Reviewed, UniProtKB/Swiss-Prot P08088 (BNZE_PSEPU)

Names and origin

Protein names

Recommended name:
    Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase
    EC=1.3.1.19
Alternative name(s):
    Cis-benzene glycol dehydrogenase

Gene names

Name:

bnzE

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cis-1,2-dihydrobenzene-1,2-diol + NAD+ = catechol + NADH.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 2/2.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro cis-1,2-dihydrobenzene-1,2-diol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase		PRO_0000054530
Regions
	Nucleotide binding	9 – 33	25	NAD By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08088-1 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: D5919208D859234B	FASTA	275	28,782
	10 20 30 40 50 60 MRLEGEVALV TGGGAGLGRA IVDRYVAEGA RVAVLDKSAA GLEALRKLHG DAIVGVEGDV 70 80 90 100 110 120 RSLDSHREAV ARCVEAFGKL DCLVGNAGVW DYLTQLVDIP DDLISEAFEE MFEVNVKGYI 130 140 150 160 170 180 LAAKAALPAL YQSKGSAIFT VSNAGFYPGG GGVLYTAGKH AVIGLIKQLA HEWGPRIRVN 190 200 210 220 230 240 GIAPGGILGS DLRGLKSLDL QDKSISTFPL DDMLKSVLPT GRAATAEEYA GAYVFFATRG 250 260 270 DTVPLTGSVL NFDGGMGVRG LFEASLGAQL DKHFG

References

[1]	"Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida." Irie S., Doi S., Yorifuji T., Takagi M., Yano K. J. Bacteriol. 169:5174-5179(1987) [PubMed: 3667527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BE-81.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17904 Genomic DNA. Translation: AAA25739.1. Different initiation.
PIR	E36516.
3D structure databases
HSSP	HSSP built from PDB template 1BDB based on UniProtKB P47227.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.19. 403.
Family and domain databases
InterPro	IPR017711. BphB_TodD. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. [Graphical view]
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR03325. BphB_TodD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BNZE_PSEPU

Accession

Primary (citable) accession number: P08088

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)