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P08065

- SDHA_BACSU

UniProt

P08065 - SDHA_BACSU

Protein

Succinate dehydrogenase flavoprotein subunit

Gene

sdhA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 4 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei236 – 2361SubstrateBy similarity
    Binding sitei250 – 2501SubstrateBy similarity
    Active sitei285 – 2851Proton acceptorBy similarity
    Binding sitei352 – 3521SubstrateBy similarity
    Binding sitei376 – 3761FADBy similarity
    Binding sitei386 – 3861SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156FADBy similarity
    Nucleotide bindingi33 – 4816FADBy similarityAdd
    BLAST
    Nucleotide bindingi391 – 3922FADBy similarity

    GO - Molecular functioni

    1. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciBSUB:BSU28440-MONOMER.
    UniPathwayiUPA00223; UER01005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase flavoprotein subunit (EC:1.3.5.1)
    Gene namesi
    Name:sdhA
    Synonyms:citF
    Ordered Locus Names:BSU28440
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU28440. [Micado]

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 586585Succinate dehydrogenase flavoprotein subunitPRO_0000158650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411Tele-8alpha-FAD histidineBy similarity

    Proteomic databases

    PaxDbiP08065.

    Interactioni

    Subunit structurei

    In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

    Protein-protein interaction databases

    IntActiP08065. 1 interaction.
    MINTiMINT-8366463.
    STRINGi224308.BSU28440.

    Structurei

    3D structure databases

    ProteinModelPortaliP08065.
    SMRiP08065. Positions 1-575.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1053.
    HOGENOMiHOG000160477.
    KOiK00239.
    OrthoDBiEOG6F55FG.
    PhylomeDBiP08065.

    Family and domain databases

    Gene3Di1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD_bind_dom.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR011280. Succ_DH/Fum_Rdt_flav_su.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    SUPFAMiSSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsiTIGR01811. sdhA_Bsu. 1 hit.
    PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08065-1 [UniParc]FASTAAdd to Basket

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    MSQSSIIVVG GGLAGLMATI KAAESGMAVK LFSIVPVKRS HSVCAQGGIN    50
    GAVNTKGEGD SPWEHFDDTV YGGDFLANQP PVKAMCEAAP SIIHLLDRMG 100
    VMFNRTPEGL LDFRRFGGTQ HHRTAYAGAT TGQQLLYALD EQVRRYEVAG 150
    LVTKYEGWEF LGAVLDDDRT CRGIVAQNLT NMQIESFRSD AVIMATGGPG 200
    IIFGKSTNSM INTGSAASIV YQQGAYYANG EFIQIHPTAI PGDDKLRLMS 250
    ESARGEGGRV WTYKDGKPWY FLEEKYPAYG NLVPRDIATR EIFDVCVNQK 300
    LGINGENMVY LDLSHKDPKE LDIKLGGIIE IYEKFMGDDP RKLPMKIFPA 350
    VHYSMGGLWV DYDQMTNIPG LFAAGECDYS MHGGNRLGAN SLLSAIYGGM 400
    VAGPNAVKYV NGLESSAEDM SSSLFDAHVK KEEEKWADIM SMDGTENAYV 450
    LHKELGEWMT ANVTVVRHND KLLKTDDKIQ ELMERFKKIN INDTTKWSNQ 500
    GAMFTRQFSN MLQLARVITL GAYNRNESRG AHYKPDYPER NDDEWLKTTM 550
    AKHVSPYEAP EFEYQDVDVS LITPRKRDYS KKKVAK 586
    Length:586
    Mass (Da):65,152
    Last modified:July 7, 2009 - v4
    Checksum:iF2327C2B383DE671
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821V → L in AAA22746. (PubMed:3027051)Curated
    Sequence conflicti82 – 821V → L in CAA99547. (PubMed:8969504)Curated
    Sequence conflicti100 – 1001G → A in AAA22746. (PubMed:3027051)Curated
    Sequence conflicti100 – 1001G → A in CAA99547. (PubMed:8969504)Curated
    Sequence conflicti388 – 3903GAN → ERT in AAA22746. (PubMed:3027051)Curated
    Sequence conflicti388 – 3903GAN → ERT in CAA99547. (PubMed:8969504)Curated
    Sequence conflicti441 – 4411S → I in AAA22746. (PubMed:3027051)Curated
    Sequence conflicti441 – 4411S → I in CAA99547. (PubMed:8969504)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti48 – 481G → D in a defective mutant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13470 Genomic DNA. Translation: AAA22746.1.
    Z75208 Genomic DNA. Translation: CAA99547.1.
    AL009126 Genomic DNA. Translation: CAB14804.2.
    PIRiA27763.
    RefSeqiNP_390722.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14804; CAB14804; BSU28440.
    GeneIDi937600.
    KEGGibsu:BSU28440.
    PATRICi18977572. VBIBacSub10457_2974.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13470 Genomic DNA. Translation: AAA22746.1 .
    Z75208 Genomic DNA. Translation: CAA99547.1 .
    AL009126 Genomic DNA. Translation: CAB14804.2 .
    PIRi A27763.
    RefSeqi NP_390722.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P08065.
    SMRi P08065. Positions 1-575.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08065. 1 interaction.
    MINTi MINT-8366463.
    STRINGi 224308.BSU28440.

    Proteomic databases

    PaxDbi P08065.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14804 ; CAB14804 ; BSU28440 .
    GeneIDi 937600.
    KEGGi bsu:BSU28440.
    PATRICi 18977572. VBIBacSub10457_2974.

    Organism-specific databases

    GenoListi BSU28440. [Micado ]

    Phylogenomic databases

    eggNOGi COG1053.
    HOGENOMi HOG000160477.
    KOi K00239.
    OrthoDBi EOG6F55FG.
    PhylomeDBi P08065.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01005 .
    BioCyci BSUB:BSU28440-MONOMER.

    Family and domain databases

    Gene3Di 1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProi IPR003953. FAD_bind_dom.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR011280. Succ_DH/Fum_Rdt_flav_su.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    SUPFAMi SSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsi TIGR01811. sdhA_Bsu. 1 hit.
    PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex."
      Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.
      J. Bacteriol. 169:864-873(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / PY79.
    2. "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
      Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
      Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 82; 100; 390 AND 441.
    5. "Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex."
      Magnusson K., Philips M.K., Guest J.R., Rutberg L.
      J. Bacteriol. 166:1067-1071(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
      Strain: 168 / PY79.
    6. "Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme."
      Maguire J.J., Magnusson K., Hederstedt L.
      Biochemistry 25:5202-5208(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTANT ASP-48.

    Entry informationi

    Entry nameiSDHA_BACSU
    AccessioniPrimary (citable) accession number: P08065
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 126 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3