Reviewed, UniProtKB/Swiss-Prot P08066 (DHSB_BACSU)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:	sdhB
Ordered Locus Names:	BSU28430

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 3Fe-4S cluster. Binds 1 4Fe-4S cluster.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 253	252	Succinate dehydrogenase iron-sulfur subunit		PRO_0000158686
Regions
	Domain	146 – 174	29	4Fe-4S ferredoxin-type
Sites
	Metal binding	64	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	69	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	72	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	84	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	155	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	158	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	161	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	165	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	212	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	218	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	222	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08066-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C20E095CDD960B3E	FASTA	253	28,418
	10 20 30 40 50 60 MSEQKTIRFI ITRQDTADST PYDEEFEIPY RPNLNVISAL MEIRRNPVNV KGEKTTPVTW 70 80 90 100 110 120 DMNCLEEVCG ACSMVINGKP RQSCTALIDQ LEQPIRLKPM KTFPVVRDLQ VDRSRMFDSL 130 140 150 160 170 180 KKVKAWIPID GTYDLGPGPR MPEKRRQWAY ELSKCMTCGV CLEACPNVNS KSKFMGPAPM 190 200 210 220 230 240 SQVRLFNAHP TGAMNKSERL EALMDEGGLA DCGNSQNCVQ SCPKGIPLTT SIAALNRDTN 250 LQAFRNFFGS DRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex." Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R. J. Bacteriol. 169:864-873(1987) [PubMed: 3027051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / PY79.
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"The nucleotide sequence and the transcription during sporulation of the gerE gene of Bacillus subtilis." Cutting S.M., Mandelstam J. J. Gen. Microbiol. 132:3013-3024(1986) [PubMed: 3114423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M13470 Genomic DNA. Translation: AAA22747.1. Z75208 Genomic DNA. Translation: CAA99548.1. AL009126 Genomic DNA. Translation: CAB14803.1. M17642 Genomic DNA. Translation: AAA22471.1.
PIR	B27763.
RefSeq	NP_390721.1.
3D structure databases
HSSP	HSSP built from PDB template 1QLA based on UniProtKB P17596.
ModBase	Search...
Genome annotation databases
GeneID	937460.
GenomeReviews	Gene locus BSU28430 in contig AL009126_GR.
KEGG	bsu:BSU28430.
NMPDR	fig|224308.1.peg.2846.
Organism-specific databases
SubtiList	BG10353. sdhB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P08066.
OMA	EPMSTFP.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2839-MON.
BRENDA	1.3.99.1. 150.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. b-grasp_ferredoxin-like. IPR012285. Fum_reductase_C. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB_BACSU

Accession

Primary (citable) accession number: P08066

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)