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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

RIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c.2 Publications

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.1 Publication

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.2 Publications

Cofactori

[2Fe-2S] cluster3 PublicationsNote: Binds 1 [2Fe-2S] cluster per subunit.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi159Iron-sulfur (2Fe-2S)Combined sources3 Publications1
Metal bindingi161Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources3 Publications1
Metal bindingi178Iron-sulfur (2Fe-2S)Combined sources3 Publications1
Metal bindingi181Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources3 Publications1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ubiquinol-cytochrome-c reductase activity Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YEL024W-MONOMER

Protein family/group databases

TCDBi3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.22 Publications)
Alternative name(s):
Complex III subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Gene namesi
Name:RIP1
Ordered Locus Names:YEL024W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL024W
SGDiS000000750 RIP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei49 – 82HelicalSequence analysisAdd BLAST34

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157G → D: Loss of activity. 1 Publication1
Mutagenesisi159C → S: Loss of activity. 1 Publication1
Mutagenesisi161H → R: Loss of activity. 1 Publication1
Mutagenesisi163G → D: Partial loss of activity. 1 Publication1
Mutagenesisi164C → S: Loss of activity. 1 Publication1
Mutagenesisi166P → L: Partial loss of activity. 1 Publication1
Mutagenesisi178C → S or Y: Loss of activity. 2 Publications1
Mutagenesisi179P → L: Partial loss of activity. 1 Publication1
Mutagenesisi180C → S: Loss of activity. 1 Publication1
Mutagenesisi181H → R: Loss of activity. 1 Publication1
Mutagenesisi183S → L: Loss of activity. 1 Publication1
Mutagenesisi184H → R: No loss of activity. 1 Publication1
Mutagenesisi186D → N: Partial loss of activity. 1 Publication1
Mutagenesisi189G → D: Loss of activity. 1 Publication1
Mutagenesisi195P → S: No loss of activity. 1 Publication1
Mutagenesisi196A → T: No loss of activity. 1 Publication1
Mutagenesisi203P → S: Loss of activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionSequence analysisAdd BLAST30
ChainiPRO_000003068331 – 215Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi164 ↔ 180PROSITE-ProRule annotationCombined sources3 Publications

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP08067
PaxDbiP08067
PRIDEiP08067

Interactioni

Subunit structurei

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.3 Publications

Protein-protein interaction databases

BioGridi36705, 245 interactors
ComplexPortaliCPX-567 Mitochondrial electron transport complex III
DIPiDIP-6616N
IntActiP08067, 3 interactors
STRINGi4932.YEL024W

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni40 – 42Combined sources3
Beta strandi46 – 48Combined sources3
Helixi51 – 80Combined sources30
Helixi86 – 88Combined sources3
Beta strandi94 – 97Combined sources4
Helixi98 – 100Combined sources3
Beta strandi106 – 111Combined sources6
Beta strandi114 – 120Combined sources7
Helixi123 – 130Combined sources8
Helixi134 – 136Combined sources3
Turni144 – 146Combined sources3
Beta strandi152 – 156Combined sources5
Turni160 – 162Combined sources3
Beta strandi167 – 170Combined sources4
Turni171 – 174Combined sources4
Beta strandi175 – 178Combined sources4
Turni179 – 182Combined sources4
Beta strandi183 – 185Combined sources3
Beta strandi191 – 195Combined sources5
Beta strandi205 – 208Combined sources4
Beta strandi211 – 214Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30E31-215[»]
1KB9X-ray2.30E31-215[»]
1KYOX-ray2.97E/P31-215[»]
1P84X-ray2.50E31-215[»]
2IBZX-ray2.30E31-215[»]
3CX5X-ray1.90E/P31-215[»]
3CXHX-ray2.50E/P31-215[»]
4PD4X-ray3.04E31-215[»]
DisProtiDP00687
ProteinModelPortaliP08067
SMRiP08067
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08067

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 214RieskePROSITE-ProRule annotationAdd BLAST92

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000001014
HOGENOMiHOG000255193
InParanoidiP08067
KOiK00411
OMAiVFHCPCH
OrthoDBiEOG092C4QML

Family and domain databases

Gene3Di1.20.5.270, 1 hit
2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR037008 bc1_Rieske_TM_sf
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR014349 Rieske_Fe-S_prot
IPR005805 Rieske_Fe-S_prot_C
IPR004192 Rieske_TM
IPR006317 Ubiquinol_cyt_c_Rdtase_Fe-S-su
PANTHERiPTHR10134 PTHR10134, 1 hit
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF02921 UCR_TM, 1 hit
PRINTSiPR00162 RIESKE
SUPFAMiSSF50022 SSF50022, 1 hit
TIGRFAMsiTIGR01416 Rieske_proteo, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD
60 70 80 90 100
KGRSYAYFMV GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA
110 120 130 140 150
IPLGKNVVVK WQGKPVFIRH RTPHEIQEAN SVDMSALKDP QTDADRVKDP
160 170 180 190 200
QWLIMLGICT HLGCVPIGEA GDFGGWFCPC HGSHYDISGR IRKGPAPLNL
210
EIPAYEFDGD KVIVG
Length:215
Mass (Da):23,365
Last modified:August 1, 1988 - v1
Checksum:i21981BD8492E86F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23316 Genomic DNA Translation: AAA34980.1
M24500 Genomic DNA Translation: AAA34981.1
U18530 Genomic DNA Translation: AAB64501.1
AY558341 Genomic DNA Translation: AAS56667.1
BK006939 Genomic DNA Translation: DAA07628.1
PIRiA29318
RefSeqiNP_010890.3, NM_001178839.3

Genome annotation databases

EnsemblFungiiYEL024W; YEL024W; YEL024W
GeneIDi856689
KEGGisce:YEL024W

Similar proteinsi

Entry informationi

Entry nameiUCRI_YEAST
AccessioniPrimary (citable) accession number: P08067
Secondary accession number(s): D3DLM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 20, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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