P08075 (RMLA_STRGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-1-phosphate thymidylyltransferase
EC=2.7.7.24

Alternative name(s):
Sugar-nucleotidylation enzyme
dTDP-glucose pyrophosphorylase
dTDP-glucose synthase

Gene names

Name:

strD

Organism

Streptomyces griseus

Taxonomic identifier

1911 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis By similarity.
Catalytic activity	dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Antibiotic biosynthesis; streptomycin biosynthesis.
Sequence similarities	Belongs to the glucose-1-phosphate thymidylyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis Streptomycin biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	streptomycin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-1-phosphate thymidylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Glucose-1-phosphate thymidylyltransferase		PRO_0000208005
Sites
	Metal binding	107	1	Magnesium By similarity
	Metal binding	220	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08075 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: ED136AEA854EB7DA	FASTA	355	38,098
	10 20 30 40 50 60 MKALVLAGGT GTRLRPITHT SAKQLVPVAN KPVLFYGLEA IRAAGIIDVG IVVGDTADEI 70 80 90 100 110 120 VAAVGDGSRF GLKVSYIPQS KPLGLAHCVL ISRDFLGEDD FIMYLGDNFV VGVVEDSVRE 130 140 150 160 170 180 FRAARPDAHL MLTRVPEPRS FGVAELSDSG QVLGLEEKPA HPKSDLALVG VYLFSPAIHE 190 200 210 220 230 240 AVAAITPSWR GELEITDAVQ WLIDAGRDVR STVISGYWKD TGNVTDMLEV NRLVLETTEP 250 260 270 280 290 300 RCDGLVDERS DLIGRVLVEE GAEVRNSRVM GPTVIGAGTR VTNSYVGPFT SLAEDCVVED 310 320 330 340 350 SEVEFSIVLR GASISGVRRI EASLIGRHVQ VTSAPEVPHA NRLVLGDHSR AQISS

References

[1]	"Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity." Distler J., Ebert A., Mansouri K., Pissowotzki K., Stockmann M., Piepersberg W. Nucleic Acids Res. 15:8041-8056(1987) [PubMed: 3118332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: N2-3-11.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00459 Genomic DNA. Translation: CAA68514.1.
PIR	A26984.
3D structure databases
ProteinModelPortal	P08075.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005908. G1P_thy_trans_l. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01208. RmlA_long. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RMLA_STRGR

Accession

Primary (citable) accession number: P08075

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program