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Protein

Colicin-N

Gene

cna

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.3.3. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-N
Gene namesi
Name:cna
Encoded oniPlasmid ColN pCHAP40 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Transmembranei350 – 370HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186741 – 387Colicin-NAdd BLAST387

Interactioni

Protein-protein interaction databases

DIPiDIP-29645N.

Structurei

Secondary structure

1387
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi97 – 101Combined sources5
Beta strandi108 – 112Combined sources5
Helixi114 – 116Combined sources3
Turni117 – 119Combined sources3
Beta strandi121 – 128Combined sources8
Beta strandi131 – 137Combined sources7
Beta strandi143 – 148Combined sources6
Helixi149 – 151Combined sources3
Helixi153 – 155Combined sources3
Beta strandi157 – 162Combined sources6
Helixi170 – 214Combined sources45
Helixi216 – 230Combined sources15
Helixi234 – 236Combined sources3
Helixi240 – 251Combined sources12
Helixi260 – 271Combined sources12
Helixi275 – 283Combined sources9
Helixi287 – 289Combined sources3
Helixi294 – 309Combined sources16
Helixi315 – 326Combined sources12
Helixi331 – 343Combined sources13
Helixi352 – 370Combined sources19
Helixi372 – 385Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A87X-ray3.10A67-387[»]
DisProtiDP00461.
ProteinModelPortaliP08083.
SMRiP08083.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08083.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 49Gly-richAdd BLAST49

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSNGADNAH NNAFGGGKNP GIGNTSGAGS NGSASSNRGN SNGWSWSNKP
60 70 80 90 100
HKNDGFHSDG SYHITFHGDN NSKPKPGGNS GNRGNNGDGA SAKVGEITIT
110 120 130 140 150
PDNSKPGRYI SSNPEYSLLA KLIDAESIKG TEVYTFHTRK GQYVKVTVPD
160 170 180 190 200
SNIDKMRVDY VNWKGPKYNN KLVKRFVSQF LLFRKEEKEK NEKEALLKAS
210 220 230 240 250
ELVSGMGDKL GEYLGVKYKN VAKEVANDIK NFHGRNIRSY NEAMASLNKV
260 270 280 290 300
LANPKMKVNK SDKDAIVNAW KQVNAKDMAN KIGNLGKAFK VADLAIKVEK
310 320 330 340 350
IREKSIEGYN TGNWGPLLLE VESWIIGGVV AGVAISLFGA VLSFLPISGL
360 370 380
AVTALGVIGI MTISYLSSFI DANRVSNINN IISSVIR
Length:387
Mass (Da):41,743
Last modified:August 1, 1988 - v1
Checksum:i1C4342E222F8CECD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00533 Genomic DNA. Translation: CAA68592.1.
X06933 Genomic DNA. Translation: CAA30021.1.
PIRiS00867.
RefSeqiWP_032488335.1. NZ_LOPH01000042.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00533 Genomic DNA. Translation: CAA68592.1.
X06933 Genomic DNA. Translation: CAA30021.1.
PIRiS00867.
RefSeqiWP_032488335.1. NZ_LOPH01000042.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A87X-ray3.10A67-387[»]
DisProtiDP00461.
ProteinModelPortaliP08083.
SMRiP08083.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29645N.

Protein family/group databases

TCDBi1.C.1.3.3. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08083.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEAN_ECOLX
AccessioniPrimary (citable) accession number: P08083
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.