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Protein

Succinate dehydrogenase iron-sulfur subunit

Gene

sdhB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (bacterial route).
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase flavoprotein subunit (sdhA), Succinate dehydrogenase iron-sulfur subunit (sdhB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (bacterial route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi69Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi84Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi161Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi165Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi212Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi218Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi222Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28430-MONOMER.
UniPathwayiUPA00223; UER01005.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase iron-sulfur subunit (EC:1.3.5.1)
Gene namesi
Name:sdhB
Ordered Locus Names:BSU28430
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001586862 – 253Succinate dehydrogenase iron-sulfur subunitAdd BLAST252

Proteomic databases

PaxDbiP08066.
PRIDEiP08066.

Interactioni

Subunit structurei

In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015526.

Structurei

3D structure databases

ProteinModelPortaliP08066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 1744Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST29

Sequence similaritiesi

Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105E33. Bacteria.
COG0479. LUCA.
HOGENOMiHOG000160588.
InParanoidiP08066.
KOiK00240.
OMAiFKNFFGS.
PhylomeDBiP08066.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQKTIRFI ITRQDTADST PYDEEFEIPY RPNLNVISAL MEIRRNPVNV
60 70 80 90 100
KGEKTTPVTW DMNCLEEVCG ACSMVINGKP RQSCTALIDQ LEQPIRLKPM
110 120 130 140 150
KTFPVVRDLQ VDRSRMFDSL KKVKAWIPID GTYDLGPGPR MPEKRRQWAY
160 170 180 190 200
ELSKCMTCGV CLEACPNVNS KSKFMGPAPM SQVRLFNAHP TGAMNKSERL
210 220 230 240 250
EALMDEGGLA DCGNSQNCVQ SCPKGIPLTT SIAALNRDTN LQAFRNFFGS

DRV
Length:253
Mass (Da):28,418
Last modified:January 23, 2007 - v3
Checksum:iC20E095CDD960B3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13470 Genomic DNA. Translation: AAA22747.1.
Z75208 Genomic DNA. Translation: CAA99548.1.
AL009126 Genomic DNA. Translation: CAB14803.1.
M17642 Genomic DNA. Translation: AAA22471.1.
PIRiB27763.
RefSeqiNP_390721.1. NC_000964.3.
WP_003229569.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14803; CAB14803; BSU28430.
GeneIDi937460.
KEGGibsu:BSU28430.
PATRICi18977570. VBIBacSub10457_2973.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13470 Genomic DNA. Translation: AAA22747.1.
Z75208 Genomic DNA. Translation: CAA99548.1.
AL009126 Genomic DNA. Translation: CAB14803.1.
M17642 Genomic DNA. Translation: AAA22471.1.
PIRiB27763.
RefSeqiNP_390721.1. NC_000964.3.
WP_003229569.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP08066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015526.

Proteomic databases

PaxDbiP08066.
PRIDEiP08066.

Protocols and materials databases

DNASUi937460.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14803; CAB14803; BSU28430.
GeneIDi937460.
KEGGibsu:BSU28430.
PATRICi18977570. VBIBacSub10457_2973.

Phylogenomic databases

eggNOGiENOG4105E33. Bacteria.
COG0479. LUCA.
HOGENOMiHOG000160588.
InParanoidiP08066.
KOiK00240.
OMAiFKNFFGS.
PhylomeDBiP08066.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01005.
BioCyciBSUB:BSU28430-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDHB_BACSU
AccessioniPrimary (citable) accession number: P08066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.