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P08065

- SDHA_BACSU

UniProt

P08065 - SDHA_BACSU

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Protein

Succinate dehydrogenase flavoprotein subunit

Gene

sdhA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei236 – 2361SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Active sitei285 – 2851Proton acceptorBy similarity
Binding sitei352 – 3521SubstrateBy similarity
Binding sitei376 – 3761FADBy similarity
Binding sitei386 – 3861SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADBy similarity
Nucleotide bindingi33 – 4816FADBy similarityAdd
BLAST
Nucleotide bindingi391 – 3922FADBy similarity

GO - Molecular functioni

  1. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciBSUB:BSU28440-MONOMER.
UniPathwayiUPA00223; UER01005.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase flavoprotein subunit (EC:1.3.5.1)
Gene namesi
Name:sdhA
Synonyms:citF
Ordered Locus Names:BSU28440
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU28440. [Micado]

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 586585Succinate dehydrogenase flavoprotein subunitPRO_0000158650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411Tele-8alpha-FAD histidineBy similarity

Proteomic databases

PaxDbiP08065.

Interactioni

Subunit structurei

In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

Protein-protein interaction databases

IntActiP08065. 1 interaction.
MINTiMINT-8366463.
STRINGi224308.BSU28440.

Structurei

3D structure databases

ProteinModelPortaliP08065.
SMRiP08065. Positions 1-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1053.
HOGENOMiHOG000160477.
InParanoidiP08065.
KOiK00239.
OrthoDBiEOG6F55FG.
PhylomeDBiP08065.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR011280. Succ_DH/Fum_Rdt_flav_su.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01811. sdhA_Bsu. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08065 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQSSIIVVG GGLAGLMATI KAAESGMAVK LFSIVPVKRS HSVCAQGGIN
60 70 80 90 100
GAVNTKGEGD SPWEHFDDTV YGGDFLANQP PVKAMCEAAP SIIHLLDRMG
110 120 130 140 150
VMFNRTPEGL LDFRRFGGTQ HHRTAYAGAT TGQQLLYALD EQVRRYEVAG
160 170 180 190 200
LVTKYEGWEF LGAVLDDDRT CRGIVAQNLT NMQIESFRSD AVIMATGGPG
210 220 230 240 250
IIFGKSTNSM INTGSAASIV YQQGAYYANG EFIQIHPTAI PGDDKLRLMS
260 270 280 290 300
ESARGEGGRV WTYKDGKPWY FLEEKYPAYG NLVPRDIATR EIFDVCVNQK
310 320 330 340 350
LGINGENMVY LDLSHKDPKE LDIKLGGIIE IYEKFMGDDP RKLPMKIFPA
360 370 380 390 400
VHYSMGGLWV DYDQMTNIPG LFAAGECDYS MHGGNRLGAN SLLSAIYGGM
410 420 430 440 450
VAGPNAVKYV NGLESSAEDM SSSLFDAHVK KEEEKWADIM SMDGTENAYV
460 470 480 490 500
LHKELGEWMT ANVTVVRHND KLLKTDDKIQ ELMERFKKIN INDTTKWSNQ
510 520 530 540 550
GAMFTRQFSN MLQLARVITL GAYNRNESRG AHYKPDYPER NDDEWLKTTM
560 570 580
AKHVSPYEAP EFEYQDVDVS LITPRKRDYS KKKVAK
Length:586
Mass (Da):65,152
Last modified:July 7, 2009 - v4
Checksum:iF2327C2B383DE671
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821V → L in AAA22746. (PubMed:3027051)Curated
Sequence conflicti82 – 821V → L in CAA99547. (PubMed:8969504)Curated
Sequence conflicti100 – 1001G → A in AAA22746. (PubMed:3027051)Curated
Sequence conflicti100 – 1001G → A in CAA99547. (PubMed:8969504)Curated
Sequence conflicti388 – 3903GAN → ERT in AAA22746. (PubMed:3027051)Curated
Sequence conflicti388 – 3903GAN → ERT in CAA99547. (PubMed:8969504)Curated
Sequence conflicti441 – 4411S → I in AAA22746. (PubMed:3027051)Curated
Sequence conflicti441 – 4411S → I in CAA99547. (PubMed:8969504)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti48 – 481G → D in a defective mutant.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13470 Genomic DNA. Translation: AAA22746.1.
Z75208 Genomic DNA. Translation: CAA99547.1.
AL009126 Genomic DNA. Translation: CAB14804.2.
PIRiA27763.
RefSeqiNP_390722.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14804; CAB14804; BSU28440.
GeneIDi937600.
KEGGibsu:BSU28440.
PATRICi18977572. VBIBacSub10457_2974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13470 Genomic DNA. Translation: AAA22746.1 .
Z75208 Genomic DNA. Translation: CAA99547.1 .
AL009126 Genomic DNA. Translation: CAB14804.2 .
PIRi A27763.
RefSeqi NP_390722.2. NC_000964.3.

3D structure databases

ProteinModelPortali P08065.
SMRi P08065. Positions 1-575.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08065. 1 interaction.
MINTi MINT-8366463.
STRINGi 224308.BSU28440.

Proteomic databases

PaxDbi P08065.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14804 ; CAB14804 ; BSU28440 .
GeneIDi 937600.
KEGGi bsu:BSU28440.
PATRICi 18977572. VBIBacSub10457_2974.

Organism-specific databases

GenoListi BSU28440. [Micado ]

Phylogenomic databases

eggNOGi COG1053.
HOGENOMi HOG000160477.
InParanoidi P08065.
KOi K00239.
OrthoDBi EOG6F55FG.
PhylomeDBi P08065.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01005 .
BioCyci BSUB:BSU28440-MONOMER.

Family and domain databases

Gene3Di 1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProi IPR003953. FAD_bind_dom.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR011280. Succ_DH/Fum_Rdt_flav_su.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view ]
Pfami PF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsi TIGR01811. sdhA_Bsu. 1 hit.
PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex."
    Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.
    J. Bacteriol. 169:864-873(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / PY79.
  2. "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
    Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
    Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 82; 100; 390 AND 441.
  5. "Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex."
    Magnusson K., Philips M.K., Guest J.R., Rutberg L.
    J. Bacteriol. 166:1067-1071(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
    Strain: 168 / PY79.
  6. "Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme."
    Maguire J.J., Magnusson K., Hederstedt L.
    Biochemistry 25:5202-5208(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT ASP-48.

Entry informationi

Entry nameiSDHA_BACSU
AccessioniPrimary (citable) accession number: P08065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 7, 2009
Last modified: October 29, 2014
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3