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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

RIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c.

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi159 – 1591Iron-sulfur (2Fe-2S)
Metal bindingi161 – 1611Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi178 – 1781Iron-sulfur (2Fe-2S)
Metal bindingi181 – 1811Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: SGD
  • mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YEL024W-MONOMER.

Protein family/group databases

TCDBi3.D.3.3.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Gene namesi
Name:RIP1
Ordered Locus Names:YEL024W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL024W.
SGDiS000000750. RIP1.

Subcellular locationi

  • Mitochondrion inner membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei49 – 8234HelicalAdd
BLAST

GO - Cellular componenti

  • mitochondrial respiratory chain complex III Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi157 – 1571G → D: Loss of activity.
Mutagenesisi159 – 1591C → S: Loss of activity.
Mutagenesisi161 – 1611H → R: Loss of activity.
Mutagenesisi163 – 1631G → D: Partial loss of activity.
Mutagenesisi164 – 1641C → S: Loss of activity.
Mutagenesisi166 – 1661P → L: Partial loss of activity.
Mutagenesisi178 – 1781C → S or Y: Loss of activity.
Mutagenesisi179 – 1791P → L: Partial loss of activity.
Mutagenesisi180 – 1801C → S: Loss of activity.
Mutagenesisi181 – 1811H → R: Loss of activity.
Mutagenesisi183 – 1831S → L: Loss of activity.
Mutagenesisi184 – 1841H → R: No loss of activity.
Mutagenesisi186 – 1861D → N: Partial loss of activity.
Mutagenesisi189 – 1891G → D: Loss of activity.
Mutagenesisi195 – 1951P → S: No loss of activity.
Mutagenesisi196 – 1961A → T: No loss of activity.
Mutagenesisi203 – 2031P → S: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionAdd
BLAST
Chaini31 – 215185Cytochrome b-c1 complex subunit Rieske, mitochondrialPRO_0000030683Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi164 ↔ 180PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP08067.

Interactioni

Subunit structurei

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.

Protein-protein interaction databases

BioGridi36705. 96 interactions.
DIPiDIP-6616N.
IntActiP08067. 2 interactions.
MINTiMINT-696377.

Structurei

Secondary structure

1
215
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni40 – 423Combined sources
Beta strandi46 – 483Combined sources
Helixi51 – 8030Combined sources
Helixi86 – 883Combined sources
Beta strandi94 – 974Combined sources
Helixi98 – 1003Combined sources
Beta strandi106 – 1116Combined sources
Beta strandi114 – 1207Combined sources
Helixi123 – 1308Combined sources
Helixi134 – 1363Combined sources
Turni144 – 1463Combined sources
Beta strandi152 – 1565Combined sources
Turni160 – 1623Combined sources
Beta strandi167 – 1704Combined sources
Turni171 – 1744Combined sources
Beta strandi175 – 1784Combined sources
Turni179 – 1824Combined sources
Beta strandi183 – 1853Combined sources
Beta strandi191 – 1955Combined sources
Beta strandi205 – 2084Combined sources
Beta strandi211 – 2144Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30E31-215[»]
1KB9X-ray2.30E31-215[»]
1KYOX-ray2.97E/P31-215[»]
1P84X-ray2.50E31-215[»]
2IBZX-ray2.30E31-215[»]
3CX5X-ray1.90E/P31-215[»]
3CXHX-ray2.50E/P31-215[»]
4PD4X-ray3.04E31-215[»]
DisProtiDP00687.
ProteinModelPortaliP08067.
SMRiP08067. Positions 31-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08067.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini123 – 21492RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000001014.
HOGENOMiHOG000255193.
InParanoidiP08067.
KOiK00411.
OMAiLYSYFMV.
OrthoDBiEOG7XWQ0B.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD
60 70 80 90 100
KGRSYAYFMV GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA
110 120 130 140 150
IPLGKNVVVK WQGKPVFIRH RTPHEIQEAN SVDMSALKDP QTDADRVKDP
160 170 180 190 200
QWLIMLGICT HLGCVPIGEA GDFGGWFCPC HGSHYDISGR IRKGPAPLNL
210
EIPAYEFDGD KVIVG
Length:215
Mass (Da):23,365
Last modified:August 1, 1988 - v1
Checksum:i21981BD8492E86F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23316 Genomic DNA. Translation: AAA34980.1.
M24500 Genomic DNA. Translation: AAA34981.1.
U18530 Genomic DNA. Translation: AAB64501.1.
AY558341 Genomic DNA. Translation: AAS56667.1.
BK006939 Genomic DNA. Translation: DAA07628.1.
PIRiA29318.
RefSeqiNP_010890.3. NM_001178839.3.

Genome annotation databases

EnsemblFungiiYEL024W; YEL024W; YEL024W.
GeneIDi856689.
KEGGisce:YEL024W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23316 Genomic DNA. Translation: AAA34980.1.
M24500 Genomic DNA. Translation: AAA34981.1.
U18530 Genomic DNA. Translation: AAB64501.1.
AY558341 Genomic DNA. Translation: AAS56667.1.
BK006939 Genomic DNA. Translation: DAA07628.1.
PIRiA29318.
RefSeqiNP_010890.3. NM_001178839.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30E31-215[»]
1KB9X-ray2.30E31-215[»]
1KYOX-ray2.97E/P31-215[»]
1P84X-ray2.50E31-215[»]
2IBZX-ray2.30E31-215[»]
3CX5X-ray1.90E/P31-215[»]
3CXHX-ray2.50E/P31-215[»]
4PD4X-ray3.04E31-215[»]
DisProtiDP00687.
ProteinModelPortaliP08067.
SMRiP08067. Positions 31-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36705. 96 interactions.
DIPiDIP-6616N.
IntActiP08067. 2 interactions.
MINTiMINT-696377.

Protein family/group databases

TCDBi3.D.3.3.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Proteomic databases

MaxQBiP08067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL024W; YEL024W; YEL024W.
GeneIDi856689.
KEGGisce:YEL024W.

Organism-specific databases

EuPathDBiFungiDB:YEL024W.
SGDiS000000750. RIP1.

Phylogenomic databases

GeneTreeiENSGT00390000001014.
HOGENOMiHOG000255193.
InParanoidiP08067.
KOiK00411.
OMAiLYSYFMV.
OrthoDBiEOG7XWQ0B.

Enzyme and pathway databases

BioCyciYEAST:YEL024W-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08067.
PROiP08067.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the nuclear gene encoding the Rieske iron-sulfur protein (RIP1) from Saccharomyces cerevisiae."
    Beckmann J.D., Ljungdahl P.O., Lopez J.L., Trumpower B.L.
    J. Biol. Chem. 262:8901-8909(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 64665 / S288c / DC5.
  2. "Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. I. Construction of a RIP1 deletion strain and isolation of temperature-sensitive mutants."
    Beckmann J.D., Ljungdahl P.O., Trumpower B.L.
    J. Biol. Chem. 264:3713-3722(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. III. Import, protease processing, and assembly into the cytochrome bc1 complex of iron-sulfur protein lacking the iron-sulfur cluster."
    Graham L.A., Trumpower B.L.
    J. Biol. Chem. 266:22485-22492(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  7. "Structure and function of the mitochondrial bc1 complex. A mutational analysis of the yeast Rieske iron-sulfur protein."
    Gatti D.L., Meinhardt S.W., Ohnishi T., Tzagoloff A.
    J. Mol. Biol. 205:421-435(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
    Strain: D273-10B/A1.
  8. "Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment."
    Hunte C., Koepke J., Lange C., Rossmanith T., Michel H.
    Structure 8:669-684(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  9. "Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c."
    Lange C., Hunte C.
    Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
  10. "Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer."
    Solmaz S.R., Hunte C.
    J. Biol. Chem. 283:17542-17549(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 31-215 IN THE BC1 COMPLEX, DISULFIDE BOND, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiUCRI_YEAST
AccessioniPrimary (citable) accession number: P08067
Secondary accession number(s): D3DLM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 6, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.