Reviewed, UniProtKB/Swiss-Prot P08048 (ZFY_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger Y-chromosomal protein

Gene names

Name:

ZFY

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcriptional activator.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. ZFX/ZFY subfamily. Contains 13 C2H2-type zinc fingers.
Caution	Was originally thought to be the testis determining factor (TDF).

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription regulator activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 801

801

Zinc finger Y-chromosomal protein

PRO_0000047261

Regions

Zinc finger

421 – 443

23

C2H2-type 1

Zinc finger

452 – 474

23

C2H2-type 2; atypical

Zinc finger

484 – 506

23

C2H2-type 3

Zinc finger

515 – 538

24

C2H2-type 4

Zinc finger

544 – 566

23

C2H2-type 5

Zinc finger

572 – 595

24

C2H2-type 6

Zinc finger

601 – 623

23

C2H2-type 7

Zinc finger

629 – 652

24

C2H2-type 8

Zinc finger

658 – 680

23

C2H2-type 9

Zinc finger

686 – 709

24

C2H2-type 10

Zinc finger

715 – 737

23

C2H2-type 11

Zinc finger

743 – 766

24

C2H2-type 12

Zinc finger

772 – 795

24

C2H2-type 13

Experimental info

Sequence conflict

333

1

A → R in AAA72344. Ref.1

Sequence conflict

398 – 407

10

RRPDSRQYQT → ALILLSFPFL Ref.8

Sequence conflict

554

1

R → S Ref.7

Sequence conflict

555

1

H → Y in AAA61310. Ref.2

Sequence conflict

630

1

Missing Ref.7

Sequence conflict

767

1

T → R Ref.7

Secondary structure

1

.

.

.

.

.

801

Helix Strand Turn

Details...

Beta strand

575 – 578

4

Helix

585 – 594

10

Sequences

	Sequence		Length	Mass (Da)
	P08048-1 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: 956B9B3DA16648FA	FASTA	801	90,505
	10 20 30 40 50 60 MDEDEFELQP QEPNSFFDGI GADATHMDGD QIVVEIQEAV FVSNIVDSDI TVHNFVPDDP 70 80 90 100 110 120 DSVVIQDVVE DVVIEEDVQC SDILEEADVS ENVIIPEQVL DSDVTEEVSL PHCTVPDDVL 130 140 150 160 170 180 ASDITSTSMS MPEHVLTSES MHVCDIGHVE HMVHDSVVEA EIITDPLTSD IVSEEVLVAD 190 200 210 220 230 240 CAPEAVIDAS GISVDQQDND KASCEDYLMI SLDDAGKIEH DGSTGVTIDA ESEMDPCKVD 250 260 270 280 290 300 STCPEVIKVY IFKADPGEDD LGGTVDIVES EPENDHGVEL LDQNSSIRVP REKMVYMTVN 310 320 330 340 350 360 DSQQEDEDLN VAEIADEVYM EVIVGEEDAA VAAAAAAVHE QQIDEDEMKT FVPIAWAAAY 370 380 390 400 410 420 GNNSDGIENR NGTASALLHI DESAGLGRLA KQKPKKKRRP DSRQYQTAII IGPDGHPLTV 430 440 450 460 470 480 YPCMICGKKF KSRGFLKRHM KNHPEHLAKK KYHCTDCDYT TNKKISLHNH LESHKLTSKA 490 500 510 520 530 540 EKAIECDECG KHFSHAGALF THKMVHKEKG ANKMHKCKFC EYETAEQGLL NRHLLAVHSK 550 560 570 580 590 600 NFPHICVECG KGFRHPSELR KHMRIHTGEK PYQCQYCEYR SADSSNLKTH IKTKHSKEMP 610 620 630 640 650 660 FKCDICLLTF SDTKEVQQHT LVHQESKTHQ CLHCDHKSSN SSDLKRHVIS VHTKDYPHKC 670 680 690 700 710 720 EMCEKGFHRP SELKKHVAVH KGKKMHQCRH CDFKIADPFV LSRHILSVHT KDLPFRCKRC 730 740 750 760 770 780 RKGFRQQNEL KKHMKTHSGR KVYQCEYCEY STTDASGFKR HVISIHTKDY PHRCEYCKKG 790 800 FRRPSEKNQH IMRHHKEVGL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The putative testis-determining factor and related genes are expressed as discrete-sized transcripts in adult gonadal and somatic tissues." Lau Y.-F.C., Chan K. Am. J. Hum. Genet. 45:942-952(1989) [PubMed: 2511751] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Comparison of human ZFY and ZFX transcripts." Palmer M.S., Berta P., Sinclair A.H., Pym B., Goodfellow P.N. Proc. Natl. Acad. Sci. U.S.A. 87:1681-1685(1990) [PubMed: 2308929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sex chromosomal transposable element accumulation and male-driven substitutional evolution in humans." Erlandsson R., Wilson J.F., Paabo S. Mol. Biol. Evol. 17:804-812(2000) [PubMed: 10779541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes." Skaletsky H., Kuroda-Kawaguchi T., Minx P.J., Cordum H.S., Hillier L.W., Brown L.G., Repping S., Pyntikova T., Ali J., Bieri T., Chinwalla A., Delehaunty A., Delehaunty K., Du H., Fewell G., Fulton L., Fulton R., Graves T.A. , Hou S.-F., Latrielle P., Leonard S., Mardis E., Maupin R., McPherson J., Miner T., Nash W., Nguyen C., Ozersky P., Pepin K., Rock S., Rohlfing T., Scott K., Schultz B., Strong C., Tin-Wollam A., Yang S.-P., Waterston R.H., Wilson R.K., Rozen S., Page D.C. Nature 423:825-837(2003) [PubMed: 12815422] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The sex-determining region of the human Y chromosome encodes a finger protein." Page D.C., Mosher R., Simpson E.M., Fisher E.M.C., Mardon G., Pollack J., McGillivray B., de la Chapelle A., Brown L.G. Cell 51:1091-1104(1987) [PubMed: 3690661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 408-801.
[7]	"Comparison of ZFY and ZFX gene structure and analysis of alternative 3' untranslated regions of ZFY." North M., Sargent C., O'Brien J., Taylor K., Wolfe J., Affara N.A., Ferguson-Smith M.A. Nucleic Acids Res. 19:2579-2586(1991) [PubMed: 2041734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 423-801.
[8]	"Evidence for distinguishable transcripts of the putative testis determining gene (ZFY) and mapping of homologous cDNA sequences to chromosomes X,Y and 9." Affara N.A., Chambers D., O'Brien J., Habeebu S.S.M., Kalaitsidaki M., Bishop C.E., Ferguson-Smith M.A. Nucleic Acids Res. 17:2987-2999(1989) [PubMed: 2498838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 397-446.
[9]	"Alternating zinc fingers in the human male associated protein ZFY: 2D NMR structure of an even finger and implications for 'jumping-linker' DNA recognition." Kochoyan M., Havel T.F., Nguyen D.Z., Dahl C.E., Keutmann H.T., Weiss M.A. Biochemistry 30:3371-3386(1991) [PubMed: 1849423] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-FINGERS.
[10]	"Alternating zinc fingers in the human male associated protein ZFY: refinement of the NMR structure of an even finger by selective deuterium labeling and implications for DNA recognition." Kochoyan M., Keutmann H.T., Weiss M.A. Biochemistry 30:7063-7072(1991) [PubMed: 1854720] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-FINGERS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L10393 mRNA. Translation: AAA72344.1.
M30607 mRNA. Translation: AAA61310.1.
AF114156 Genomic DNA. Translation: AAF21973.1.
AC006157 Genomic DNA. Translation: AAD15612.1.
BC114960 mRNA. Translation: AAI14961.1.
X15470 Genomic DNA. Translation: CAA33498.1.

IPI

IPI00027202.

PIR

A33630.

RefSeq

NP_001138747.1.
NP_003402.2.

UniGene

Hs.522845

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KLR	NMR	-	A	572-598	[»]
1KLS	NMR	-	A	572-598	[»]
1XRZ	NMR	-	A	572-598	[»]
5ZNF	NMR	-	A	572-598	[»]
7ZNF	NMR	-	A	572-598	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P08048.

PTM databases

PhosphoSite

P08048.

Proteomic databases

PRIDE

P08048.

Genome annotation databases

Ensembl

ENST00000155093; ENSP00000155093; ENSG00000067646; Homo sapiens. [Genome view]
ENST00000383052; ENSP00000372525; ENSG00000067646; Homo sapiens. [Genome view]

GeneID

7544.

KEGG

hsa:7544.

UCSC

uc004fqj.1. human.

Organism-specific databases

CTD

7544.

GeneCards

GC0YP002846.
GC0YP002863.

H-InvDB

HIX0017180.

HGNC

HGNC:12870. ZFY.

HPA

HPA001748.

MIM

490000. gene.

PharmGKB

PA37459.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P08048.

HOVERGEN

P08048.

OMA

QIDEDEM

Gene expression databases

ArrayExpress

P08048.

Bgee

P08048.

CleanEx

HS_ZFY.

Genevestigator

P08048.

GermOnline

ENSG00000067646. Homo sapiens.

Family and domain databases

InterPro

IPR006794. Zfx/Zfy_activation_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.

Pfam

PF00096. zf-C2H2. 12 hits.
PF04704. Zfx_Zfy_act. 1 hit.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 13 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 12 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

ZFY_HUMAN

Accession

Primary (citable) accession number: P08048
Secondary accession number(s): Q14021 , Q15558, Q1RME9, Q96TF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 16, 2004
Last modified:	November 24, 2009
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.