Reviewed, UniProtKB/Swiss-Prot P08065 (DHSA_BACSU)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:	sdhA
Synonyms:	citF
Ordered Locus Names:	BSU28440

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 586	585	Succinate dehydrogenase flavoprotein subunit		PRO_0000158650
Regions
	Nucleotide binding	33 – 48	16	FAD By similarity
Sites
	Active site	285	1	Proton acceptor By similarity
	Binding site	236	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	352	1	Substrate By similarity
	Binding site	386	1	Substrate By similarity
Amino acid modifications
	Modified residue	41	1	Tele-8alpha-FAD histidine By similarity
Natural variations
	Natural variant	48	1	G → D in a defective mutant. Ref.6
Experimental info
	Sequence conflict	82	1	V → L in AAA22746. Ref.1
	Sequence conflict	82	1	V → L in CAA99547. Ref.2
	Sequence conflict	100	1	G → A in AAA22746. Ref.1
	Sequence conflict	100	1	G → A in CAA99547. Ref.2
	Sequence conflict	388 – 390	3	GAN → ERT in AAA22746. Ref.1
	Sequence conflict	388 – 390	3	GAN → ERT in CAA99547. Ref.2
	Sequence conflict	441	1	S → I in AAA22746. Ref.1
	Sequence conflict	441	1	S → I in CAA99547. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08065-1 [UniParc]. Last modified July 7, 2009. Version 4. Checksum: F2327C2B383DE671	FASTA	586	65,152
	10 20 30 40 50 60 MSQSSIIVVG GGLAGLMATI KAAESGMAVK LFSIVPVKRS HSVCAQGGIN GAVNTKGEGD 70 80 90 100 110 120 SPWEHFDDTV YGGDFLANQP PVKAMCEAAP SIIHLLDRMG VMFNRTPEGL LDFRRFGGTQ 130 140 150 160 170 180 HHRTAYAGAT TGQQLLYALD EQVRRYEVAG LVTKYEGWEF LGAVLDDDRT CRGIVAQNLT 190 200 210 220 230 240 NMQIESFRSD AVIMATGGPG IIFGKSTNSM INTGSAASIV YQQGAYYANG EFIQIHPTAI 250 260 270 280 290 300 PGDDKLRLMS ESARGEGGRV WTYKDGKPWY FLEEKYPAYG NLVPRDIATR EIFDVCVNQK 310 320 330 340 350 360 LGINGENMVY LDLSHKDPKE LDIKLGGIIE IYEKFMGDDP RKLPMKIFPA VHYSMGGLWV 370 380 390 400 410 420 DYDQMTNIPG LFAAGECDYS MHGGNRLGAN SLLSAIYGGM VAGPNAVKYV NGLESSAEDM 430 440 450 460 470 480 SSSLFDAHVK KEEEKWADIM SMDGTENAYV LHKELGEWMT ANVTVVRHND KLLKTDDKIQ 490 500 510 520 530 540 ELMERFKKIN INDTTKWSNQ GAMFTRQFSN MLQLARVITL GAYNRNESRG AHYKPDYPER 550 560 570 580 NDDEWLKTTM AKHVSPYEAP EFEYQDVDVS LITPRKRDYS KKKVAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex." Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R. J. Bacteriol. 169:864-873(1987) [PubMed: 3027051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / PY79.
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 82; 100; 390 AND 441.
[5]	"Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex." Magnusson K., Philips M.K., Guest J.R., Rutberg L. J. Bacteriol. 166:1067-1071(1986) [PubMed: 3086287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31. Strain: 168 / PY79.
[6]	"Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme." Maguire J.J., Magnusson K., Hederstedt L. Biochemistry 25:5202-5208(1986) [PubMed: 3021212] [Abstract] Cited for: MUTANT ASP-48.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M13470 Genomic DNA. Translation: AAA22746.1. Z75208 Genomic DNA. Translation: CAA99547.1. AL009126 Genomic DNA. Translation: CAB14804.2.
PIR	A27763.
RefSeq	NP_390722.2.
3D structure databases
HSSP	HSSP built from PDB template 1D4D based on UniProtKB P83223.
ModBase	Search...
Genome annotation databases
GeneID	937600.
GenomeReviews	Gene locus BSU28440 in contig AL009126_GR.
KEGG	bsu:BSU28440.
NMPDR	fig|224308.1.peg.2847.
Organism-specific databases
SubtiList	BG10352. sdhA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P08065.
OMA	FGKSTNS.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2840-MON.
BRENDA	1.3.99.1. 150.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011280. Succ_DH/Fum_Rdt_flav_su. [Graphical view]
PANTHER	PTHR11632:SF4. SDH_A_Bsu. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01811. sdhA_Bsu. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_BACSU

Accession

Primary (citable) accession number: P08065

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 7, 2009
Last modified:	November 3, 2009
This is version 91 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)