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P08080 (HEM1_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-aminolevulinate synthase

EC=2.3.1.37
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene names
Name:hemA
Ordered Locus Names:R02989
ORF Names:SMc03104
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactor

Pyridoxal phosphate.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Sequence caution

The sequence CAA26610.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAC47568.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processHeme biosynthesis
   LigandPyridoxal phosphate
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function5-aminolevulinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4044045-aminolevulinate synthase
PRO_0000163827

Sites

Active site2471 By similarity
Binding site211Substrate By similarity
Binding site1361Substrate By similarity
Binding site1881Pyridoxal phosphate By similarity
Binding site2161Pyridoxal phosphate By similarity
Binding site2441Pyridoxal phosphate By similarity
Binding site2761Pyridoxal phosphate By similarity
Binding site2771Pyridoxal phosphate By similarity
Binding site3621Substrate By similarity

Amino acid modifications

Modified residue2471N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P08080 [UniParc].

Last modified October 18, 2001. Version 2.
Checksum: 826BC15187083E07

FASTA40444,116
        10         20         30         40         50         60 
MDFESFFKNE LDGLHQEGRY RVFADLARHR GSFPKATRYT ADGAQEVTVW CSNDYLGMGQ 

        70         80         90        100        110        120 
CPIVTEAMKN AIDECGAGAG GTRNISGTNH YHVLLERELA DLHGKESALL FTSGYVSNWA 

       130        140        150        160        170        180 
ALGTLCSKIP GVIVFSDAGN HASMIEGIRH SKCERVIFKH NSVADLEAKL AAADPRAPKI 

       190        200        210        220        230        240 
IAFESVYSMD GDIAPIREFC DLADKYGAMT YLDEVHAVGM YGPRGGGIAE REGLMHRLTV 

       250        260        270        280        290        300 
IEGTLGKAFG VMGGYITGSA ALCDFIRSFA SGFIFTTALP PALAAGALAS IRHLKESQVE 

       310        320        330        340        350        360 
RFAHQERVRR LRSLLDQRGI PHMVNPSHIV PVIVGDAAKC KWISDLLLDN FGIYVQPINY 

       370        380        390        400 
PTVPKKTERL RITPTPMHSD ADIDHLVSAL HSLWSRCALA RAVA 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. expand/collapse author list , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[2]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"Analysis of the 5' regulatory region of the gene for delta-aminolevulinic acid synthetase of Rhizobium meliloti."
Leong S.A., Williams P.H., Ditta G.S.
Nucleic Acids Res. 13:5965-5976(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591688 Genomic DNA. Translation: CAC47568.1. Different initiation.
X02853 Genomic DNA. Translation: CAA26610.1. Different initiation.
PIRA24077.
RefSeqNP_387095.1. NC_003047.1.

3D structure databases

ProteinModelPortalP08080.
SMRP08080. Positions 2-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc03104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC47568; CAC47568; SMc03104.
GeneID1234673.
KEGGsme:SMc03104.
PATRIC23635586. VBISinMel96828_4516.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0156.
HOGENOMHOG000221020.
KOK00643.
OMAHYLQSIN.
OrthoDBEOG6Q8HZD.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-3931-MONOMER.
RETL1328306-WGS:GSTH-4473-MONOMER.
SMEL266834:GJF6-3065-MONOMER.
UniPathwayUPA00251; UER00375.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR01821. 5aminolev_synth. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM1_RHIME
AccessionPrimary (citable) accession number: P08080
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 18, 2001
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways