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Protein

Troponin I, cardiac muscle

Gene

TNNI3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei82Involved in TNI-TNT interactions1
Sitei99Involved in TNI-TNT interactions1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi139 – 150By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-390522. Striated Muscle Contraction.
R-BTA-5578775. Ion homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:TNNI3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001861492 – 212Troponin I, cardiac muscleAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei5PhosphoserineBy similarity1
Modified residuei24Phosphoserine; by PKA and PKD/PRKD1By similarity1
Modified residuei25Phosphoserine; by PKA and PKD/PRKD1By similarity1
Modified residuei28PhosphotyrosineBy similarity1
Modified residuei33Phosphothreonine; by STK4/MST1By similarity1
Modified residuei44Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei46Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei53Phosphothreonine; by STK4/MST1By similarity1
Modified residuei79PhosphoserineBy similarity1
Modified residuei80PhosphothreonineBy similarity1
Modified residuei145Phosphothreonine; by STK4/MST1By similarity1
Modified residuei152Phosphoserine; by PAK3By similarity1
Modified residuei183PhosphothreonineBy similarity1
Modified residuei201PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at Ser-24 and Ser-25 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-33. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylated at Ser-44 and Ser-46 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP08057.
PRIDEiP08057.

PTM databases

iPTMnetiP08057.

Expressioni

Gene expression databases

BgeeiENSBTAG00000006424.

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP08057. 1 interactor.
STRINGi9913.ENSBTAP00000008425.

Structurei

3D structure databases

ProteinModelPortaliP08057.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 81Involved in binding TNCAdd BLAST48
Regioni131 – 151Involved in binding TNC and actinAdd BLAST21

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP08057.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG091G0NOD.
TreeFamiTF313374.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRSGGSTA GDTVPAPPPV RRRSSANYRA YATEPHAKKK SKISASRKLQ
60 70 80 90 100
LKTLMLQIAK QELEREAEER RGEKGRALST RCQPLELAGL GFAELQDLCR
110 120 130 140 150
QLHARVDKVD EERYDVEAKV TKNITEIADL NQKIFDLRGK FKRPTLRRVR
160 170 180 190 200
ISADAMMQAL LGARAKETLD LRAHLKQVKK EDTEKENREV GDWRKNIDAL
210
SGMEGRKKKF EG
Length:212
Mass (Da):24,054
Last modified:January 23, 2007 - v2
Checksum:iB4B15DCCA7BA2B5E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17P → M AA sequence (PubMed:2967699).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ842179 Genomic DNA. Translation: CAH57016.1.
PIRiA29994.
RefSeqiNP_001035607.1. NM_001040517.1.
UniGeneiBt.11438.

Genome annotation databases

EnsembliENSBTAT00000008425; ENSBTAP00000008425; ENSBTAG00000006424.
GeneIDi511094.
KEGGibta:511094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ842179 Genomic DNA. Translation: CAH57016.1.
PIRiA29994.
RefSeqiNP_001035607.1. NM_001040517.1.
UniGeneiBt.11438.

3D structure databases

ProteinModelPortaliP08057.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08057. 1 interactor.
STRINGi9913.ENSBTAP00000008425.

PTM databases

iPTMnetiP08057.

Proteomic databases

PaxDbiP08057.
PRIDEiP08057.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008425; ENSBTAP00000008425; ENSBTAG00000006424.
GeneIDi511094.
KEGGibta:511094.

Organism-specific databases

CTDi7137.

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP08057.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG091G0NOD.
TreeFamiTF313374.

Enzyme and pathway databases

ReactomeiR-BTA-390522. Striated Muscle Contraction.
R-BTA-5578775. Ion homeostasis.

Gene expression databases

BgeeiENSBTAG00000006424.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNNI3_BOVIN
AccessioniPrimary (citable) accession number: P08057
Secondary accession number(s): Q5ZG22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.