Reviewed,
UniProtKB/Swiss-Prot P08050 (CXA1_RAT)
Last modified
June 16, 2009.
Version 98.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Gap junction alpha-1 protein Alternative name(s): Connexin-43 Short name=Cx43 Gap junction 43 kDa heart protein | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 382 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
| Subunit structure | A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150 By similarity. |
| Subcellular location | Cell membrane; Multi-pass membrane protein. Cell junction › gap junction. |
| Post-translational modification | Contains at least one intramolecular disulfide bond. |
| Sequence similarities | Belongs to the connexin family. Alpha-type (group II) subfamily. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| DBN1 | Q16643 | 1 | EBI-476947,EBI-351394 | From a different organism. |
| Dbn1 | Q9QXS6 | 1 | EBI-476947,EBI-445208 | From a different organism. |
| Sgsm3 | Q8VCZ6 | 1 | EBI-476947,EBI-525155 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.5 Ref.6 | |||||||||||||||||||||||
| Chain | 2 – 382 | 381 | Gap junction alpha-1 protein | PRO_0000057804 | ||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||
| Topological domain | 2 – 13 | 12 | Cytoplasmic Probable | |||||||||||||||||||||||
| Transmembrane | 14 – 36 | 23 | Probable | |||||||||||||||||||||||
| Topological domain | 37 – 76 | 40 | Extracellular Probable | |||||||||||||||||||||||
| Transmembrane | 77 – 99 | 23 | Probable | |||||||||||||||||||||||
| Topological domain | 100 – 154 | 55 | Cytoplasmic Probable | |||||||||||||||||||||||
| Transmembrane | 155 – 177 | 23 | Probable | |||||||||||||||||||||||
| Topological domain | 178 – 208 | 31 | Extracellular Probable | |||||||||||||||||||||||
| Transmembrane | 209 – 231 | 23 | Probable | |||||||||||||||||||||||
| Topological domain | 232 – 382 | 151 | Cytoplasmic Probable | |||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||
| Modified residue | 247 | 1 | Phosphotyrosine By similarity | |||||||||||||||||||||||
| Modified residue | 306 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||
| Modified residue | 313 | 1 | Phosphotyrosine By similarity | |||||||||||||||||||||||
| Modified residue | 314 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||
| Modified residue | 326 | 1 | Phosphothreonine By similarity | |||||||||||||||||||||||
| Modified residue | 328 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||
| Modified residue | 330 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||
| Modified residue | 365 | 1 | Phosphoserine Ref.9 | |||||||||||||||||||||||
| Modified residue | 368 | 1 | Phosphoserine Ref.9 | |||||||||||||||||||||||
| Modified residue | 369 | 1 | Phosphoserine Ref.9 | |||||||||||||||||||||||
| Modified residue | 373 | 1 | Phosphoserine Ref.9 | |||||||||||||||||||||||
| Disulfide bond | 54 ↔ 192 | By similarity | ||||||||||||||||||||||||
| Disulfide bond | 187 ↔ 198 | By similarity | ||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||
| Sequence conflict | 2 | 1 | G → A AA sequence Ref.5 | |||||||||||||||||||||||
| Sequence conflict | 16 | 1 | A → T Ref.2 | |||||||||||||||||||||||
| Sequence conflict | 28 | 1 | V → I AA sequence Ref.5 | |||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||
| Turn | 256 – 259 | 4 | ||||||||||||||||||||||||
| Beta strand | 263 – 265 | 3 | ||||||||||||||||||||||||
| Beta strand | 297 – 300 | 4 | ||||||||||||||||||||||||
| Helix | 317 – 325 | 9 | ||||||||||||||||||||||||
| Helix | 342 – 348 | 7 | ||||||||||||||||||||||||
| Turn | 349 – 352 | 4 | ||||||||||||||||||||||||
| Beta strand | 360 – 362 | 3 | ||||||||||||||||||||||||
| Turn | 366 – 370 | 5 | ||||||||||||||||||||||||
| Beta strand | 376 – 380 | 5 | ||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Connexin43: a protein from rat heart homologous to a gap junction protein from liver." Beyer E.C., Paul D.L., Goodenough D.A. J. Cell Biol. 105:2621-2629(1987) [PubMed: 2826492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Molecular cloning of a rat uterine gap junction protein and analysis of gene expression during gestation." Lang L.M., Beyer E.C., Schwartz A.L., Gitlin J.D. Am. J. Physiol. 260:E787-E793(1991) [PubMed: 1852114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Uterus. |
| [3] | Ma L.X., Peng Y.W. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart. |
| [5] | "The Mr 28,000 gap junction proteins from rat heart and liver are different but related." Nicholson B.J., Gros D.B., Kent S.B.H., Hood L.E., Revel J.-P. J. Biol. Chem. 260:6514-6517(1985) [PubMed: 2987225] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-33. Tissue: Heart. |
| [6] | "Affinity purification of a rat-brain junctional protein, connexin 43." Dupont E., el Aoumari A., Fromaget C., Briand J.-C., Gros D. Eur. J. Biochem. 200:263-270(1991) [PubMed: 1652440] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE OF 2-16. Tissue: Brain. |
| [7] | "Connexon integrity is maintained by non-covalent bonds: intramolecular disulfide bonds link the extracellular domains in rat connexin-43." John S.A., Revel J.-P. Biochem. Biophys. Res. Commun. 178:1312-1318(1991) [PubMed: 1651718] [Abstract] Cited for: DISULFIDE BONDS. |
| [8] | "Membrane topology and quaternary structure of cardiac gap junction ion channels." Yeager M., Gilula N.B. J. Mol. Biol. 223:929-948(1992) [PubMed: 1371548] [Abstract] Cited for: TOPOLOGY. |
| [9] | "Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells." Yogo K., Ogawa T., Akiyama M., Ishida N., Takeya T. FEBS Lett. 531:132-136(2002) [PubMed: 12417300] [Abstract] Cited for: PHOSPHORYLATION AT SER-365; SER-368; SER-369 AND SER-373. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X06656 mRNA. Translation: CAA29855.1. AY324140 mRNA. Translation: AAP88589.1. BC081842 mRNA. Translation: AAH81842.1. | |||||||||||||||||||
| IPI | IPI00231746. | ||||||||||||||||||
| PIR | A24047. S00532. | ||||||||||||||||||
| RefSeq | NP_036699.1. | ||||||||||||||||||
| UniGene | Rn.10346 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| |||||||||||||||||||
| DisProt | DP00278. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P08050. 4 interactions. | ||||||||||||||||||
Protein family/group databases | |||||||||||||||||||
| TCDB | 1.A.24.1.1. gap junction-forming connexin family. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P08050. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENSRNOG00000000805. Rattus norvegicus. [Contig view] | ||||||||||||||||||
| GeneID | 24392. | ||||||||||||||||||
| KEGG | rno:24392. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| RGD | 2690. Gja1. | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOVERGEN | P08050. | ||||||||||||||||||
| OMA | P08050. TDGANVD. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| Reactome | REACT_12587. Transport of connexins along the secretory pathway. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P08050. | ||||||||||||||||||
| GermOnline | ENSRNOG00000000805. Rattus norvegicus. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR000500. Connexin. IPR002261. Connexin43. IPR013124. Connexin43_C. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. IPR017991. connexins_rgn. [Graphical view] | ||||||||||||||||||
| PANTHER | PTHR11984. Connexin. 1 hit. | ||||||||||||||||||
| Pfam | PF00029. Connexin. 1 hit. PF03508. Connexin43. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00206. CONNEXIN. PR01132. CONNEXINA1. | ||||||||||||||||||
| SMART | SM00037. CNX. 1 hit. [Graphical view] | ||||||||||||||||||
| PROSITE | PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 603181. | ||||||||||||||||||
Entry information
| Entry name | CXA1_RAT | ||||||||
| Accession | Primary (citable) accession number: P08050 Secondary accession number(s): Q53ZE1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


