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P08071

- TRFL_MOUSE

UniProt

P08071 - TRFL_MOUSE

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Protein

Lactotransferrin

Gene
Ltf
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling By similarity.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity By similarity.

Catalytic activityi

Preferential at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Iron 1 By similarity
Active sitei91 – 911 By similarity
Metal bindingi110 – 1101Iron 1 By similarity
Binding sitei135 – 1351Carbonate 1 By similarity
Binding sitei139 – 1391Carbonate 1 By similarity
Binding sitei141 – 1411Carbonate 1; via amide nitrogen By similarity
Binding sitei142 – 1421Carbonate 1; via amide nitrogen By similarity
Metal bindingi210 – 2101Iron 1 By similarity
Metal bindingi271 – 2711Iron 1 By similarity
Active sitei277 – 2771Nucleophile By similarity
Metal bindingi413 – 4131Iron 2 By similarity
Metal bindingi451 – 4511Iron 2 By similarity
Binding sitei477 – 4771Carbonate 2 By similarity
Binding sitei481 – 4811Carbonate 2 By similarity
Binding sitei483 – 4831Carbonate 2; via amide nitrogen By similarity
Binding sitei484 – 4841Carbonate 2; via amide nitrogen By similarity
Metal bindingi544 – 5441Iron 2 By similarity
Metal bindingi613 – 6131Iron 2 By similarity

GO - Molecular functioni

  1. ferric iron binding Source: InterPro
  2. heparin binding Source: Ensembl
  3. peptidase activity Source: MGI
  4. protein serine/threonine kinase activator activity Source: Ensembl
  5. serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. antibacterial humoral response Source: UniProtKB
  2. antifungal humoral response Source: UniProtKB
  3. bone morphogenesis Source: UniProtKB
  4. cellular iron ion homeostasis Source: InterPro
  5. innate immune response in mucosa Source: UniProtKB
  6. iron ion transport Source: InterPro
  7. negative regulation of apoptotic process Source: UniProtKB
  8. negative regulation of lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  9. negative regulation of osteoclast development Source: UniProtKB
  10. negative regulation of single-species biofilm formation in or on host organism Source: UniProtKB
  11. negative regulation of tumor necrosis factor (ligand) superfamily member 11 production Source: UniProtKB
  12. ossification Source: UniProtKB-KW
  13. positive regulation of bone mineralization involved in bone maturation Source: UniProtKB
  14. positive regulation of chondrocyte proliferation Source: UniProtKB
  15. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  16. positive regulation of NF-kappaB transcription factor activity Source: Ensembl
  17. positive regulation of osteoblast differentiation Source: UniProtKB
  18. positive regulation of osteoblast proliferation Source: UniProtKB
  19. positive regulation of toll-like receptor 4 signaling pathway Source: Ensembl
  20. regulation of cytokine production Source: UniProtKB
  21. regulation of tumor necrosis factor production Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Immunity, Ion transport, Iron transport, Osteogenesis, Transport

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198345. Phagosomal maturation (early endosomal stage).
REACT_198563. Amyloids.

Protein family/group databases

MEROPSiS60.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactotransferrin (EC:3.4.21.-)
Short name:
Lactoferrin
Gene namesi
Name:Ltf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:96837. Ltf.

Subcellular locationi

Secreted. Cytoplasmic granule By similarity
Note: Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils By similarity.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular region Source: UniProtKB-SubCell
  3. specific granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 By similarityAdd
BLAST
Chaini20 – 707688LactotransferrinPRO_0000035737Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 63 By similarity
Disulfide bondi37 ↔ 54 By similarity
Glycosylationi118 – 1181N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi133 ↔ 216 By similarity
Disulfide bondi175 ↔ 191 By similarity
Disulfide bondi188 ↔ 199 By similarity
Disulfide bondi249 ↔ 263 By similarity
Disulfide bondi366 ↔ 398 By similarity
Disulfide bondi376 ↔ 389 By similarity
Disulfide bondi423 ↔ 702 By similarity
Disulfide bondi443 ↔ 665 By similarity
Disulfide bondi475 ↔ 550 By similarity
Glycosylationi494 – 4941N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi499 ↔ 693 By similarity
Disulfide bondi509 ↔ 523 By similarity
Disulfide bondi520 ↔ 533 By similarity
Disulfide bondi591 ↔ 605 By similarity
Disulfide bondi643 ↔ 648 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP08071.
PaxDbiP08071.
PRIDEiP08071.

PTM databases

PhosphoSiteiP08071.

Expressioni

Gene expression databases

BgeeiP08071.
CleanExiMM_LTF.
GenevestigatoriP08071.

Interactioni

Subunit structurei

Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1 By similarity.

Protein-protein interaction databases

IntActiP08071. 1 interaction.
MINTiMINT-4138470.

Structurei

3D structure databases

ProteinModelPortaliP08071.
SMRiP08071. Positions 23-707.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 351328Transferrin-like 1Add
BLAST
Domaini363 – 692330Transferrin-like 2Add
BLAST

Sequence similaritiesi

Belongs to the transferrin family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG87503.
GeneTreeiENSGT00390000001619.
HOGENOMiHOG000043759.
HOVERGENiHBG000055.
InParanoidiQ8CBA0.
KOiK17283.
OMAiRPVEGYL.
OrthoDBiEOG7D59N7.
TreeFamiTF324013.

Family and domain databases

InterProiIPR016357. Transferrin.
IPR001156. Transferrin-like_dom.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PfamiPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFiPIRSF002549. Transferrin. 1 hit.
PRINTSiPR00422. TRANSFERRIN.
SMARTiSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEiPS00205. TRANSFERRIN_LIKE_1. 1 hit.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08071-1 [UniParc]FASTAAdd to Basket

« Hide

MRLLIPSLIF LEALGLCLAK ATTVQWCAVS NSEEEKCLRW QNEMRKVGGP    50
PLSCVKKSST RQCIQAIVTN RADAMTLDGG TMFDAGKPPY KLRPVAAEVY 100
GTKEQPRTHY YAVAVVKNSS NFHLNQLQGL RSCHTGIGRS AGWKIPIGTL 150
RPYLNWNGPP ASLEEAVSKF FSKSCVPGAQ KDRFPNLCSS CAGTGANKCA 200
SSPEEPYSGY AGALRCLRDN AGDVAFTRGS TVFEELPNKA ERDQYKLLCP 250
DNTWKPVTEY KECHLAQVPS HAVVSRSTND KEEAIWELLR QSQEKFGKKQ 300
ASGFQLFASP SGQKDLLFKE SAIGFVRVPQ KVDVGLYLTF SYTTSIQNLN 350
KKQQDVIASK ARVTWCAVGS EEKRKCDQWN RASRGRVTCI SFPTTEDCIV 400
AIMKGDADAM SLDGGYIYTA GKCGLVPVLA ENQKSSKSNG LDCVNRPVEG 450
YLAVAAVRRE DAGFTWSSLR GKKSCHTAVD RTAGWNIPMG LLANQTRSCK 500
FNEFFSQSCA PGADPKSNLC ALCIGDEKGE NKCAPNSKER YQGYTGALRC 550
LAEKAGNVAF LKDSTVLQNT DGKNTEEWAR NLKLKDFELL CLDDTRKPVT 600
EAKNCHLAIA PNHAVVSRTD KVEVLQQVLL DQQVQFGRNG QRCPGEFCLF 650
QSKTKNLLFN DNTECLAKIP GKTTSEKYLG KEYVIATERL KQCSSSPLLE 700
ACAFLTQ 707
Length:707
Mass (Da):77,838
Last modified:July 27, 2011 - v4
Checksum:iE1B32F5FD8748A0F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 22MR → IQG in AAA40525. 1 Publication
Sequence conflicti25 – 251Q → R in AAA40525. 1 Publication
Sequence conflicti25 – 251Q → R in AAH06904. 1 Publication
Sequence conflicti82 – 821M → L in BAA13633. 1 Publication
Sequence conflicti359 – 3591S → T in BAA13633. 1 Publication
Sequence conflicti382 – 3821A → D in AAA40525. 1 Publication
Sequence conflicti449 – 4491E → G in BAA13633. 1 Publication
Sequence conflicti629 – 6291L → V in AAA40525. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03298 mRNA. Translation: AAA40525.1.
D88510 mRNA. Translation: BAA13633.1.
AK036491 mRNA. Translation: BAC29450.1.
AK144556 mRNA. Translation: BAE25936.1.
AK151822 mRNA. Translation: BAE30719.1.
BC006904 mRNA. Translation: AAH06904.1.
M74778 Genomic DNA. Translation: AAA39427.1.
CCDSiCCDS23581.1.
PIRiA28438.
RefSeqiNP_032548.2. NM_008522.3.
UniGeneiMm.282359.

Genome annotation databases

EnsembliENSMUST00000035077; ENSMUSP00000035077; ENSMUSG00000032496.
GeneIDi17002.
KEGGimmu:17002.
UCSCiuc009rvj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03298 mRNA. Translation: AAA40525.1 .
D88510 mRNA. Translation: BAA13633.1 .
AK036491 mRNA. Translation: BAC29450.1 .
AK144556 mRNA. Translation: BAE25936.1 .
AK151822 mRNA. Translation: BAE30719.1 .
BC006904 mRNA. Translation: AAH06904.1 .
M74778 Genomic DNA. Translation: AAA39427.1 .
CCDSi CCDS23581.1.
PIRi A28438.
RefSeqi NP_032548.2. NM_008522.3.
UniGenei Mm.282359.

3D structure databases

ProteinModelPortali P08071.
SMRi P08071. Positions 23-707.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08071. 1 interaction.
MINTi MINT-4138470.

Protein family/group databases

MEROPSi S60.001.

PTM databases

PhosphoSitei P08071.

Proteomic databases

MaxQBi P08071.
PaxDbi P08071.
PRIDEi P08071.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035077 ; ENSMUSP00000035077 ; ENSMUSG00000032496 .
GeneIDi 17002.
KEGGi mmu:17002.
UCSCi uc009rvj.1. mouse.

Organism-specific databases

CTDi 4057.
MGIi MGI:96837. Ltf.

Phylogenomic databases

eggNOGi NOG87503.
GeneTreei ENSGT00390000001619.
HOGENOMi HOG000043759.
HOVERGENi HBG000055.
InParanoidi Q8CBA0.
KOi K17283.
OMAi RPVEGYL.
OrthoDBi EOG7D59N7.
TreeFami TF324013.

Enzyme and pathway databases

Reactomei REACT_198345. Phagosomal maturation (early endosomal stage).
REACT_198563. Amyloids.

Miscellaneous databases

ChiTaRSi LTF. mouse.
NextBioi 291116.
PROi P08071.
SOURCEi Search...

Gene expression databases

Bgeei P08071.
CleanExi MM_LTF.
Genevestigatori P08071.

Family and domain databases

InterProi IPR016357. Transferrin.
IPR001156. Transferrin-like_dom.
IPR018195. Transferrin_Fe_BS.
[Graphical view ]
Pfami PF00405. Transferrin. 2 hits.
[Graphical view ]
PIRSFi PIRSF002549. Transferrin. 1 hit.
PRINTSi PR00422. TRANSFERRIN.
SMARTi SM00094. TR_FER. 2 hits.
[Graphical view ]
PROSITEi PS00205. TRANSFERRIN_LIKE_1. 1 hit.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Lactotransferrin is the major estrogen inducible protein of mouse uterine secretions."
    Pentecost B.T., Teng C.T.
    J. Biol. Chem. 262:10134-10139(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Uterus.
  2. Moriishi K.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Uterus.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone, Bone marrow and Gall bladder.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Characterization of estrogen-responsive mouse lactoferrin promoter."
    Liu Y., Teng C.T.
    J. Biol. Chem. 266:21880-21885(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.

Entry informationi

Entry nameiTRFL_MOUSE
AccessioniPrimary (citable) accession number: P08071
Secondary accession number(s): P70690
, Q61799, Q8CBA0, Q922P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 129 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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