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P08048 (ZFY_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger Y-chromosomal protein
Gene names
Name:ZFY
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length801 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transcriptional activator. Binds to the consensus sequence 5'-AGGCCY-3'. Ref.10

Subcellular location

Nucleus.

Domain

The binding of ZFY to DNA is mediated by the interaction of the GGCC core base pairs with zinc fingers 12 and 13. Ref.10

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family. ZFX/ZFY subfamily.

Contains 13 C2H2-type zinc fingers.

Caution

Was originally thought to be the testis determining factor (TDF).

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P08048-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P08048-2)

The sequence of this isoform differs from the canonical sequence as follows:
     21-21: G → V
     22-212: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: P08048-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.
     310-310: N → S
     311-361: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 801801Zinc finger Y-chromosomal protein
PRO_0000047261

Regions

Zinc finger421 – 44323C2H2-type 1
Zinc finger452 – 47423C2H2-type 2; atypical
Zinc finger484 – 50623C2H2-type 3
Zinc finger515 – 53824C2H2-type 4
Zinc finger544 – 56623C2H2-type 5
Zinc finger572 – 59524C2H2-type 6
Zinc finger601 – 62323C2H2-type 7
Zinc finger629 – 65224C2H2-type 8
Zinc finger658 – 68023C2H2-type 9
Zinc finger686 – 70924C2H2-type 10
Zinc finger715 – 73723C2H2-type 11
Zinc finger743 – 76624C2H2-type 12
Zinc finger772 – 79524C2H2-type 13

Amino acid modifications

Modified residue2701Phosphoserine By similarity

Natural variations

Alternative sequence1 – 2626Missing in isoform 3.
VSP_042824
Alternative sequence211G → V in isoform 2.
VSP_042774
Alternative sequence22 – 212191Missing in isoform 2.
VSP_042775
Alternative sequence3101N → S in isoform 3.
VSP_042825
Alternative sequence311 – 36151Missing in isoform 3.
VSP_042826

Experimental info

Sequence conflict3331A → R in AAA72344. Ref.1
Sequence conflict398 – 40710RRPDSRQYQT → ALILLSFPFL Ref.9
Sequence conflict5541R → S Ref.8
Sequence conflict5551H → Y in AAA61310. Ref.2
Sequence conflict6301Missing Ref.8
Sequence conflict7671T → R Ref.8

Secondary structure

....... 801
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2004. Version 3.
Checksum: 956B9B3DA16648FA

FASTA80190,505
        10         20         30         40         50         60 
MDEDEFELQP QEPNSFFDGI GADATHMDGD QIVVEIQEAV FVSNIVDSDI TVHNFVPDDP 

        70         80         90        100        110        120 
DSVVIQDVVE DVVIEEDVQC SDILEEADVS ENVIIPEQVL DSDVTEEVSL PHCTVPDDVL 

       130        140        150        160        170        180 
ASDITSTSMS MPEHVLTSES MHVCDIGHVE HMVHDSVVEA EIITDPLTSD IVSEEVLVAD 

       190        200        210        220        230        240 
CAPEAVIDAS GISVDQQDND KASCEDYLMI SLDDAGKIEH DGSTGVTIDA ESEMDPCKVD 

       250        260        270        280        290        300 
STCPEVIKVY IFKADPGEDD LGGTVDIVES EPENDHGVEL LDQNSSIRVP REKMVYMTVN 

       310        320        330        340        350        360 
DSQQEDEDLN VAEIADEVYM EVIVGEEDAA VAAAAAAVHE QQIDEDEMKT FVPIAWAAAY 

       370        380        390        400        410        420 
GNNSDGIENR NGTASALLHI DESAGLGRLA KQKPKKKRRP DSRQYQTAII IGPDGHPLTV 

       430        440        450        460        470        480 
YPCMICGKKF KSRGFLKRHM KNHPEHLAKK KYHCTDCDYT TNKKISLHNH LESHKLTSKA 

       490        500        510        520        530        540 
EKAIECDECG KHFSHAGALF THKMVHKEKG ANKMHKCKFC EYETAEQGLL NRHLLAVHSK 

       550        560        570        580        590        600 
NFPHICVECG KGFRHPSELR KHMRIHTGEK PYQCQYCEYR SADSSNLKTH IKTKHSKEMP 

       610        620        630        640        650        660 
FKCDICLLTF SDTKEVQQHT LVHQESKTHQ CLHCDHKSSN SSDLKRHVIS VHTKDYPHKC 

       670        680        690        700        710        720 
EMCEKGFHRP SELKKHVAVH KGKKMHQCRH CDFKIADPFV LSRHILSVHT KDLPFRCKRC 

       730        740        750        760        770        780 
RKGFRQQNEL KKHMKTHSGR KVYQCEYCEY STTDASGFKR HVISIHTKDY PHRCEYCKKG 

       790        800 
FRRPSEKNQH IMRHHKEVGL P 

« Hide

Isoform 2 [UniParc].

Checksum: FA9C3797807D5C3C
Show »

FASTA61069,907
Isoform 3 [UniParc].

Checksum: 95DDCD4D9E11A43C
Show »

FASTA72482,165

References

« Hide 'large scale' references
[1]"The putative testis-determining factor and related genes are expressed as discrete-sized transcripts in adult gonadal and somatic tissues."
Lau Y.-F.C., Chan K.
Am. J. Hum. Genet. 45:942-952(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"Comparison of human ZFY and ZFX transcripts."
Palmer M.S., Berta P., Sinclair A.H., Pym B., Goodfellow P.N.
Proc. Natl. Acad. Sci. U.S.A. 87:1681-1685(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Sex chromosomal transposable element accumulation and male-driven substitutional evolution in humans."
Erlandsson R., Wilson J.F., Paabo S.
Mol. Biol. Evol. 17:804-812(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Spleen.
[5]"The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes."
Skaletsky H., Kuroda-Kawaguchi T., Minx P.J., Cordum H.S., Hillier L.W., Brown L.G., Repping S., Pyntikova T., Ali J., Bieri T., Chinwalla A., Delehaunty A., Delehaunty K., Du H., Fewell G., Fulton L., Fulton R., Graves T.A. expand/collapse author list , Hou S.-F., Latrielle P., Leonard S., Mardis E., Maupin R., McPherson J., Miner T., Nash W., Nguyen C., Ozersky P., Pepin K., Rock S., Rohlfing T., Scott K., Schultz B., Strong C., Tin-Wollam A., Yang S.-P., Waterston R.H., Wilson R.K., Rozen S., Page D.C.
Nature 423:825-837(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
[7]"The sex-determining region of the human Y chromosome encodes a finger protein."
Page D.C., Mosher R., Simpson E.M., Fisher E.M.C., Mardon G., Pollack J., McGillivray B., de la Chapelle A., Brown L.G.
Cell 51:1091-1104(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 408-801.
[8]"Comparison of ZFY and ZFX gene structure and analysis of alternative 3' untranslated regions of ZFY."
North M., Sargent C., O'Brien J., Taylor K., Wolfe J., Affara N.A., Ferguson-Smith M.A.
Nucleic Acids Res. 19:2579-2586(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 423-801.
[9]"Evidence for distinguishable transcripts of the putative testis determining gene (ZFY) and mapping of homologous cDNA sequences to chromosomes X,Y and 9."
Affara N.A., Chambers D., O'Brien J., Habeebu S.S.M., Kalaitsidaki M., Bishop C.E., Ferguson-Smith M.A.
Nucleic Acids Res. 17:2987-2999(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 397-446.
[10]"Characterization of the DNA binding activity of the ZFY zinc finger domain."
Grants J., Flanagan E., Yee A., Romaniuk P.J.
Biochemistry 49:679-686(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN DNA-BINDING.
[11]"Alternating zinc fingers in the human male associated protein ZFY: 2D NMR structure of an even finger and implications for 'jumping-linker' DNA recognition."
Kochoyan M., Havel T.F., Nguyen D.Z., Dahl C.E., Keutmann H.T., Weiss M.A.
Biochemistry 30:3371-3386(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF ZINC-FINGERS.
[12]"Alternating zinc fingers in the human male associated protein ZFY: refinement of the NMR structure of an even finger by selective deuterium labeling and implications for DNA recognition."
Kochoyan M., Keutmann H.T., Weiss M.A.
Biochemistry 30:7063-7072(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF ZINC-FINGERS.
[13]"Solvation and the hidden thermodynamics of a zinc finger probed by nonstandard repair of a protein crevice."
Lachenmann M.J., Ladbury J.E., Qian X., Huang K., Singh R., Weiss M.A.
Protein Sci. 13:3115-3126(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 572-598.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10393 mRNA. Translation: AAA72344.1.
M30607 mRNA. Translation: AAA61310.1.
AF114156 Genomic DNA. Translation: AAF21973.1.
AK301061 mRNA. Translation: BAG62669.1.
AC006157 Genomic DNA. Translation: AAD15612.1.
BC114526 mRNA. Translation: AAI14527.1.
BC114960 mRNA. Translation: AAI14961.1.
X15470 Genomic DNA. Translation: CAA33498.1.
CCDSCCDS14774.1. [P08048-1]
CCDS48200.1. [P08048-2]
CCDS48201.1. [P08048-3]
PIRA33630.
RefSeqNP_001138747.1. NM_001145275.1. [P08048-3]
NP_001138748.1. NM_001145276.1. [P08048-2]
NP_003402.2. NM_003411.3. [P08048-1]
XP_005262627.1. XM_005262570.1. [P08048-1]
XP_006724936.1. XM_006724873.1. [P08048-1]
UniGeneHs.522845.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KLRNMR-A570-598[»]
1KLSNMR-A570-598[»]
1XRZNMR-A570-598[»]
5ZNFNMR-A570-598[»]
7ZNFNMR-A570-598[»]
ProteinModelPortalP08048.
SMRP08048. Positions 421-794.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113376. 3 interactions.
STRING9606.ENSP00000155093.

PTM databases

PhosphoSiteP08048.

Polymorphism databases

DMDM51338719.

Proteomic databases

MaxQBP08048.
PaxDbP08048.
PRIDEP08048.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000155093; ENSP00000155093; ENSG00000067646. [P08048-1]
ENST00000383052; ENSP00000372525; ENSG00000067646. [P08048-1]
ENST00000431102; ENSP00000402502; ENSG00000067646. [P08048-2]
ENST00000449237; ENSP00000393908; ENSG00000067646. [P08048-3]
GeneID7544.
KEGGhsa:7544.
UCSCuc004fqj.3. human. [P08048-1]
uc010nwe.3. human. [P08048-3]
uc011nan.2. human. [P08048-2]

Organism-specific databases

CTD7544.
GeneCardsGC0YP002846.
HGNCHGNC:12870. ZFY.
HPAHPA001748.
MIM490000. gene.
neXtProtNX_P08048.
PharmGKBPA37459.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000231717.
HOVERGENHBG000337.
InParanoidP08048.
OMATCVMALE.
OrthoDBEOG7P8P73.
PhylomeDBP08048.
TreeFamTF335557.

Gene expression databases

ArrayExpressP08048.
BgeeP08048.
CleanExHS_ZFY.
GenevestigatorP08048.

Family and domain databases

Gene3D3.30.160.60. 7 hits.
InterProIPR006794. Transcrp_activ_Zfx/Zfy-dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 4 hits.
PF04704. Zfx_Zfy_act. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 13 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP08048.
GeneWikiZFY.
GenomeRNAi7544.
NextBio29517.
PROP08048.
SOURCESearch...

Entry information

Entry nameZFY_HUMAN
AccessionPrimary (citable) accession number: P08048
Secondary accession number(s): B4DVF7 expand/collapse secondary AC list , Q14021, Q15558, Q1RME9, Q24JR0, Q96TF3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome Y

Human chromosome Y: entries, gene names and cross-references to MIM