEOG092C2EK7.
PhylomeDBiP08092.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P08092-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRENPELLL GQVLGDSLRF VSIIGAGAYG VVYKAEDIYD GTLYAVKALC
60 70 80 90 100
KDGLNEKQKK LQARELALHA RVSSHPYIIT LHRVLETEDA IYVVLQYCPN
110 120 130 140 150
GDLFTYITEK KVYQGNSHLI KTVFLQLISA VEHCHSVGIY HRDLKPENIM
160 170 180 190 200
VGNDVNTVYL ADFGLATTEP YSSDFGCGSL FYMSPECQRE VKKLSSLSDM
210 220 230 240 250
LPVTPEPIES QSSSFATAPN DVWALGIILI NLCCKRNPWK RACSQTDGTY
260 270 280 290 300
RSYVHNPSTL LSILPISREL NSLLNRIFDR NPKTRITLPE LSTLVSNCKN
310 320 330 340 350
LTRRLRPAPL VSSRYLAYQQ QQQQQQMNLQ QGIQGYPHQG YMPTQNIGFP
360 370 380 390 400
WPPTPQFVSN WNHCATPTIP VSLQVLTPNS SLKVDPTTPL TAPIHATESF
410 420 430 440 450
WPSAAAAAAA VHNNANSYMP ITPTPYPNNA KIFGYPNQPP LTPIPFTGFV
460 470
LHPAPVGRAA DAVDPSRKSL
Length:470
Mass (Da):52,212
Last modified:April 27, 2001 - v2
Checksum:iD42143353ACC7828
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155V → G in CAA28437 (PubMed:3830131).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04728 Genomic DNA. Translation: CAA28437.1.
CU329671 Genomic DNA. Translation: CAB52032.1.
PIRiA25685.
T39796.
RefSeqiNP_595690.1. NM_001021587.2.

Genome annotation databases

EnsemblFungiiSPBC19C2.05.1; SPBC19C2.05.1:pep; SPBC19C2.05.
GeneIDi2540580.
KEGGispo:SPBC19C2.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04728 Genomic DNA. Translation: CAA28437.1.
CU329671 Genomic DNA. Translation: CAB52032.1.
PIRiA25685.
T39796.
RefSeqiNP_595690.1. NM_001021587.2.

3D structure databases

ProteinModelPortaliP08092.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277106. 58 interactors.
DIPiDIP-10N.
MINTiMINT-4686990.

PTM databases

iPTMnetiP08092.

Proteomic databases

PRIDEiP08092.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC19C2.05.1; SPBC19C2.05.1:pep; SPBC19C2.05.
GeneIDi2540580.
KEGGispo:SPBC19C2.05.

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.05.
PomBaseiSPBC19C2.05. ran1.

Phylogenomic databases

HOGENOMiHOG000197083.
InParanoidiP08092.
OrthoDBiEOG092C2EK7.
PhylomeDBiP08092.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.

Miscellaneous databases

PROiPR:P08092.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRAN1_SCHPO
AccessioniPrimary (citable) accession number: P08092
Secondary accession number(s): Q9UUD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 27, 2001
Last modified: March 15, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.