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P08056

- RNRH_RHINI

UniProt

P08056 - RNRH_RHINI

Protein

Ribonuclease Rh

Gene
N/A
Organism
Rhizopus niveus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    This is a base non-specific ribonuclease.

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei62 – 621
    Active sitei121 – 1211
    Active sitei125 – 1251

    GO - Molecular functioni

    1. ribonuclease T2 activity Source: UniProtKB-EC
    2. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease Rh (EC:3.1.27.1)
    Short name:
    RNase Rh
    OrganismiRhizopus niveus
    Taxonomic identifieri4844 [NCBI]
    Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeRhizopodaceaeRhizopus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 16161 PublicationAdd
    BLAST
    Chaini17 – 238222Ribonuclease RhPRO_0000030964Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi19 ↔ 361 Publication
    Disulfide bondi26 ↔ 691 Publication
    Disulfide bondi35 ↔ 1361 Publication
    Disulfide bondi79 ↔ 1281 Publication
    Disulfide bondi198 ↔ 2291 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    238
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi24 – 274
    Turni34 – 363
    Beta strandi42 – 487
    Beta strandi60 – 678
    Helixi88 – 958
    Helixi97 – 10610
    Helixi114 – 12411
    Helixi126 – 1283
    Helixi130 – 1323
    Helixi134 – 1363
    Helixi144 – 15916
    Helixi162 – 1676
    Turni168 – 1703
    Beta strandi173 – 1786
    Helixi179 – 19012
    Beta strandi195 – 1995
    Beta strandi202 – 21312
    Turni214 – 2163
    Beta strandi217 – 2215
    Beta strandi231 – 2355

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BOLX-ray2.00A17-238[»]
    ProteinModelPortaliP08056.
    SMRiP08056. Positions 17-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08056.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNase T2 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.90.730.10. 1 hit.
    InterProiIPR001568. RNase_T2-like.
    IPR018188. RNase_T2_AS.
    [Graphical view]
    PANTHERiPTHR11240. PTHR11240. 1 hit.
    PfamiPF00445. Ribonuclease_T2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55895. SSF55895. 1 hit.
    PROSITEiPS00530. RNASE_T2_1. 1 hit.
    PS00531. RNASE_T2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAVLALATL IGSTLASSCS STALSCSNSA NSDTCCSPEY GLVVLNMQWA    50
    PGYGPDNAFT LHGLWPDKCS GAYAPSGGCD SNRASSSIAS VIKSKDSSLY 100
    NSMLTYWPSN QGNNNVFWSH EWSKHGTCVS TYDPDCYDNY EEGEDIVDYF 150
    QKAMDLRSQY NVYKAFSSNG ITPGGTYTAT EMQSAIESYF GAKAKIDCSS 200
    GTLSDVALYF YVRGRDTYVI TDALSTGSCS GDVEYPTK 238
    Length:238
    Mass (Da):25,635
    Last modified:August 1, 1988 - v1
    Checksum:iA3BAB811D24334B3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 843NRA → SLY in BAA00167. (PubMed:3391995)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00238 Genomic DNA. Translation: BAA00167.1.
    D12476 mRNA. Translation: BAA02042.1.
    PIRiJH0367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00238 Genomic DNA. Translation: BAA00167.1 .
    D12476 mRNA. Translation: BAA02042.1 .
    PIRi JH0367.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BOL X-ray 2.00 A 17-238 [» ]
    ProteinModelPortali P08056.
    SMRi P08056. Positions 17-238.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P08056.

    Family and domain databases

    Gene3Di 3.90.730.10. 1 hit.
    InterProi IPR001568. RNase_T2-like.
    IPR018188. RNase_T2_AS.
    [Graphical view ]
    PANTHERi PTHR11240. PTHR11240. 1 hit.
    Pfami PF00445. Ribonuclease_T2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55895. SSF55895. 1 hit.
    PROSITEi PS00530. RNASE_T2_1. 1 hit.
    PS00531. RNASE_T2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a base non-specific ribonuclease from Rhizopus niveus."
      Horiuchi H., Yanai K., Takagi M., Yano K., Wakabayashi E., Sanda A., Mine S., Ohgi K., Irie M.
      J. Biochem. 103:408-418(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-238.
      Strain: NBRC 4810 / AS 3.4817.
    2. "Expression of RNase Rh from Rhizopus niveus in yeast and characterization of the secreted proteins."
      Ohgi K., Horiuchi H., Watanabe H., Takagi M., Yano K., Irie M.
      J. Biochem. 109:776-785(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: NBRC 4810 / AS 3.4817.
    3. "Crystal and molecular structure of RNase Rh, a new class of microbial ribonuclease from Rhizopus niveus."
      Kurihara H., Mitsui Y., Ohgi K., Irie M., Mizuno H., Nakamura K.T.
      FEBS Lett. 306:189-192(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    4. "The crystal structure of ribonuclease Rh from Rhizopus niveus at 2.0-A resolution."
      Kurihara H., Nonaka T., Mitsui Y., Ohgi K., Irie M., Nakamura K.T.
      J. Mol. Biol. 255:310-320(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiRNRH_RHINI
    AccessioniPrimary (citable) accession number: P08056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3