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Protein

Ribonuclease Rh

Gene
N/A
Organism
Rhizopus niveus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This is a base non-specific ribonuclease.

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei621 Publication1
Active sitei1211 Publication1
Active sitei1251 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease Rh (EC:3.1.27.1)
Short name:
RNase Rh
OrganismiRhizopus niveus
Taxonomic identifieri4844 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotinaMucoralesMucorineaeRhizopodaceaeRhizopus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
ChainiPRO_000003096417 – 238Ribonuclease RhAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi19 ↔ 361 Publication
Disulfide bondi26 ↔ 691 Publication
Disulfide bondi35 ↔ 1361 Publication
Disulfide bondi79 ↔ 1281 Publication
Disulfide bondi198 ↔ 2291 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 27Combined sources4
Turni34 – 36Combined sources3
Beta strandi42 – 48Combined sources7
Beta strandi60 – 67Combined sources8
Helixi88 – 95Combined sources8
Helixi97 – 106Combined sources10
Helixi114 – 124Combined sources11
Helixi126 – 128Combined sources3
Helixi130 – 132Combined sources3
Helixi134 – 136Combined sources3
Helixi144 – 159Combined sources16
Helixi162 – 167Combined sources6
Turni168 – 170Combined sources3
Beta strandi173 – 178Combined sources6
Helixi179 – 190Combined sources12
Beta strandi195 – 199Combined sources5
Beta strandi202 – 213Combined sources12
Turni214 – 216Combined sources3
Beta strandi217 – 221Combined sources5
Beta strandi231 – 235Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BOLX-ray2.00A17-238[»]
ProteinModelPortaliP08056.
SMRiP08056.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08056.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase T2 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd01061. RNase_T2_euk. 1 hit.
Gene3Di3.90.730.10. 1 hit.
InterProiIPR033697. Ribonuclease_T2_eukaryotic.
IPR001568. RNase_T2-like.
IPR018188. RNase_T2_His_AS_1.
IPR033130. RNase_T2_His_AS_2.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVLALATL IGSTLASSCS STALSCSNSA NSDTCCSPEY GLVVLNMQWA
60 70 80 90 100
PGYGPDNAFT LHGLWPDKCS GAYAPSGGCD SNRASSSIAS VIKSKDSSLY
110 120 130 140 150
NSMLTYWPSN QGNNNVFWSH EWSKHGTCVS TYDPDCYDNY EEGEDIVDYF
160 170 180 190 200
QKAMDLRSQY NVYKAFSSNG ITPGGTYTAT EMQSAIESYF GAKAKIDCSS
210 220 230
GTLSDVALYF YVRGRDTYVI TDALSTGSCS GDVEYPTK
Length:238
Mass (Da):25,635
Last modified:August 1, 1988 - v1
Checksum:iA3BAB811D24334B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82 – 84NRA → SLY in BAA00167 (PubMed:3391995).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00238 Genomic DNA. Translation: BAA00167.1.
D12476 mRNA. Translation: BAA02042.1.
PIRiJH0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00238 Genomic DNA. Translation: BAA00167.1.
D12476 mRNA. Translation: BAA02042.1.
PIRiJH0367.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BOLX-ray2.00A17-238[»]
ProteinModelPortaliP08056.
SMRiP08056.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08056.

Family and domain databases

CDDicd01061. RNase_T2_euk. 1 hit.
Gene3Di3.90.730.10. 1 hit.
InterProiIPR033697. Ribonuclease_T2_eukaryotic.
IPR001568. RNase_T2-like.
IPR018188. RNase_T2_His_AS_1.
IPR033130. RNase_T2_His_AS_2.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNRH_RHINI
AccessioniPrimary (citable) accession number: P08056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.