P08056 (RNRH_RHINI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease Rh Short name=RNase Rh EC=3.1.27.1
Organism	Rhizopus niveus
Taxonomic identifier	4844 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Fungi incertae sedis › Early diverging fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Rhizopus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a base non-specific ribonuclease.
Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Sequence similarities	Belongs to the RNase T2 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease T2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Ref.1

Chain

17 – 238

222

Ribonuclease Rh

PRO_0000030964

Sites

Active site

62

1

Active site

121

1

Active site

125

1

Amino acid modifications

Disulfide bond

19 ↔ 36

Ref.4

Disulfide bond

26 ↔ 69

Ref.4

Disulfide bond

35 ↔ 136

Ref.4

Disulfide bond

79 ↔ 128

Ref.4

Disulfide bond

198 ↔ 229

Ref.4

Experimental info

Sequence conflict

82 – 84

3

NRA → SLY in BAA00167. Ref.1

Secondary structure

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238

Helix Strand Turn

Details...

Beta strand

24 – 27

4

Turn

34 – 36

3

Beta strand

42 – 48

7

Beta strand

60 – 67

8

Helix

88 – 95

8

Helix

97 – 106

10

Helix

114 – 124

11

Helix

126 – 128

3

Helix

130 – 132

3

Helix

134 – 136

3

Helix

144 – 159

16

Helix

162 – 167

6

Turn

168 – 170

3

Beta strand

173 – 178

6

Helix

179 – 190

12

Beta strand

195 – 199

5

Beta strand

202 – 213

12

Turn

214 – 216

3

Beta strand

217 – 221

5

Beta strand

231 – 235

5

Sequences

	Sequence		Length	Mass (Da)
	P08056 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: A3BAB811D24334B3	FASTA	238	25,635
	10 20 30 40 50 60 MKAVLALATL IGSTLASSCS STALSCSNSA NSDTCCSPEY GLVVLNMQWA PGYGPDNAFT 70 80 90 100 110 120 LHGLWPDKCS GAYAPSGGCD SNRASSSIAS VIKSKDSSLY NSMLTYWPSN QGNNNVFWSH 130 140 150 160 170 180 EWSKHGTCVS TYDPDCYDNY EEGEDIVDYF QKAMDLRSQY NVYKAFSSNG ITPGGTYTAT 190 200 210 220 230 EMQSAIESYF GAKAKIDCSS GTLSDVALYF YVRGRDTYVI TDALSTGSCS GDVEYPTK

References

[1]	"Primary structure of a base non-specific ribonuclease from Rhizopus niveus." Horiuchi H., Yanai K., Takagi M., Yano K., Wakabayashi E., Sanda A., Mine S., Ohgi K., Irie M. J. Biochem. 103:408-418(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-238. Strain: NBRC 4810 / AS 3.4817.
[2]	"Expression of RNase Rh from Rhizopus niveus in yeast and characterization of the secreted proteins." Ohgi K., Horiuchi H., Watanabe H., Takagi M., Yano K., Irie M. J. Biochem. 109:776-785(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NBRC 4810 / AS 3.4817.
[3]	"Crystal and molecular structure of RNase Rh, a new class of microbial ribonuclease from Rhizopus niveus." Kurihara H., Mitsui Y., Ohgi K., Irie M., Mizuno H., Nakamura K.T. FEBS Lett. 306:189-192(1992) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[4]	"The crystal structure of ribonuclease Rh from Rhizopus niveus at 2.0-A resolution." Kurihara H., Nonaka T., Mitsui Y., Ohgi K., Irie M., Nakamura K.T. J. Mol. Biol. 255:310-320(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D00238 Genomic DNA. Translation: BAA00167.1.
D12476 mRNA. Translation: BAA02042.1.

PIR

JH0367.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BOL	X-ray	2.00	A	17-238	[»]

ProteinModelPortal

P08056.

SMR

P08056. Positions 17-238.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.90.730.10. 1 hit.

InterPro

IPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]

PANTHER

PTHR11240. PTHR11240. 1 hit.

Pfam

PF00445. Ribonuclease_T2. 1 hit.
[Graphical view]

SUPFAM

SSF55895. RNase_T2. 1 hit.

PROSITE

PS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P08056.

Entry information

Entry name

RNRH_RHINI

Accession

Primary (citable) accession number: P08056

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program