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P08056

- RNRH_RHINI

UniProt

P08056 - RNRH_RHINI

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Protein

Ribonuclease Rh

Gene
N/A
Organism
Rhizopus niveus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This is a base non-specific ribonuclease.

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621
Active sitei121 – 1211
Active sitei125 – 1251

GO - Molecular functioni

  1. ribonuclease T2 activity Source: UniProtKB-EC
  2. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease Rh (EC:3.1.27.1)
Short name:
RNase Rh
OrganismiRhizopus niveus
Taxonomic identifieri4844 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeRhizopodaceaeRhizopus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Chaini17 – 238222Ribonuclease RhPRO_0000030964Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi19 ↔ 361 Publication
Disulfide bondi26 ↔ 691 Publication
Disulfide bondi35 ↔ 1361 Publication
Disulfide bondi79 ↔ 1281 Publication
Disulfide bondi198 ↔ 2291 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
238
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 274Combined sources
Turni34 – 363Combined sources
Beta strandi42 – 487Combined sources
Beta strandi60 – 678Combined sources
Helixi88 – 958Combined sources
Helixi97 – 10610Combined sources
Helixi114 – 12411Combined sources
Helixi126 – 1283Combined sources
Helixi130 – 1323Combined sources
Helixi134 – 1363Combined sources
Helixi144 – 15916Combined sources
Helixi162 – 1676Combined sources
Turni168 – 1703Combined sources
Beta strandi173 – 1786Combined sources
Helixi179 – 19012Combined sources
Beta strandi195 – 1995Combined sources
Beta strandi202 – 21312Combined sources
Turni214 – 2163Combined sources
Beta strandi217 – 2215Combined sources
Beta strandi231 – 2355Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BOLX-ray2.00A17-238[»]
ProteinModelPortaliP08056.
SMRiP08056. Positions 17-238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08056.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase T2 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.90.730.10. 1 hit.
InterProiIPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08056-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAVLALATL IGSTLASSCS STALSCSNSA NSDTCCSPEY GLVVLNMQWA
60 70 80 90 100
PGYGPDNAFT LHGLWPDKCS GAYAPSGGCD SNRASSSIAS VIKSKDSSLY
110 120 130 140 150
NSMLTYWPSN QGNNNVFWSH EWSKHGTCVS TYDPDCYDNY EEGEDIVDYF
160 170 180 190 200
QKAMDLRSQY NVYKAFSSNG ITPGGTYTAT EMQSAIESYF GAKAKIDCSS
210 220 230
GTLSDVALYF YVRGRDTYVI TDALSTGSCS GDVEYPTK
Length:238
Mass (Da):25,635
Last modified:August 1, 1988 - v1
Checksum:iA3BAB811D24334B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 843NRA → SLY in BAA00167. (PubMed:3391995)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00238 Genomic DNA. Translation: BAA00167.1.
D12476 mRNA. Translation: BAA02042.1.
PIRiJH0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00238 Genomic DNA. Translation: BAA00167.1 .
D12476 mRNA. Translation: BAA02042.1 .
PIRi JH0367.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BOL X-ray 2.00 A 17-238 [» ]
ProteinModelPortali P08056.
SMRi P08056. Positions 17-238.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P08056.

Family and domain databases

Gene3Di 3.90.730.10. 1 hit.
InterProi IPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view ]
PANTHERi PTHR11240. PTHR11240. 1 hit.
Pfami PF00445. Ribonuclease_T2. 1 hit.
[Graphical view ]
SUPFAMi SSF55895. SSF55895. 1 hit.
PROSITEi PS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of a base non-specific ribonuclease from Rhizopus niveus."
    Horiuchi H., Yanai K., Takagi M., Yano K., Wakabayashi E., Sanda A., Mine S., Ohgi K., Irie M.
    J. Biochem. 103:408-418(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-238.
    Strain: NBRC 4810 / AS 3.4817.
  2. "Expression of RNase Rh from Rhizopus niveus in yeast and characterization of the secreted proteins."
    Ohgi K., Horiuchi H., Watanabe H., Takagi M., Yano K., Irie M.
    J. Biochem. 109:776-785(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NBRC 4810 / AS 3.4817.
  3. "Crystal and molecular structure of RNase Rh, a new class of microbial ribonuclease from Rhizopus niveus."
    Kurihara H., Mitsui Y., Ohgi K., Irie M., Mizuno H., Nakamura K.T.
    FEBS Lett. 306:189-192(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  4. "The crystal structure of ribonuclease Rh from Rhizopus niveus at 2.0-A resolution."
    Kurihara H., Nonaka T., Mitsui Y., Ohgi K., Irie M., Nakamura K.T.
    J. Mol. Biol. 255:310-320(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiRNRH_RHINI
AccessioniPrimary (citable) accession number: P08056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3