ID   STRA_STRGR              Reviewed;         307 AA.
AC   P08077;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   05-MAY-2009, entry version 49.
DE   RecName: Full=Streptomycin 6-kinase;
DE            EC=2.7.1.72;
DE   AltName: Full=Streptidine kinase;
DE   AltName: Full=Streptomycin 6-phosphotransferase;
DE   AltName: Full=APH(6);
GN   Name=aphD; Synonyms=sph, strA;
OS   Streptomyces griseus.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=N2-3-11;
RX   MEDLINE=88040426; PubMed=3118332; DOI=10.1093/nar/15.19.8041;
RA   Distler J., Ebert A., Mansouri K., Pissowotzki K., Stockmann M.,
RA   Piepersberg W.;
RT   "Gene cluster for streptomycin biosynthesis in Streptomyces griseus:
RT   nucleotide sequence of three genes and analysis of transcriptional
RT   activity.";
RL   Nucleic Acids Res. 15:8041-8056(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23345 / DSM 40236 / IFO 12875 / JCM 4644 / NCIMB 13023;
RX   MEDLINE=87146497; PubMed=3029728; DOI=10.1093/nar/15.4.1819;
RA   Tohyama H., Okami Y., Umezawa H.;
RT   "Nucleotide sequence of the streptomycinphosphotransferase and
RT   amidinotransferase genes from Streptomyces griseus.";
RL   Nucleic Acids Res. 15:1819-1833(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=87286403; PubMed=3039306; DOI=10.1007/BF00330443;
RA   Distler J., Braun C., Ebert A., Piepersberg W.;
RT   "Gene cluster for streptomycin biosynthesis in Streptomyces griseus:
RT   analysis of a central region including the major resistance gene.";
RL   Mol. Gen. Genet. 208:204-210(1987).
CC   -!- FUNCTION: The aminoglycoside phosphotransferases achieve
CC       inactivation of their antibiotic substrates by phosphorylation.
CC   -!- CATALYTIC ACTIVITY: ATP + streptomycin = ADP + streptomycin 6-
CC       phosphate.
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase
CC       family.
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DR   EMBL; Y00459; CAA68516.1; -; Genomic_DNA.
DR   EMBL; X05045; CAA28717.1; -; Genomic_DNA.
DR   EMBL; X05647; CAA29134.1; -; Genomic_DNA.
DR   PIR; A26674; A26674.
DR   PIR; S06355; KISM6G.
DR   BioCyc; MetaCyc:MON-14016; -.
DR   BRENDA; 2.7.1.72; 1270.
DR   GO; GO:0050300; F:aminoglycoside 6-kinase activity; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein amino acid phosphorylation; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0019748; P:secondary metabolic process; IEA:InterPro.
DR   InterPro; IPR006748; NH2Glyco/OHUrea_AB-resist_kin.
DR   Pfam; PF04655; APH_6_hur; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Transferase.
FT   CHAIN         1    307       Streptomycin 6-kinase.
FT                                /FTId=PRO_0000204815.
FT   REGION      133    145       Aminoglycoside binding (By similarity).
FT   ACT_SITE    201    201       By similarity.
FT   VARIANT      34     39       ALVARC -> GSRRAI (in strain: ISP 5236).
FT   VARIANT     115    120       DGSRTL -> GRFADV (in strain: ISP 5236).
FT   VARIANT     181    188       GWASAVAE -> AVGVGREP (in strain: ISP
FT                                5236).
FT   VARIANT     225    225       G -> A (in strain: ISP 5236).
FT   VARIANT     262    262       S -> A (in strain: ISP 5236).
SQ   SEQUENCE   307 AA;  33244 MW;  382DFD60DF4028AA CRC64;
     MSSSDHIHVP DGLAESYSRS GGEEGRAWIA GLPALVARCV DRWELKRDGG VRSGEASLVV
     PVLRADGTRA ALKLQMPREE TTAALIGLRA WGGDGMVRLL DHDEESSTML LERLDGSRTL
     ASVEDDDEAM GVLAGLLNRL HSVPAPPGLR GLGEIAGAMV EEVPSAVDSL ADPEDRSRLR
     GWASAVAELV GEPGDRVLHW DLHYENVLAA EREPWLAIDP EPLVGDPGFD LWPALDTGWE
     RIEATGDARR VVRRRFDLLT ESLELDRGRA AGWTLARLLQ NTLWDIEDGL TAIAPSQIAV
     AEALAKP
//