Skip Header

Contribute Send feedback
Read comments (?) or add your own

P08067 (UCRI_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial

EC=1.10.2.2
Alternative name(s):
Complex III subunit 5
Rieske iron-sulfur protein
Short name=RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Gene names
Name:RIP1
Ordered Locus Names:YEL024W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c.

Catalytic activity

QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.

Cofactor

Binds 1 2Fe-2S cluster per subunit.

Subunit structure

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.

Subcellular location

Mitochondrion.

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Sequence similarities

Contains 1 Rieske domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3030Mitochondrion
Chain31 – 215185Cytochrome b-c1 complex subunit Rieske, mitochondrial
PRO_0000030683

Regions

Domain123 – 21492Rieske

Sites

Metal binding1591Iron-sulfur (2Fe-2S)
Metal binding1611Iron-sulfur (2Fe-2S); via pros nitrogen
Metal binding1781Iron-sulfur (2Fe-2S)
Metal binding1811Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond164 ↔ 180 By similarity

Experimental info

Mutagenesis1571G → D: Loss of activity.
Mutagenesis1591C → S: Loss of activity.
Mutagenesis1611H → R: Loss of activity.
Mutagenesis1631G → D: Partial loss of activity.
Mutagenesis1641C → S: Loss of activity.
Mutagenesis1661P → L: Partial loss of activity.
Mutagenesis1781C → S or Y: Loss of activity.
Mutagenesis1791P → L: Partial loss of activity.
Mutagenesis1801C → S: Loss of activity.
Mutagenesis1811H → R: Loss of activity.
Mutagenesis1831S → L: Loss of activity.
Mutagenesis1841H → R: No loss of activity.
Mutagenesis1861D → N: Partial loss of activity.
Mutagenesis1891G → D: Loss of activity.
Mutagenesis1951P → S: No loss of activity.
Mutagenesis1961A → T: No loss of activity.
Mutagenesis2031P → S: Loss of activity.

Secondary structure

.................................... 215
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08067 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 21981BD8492E86F3

FASTA21523,365
        10         20         30         40         50         60 
MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD KGRSYAYFMV 

        70         80         90        100        110        120 
GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA IPLGKNVVVK WQGKPVFIRH 

       130        140        150        160        170        180 
RTPHEIQEAN SVDMSALKDP QTDADRVKDP QWLIMLGICT HLGCVPIGEA GDFGGWFCPC 

       190        200        210 
HGSHYDISGR IRKGPAPLNL EIPAYEFDGD KVIVG 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the nuclear gene encoding the Rieske iron-sulfur protein (RIP1) from Saccharomyces cerevisiae."
Beckmann J.D., Ljungdahl P.O., Lopez J.L., Trumpower B.L.
J. Biol. Chem. 262:8901-8909(1987) [PubMed: 3036836] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 64665 / S288c / DC5.
[2]"Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. I. Construction of a RIP1 deletion strain and isolation of temperature-sensitive mutants."
Beckmann J.D., Ljungdahl P.O., Trumpower B.L.
J. Biol. Chem. 264:3713-3722(1989) [PubMed: 2645276] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. III. Import, protease processing, and assembly into the cytochrome bc1 complex of iron-sulfur protein lacking the iron-sulfur cluster."
Graham L.A., Trumpower B.L.
J. Biol. Chem. 266:22485-22492(1991) [PubMed: 1657998] [Abstract]
Cited for: MUTAGENESIS.
[7]"Structure and function of the mitochondrial bc1 complex. A mutational analysis of the yeast Rieske iron-sulfur protein."
Gatti D.L., Meinhardt S.W., Ohnishi T., Tzagoloff A.
J. Mol. Biol. 205:421-435(1989) [PubMed: 2538628] [Abstract]
Cited for: MUTAGENESIS.
Strain: D273-10B/A1.
[8]"Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment."
Hunte C., Koepke J., Lange C., Rossmanith T., Michel H.
Structure 8:669-684(2000) [PubMed: 10873857] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[9]"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c."
Lange C., Hunte C.
Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed: 11880631] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M23316 Genomic DNA. Translation: AAA34980.1.
M24500 Genomic DNA. Translation: AAA34981.1.
U18530 Genomic DNA. Translation: AAB64501.1.
AY558341 Genomic DNA. Translation: AAS56667.1.
BK006939 Genomic DNA. Translation: DAA07628.1.
PIRA29318.
RefSeqNP_010890.1. NM_001178839.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30E31-215[»]
1KB9X-ray2.30E31-215[»]
1KYOX-ray2.97E/P31-215[»]
1P84X-ray2.50E31-215[»]
2IBZX-ray2.30E31-215[»]
3CX5X-ray1.90E/P31-215[»]
3CXHX-ray2.50E/P31-215[»]
ProteinModelPortalP08067.
SMRP08067. Positions 31-215.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6616N.
IntActP08067. 3 interactions.
MINTMINT-696377.
STRINGP08067.

Proteomic databases

PeptideAtlasP08067.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL024W; YEL024W; YEL024W.
GeneID856689.
KEGGsce:YEL024W.
NMPDRfig|4932.3.peg.1944.

Organism-specific databases

CYGDYEL024w.
SGDS000000750. RIP1.

Phylogenomic databases

eggNOGfuNOG04491.
GeneTreeEFGT00050000006359.
HOGENOMHBG668715.
OMARSYAYFM.
OrthoDBEOG46T69W.

Gene expression databases

ArrayExpressP08067.
GenevestigatorP08067.
GermOnlineYEL024W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
Gene3DG3DSA:2.102.10.10. Rieske_reg. 1 hit.
G3DSA:1.20.5.270. Ubiquinol_cyt_Rdtase_TM. 1 hit.
KOK00411.
PANTHERPTHR10134. Rieske. 1 hit.
PfamPF00355. Rieske. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSPR00162. RIESKE.
SUPFAMSSF50022. Rieske_dom. 1 hit.
SSF81502. UCR_TM_region. 1 hit.
TIGRFAMsTIGR01416. Rieske_proteo. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982730.

Entry information

Entry nameUCRI_YEAST
AccessionPrimary (citable) accession number: P08067
Secondary accession number(s): D3DLM4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: December 14, 2011
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families