Reviewed,
UniProtKB/Swiss-Prot P08047 (SP1_HUMAN)
Last modified
July 7, 2009.
Version 122.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Transcription factor Sp1 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 785 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter. |
| Subunit structure | Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding. Ref.8 Ref.11 Ref.13 Ref.14 Ref.16 |
| Subcellular location | |
| Post-translational modification | O-glycosylated; contains N-acetylglucosamine side chains. Ref.7 |
| Sequence similarities | Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-298336,EBI-298336 | ||
| ABL1 | P00519 | 1 | EBI-298336,EBI-375543 | |
| ATF7IP | Q6VMQ6 | 1 | EBI-298336,EBI-928732 | |
| CRK | P46108 | 1 | EBI-298336,EBI-886 | |
| E2F1 | Q01094 | 2 | EBI-298336,EBI-448924 | |
| ELF1 | P32519 | 1 | EBI-298336,EBI-765526 | |
| FYN | P06241 | 1 | EBI-298336,EBI-515315 | |
| GRB2 | P62993 | 1 | EBI-298336,EBI-401755 | |
| HCFC1 | P51610 | 2 | EBI-298336,EBI-396176 | |
| KLF10 | Q13118 | 1 | EBI-298336,EBI-1389509 | |
| MYC | P01106 | 2 | EBI-298336,EBI-447544 | |
| NCK1 | P16333 | 1 | EBI-298336,EBI-389883 | |
| Nfya | P18576 | 1 | EBI-298336,EBI-862695 | From a different organism. |
| Nfya | P23708 | 2 | EBI-298336,EBI-862337 | From a different organism. |
| PHC2 | Q8IXK0 | 1 | EBI-298336,EBI-713786 | |
| POGZ | Q7Z3K3 | 1 | EBI-298336,EBI-1389308 | |
| POU2F1 | P14859 | 1 | EBI-298336,EBI-624770 | |
| RNASEN | Q9NRR4 | 1 | EBI-298336,EBI-528367 | |
| RNF19A | Q9NV58 | 1 | EBI-298336,EBI-1390270 | |
| SF3A1 | Q15459 | 1 | EBI-298336,EBI-1054743 | |
| SRC | P12931 | 1 | EBI-298336,EBI-621482 | |
| SREBF2 | Q12772 | 1 | EBI-298336,EBI-465059 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||
Molecule processing | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 785 | 785 | Transcription factor Sp1 | PRO_0000047137 | |||||||||||||||||||
Regions | |||||||||||||||||||||||
| Zinc finger | 626 – 650 | 25 | C2H2-type 1 | ||||||||||||||||||||
| Zinc finger | 656 – 680 | 25 | C2H2-type 2 | ||||||||||||||||||||
| Zinc finger | 686 – 708 | 23 | C2H2-type 3 | ||||||||||||||||||||
| Region | 619 – 785 | 167 | VZV IE62-binding | ||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||
| Modified residue | 7 | 1 | Phosphoserine By similarity | ||||||||||||||||||||
| Modified residue | 59 | 1 | Phosphoserine Ref.15 Ref.17 | ||||||||||||||||||||
| Modified residue | 651 | 1 | Phosphothreonine Ref.18 | ||||||||||||||||||||
Natural variations | |||||||||||||||||||||||
| Natural variant | 737 | 1 | T → A: dbSNP rs3741665. | VAR_019971 | |||||||||||||||||||
Experimental info | |||||||||||||||||||||||
| Sequence conflict | 366 | 1 | D → G AA sequence Ref.4 | ||||||||||||||||||||
| Sequence conflict | 670 | 1 | S → F AA sequence Ref.4 | ||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||
| Beta strand | 620 – 622 | 3 | |||||||||||||||||||||
| Beta strand | 636 – 638 | 3 | |||||||||||||||||||||
| Helix | 640 – 651 | 12 | |||||||||||||||||||||
| Helix | 670 – 677 | 8 | |||||||||||||||||||||
| Turn | 678 – 680 | 3 | |||||||||||||||||||||
| Turn | 689 – 692 | 4 | |||||||||||||||||||||
| Helix | 699 – 706 | 8 | |||||||||||||||||||||
| Helix | 707 – 709 | 3 | |||||||||||||||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis. |
| [2] | Haggart M.H., Ladurner A.G. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-785. Tissue: Cervix carcinoma. |
| [3] | "Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing." Takahara T., Kanazu S., Yanagisawa S., Akanuma H. J. Biol. Chem. 275:38067-38072(2000) [PubMed: 10973950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-558. |
| [4] | "Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain." Kadonaga J.T., Carner K.R., Masiarz F.R., Tjian R. Cell 51:1079-1090(1987) [PubMed: 3319186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 90-785, PROTEIN SEQUENCE OF 359-375 AND 670-675. |
| [5] | "Expression of transcription factor Sp1 mRNA in mammalian cells." Nicolas M., Noe V., Ciudad C.J. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-109. |
| [6] | "Sequencing of the 5' end of human transcription factor SP1 mRNA." Handschug K., Huebner A. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-98. |
| [7] | "O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation." Jackson S.P., Tjian R. Cell 55:125-133(1988) [PubMed: 3139301] [Abstract] Cited for: GLYCOSYLATION. |
| [8] | "Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and trans-activation of viral long terminal repeat." Wang L., Mukherjee S., Jia F., Narayan O., Zhao L.J. J. Biol. Chem. 270:25564-25569(1995) [PubMed: 7592727] [Abstract] Cited for: INTERACTION WITH HIV-1 VPR. |
| [9] | "The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1." Parks C.L., Shenk T. J. Biol. Chem. 271:4417-4430(1996) [PubMed: 8626793] [Abstract] Cited for: IDENTIFICATION OF SEROTONIN 1A RECEPTOR PROMOTER BINDING SITES. |
| [10] | "The SV40 capsid protein VP3 cooperates with the cellular transcription factor Sp1 in DNA-binding and in regulating viral promoter activity." Gordon-Shaag A., Ben-Nun-Shaul O., Kasamatsu H., Oppenheim A.B., Oppenheim A. J. Mol. Biol. 275:187-195(1998) [PubMed: 9466902] [Abstract] Cited for: INTERACTION WITH SV40 VP2/3. |
| [11] | "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening." Gunther M., Laithier M., Brison O. Mol. Cell. Biochem. 210:131-142(2000) [PubMed: 10976766] [Abstract] Cited for: INTERACTION WITH ATF7IP; PHC2; POGZ AND HCFC1. Tissue: Colon. |
| [12] | "Cellular transcription factor Sp1 recruits simian virus 40 capsid proteins to the viral packaging signal, ses." Gordon-Shaag A., Ben-Nun-Shaul O., Roitman V., Yosef Y., Oppenheim A. J. Virol. 76:5915-5924(2002) [PubMed: 12021324] [Abstract] Cited for: INTERACTION WITH SV40 VP1. |
| [13] | "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter." Di Padova M., Bruno T., De Nicola F., Iezzi S., D'Angelo C., Gallo R., Nicosia D., Corbi N., Biroccio A., Floridi A., Passananti C., Fanciulli M. J. Biol. Chem. 278:36496-36504(2003) [PubMed: 12847090] [Abstract] Cited for: INTERACTION WITH AATF. |
| [14] | "Interaction between the varicella zoster virus IE62 major transactivator and cellular transcription factor Sp1." Peng H., He H., Hay J., Ruyechan W.T. J. Biol. Chem. 278:38068-38075(2003) [PubMed: 12855699] [Abstract] Cited for: INTERACTION WITH VARICELLA-ZOSTER VIRUS IE62 PROTEIN. |
| [15] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, MASS SPECTROMETRY. Tissue: Epithelium. |
| [16] | "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins." Ichimura T., Watanabe S., Sakamoto Y., Aoto T., Fujita N., Nakao M. J. Biol. Chem. 280:13928-13935(2005) [PubMed: 15691849] [Abstract] Cited for: INTERACTION WITH ATF7IP AND ATF7IP2. |
| [17] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, MASS SPECTROMETRY. Tissue: Epithelium. |
| [18] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-651, MASS SPECTROMETRY. |
| [19] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [20] | "Structures of zinc finger domains from transcription factor Sp1. Insights into sequence-specific protein-DNA recognition." Narayan V.A., Kriwacki R.W., Caradonna J.P. J. Biol. Chem. 272:7801-7809(1997) [PubMed: 9065444] [Abstract] Cited for: STRUCTURE BY NMR OF 654-684 AND 684-712. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BC043224 mRNA. Translation: AAH43224.1. Different initiation. BC062539 mRNA. Translation: AAH62539.1. AF252284 mRNA. Translation: AAF67726.1. AB039286 Genomic DNA. Translation: BAB13476.1. J03133 mRNA. Translation: AAA61154.1. AF255682 mRNA. Translation: AAF78781.1. AJ272134 mRNA. Translation: CAB75345.1. | |||||||||||||||||||||||||||||||||||||
| IPI | IPI00465152. | ||||||||||||||||||||||||||||||||||||
| PIR | A29635. | ||||||||||||||||||||||||||||||||||||
| RefSeq | NP_003100.1. NP_612482.2. | ||||||||||||||||||||||||||||||||||||
| UniGene | Hs.620754 Hs.649191 | ||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||
| DisProt | DP00378. | ||||||||||||||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||||||||
| DIP | DIP:36N. | ||||||||||||||||||||||||||||||||||||
| IntAct | P08047. 28 interactions. | ||||||||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||||||||
| GlycoSuiteDB | P08047. | ||||||||||||||||||||||||||||||||||||
| PhosphoSite | P08047. | ||||||||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||||||||
| PeptideAtlas | P08047. | ||||||||||||||||||||||||||||||||||||
| PRIDE | P08047. | ||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||
| Ensembl | ENSG00000185591. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||||||||
| GeneID | 6667. | ||||||||||||||||||||||||||||||||||||
| KEGG | hsa:6667. | ||||||||||||||||||||||||||||||||||||
| UCSC | uc001scw.1. human. | ||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||
| GeneCards | GC12P052060. | ||||||||||||||||||||||||||||||||||||
| H-InvDB | HIX0010670. | ||||||||||||||||||||||||||||||||||||
| HGNC | HGNC:11205. SP1. | ||||||||||||||||||||||||||||||||||||
| HPA | CAB000330. HPA001853. HPA012292. | ||||||||||||||||||||||||||||||||||||
| MIM | 189906. gene. | ||||||||||||||||||||||||||||||||||||
| PharmGKB | PA25061. | ||||||||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||
| HOGENOM | P08047. | ||||||||||||||||||||||||||||||||||||
| HOVERGEN | P08047. | ||||||||||||||||||||||||||||||||||||
| OMA | P08047. TITLAPM. | ||||||||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||||||||
| Pathway_Interaction_DB | hnf3apathway. FOXA1 transcription factor network. hnf3bpathway. FOXA2 and FOXA3 transcription factor networks. foxm1pathway. FOXM1 transcription factor network. hif1_tfpathway. HIF-1-alpha transcription factor network. il2_stat5pathway. IL2 signaling events mediated by STAT5. il4_2pathway. IL4-mediated signaling events. smad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling. telomerasepathway. Regulation of Telomerase. | ||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||
| ArrayExpress | P08047. | ||||||||||||||||||||||||||||||||||||
| Bgee | P08047. | ||||||||||||||||||||||||||||||||||||
| CleanEx | HS_SP1. | ||||||||||||||||||||||||||||||||||||
| GermOnline | ENSG00000185591. Homo sapiens. | ||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||
| InterPro | IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| Gene3D | G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits. | ||||||||||||||||||||||||||||||||||||
| Pfam | PF00096. zf-C2H2. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| ProDom | PD000003. Znf_C2H2. 2 hits. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||||||||||||||
| SMART | SM00355. ZnF_C2H2. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| PROSITE | PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||
| NextBio | 25995. | ||||||||||||||||||||||||||||||||||||
| PMAP-CutDB | P08047. | ||||||||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | SP1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P08047 Secondary accession number(s): Q86TN8 Q9NYE7 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 12 Human chromosome 12: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


