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P08065 (SDHA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinate dehydrogenase flavoprotein subunit

EC=1.3.5.1
Gene names
Name:sdhA
Synonyms:citF
Ordered Locus Names:BSU28440
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length586 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Succinate + a quinone = fumarate + a quinol.

Cofactor

FAD.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.

Subunit structure

In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
   Biological processElectron transport
Transport
Tricarboxylic acid cycle
   Cellular componentCell membrane
Membrane
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsuccinate dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 586585Succinate dehydrogenase flavoprotein subunit
PRO_0000158650

Regions

Nucleotide binding10 – 156FAD By similarity
Nucleotide binding33 – 4816FAD By similarity
Nucleotide binding391 – 3922FAD By similarity

Sites

Active site2851Proton acceptor By similarity
Binding site2361Substrate By similarity
Binding site2501Substrate By similarity
Binding site3521Substrate By similarity
Binding site3761FAD By similarity
Binding site3861Substrate By similarity

Amino acid modifications

Modified residue411Tele-8alpha-FAD histidine By similarity

Natural variations

Natural variant481G → D in a defective mutant. Ref.6

Experimental info

Sequence conflict821V → L in AAA22746. Ref.1
Sequence conflict821V → L in CAA99547. Ref.2
Sequence conflict1001G → A in AAA22746. Ref.1
Sequence conflict1001G → A in CAA99547. Ref.2
Sequence conflict388 – 3903GAN → ERT in AAA22746. Ref.1
Sequence conflict388 – 3903GAN → ERT in CAA99547. Ref.2
Sequence conflict4411S → I in AAA22746. Ref.1
Sequence conflict4411S → I in CAA99547. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08065 [UniParc].

Last modified July 7, 2009. Version 4.
Checksum: F2327C2B383DE671

FASTA58665,152
        10         20         30         40         50         60 
MSQSSIIVVG GGLAGLMATI KAAESGMAVK LFSIVPVKRS HSVCAQGGIN GAVNTKGEGD 

        70         80         90        100        110        120 
SPWEHFDDTV YGGDFLANQP PVKAMCEAAP SIIHLLDRMG VMFNRTPEGL LDFRRFGGTQ 

       130        140        150        160        170        180 
HHRTAYAGAT TGQQLLYALD EQVRRYEVAG LVTKYEGWEF LGAVLDDDRT CRGIVAQNLT 

       190        200        210        220        230        240 
NMQIESFRSD AVIMATGGPG IIFGKSTNSM INTGSAASIV YQQGAYYANG EFIQIHPTAI 

       250        260        270        280        290        300 
PGDDKLRLMS ESARGEGGRV WTYKDGKPWY FLEEKYPAYG NLVPRDIATR EIFDVCVNQK 

       310        320        330        340        350        360 
LGINGENMVY LDLSHKDPKE LDIKLGGIIE IYEKFMGDDP RKLPMKIFPA VHYSMGGLWV 

       370        380        390        400        410        420 
DYDQMTNIPG LFAAGECDYS MHGGNRLGAN SLLSAIYGGM VAGPNAVKYV NGLESSAEDM 

       430        440        450        460        470        480 
SSSLFDAHVK KEEEKWADIM SMDGTENAYV LHKELGEWMT ANVTVVRHND KLLKTDDKIQ 

       490        500        510        520        530        540 
ELMERFKKIN INDTTKWSNQ GAMFTRQFSN MLQLARVITL GAYNRNESRG AHYKPDYPER 

       550        560        570        580 
NDDEWLKTTM AKHVSPYEAP EFEYQDVDVS LITPRKRDYS KKKVAK 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex."
Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.
J. Bacteriol. 169:864-873(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / PY79.
[2]"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 82; 100; 390 AND 441.
[5]"Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex."
Magnusson K., Philips M.K., Guest J.R., Rutberg L.
J. Bacteriol. 166:1067-1071(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
Strain: 168 / PY79.
[6]"Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme."
Maguire J.J., Magnusson K., Hederstedt L.
Biochemistry 25:5202-5208(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTANT ASP-48.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13470 Genomic DNA. Translation: AAA22746.1.
Z75208 Genomic DNA. Translation: CAA99547.1.
AL009126 Genomic DNA. Translation: CAB14804.2.
PIRA27763.
RefSeqNP_390722.2. NC_000964.3.

3D structure databases

ProteinModelPortalP08065.
SMRP08065. Positions 1-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08065. 1 interaction.
MINTMINT-8366463.
STRING224308.BSU28440.

Proteomic databases

PaxDbP08065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14804; CAB14804; BSU28440.
GeneID937600.
KEGGbsu:BSU28440.
PATRIC18977572. VBIBacSub10457_2974.

Organism-specific databases

GenoListBSU28440. [Micado]

Phylogenomic databases

eggNOGCOG1053.
HOGENOMHOG000160477.
KOK00239.
OrthoDBEOG6F55FG.
PhylomeDBP08065.

Enzyme and pathway databases

BioCycBSUB:BSU28440-MONOMER.
UniPathwayUPA00223; UER01005.

Family and domain databases

Gene3D1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProIPR003953. FAD_bind_dom.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR011280. Succ_DH/Fum_Rdt_flav_su.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsTIGR01811. sdhA_Bsu. 1 hit.
PROSITEPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSDHA_BACSU
AccessionPrimary (citable) accession number: P08065
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList