Gap junction alpha-1 protein - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P08050 (CXA1_RAT)

Last modified June 16, 2009. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Gap junction alpha-1 protein
Alternative name(s):
    Connexin-43
      Short name=Cx43
    Gap junction 43 kDa heart protein

Gene names

Name:	Gja1
Synonyms:	Cxn-43

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Hide | Top

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Post-translational modification	Contains at least one intramolecular disulfide bond.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Hide | Top

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP transport Inferred from mutant phenotype. Source: RGD apoptosis Inferred from mutant phenotype. Source: RGD cell-cell signaling Inferred from direct assay. Source: RGD gap junction assembly Traceable author statement. Source: UniProtKB heart development Inferred from electronic annotation. Source: InterPro negative regulation of cell proliferation Inferred from direct assay. Source: RGD neuron projection morphogenesis Inferred from mutant phenotype. Source: RGD positive regulation of protein catabolic process Inferred from direct assay. Source: RGD protein oligomerization Inferred from direct assay. Source: RGD regulation of calcium ion transport Inferred from mutant phenotype. Source: RGD response to pH Inferred from direct assay. Source: RGD response to peptide hormone stimulus Inferred from expression pattern. Source: RGD vascular transport Inferred from mutant phenotype. Source: RGD
Cellular component	Golgi-associated vesicle membrane Inferred from Experiment. Source: Reactome connexon complex Inferred from direct assay. Source: RGD early endosome Inferred from direct assay. Source: RGD endoplasmic reticulum membrane Inferred from Experiment. Source: Reactome fascia adherens Inferred from direct assay. Source: RGD integral to plasma membrane Traceable author statement. Source: UniProtKB lysosome Inferred from direct assay. Source: RGD mitochondrial outer membrane Inferred from direct assay. Source: RGD multivesicular body Inferred from direct assay. Source: RGD
Molecular function	PDZ domain binding Inferred from physical interaction. Source: RGD SH3 domain binding Inferred from physical interaction. Source: RGD gap junction channel activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DBN1	Q16643	1	EBI-476947,EBI-351394	From a different organism.
Dbn1	Q9QXS6	1	EBI-476947,EBI-445208	From a different organism.
Sgsm3	Q8VCZ6	1	EBI-476947,EBI-525155	From a different organism.

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.6

Chain

2 – 382

381

Gap junction alpha-1 protein

PRO_0000057804

Regions

Topological domain

2 – 13

Cytoplasmic Probable

Transmembrane

14 – 36

Probable

Topological domain

37 – 76

Extracellular Probable

Transmembrane

77 – 99

Probable

Topological domain

100 – 154

Cytoplasmic Probable

Transmembrane

155 – 177

Probable

Topological domain

178 – 208

Extracellular Probable

Transmembrane

209 – 231

Probable

Topological domain

232 – 382

151

Cytoplasmic Probable

Amino acid modifications

Modified residue

247

Phosphotyrosine By similarity

Modified residue

306

Phosphoserine By similarity

Modified residue

313

Phosphotyrosine By similarity

Modified residue

314

Phosphoserine By similarity

Modified residue

326

Phosphothreonine By similarity

Modified residue

328

Phosphoserine By similarity

Modified residue

330

Phosphoserine By similarity

Modified residue

365

Phosphoserine Ref.9

Modified residue

368

Phosphoserine Ref.9

Modified residue

369

Phosphoserine Ref.9

Modified residue

373

Phosphoserine Ref.9

Disulfide bond

54 ↔ 192

By similarity

Disulfide bond

187 ↔ 198

By similarity

Experimental info

Sequence conflict

G → A AA sequence Ref.5

Sequence conflict

A → T Ref.2

Sequence conflict

V → I AA sequence Ref.5

Secondary structure

382

Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P08050-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 0196416EA6A69490
Show »

FASTA

382

43,031

        10         20         30         40         50         60 
MGDWSALGKL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS AFRCNTQQPG 

        70         80         90        100        110        120 
CENVCYDKSF PISHVRFWVL QIIFVSVPTL LYLAHVFYVM RKEEKLNKKE EELKVAQTDG 

       130        140        150        160        170        180 
VNVEMHLKQI EIKKFKYGIE EHGKVKMRGG LLRTYIISIL FKSVFEVAFL LIQWYIYGFS 

       190        200        210        220        230        240 
LSAVYTCKRD PCPHQVDCFL SRPTEKTIFI IFMLVVSLVS LALNIIELFY VFFKGVKDRV 

       250        260        270        280        290        300 
KGRSDPYHAT TGPLSPSKDC GSPKYAYFNG CSSPTAPLSP MSPPGYKLVT GDRNNSSCRN 

       310        320        330        340        350        360 
YNKQASEQNW ANYSAEQNRM GQAGSTISNS HAQPFDFPDD NQNAKKVAAG HELQPLAIVD 

       370        380 
QRPSSRASSR ASSRPRPDDL EI

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Connexin43: a protein from rat heart homologous to a gap junction protein from liver." Beyer E.C., Paul D.L., Goodenough D.A. J. Cell Biol. 105:2621-2629(1987) [PubMed: 2826492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of a rat uterine gap junction protein and analysis of gene expression during gestation." Lang L.M., Beyer E.C., Schwartz A.L., Gitlin J.D. Am. J. Physiol. 260:E787-E793(1991) [PubMed: 1852114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Uterus.
[3]	Ma L.X., Peng Y.W. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[5]	"The Mr 28,000 gap junction proteins from rat heart and liver are different but related." Nicholson B.J., Gros D.B., Kent S.B.H., Hood L.E., Revel J.-P. J. Biol. Chem. 260:6514-6517(1985) [PubMed: 2987225] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-33. Tissue: Heart.
[6]	"Affinity purification of a rat-brain junctional protein, connexin 43." Dupont E., el Aoumari A., Fromaget C., Briand J.-C., Gros D. Eur. J. Biochem. 200:263-270(1991) [PubMed: 1652440] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE OF 2-16. Tissue: Brain.
[7]	"Connexon integrity is maintained by non-covalent bonds: intramolecular disulfide bonds link the extracellular domains in rat connexin-43." John S.A., Revel J.-P. Biochem. Biophys. Res. Commun. 178:1312-1318(1991) [PubMed: 1651718] [Abstract] Cited for: DISULFIDE BONDS.
[8]	"Membrane topology and quaternary structure of cardiac gap junction ion channels." Yeager M., Gilula N.B. J. Mol. Biol. 223:929-948(1992) [PubMed: 1371548] [Abstract] Cited for: TOPOLOGY.
[9]	"Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells." Yogo K., Ogawa T., Akiyama M., Ishida N., Takeya T. FEBS Lett. 531:132-136(2002) [PubMed: 12417300] [Abstract] Cited for: PHOSPHORYLATION AT SER-365; SER-368; SER-369 AND SER-373.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

X06656 mRNA. Translation: CAA29855.1.
AY324140 mRNA. Translation: AAP88589.1.
BC081842 mRNA. Translation: AAH81842.1.

IPI

IPI00231746.

PIR

A24047.
S00532.

RefSeq

NP_036699.1.

UniGene

Rn.10346

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R5S	NMR	-	A	255-381	[»]
3CYY	X-ray	2.40	C/D	374-382	[»]

DisProt

DP00278.

ModBase

Search...

Protein-protein interaction databases

IntAct

P08050. 4 interactions.

Protein family/group databases

TCDB

1.A.24.1.1. gap junction-forming connexin family.

PTM databases

PhosphoSite

P08050.

Genome annotation databases

Ensembl

ENSRNOG00000000805. Rattus norvegicus. [Contig view]

GeneID

24392.

KEGG

rno:24392.

Organism-specific databases

RGD

2690. Gja1.

Phylogenomic databases

HOVERGEN

P08050.

OMA

P08050. TDGANVD.

Enzyme and pathway databases

Reactome

REACT_12587. Transport of connexins along the secretory pathway.

Gene expression databases

ArrayExpress

P08050.

GermOnline

ENSRNOG00000000805. Rattus norvegicus.

Family and domain databases

InterPro

IPR000500. Connexin.
IPR002261. Connexin43.
IPR013124. Connexin43_C.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
IPR017991. connexins_rgn.
[Graphical view]

PANTHER

PTHR11984. Connexin. 1 hit.

Pfam

PF00029. Connexin. 1 hit.
PF03508. Connexin43. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]

PRINTS

PR00206. CONNEXIN.
PR01132. CONNEXINA1.

SMART

SM00037. CNX. 1 hit.
[Graphical view]

PROSITE

PS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

603181.

Hide | Top

Entry information

Entry name

CXA1_RAT

Accession

Primary (citable) accession number: P08050
Secondary accession number(s): Q53ZE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Hide | Top

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents