Reviewed,
UniProtKB/Swiss-Prot P08065 (DHSA_BACSU)
Last modified
November 3, 2009.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Succinate dehydrogenase flavoprotein subunit EC=1.3.99.1 | ||||||
| Gene names |
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| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 586 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | Succinate + acceptor = fumarate + reduced acceptor. |
| Pathway | |
| Subunit structure | In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558. |
| Subcellular location | Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. |
| Sequence similarities | Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Electron transport Transport Tricarboxylic acid cycle |
| Cellular component | Cell membrane Membrane |
| Ligand | FAD Flavoprotein |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transportInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 586 | 585 | Succinate dehydrogenase flavoprotein subunit | PRO_0000158650 | |||||
Regions | |||||||||
| Nucleotide binding | 33 – 48 | 16 | FAD By similarity | ||||||
Sites | |||||||||
| Active site | 285 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 236 | 1 | Substrate By similarity | ||||||
| Binding site | 250 | 1 | Substrate By similarity | ||||||
| Binding site | 352 | 1 | Substrate By similarity | ||||||
| Binding site | 386 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 41 | 1 | Tele-8alpha-FAD histidine By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 48 | 1 | G → D in a defective mutant. Ref.6 | ||||||
Experimental info | |||||||||
| Sequence conflict | 82 | 1 | V → L in AAA22746. Ref.1 | ||||||
| Sequence conflict | 82 | 1 | V → L in CAA99547. Ref.2 | ||||||
| Sequence conflict | 100 | 1 | G → A in AAA22746. Ref.1 | ||||||
| Sequence conflict | 100 | 1 | G → A in CAA99547. Ref.2 | ||||||
| Sequence conflict | 388 – 390 | 3 | GAN → ERT in AAA22746. Ref.1 | ||||||
| Sequence conflict | 388 – 390 | 3 | GAN → ERT in CAA99547. Ref.2 | ||||||
| Sequence conflict | 441 | 1 | S → I in AAA22746. Ref.1 | ||||||
| Sequence conflict | 441 | 1 | S → I in CAA99547. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex." Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R. J. Bacteriol. 169:864-873(1987) [PubMed: 3027051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / PY79. |
| [2] | "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [3] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [4] | "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 82; 100; 390 AND 441. |
| [5] | "Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex." Magnusson K., Philips M.K., Guest J.R., Rutberg L. J. Bacteriol. 166:1067-1071(1986) [PubMed: 3086287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31. Strain: 168 / PY79. |
| [6] | "Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme." Maguire J.J., Magnusson K., Hederstedt L. Biochemistry 25:5202-5208(1986) [PubMed: 3021212] [Abstract] Cited for: MUTANT ASP-48. |
Cross-references
Sequence databases | |
|---|---|
| M13470 Genomic DNA. Translation: AAA22746.1. Z75208 Genomic DNA. Translation: CAA99547.1. AL009126 Genomic DNA. Translation: CAB14804.2. | |
| PIR | A27763. |
| RefSeq | NP_390722.2. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1D4D based on UniProtKB P83223. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 937600. |
| GenomeReviews | Gene locus BSU28440 in contig AL009126_GR. |
| KEGG | bsu:BSU28440. |
| NMPDR | fig|224308.1.peg.2847. |
Organism-specific databases | |
| SubtiList | BG10352. sdhA. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P08065. |
| OMA | FGKSTNS. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU2840-MON. |
| BRENDA | 1.3.99.1. 150. |
Family and domain databases | |
| InterPro | IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011280. Succ_DH/Fum_Rdt_flav_su. [Graphical view] |
| PANTHER | PTHR11632:SF4. SDH_A_Bsu. 1 hit. |
| Pfam | PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view] |
| PRINTS | PR00368. FADPNR. |
| TIGRFAMs | TIGR01811. sdhA_Bsu. 1 hit. |
| PROSITE | PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DHSA_BACSU | ||||||||
| Accession | Primary (citable) accession number: P08065 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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