ID   BNZE_PSEPU              Reviewed;         275 AA.
AC   P08088;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   16-JUN-2009, entry version 60.
DE   RecName: Full=Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase;
DE            EC=1.3.1.19;
DE   AltName: Full=Cis-benzene glycol dehydrogenase;
GN   Name=bnzE;
OS   Pseudomonas putida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BE-81;
RX   MEDLINE=88032840; PubMed=3667527;
RA   Irie S., Doi S., Yorifuji T., Takagi M., Yano K.;
RT   "Nucleotide sequencing and characterization of the genes encoding
RT   benzene oxidation enzymes of Pseudomonas putida.";
RL   J. Bacteriol. 169:5174-5179(1987).
CC   -!- CATALYTIC ACTIVITY: Cis-1,2-dihydrobenzene-1,2-diol + NAD(+) =
CC       catechol + NADH.
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation;
CC       catechol from benzene: step 2/2.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; M17904; AAA25739.1; ALT_INIT; Genomic_DNA.
DR   PIR; E36516; E36516.
DR   HSSP; P47227; 1BDB.
DR   BRENDA; 1.3.1.19; 403.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0018504; F:cis-1,2-dihydrobenzene-1,2-diol dehydrogena...; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017711; BphB_TodD.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   TIGRFAMs; TIGR03325; BphB_TodD; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN         1    275       Cis-1,2-dihydrobenzene-1,2-diol
FT                                dehydrogenase.
FT                                /FTId=PRO_0000054530.
FT   NP_BIND       9     33       NAD (By similarity).
FT   ACT_SITE    155    155       Proton acceptor (By similarity).
FT   BINDING     142    142       Substrate (By similarity).
SQ   SEQUENCE   275 AA;  28782 MW;  D5919208D859234B CRC64;
     MRLEGEVALV TGGGAGLGRA IVDRYVAEGA RVAVLDKSAA GLEALRKLHG DAIVGVEGDV
     RSLDSHREAV ARCVEAFGKL DCLVGNAGVW DYLTQLVDIP DDLISEAFEE MFEVNVKGYI
     LAAKAALPAL YQSKGSAIFT VSNAGFYPGG GGVLYTAGKH AVIGLIKQLA HEWGPRIRVN
     GIAPGGILGS DLRGLKSLDL QDKSISTFPL DDMLKSVLPT GRAATAEEYA GAYVFFATRG
     DTVPLTGSVL NFDGGMGVRG LFEASLGAQL DKHFG
//