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P08091 (PPA1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acid phosphatase

EC=3.1.3.2
Gene names
Name:pho1
ORF Names:SPBP4G3.02
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Secretedcell wall.

Induction

Repressed by phosphate and weakly by thiamine.

Sequence similarities

Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
   Cellular componentCell wall
Secreted
   DomainSignal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to phosphate starvation

Inferred from mutant phenotype PubMed 7499352. Source: PomBase

dephosphorylation

Inferred from mutant phenotype Ref.1PubMed 4092931. Source: PomBase

organophosphate catabolic process

Inferred from mutant phenotype Ref.1. Source: PomBase

positive regulation of L-glutamate import

Inferred from direct assay PubMed 3447747. Source: PomBase

positive regulation of L-glutamine import

Inferred from mutant phenotype PubMed 3447747. Source: PomBase

positive regulation of L-threonine import

Inferred from mutant phenotype PubMed 3447747. Source: PomBase

positive regulation of L-tyrosine import

Inferred from mutant phenotype PubMed 3447747. Source: PomBase

positive regulation of glycine import

Inferred from direct assay PubMed 3447747. Source: PomBase

   Cellular_componentcell wall-bounded periplasmic space

Inferred from direct assay PubMed 2381421. Source: PomBase

cytoplasm

Inferred from mutant phenotype Ref.1. Source: PomBase

endoplasmic reticulum

Inferred from direct assay PubMed 16823372. Source: PomBase

external side of plasma membrane

Non-traceable author statement. Source: PomBase

extracellular region

Inferred from direct assay PubMed 2381421. Source: PomBase

fungal-type cell wall

Inferred from sequence orthology. Source: PomBase

intracellular

Inferred from direct assay PubMed 2381421PubMed 3732265. Source: PomBase

membrane

Inferred from direct assay PubMed 4092931. Source: PomBase

plasma membrane

Inferred from direct assay PubMed 3732265. Source: PomBase

   Molecular_functionacid phosphatase activity

Inferred from mutant phenotype PubMed 1394510Ref.1. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Chain19 – 453435Acid phosphatase
PRO_0000023951

Sites

Active site691Nucleophile By similarity
Active site3311Proton donor By similarity

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation1831N-linked (GlcNAc...) Potential
Glycosylation1931N-linked (GlcNAc...) Potential
Glycosylation2431N-linked (GlcNAc...) Potential
Glycosylation3191N-linked (GlcNAc...) Potential
Glycosylation4101N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Glycosylation4431N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P08091 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 7CF891256EB154D1

FASTA45350,557
        10         20         30         40         50         60 
MFLQNLFLGF LAVVCANAQF AEFTAFDGKF DFKEHLTSRS PYHKPYFYGP SIDFPTTCKI 

        70         80         90        100        110        120 
KQVHTLQRHG SRNPTGGNAA FDAVGIANFQ QRLLNGSVPI DYSVSGNPLS FVPTWTPVIE 

       130        140        150        160        170        180 
AANADALSSS GRVELFDMGR QFYERYHELF NASTYNIYTA AQQRVVDSAL WYGYGMFGED 

       190        200        210        220        230        240 
VHNFTNYILV SENATAGSNS LSSYNACPAS DADDFTTPAL EAWRNVYMPP IRQRLNPYFS 

       250        260        270        280        290        300 
NYNLTNDDIL NLYGICSYEI ALQDYSEFCK LFNSVDFLNF EYEGDLSFSY GMGNSVKWGS 

       310        320        330        340        350        360 
IFGGAYANSL ANSLRSVENN TQQVFFAFTH DANIIPVETA LGFFTDNTPE NPLPTSYQVH 

       370        380        390        400        410        420 
SHSMKASEFV PFAGNLITEL FQCEDSKYYV RHLVNEEVFP LSDCGFGPSN TSDGMCELYA 

       430        440        450 
YLNSPVRVNG TSNGIQNFDT LCNASAVAAV YPY 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the structural gene for secreted acid phosphatase from Schizosaccharomyces pombe."
Elliott S., Chang C., Schweingruber M.E., Schaller J., Rickli E.E., Carbon J.
J. Biol. Chem. 261:2936-2941(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 88-285.
Strain: ATCC 38364 / 968.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11857 Genomic DNA. Translation: AAA35321.1.
CU329671 Genomic DNA. Translation: CAB68657.1.
AB027949 Genomic DNA. Translation: BAA87253.1.
PIRA25326.
RefSeqNP_596847.1. NM_001023870.2.

3D structure databases

ProteinModelPortalP08091.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277850. 17 interactions.
MINTMINT-4686981.
STRING4896.SPBP4G3.02-1.

Proteomic databases

MaxQBP08091.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBP4G3.02.1; SPBP4G3.02.1:pep; SPBP4G3.02.
GeneID2541339.
KEGGspo:SPBP4G3.02.

Organism-specific databases

PomBaseSPBP4G3.02.

Phylogenomic databases

HOGENOMHOG000115655.
KOK01078.
OrthoDBEOG7SN8N6.
PhylomeDBP08091.

Family and domain databases

Gene3D3.40.50.1240. 1 hit.
InterProIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMSSF53254. SSF53254. 1 hit.
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20802448.

Entry information

Entry namePPA1_SCHPO
AccessionPrimary (citable) accession number: P08091
Secondary accession number(s): Q9UTX1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names