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P08059

- G6PI_PIG

UniProt

P08059 - G6PI_PIG

Protein

Glucose-6-phosphate isomerase

Gene

GPI

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons By similarity.By similarity

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei358 – 3581Proton donorBy similarity
    Active sitei389 – 3891By similarity
    Active sitei519 – 5191By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. gluconeogenesis Source: UniProtKB-KW
    3. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Cytokine, Isomerase

    Keywords - Biological processi

    Angiogenesis, Gluconeogenesis, Glycolysis

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00181.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate isomerase (EC:5.3.1.9)
    Short name:
    GPI
    Alternative name(s):
    Autocrine motility factor
    Short name:
    AMF
    Neuroleukin
    Short name:
    NLK
    Phosphoglucose isomerase
    Short name:
    PGI
    Phosphohexose isomerase
    Short name:
    PHI
    Gene namesi
    Name:GPI
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 558557Glucose-6-phosphate isomerasePRO_0000180540Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei12 – 121N6-acetyllysineBy similarity
    Modified residuei109 – 1091PhosphothreonineBy similarity
    Modified residuei142 – 1421N6-acetyllysineBy similarity
    Modified residuei185 – 1851Phosphoserine; by CK2By similarity
    Modified residuei454 – 4541N6-malonyllysineBy similarity

    Post-translational modificationi

    ISGylated.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP08059.
    PRIDEiP08059.

    Interactioni

    Subunit structurei

    Homodimer in the catalytically active form, monomer in the secreted form.1 Publication

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000003094.

    Structurei

    Secondary structure

    1
    558
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 64
    Helixi8 – 2013
    Helixi21 – 233
    Helixi26 – 327
    Helixi36 – 394
    Beta strandi40 – 456
    Beta strandi50 – 545
    Helixi62 – 7413
    Helixi77 – 8610
    Turni92 – 954
    Helixi100 – 1034
    Beta strandi116 – 1183
    Helixi119 – 13820
    Beta strandi151 – 1555
    Helixi159 – 17012
    Helixi172 – 1754
    Beta strandi180 – 1845
    Helixi189 – 1968
    Helixi201 – 2033
    Beta strandi204 – 2096
    Beta strandi211 – 2133
    Helixi216 – 23318
    Helixi236 – 2416
    Beta strandi243 – 2486
    Helixi250 – 2556
    Helixi260 – 2623
    Beta strandi263 – 2653
    Helixi272 – 2743
    Turni276 – 2783
    Helixi279 – 2813
    Helixi282 – 2887
    Helixi290 – 30920
    Helixi312 – 3143
    Helixi316 – 32914
    Beta strandi335 – 3417
    Helixi343 – 3453
    Helixi348 – 36013
    Beta strandi378 – 3803
    Helixi386 – 3894
    Helixi392 – 3976
    Beta strandi398 – 4003
    Beta strandi404 – 4118
    Helixi416 – 43823
    Helixi442 – 45110
    Helixi456 – 4594
    Turni460 – 4623
    Helixi463 – 4664
    Beta strandi474 – 4818
    Helixi484 – 50522
    Helixi513 – 5153
    Helixi516 – 52813
    Beta strandi530 – 5334
    Helixi540 – 55415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GZDX-ray2.50A2-558[»]
    1GZVX-ray3.51A2-558[»]
    ProteinModelPortaliP08059.
    SMRiP08059. Positions 2-555.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08059.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI family.Curated

    Phylogenomic databases

    eggNOGiCOG0166.
    HOGENOMiHOG000261370.
    HOVERGENiHBG002877.
    KOiK01810.

    Family and domain databases

    Gene3Di1.10.1390.10. 1 hit.
    HAMAPiMF_00473. G6P_isomerase.
    InterProiIPR001672. G6P_Isomerase.
    IPR023096. G6P_Isomerase_C.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view]
    PANTHERiPTHR11469. PTHR11469. 1 hit.
    PfamiPF00342. PGI. 1 hit.
    [Graphical view]
    PRINTSiPR00662. G6PISOMERASE.
    PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08059-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAALTQNPQF KKLQTWYHEH RSDLNLRRLF EGDKDRFNHF SLNLNTNHGR    50
    ILLDYSKNLV TEAVMQMLVD LAKSRGVEAA RERMFNGEKI NFTEDRAVLH 100
    VALRNRSNTP ILVDGKDVMP EVNRVLEKMK SFCKRVRSGE WKGYSGKSIT 150
    DVINIGIGGS DLGPLMVTEA LKPYSAEGPR VWFVSNIDGT HIAKTLATLN 200
    PESSLFIIAS KTFTTQETIT NAETAKEWFL QSAKDPSAVA KHFVALSTNT 250
    TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA 300
    HWMDQHFRTT PLEKNAPVLL ALLGIWYINF FGCETHAMLP YDQYLHRFAA 350
    YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK 400
    MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMKGK STEEARKELQ 450
    AAGKSPEDFE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV 500
    QGVIWDINSF DQWGVELGKQ LAKKIEPELD GSSPVTSHDS STNGLINFIK 550
    QEREARSQ 558
    Length:558
    Mass (Da):63,126
    Last modified:January 23, 2007 - v3
    Checksum:i84DA1B15CF0C702F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti156 – 1572Missing in CAA82246. (PubMed:7821788)Curated
    Sequence conflicti344 – 3441Y → H in AAA31048. (PubMed:2889410)Curated
    Sequence conflicti347 – 39852RFAAY…LIHQG → ALLPTSSRVTWSPTGSTSPS PAPVWTTRRAPLCGGSQGPM ASMPSTSSS in CAA82246. (PubMed:7821788)CuratedAdd
    BLAST
    Sequence conflicti481 – 4822KL → NV in CAA82246. (PubMed:7821788)Curated
    Sequence conflicti550 – 5501K → M in CAA82246. (PubMed:7821788)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07382 mRNA. Translation: CAA30295.1.
    Z28396
    , Z28397, Z28398, Z28399, Z28400, Z28401, Z28402, Z28403, Z28404 Genomic DNA. Translation: CAA82246.1.
    X53719 mRNA. Translation: CAA37755.1.
    M54975 mRNA. Translation: AAA31048.1.
    PIRiI47142.
    S00895. NUPG.
    RefSeqiNP_999495.1. NM_214330.1.
    UniGeneiSsc.81817.

    Genome annotation databases

    GeneIDi397602.
    KEGGissc:397602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07382 mRNA. Translation: CAA30295.1 .
    Z28396
    , Z28397 , Z28398 , Z28399 , Z28400 , Z28401 , Z28402 , Z28403 , Z28404 Genomic DNA. Translation: CAA82246.1 .
    X53719 mRNA. Translation: CAA37755.1 .
    M54975 mRNA. Translation: AAA31048.1 .
    PIRi I47142.
    S00895. NUPG.
    RefSeqi NP_999495.1. NM_214330.1.
    UniGenei Ssc.81817.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GZD X-ray 2.50 A 2-558 [» ]
    1GZV X-ray 3.51 A 2-558 [» ]
    ProteinModelPortali P08059.
    SMRi P08059. Positions 2-555.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000003094.

    Proteomic databases

    PaxDbi P08059.
    PRIDEi P08059.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397602.
    KEGGi ssc:397602.

    Organism-specific databases

    CTDi 2821.

    Phylogenomic databases

    eggNOGi COG0166.
    HOGENOMi HOG000261370.
    HOVERGENi HBG002877.
    KOi K01810.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00181 .

    Miscellaneous databases

    EvolutionaryTracei P08059.

    Family and domain databases

    Gene3Di 1.10.1390.10. 1 hit.
    HAMAPi MF_00473. G6P_isomerase.
    InterProi IPR001672. G6P_Isomerase.
    IPR023096. G6P_Isomerase_C.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view ]
    PANTHERi PTHR11469. PTHR11469. 1 hit.
    Pfami PF00342. PGI. 1 hit.
    [Graphical view ]
    PRINTSi PR00662. G6PISOMERASE.
    PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase."
      Chaput M., Claes V., Portetelle D., Clutdts I., Cravador A., Burny A., Gras H., Tartar A.
      Nature 332:454-455(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Burny A.
      Submitted (AUG-1988) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Structure of the gene encoding pig phosphoglucose isomerase."
      Claes V., Kettmann R., Burny A.
      Gene 150:235-241(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Sequence analysis of the pig phosphoglucose isomerase gene promoter region."
      Claes V., Taquet A.N., Kettmann R., Burny A.
      Biochim. Biophys. Acta 1087:339-340(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-17.
      Strain: Belgian Landrace.
    5. "A partial cDNA clone for porcine glucosephosphate isomerase: isolation, characterization and use in detection of restriction fragment length polymorphisms."
      Davies W., Harbitz I., Hauge J.G.
      Anim. Genet. 18:233-240(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-357.
    6. "Three-dimensional structure of pig muscle phosphoglucose isomerase at 6-A resolution."
      Muirhead H., Shaw P.J.
      J. Mol. Biol. 89:195-203(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (6.0 ANGSTROMS).
    7. Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), PROTEIN SEQUENCE OF 554-558.
    8. "Crystal structure of phosphoglucose isomerase from pig muscle and its complex with 5-phosphoarabinonate."
      Davies C., Muirhead H.
      Proteins 49:577-579(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) ALONE AND IN COMPLEX WITH 5-PHOSPHOARABINONATE.

    Entry informationi

    Entry nameiG6PI_PIG
    AccessioniPrimary (citable) accession number: P08059
    Secondary accession number(s): Q29556
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3