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Protein

Colicin-N

Gene

cna

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.3.3. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-N
Gene namesi
Name:cna
Encoded oniPlasmid ColN pCHAP40 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei325 – 34521HelicalSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Colicin-NPRO_0000218674Add
BLAST

Proteomic databases

PRIDEiP08083.

Interactioni

Protein-protein interaction databases

DIPiDIP-29645N.

Structurei

Secondary structure

1
387
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi97 – 1015Combined sources
Beta strandi108 – 1125Combined sources
Helixi114 – 1163Combined sources
Turni117 – 1193Combined sources
Beta strandi121 – 1288Combined sources
Beta strandi131 – 1377Combined sources
Beta strandi143 – 1486Combined sources
Helixi149 – 1513Combined sources
Helixi153 – 1553Combined sources
Beta strandi157 – 1626Combined sources
Helixi170 – 21445Combined sources
Helixi216 – 23015Combined sources
Helixi234 – 2363Combined sources
Helixi240 – 25112Combined sources
Helixi260 – 27112Combined sources
Helixi275 – 2839Combined sources
Helixi287 – 2893Combined sources
Helixi294 – 30916Combined sources
Helixi315 – 32612Combined sources
Helixi331 – 34313Combined sources
Helixi352 – 37019Combined sources
Helixi372 – 38514Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A87X-ray3.10A67-387[»]
DisProtiDP00461.
ProteinModelPortaliP08083.
SMRiP08083. Positions 91-387.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08083.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 4949Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSNGADNAH NNAFGGGKNP GIGNTSGAGS NGSASSNRGN SNGWSWSNKP
60 70 80 90 100
HKNDGFHSDG SYHITFHGDN NSKPKPGGNS GNRGNNGDGA SAKVGEITIT
110 120 130 140 150
PDNSKPGRYI SSNPEYSLLA KLIDAESIKG TEVYTFHTRK GQYVKVTVPD
160 170 180 190 200
SNIDKMRVDY VNWKGPKYNN KLVKRFVSQF LLFRKEEKEK NEKEALLKAS
210 220 230 240 250
ELVSGMGDKL GEYLGVKYKN VAKEVANDIK NFHGRNIRSY NEAMASLNKV
260 270 280 290 300
LANPKMKVNK SDKDAIVNAW KQVNAKDMAN KIGNLGKAFK VADLAIKVEK
310 320 330 340 350
IREKSIEGYN TGNWGPLLLE VESWIIGGVV AGVAISLFGA VLSFLPISGL
360 370 380
AVTALGVIGI MTISYLSSFI DANRVSNINN IISSVIR
Length:387
Mass (Da):41,743
Last modified:August 1, 1988 - v1
Checksum:i1C4342E222F8CECD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00533 Genomic DNA. Translation: CAA68592.1.
X06933 Genomic DNA. Translation: CAA30021.1.
PIRiS00867.
RefSeqiWP_032488335.1. NZ_LOPH01000042.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00533 Genomic DNA. Translation: CAA68592.1.
X06933 Genomic DNA. Translation: CAA30021.1.
PIRiS00867.
RefSeqiWP_032488335.1. NZ_LOPH01000042.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A87X-ray3.10A67-387[»]
DisProtiDP00461.
ProteinModelPortaliP08083.
SMRiP08083. Positions 91-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29645N.

Protein family/group databases

TCDBi1.C.1.3.3. the channel-forming colicin (colicin) family.

Proteomic databases

PRIDEiP08083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08083.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequencing of the structural gene for colicin N reveals homology between the catalytic, C-terminal domains of colicins A and N."
    Pugsley A.P.
    Mol. Microbiol. 1:317-325(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The immunity and lysis genes of ColN plasmid pCHAP4."
    Pugsley A.P.
    Mol. Gen. Genet. 211:335-341(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 372-387.
  3. "Crystal structure of a colicin N fragment suggests a model for toxicity."
    Vetter I.R., Parker M.W., Tucker A.D., Lakey J.H., Pattus F., Tsernoglou D.
    Structure 6:863-874(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 91-387.

Entry informationi

Entry nameiCEAN_ECOLX
AccessioniPrimary (citable) accession number: P08083
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 11, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.