Reviewed, UniProtKB/Swiss-Prot P08080 (HEM1_RHIME)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase

Gene names

Name:	hemA
Ordered Locus Names:	R02989
ORF Names:	SMc03104

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	5-aminolevulinate synthase		PRO_0000163827
Amino acid modifications
	Modified residue	247	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08080-1 [UniParc]. Last modified October 18, 2001. Version 2. Checksum: 826BC15187083E07	FASTA	404	44,116
	10 20 30 40 50 60 MDFESFFKNE LDGLHQEGRY RVFADLARHR GSFPKATRYT ADGAQEVTVW CSNDYLGMGQ 70 80 90 100 110 120 CPIVTEAMKN AIDECGAGAG GTRNISGTNH YHVLLERELA DLHGKESALL FTSGYVSNWA 130 140 150 160 170 180 ALGTLCSKIP GVIVFSDAGN HASMIEGIRH SKCERVIFKH NSVADLEAKL AAADPRAPKI 190 200 210 220 230 240 IAFESVYSMD GDIAPIREFC DLADKYGAMT YLDEVHAVGM YGPRGGGIAE REGLMHRLTV 250 260 270 280 290 300 IEGTLGKAFG VMGGYITGSA ALCDFIRSFA SGFIFTTALP PALAAGALAS IRHLKESQVE 310 320 330 340 350 360 RFAHQERVRR LRSLLDQRGI PHMVNPSHIV PVIVGDAAKC KWISDLLLDN FGIYVQPINY 370 380 390 400 PTVPKKTERL RITPTPMHSD ADIDHLVSAL HSLWSRCALA RAVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[2]	"Analysis of the 5' regulatory region of the gene for delta-aminolevulinic acid synthetase of Rhizobium meliloti." Leong S.A., Williams P.H., Ditta G.S. Nucleic Acids Res. 13:5965-5976(1985) [PubMed: 2994020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC47568.1. Different initiation. X02853 Genomic DNA. Translation: CAA26610.1. Different initiation.
PIR	A24077.
RefSeq	NP_387095.1.
3D structure databases
HSSP	HSSP built from PDB template 1FC4 based on UniProtKB P07912.
ModBase	Search...
Genome annotation databases
GeneID	1234673.
GenomeReviews	Gene locus R02989 in contig AL591688_GR.
KEGG	sme:SMc03104.
NMPDR	fig|266834.1.peg.4283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P08080.
OMA	P08080. FSPIKDI.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03104-MON.
BRENDA	2.3.1.37. 142.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR004839. Aminotrans_I/II. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_RHIME

Accession

Primary (citable) accession number: P08080

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 18, 2001
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)