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Protein

Troponin I, cardiac muscle

Gene

TNNI3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei82 – 821Involved in TNI-TNT interactions
Sitei99 – 991Involved in TNI-TNT interactions

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi139 – 15012By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-390522. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:TNNI3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 212211Troponin I, cardiac musclePRO_0000186149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei5 – 51PhosphoserineBy similarity
Modified residuei24 – 241Phosphoserine; by PKA and PKD/PRKD1By similarity
Modified residuei25 – 251Phosphoserine; by PKA and PKD/PRKD1By similarity
Modified residuei28 – 281PhosphotyrosineBy similarity
Modified residuei33 – 331Phosphothreonine; by STK4/MST1By similarity
Modified residuei44 – 441Phosphoserine; by PKC/PRKCEBy similarity
Modified residuei46 – 461Phosphoserine; by PKC/PRKCEBy similarity
Modified residuei53 – 531Phosphothreonine; by STK4/MST1By similarity
Modified residuei79 – 791PhosphoserineBy similarity
Modified residuei80 – 801PhosphothreonineBy similarity
Modified residuei145 – 1451Phosphothreonine; by STK4/MST1By similarity
Modified residuei152 – 1521Phosphoserine; by PAK3By similarity
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei201 – 2011PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser-24 and Ser-25 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-33. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylated at Ser-44 and Ser-46 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP08057.
PRIDEiP08057.

PTM databases

iPTMnetiP08057.

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP08057. 1 interaction.
STRINGi9913.ENSBTAP00000008425.

Structurei

3D structure databases

ProteinModelPortaliP08057.
SMRiP08057. Positions 1-33, 42-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 8148Involved in binding TNCAdd
BLAST
Regioni131 – 15121Involved in binding TNC and actinAdd
BLAST

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP08057.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG71G9WD.
TreeFamiTF313374.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRSGGSTA GDTVPAPPPV RRRSSANYRA YATEPHAKKK SKISASRKLQ
60 70 80 90 100
LKTLMLQIAK QELEREAEER RGEKGRALST RCQPLELAGL GFAELQDLCR
110 120 130 140 150
QLHARVDKVD EERYDVEAKV TKNITEIADL NQKIFDLRGK FKRPTLRRVR
160 170 180 190 200
ISADAMMQAL LGARAKETLD LRAHLKQVKK EDTEKENREV GDWRKNIDAL
210
SGMEGRKKKF EG
Length:212
Mass (Da):24,054
Last modified:January 23, 2007 - v2
Checksum:iB4B15DCCA7BA2B5E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171P → M AA sequence (PubMed:2967699).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ842179 Genomic DNA. Translation: CAH57016.1.
PIRiA29994.
RefSeqiNP_001035607.1. NM_001040517.1.
UniGeneiBt.11438.

Genome annotation databases

EnsembliENSBTAT00000008425; ENSBTAP00000008425; ENSBTAG00000006424.
GeneIDi511094.
KEGGibta:511094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ842179 Genomic DNA. Translation: CAH57016.1.
PIRiA29994.
RefSeqiNP_001035607.1. NM_001040517.1.
UniGeneiBt.11438.

3D structure databases

ProteinModelPortaliP08057.
SMRiP08057. Positions 1-33, 42-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08057. 1 interaction.
STRINGi9913.ENSBTAP00000008425.

PTM databases

iPTMnetiP08057.

Proteomic databases

PaxDbiP08057.
PRIDEiP08057.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008425; ENSBTAP00000008425; ENSBTAG00000006424.
GeneIDi511094.
KEGGibta:511094.

Organism-specific databases

CTDi7137.

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP08057.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG71G9WD.
TreeFamiTF313374.

Enzyme and pathway databases

ReactomeiR-BTA-390522. Striated Muscle Contraction.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Bovine dilated cardiomyopathy: evidence for a major gene on BTA18."
    Guziewicz K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Peripheral blood.
  2. "Amino acid sequence of bovine cardiac troponin I."
    Leszyk J., Dumaswala R., Potter J.D., Collins J.H.
    Biochemistry 27:2821-2827(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-212, ACETYLATION AT ALA-2.
  3. "Pattern of repeating aromatic residues in synexin. Similarity to the cytoplasmic domain of synaptophysin."
    Creutz C.E., Snyder S.L., Husted L.D., Beggerly L.K., Fox J.W.
    Biochem. Biophys. Res. Commun. 152:1298-1303(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-212.

Entry informationi

Entry nameiTNNI3_BOVIN
AccessioniPrimary (citable) accession number: P08057
Secondary accession number(s): Q5ZG22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.