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P08059

- G6PI_PIG

UniProt

P08059 - G6PI_PIG

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Protein

Glucose-6-phosphate isomerase

Gene

GPI

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei358 – 3581Proton donorBy similarity
Active sitei389 – 3891By similarity
Active sitei519 – 5191By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. gluconeogenesis Source: UniProtKB-KW
  3. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Angiogenesis, Gluconeogenesis, Glycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Autocrine motility factor
Short name:
AMF
Neuroleukin
Short name:
NLK
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:GPI
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 558557Glucose-6-phosphate isomerasePRO_0000180540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei12 – 121N6-acetyllysineBy similarity
Modified residuei109 – 1091PhosphothreonineBy similarity
Modified residuei142 – 1421N6-acetyllysineBy similarity
Modified residuei185 – 1851Phosphoserine; by CK2By similarity
Modified residuei454 – 4541N6-malonyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP08059.
PRIDEiP08059.

Interactioni

Subunit structurei

Homodimer in the catalytically active form, monomer in the secreted form.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000003094.

Structurei

Secondary structure

1
558
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64Combined sources
Helixi8 – 2013Combined sources
Helixi21 – 233Combined sources
Helixi26 – 327Combined sources
Helixi36 – 394Combined sources
Beta strandi40 – 456Combined sources
Beta strandi50 – 545Combined sources
Helixi62 – 7413Combined sources
Helixi77 – 8610Combined sources
Turni92 – 954Combined sources
Helixi100 – 1034Combined sources
Beta strandi116 – 1183Combined sources
Helixi119 – 13820Combined sources
Beta strandi151 – 1555Combined sources
Helixi159 – 17012Combined sources
Helixi172 – 1754Combined sources
Beta strandi180 – 1845Combined sources
Helixi189 – 1968Combined sources
Helixi201 – 2033Combined sources
Beta strandi204 – 2096Combined sources
Beta strandi211 – 2133Combined sources
Helixi216 – 23318Combined sources
Helixi236 – 2416Combined sources
Beta strandi243 – 2486Combined sources
Helixi250 – 2556Combined sources
Helixi260 – 2623Combined sources
Beta strandi263 – 2653Combined sources
Helixi272 – 2743Combined sources
Turni276 – 2783Combined sources
Helixi279 – 2813Combined sources
Helixi282 – 2887Combined sources
Helixi290 – 30920Combined sources
Helixi312 – 3143Combined sources
Helixi316 – 32914Combined sources
Beta strandi335 – 3417Combined sources
Helixi343 – 3453Combined sources
Helixi348 – 36013Combined sources
Beta strandi378 – 3803Combined sources
Helixi386 – 3894Combined sources
Helixi392 – 3976Combined sources
Beta strandi398 – 4003Combined sources
Beta strandi404 – 4118Combined sources
Helixi416 – 43823Combined sources
Helixi442 – 45110Combined sources
Helixi456 – 4594Combined sources
Turni460 – 4623Combined sources
Helixi463 – 4664Combined sources
Beta strandi474 – 4818Combined sources
Helixi484 – 50522Combined sources
Helixi513 – 5153Combined sources
Helixi516 – 52813Combined sources
Beta strandi530 – 5334Combined sources
Helixi540 – 55415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZDX-ray2.50A2-558[»]
1GZVX-ray3.51A2-558[»]
ProteinModelPortaliP08059.
SMRiP08059. Positions 2-555.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08059.

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiCOG0166.
HOGENOMiHOG000261370.
HOVERGENiHBG002877.
InParanoidiP08059.
KOiK01810.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08059-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALTQNPQF KKLQTWYHEH RSDLNLRRLF EGDKDRFNHF SLNLNTNHGR
60 70 80 90 100
ILLDYSKNLV TEAVMQMLVD LAKSRGVEAA RERMFNGEKI NFTEDRAVLH
110 120 130 140 150
VALRNRSNTP ILVDGKDVMP EVNRVLEKMK SFCKRVRSGE WKGYSGKSIT
160 170 180 190 200
DVINIGIGGS DLGPLMVTEA LKPYSAEGPR VWFVSNIDGT HIAKTLATLN
210 220 230 240 250
PESSLFIIAS KTFTTQETIT NAETAKEWFL QSAKDPSAVA KHFVALSTNT
260 270 280 290 300
TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA
310 320 330 340 350
HWMDQHFRTT PLEKNAPVLL ALLGIWYINF FGCETHAMLP YDQYLHRFAA
360 370 380 390 400
YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK
410 420 430 440 450
MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMKGK STEEARKELQ
460 470 480 490 500
AAGKSPEDFE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV
510 520 530 540 550
QGVIWDINSF DQWGVELGKQ LAKKIEPELD GSSPVTSHDS STNGLINFIK

QEREARSQ
Length:558
Mass (Da):63,126
Last modified:January 23, 2007 - v3
Checksum:i84DA1B15CF0C702F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1572Missing in CAA82246. (PubMed:7821788)Curated
Sequence conflicti344 – 3441Y → H in AAA31048. (PubMed:2889410)Curated
Sequence conflicti347 – 39852RFAAY…LIHQG → ALLPTSSRVTWSPTGSTSPS PAPVWTTRRAPLCGGSQGPM ASMPSTSSS in CAA82246. (PubMed:7821788)CuratedAdd
BLAST
Sequence conflicti481 – 4822KL → NV in CAA82246. (PubMed:7821788)Curated
Sequence conflicti550 – 5501K → M in CAA82246. (PubMed:7821788)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07382 mRNA. Translation: CAA30295.1.
Z28396
, Z28397, Z28398, Z28399, Z28400, Z28401, Z28402, Z28403, Z28404 Genomic DNA. Translation: CAA82246.1.
X53719 mRNA. Translation: CAA37755.1.
M54975 mRNA. Translation: AAA31048.1.
PIRiI47142.
S00895. NUPG.
RefSeqiNP_999495.1. NM_214330.1.
UniGeneiSsc.81817.

Genome annotation databases

GeneIDi397602.
KEGGissc:397602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07382 mRNA. Translation: CAA30295.1 .
Z28396
, Z28397 , Z28398 , Z28399 , Z28400 , Z28401 , Z28402 , Z28403 , Z28404 Genomic DNA. Translation: CAA82246.1 .
X53719 mRNA. Translation: CAA37755.1 .
M54975 mRNA. Translation: AAA31048.1 .
PIRi I47142.
S00895. NUPG.
RefSeqi NP_999495.1. NM_214330.1.
UniGenei Ssc.81817.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GZD X-ray 2.50 A 2-558 [» ]
1GZV X-ray 3.51 A 2-558 [» ]
ProteinModelPortali P08059.
SMRi P08059. Positions 2-555.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000003094.

Proteomic databases

PaxDbi P08059.
PRIDEi P08059.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397602.
KEGGi ssc:397602.

Organism-specific databases

CTDi 2821.

Phylogenomic databases

eggNOGi COG0166.
HOGENOMi HOG000261370.
HOVERGENi HBG002877.
InParanoidi P08059.
KOi K01810.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00181 .

Miscellaneous databases

EvolutionaryTracei P08059.

Family and domain databases

Gene3Di 1.10.1390.10. 1 hit.
HAMAPi MF_00473. G6P_isomerase.
InterProi IPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view ]
PANTHERi PTHR11469. PTHR11469. 1 hit.
Pfami PF00342. PGI. 1 hit.
[Graphical view ]
PRINTSi PR00662. G6PISOMERASE.
PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase."
    Chaput M., Claes V., Portetelle D., Clutdts I., Cravador A., Burny A., Gras H., Tartar A.
    Nature 332:454-455(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Burny A.
    Submitted (AUG-1988) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Structure of the gene encoding pig phosphoglucose isomerase."
    Claes V., Kettmann R., Burny A.
    Gene 150:235-241(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence analysis of the pig phosphoglucose isomerase gene promoter region."
    Claes V., Taquet A.N., Kettmann R., Burny A.
    Biochim. Biophys. Acta 1087:339-340(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-17.
    Strain: Belgian Landrace.
  5. "A partial cDNA clone for porcine glucosephosphate isomerase: isolation, characterization and use in detection of restriction fragment length polymorphisms."
    Davies W., Harbitz I., Hauge J.G.
    Anim. Genet. 18:233-240(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-357.
  6. "Three-dimensional structure of pig muscle phosphoglucose isomerase at 6-A resolution."
    Muirhead H., Shaw P.J.
    J. Mol. Biol. 89:195-203(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (6.0 ANGSTROMS).
  7. Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), PROTEIN SEQUENCE OF 554-558.
  8. "Crystal structure of phosphoglucose isomerase from pig muscle and its complex with 5-phosphoarabinonate."
    Davies C., Muirhead H.
    Proteins 49:577-579(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) ALONE AND IN COMPLEX WITH 5-PHOSPHOARABINONATE.

Entry informationi

Entry nameiG6PI_PIG
AccessioniPrimary (citable) accession number: P08059
Secondary accession number(s): Q29556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3