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P08066 (SDHB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinate dehydrogenase iron-sulfur subunit

EC=1.3.5.1
Gene names
Name:sdhB
Ordered Locus Names:BSU28430
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Succinate + a quinone = fumarate + a quinol.

Cofactor

Binds 1 2Fe-2S cluster.

Binds 1 3Fe-4S cluster.

Binds 1 4Fe-4S cluster.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.

Subunit structure

In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

Sequence similarities

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Contains 1 4Fe-4S ferredoxin-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 253252Succinate dehydrogenase iron-sulfur subunit
PRO_0000158686

Regions

Domain146 – 174294Fe-4S ferredoxin-type

Sites

Metal binding641Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding691Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding841Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1551Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1581Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1611Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1651Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2121Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2181Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2221Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P08066 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: C20E095CDD960B3E

FASTA25328,418
        10         20         30         40         50         60 
MSEQKTIRFI ITRQDTADST PYDEEFEIPY RPNLNVISAL MEIRRNPVNV KGEKTTPVTW 

        70         80         90        100        110        120 
DMNCLEEVCG ACSMVINGKP RQSCTALIDQ LEQPIRLKPM KTFPVVRDLQ VDRSRMFDSL 

       130        140        150        160        170        180 
KKVKAWIPID GTYDLGPGPR MPEKRRQWAY ELSKCMTCGV CLEACPNVNS KSKFMGPAPM 

       190        200        210        220        230        240 
SQVRLFNAHP TGAMNKSERL EALMDEGGLA DCGNSQNCVQ SCPKGIPLTT SIAALNRDTN 

       250 
LQAFRNFFGS DRV 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex."
Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.
J. Bacteriol. 169:864-873(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / PY79.
[2]"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"The nucleotide sequence and the transcription during sporulation of the gerE gene of Bacillus subtilis."
Cutting S.M., Mandelstam J.
J. Gen. Microbiol. 132:3013-3024(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-253.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13470 Genomic DNA. Translation: AAA22747.1.
Z75208 Genomic DNA. Translation: CAA99548.1.
AL009126 Genomic DNA. Translation: CAB14803.1.
M17642 Genomic DNA. Translation: AAA22471.1.
PIRB27763.
RefSeqNP_390721.1. NC_000964.3.

3D structure databases

ProteinModelPortalP08066.
SMRP08066. Positions 1-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU28430.

Proteomic databases

PaxDbP08066.

Protocols and materials databases

DNASU937460.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14803; CAB14803; BSU28430.
GeneID937460.
KEGGbsu:BSU28430.
PATRIC18977570. VBIBacSub10457_2973.

Organism-specific databases

GenoListBSU28430. [Micado]

Phylogenomic databases

eggNOGCOG0479.
HOGENOMHOG000160588.
KOK00240.
OMAQYEKIRP.
OrthoDBEOG6CS02W.
PhylomeDBP08066.

Enzyme and pathway databases

BioCycBSUB:BSU28430-MONOMER.
UniPathwayUPA00223; UER01005.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamPF13085. Fer2_3. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR00384. dhsB. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSDHB_BACSU
AccessionPrimary (citable) accession number: P08066
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList