Reviewed, UniProtKB/Swiss-Prot P08077 (STRA_STRGR)

Names and origin

Protein names

Recommended name:
    Streptomycin 6-kinase
    EC=2.7.1.72
Alternative name(s):
    Streptidine kinase
    Streptomycin 6-phosphotransferase
    APH(6)

Gene names

Name:	aphD
Synonyms:	sph, strA

Organism

Streptomyces griseus

Taxonomic identifier

1911 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation.
Catalytic activity	ATP + streptomycin = ADP + streptomycin 6-phosphate.
Sequence similarities	Belongs to the aminoglycoside phosphotransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW secondary metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoglycoside 6-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Streptomycin 6-kinase		PRO_0000204815
Regions
	Region	133 – 145	13	Aminoglycoside binding By similarity
Sites
	Active site	201	1	By similarity
Natural variations
	Natural variant	34 – 39	6	ALVARC → GSRRAI in strain: ISP 5236.
	Natural variant	115 – 120	6	DGSRTL → GRFADV in strain: ISP 5236.
	Natural variant	181 – 188	8	GWASAVAE → AVGVGREP in strain: ISP 5236.
	Natural variant	225	1	G → A in strain: ISP 5236.
	Natural variant	262	1	S → A in strain: ISP 5236.

Sequences

	Sequence		Length	Mass (Da)
	P08077-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 382DFD60DF4028AA	FASTA	307	33,244
	10 20 30 40 50 60 MSSSDHIHVP DGLAESYSRS GGEEGRAWIA GLPALVARCV DRWELKRDGG VRSGEASLVV 70 80 90 100 110 120 PVLRADGTRA ALKLQMPREE TTAALIGLRA WGGDGMVRLL DHDEESSTML LERLDGSRTL 130 140 150 160 170 180 ASVEDDDEAM GVLAGLLNRL HSVPAPPGLR GLGEIAGAMV EEVPSAVDSL ADPEDRSRLR 190 200 210 220 230 240 GWASAVAELV GEPGDRVLHW DLHYENVLAA EREPWLAIDP EPLVGDPGFD LWPALDTGWE 250 260 270 280 290 300 RIEATGDARR VVRRRFDLLT ESLELDRGRA AGWTLARLLQ NTLWDIEDGL TAIAPSQIAV AEALAKP

References

[1]	"Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity." Distler J., Ebert A., Mansouri K., Pissowotzki K., Stockmann M., Piepersberg W. Nucleic Acids Res. 15:8041-8056(1987) [PubMed: 3118332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: N2-3-11.
[2]	"Nucleotide sequence of the streptomycinphosphotransferase and amidinotransferase genes from Streptomyces griseus." Tohyama H., Okami Y., Umezawa H. Nucleic Acids Res. 15:1819-1833(1987) [PubMed: 3029728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23345 / DSM 40236 / IFO 12875 / JCM 4644 / NCIMB 13023.
[3]	"Gene cluster for streptomycin biosynthesis in Streptomyces griseus: analysis of a central region including the major resistance gene." Distler J., Braun C., Ebert A., Piepersberg W. Mol. Gen. Genet. 208:204-210(1987) [PubMed: 3039306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00459 Genomic DNA. Translation: CAA68516.1. X05045 Genomic DNA. Translation: CAA28717.1. X05647 Genomic DNA. Translation: CAA29134.1.
PIR	A26674. KISM6G. S06355.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-14016.
BRENDA	2.7.1.72. 1270.
Family and domain databases
InterPro	IPR006748. NH2Glyco/OHUrea_AB-resist_kin. [Graphical view]
Pfam	PF04655. APH_6_hur. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STRA_STRGR

Accession

Primary (citable) accession number: P08077

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	May 5, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)