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Protein

Early growth response protein 1

Gene

Egr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri336 – 36025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri366 – 38823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri394 – 41623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • BMP signaling pathway Source: MGI
  • cellular response to gamma radiation Source: BHF-UCL
  • cellular response to heparin Source: UniProtKB
  • cellular response to mycophenolic acid Source: UniProtKB
  • cellular response to organic substance Source: MGI
  • glomerular mesangial cell proliferation Source: UniProtKB
  • interleukin-1-mediated signaling pathway Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of glomerular metanephric mesangial cell proliferation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • regulation of apoptotic process Source: MGI
  • regulation of protein sumoylation Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: MGI
  • response to glucose Source: UniProtKB
  • response to insulin Source: UniProtKB
  • skeletal muscle cell differentiation Source: MGI
  • T cell differentiation Source: MGI
  • transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 1
Short name:
EGR-1
Alternative name(s):
Nerve growth factor-induced protein A
Short name:
NGFI-A
Transcription factor Zif268
Zinc finger protein Krox-24
Gene namesi
Name:Egr1
Synonyms:Egr-1, Krox-24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:95295. Egr1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Early growth response protein 1PRO_0000047110Add
BLAST

Proteomic databases

PaxDbiP08046.
PRIDEiP08046.

PTM databases

iPTMnetiP08046.
PhosphoSiteiP08046.

Expressioni

Inductioni

By growth factors.

Gene expression databases

BgeeiENSMUSG00000038418.
CleanExiMM_EGR1.
ExpressionAtlasiP08046. baseline and differential.
GenevisibleiP08046. MM.

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199404. 7 interactions.
STRINGi10090.ENSMUSP00000069616.

Structurei

Secondary structure

1
533
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi335 – 3373Combined sources
Beta strandi340 – 3423Combined sources
Beta strandi346 – 3494Combined sources
Helixi350 – 36112Combined sources
Turni369 – 3713Combined sources
Beta strandi374 – 3763Combined sources
Helixi378 – 38912Combined sources
Turni397 – 3993Combined sources
Beta strandi402 – 4054Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A1FX-ray2.10A333-421[»]
1A1GX-ray1.90A333-421[»]
1A1HX-ray1.60A333-421[»]
1A1IX-ray1.60A333-421[»]
1A1JX-ray2.00A333-421[»]
1A1KX-ray1.90A333-421[»]
1A1LX-ray2.30A333-421[»]
1AAYX-ray1.60A333-421[»]
1F2IX-ray2.35G/H/I/J/K/L331-389[»]
1G2DX-ray2.20C/F333-421[»]
1G2FX-ray2.00C/F333-421[»]
1JK1X-ray1.90A333-421[»]
1JK2X-ray1.65A333-421[»]
1LLMX-ray1.50C/D364-412[»]
1P47X-ray2.20A/B333-419[»]
1ZAAX-ray2.10C332-418[»]
ProteinModelPortaliP08046.
SMRiP08046. Positions 333-453.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08046.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi57 – 8125Gly/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri336 – 36025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri366 – 38823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri394 – 41623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08046.
KOiK09203.
OMAiFATQTGS.
OrthoDBiEOG091G06VX.
PhylomeDBiP08046.
TreeFamiTF318980.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 1 hit.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG
60 70 80 90 100
AAGTPEGSGG NSSSSTSSGG GGGGGSNSGS SAFNPQGEPS EQPYEHLTTE
110 120 130 140 150
SFSDIALNNE KAMVETSYPS QTTRLPPITY TGRFSLEPAP NSGNTLWPEP
160 170 180 190 200
LFSLVSGLVS MTNPPTSSSS APSPAASSSS SASQSPPLSC AVPSNDSSPI
210 220 230 240 250
YSAAPTFPTP NTDIFPEPQS QAFPGSAGTA LQYPPPAYPA TKGGFQVPMI
260 270 280 290 300
PDYLFPQQQG DLSLGTPDQK PFQGLENRTQ QPSLTPLSTI KAFATQSGSQ
310 320 330 340 350
DLKALNTTYQ SQLIKPSRMR KYPNRPSKTP PHERPYACPV ESCDRRFSRS
360 370 380 390 400
DELTRHIRIH TGQKPFQCRI CMRNFSRSDH LTTHIRTHTG EKPFACDICG
410 420 430 440 450
RKFARSDERK RHTKIHLRQK DKKADKSVVA SPAASSLSSY PSPVATSYPS
460 470 480 490 500
PATTSFPSPV PTSYSSPGSS TYPSPAHSGF PSPSVATTFA SVPPAFPTQV
510 520 530
SSFPSAGVSS SFSTSTGLSD MTATFSPRTI EIC
Length:533
Mass (Da):56,590
Last modified:November 1, 1990 - v2
Checksum:i36E3935767CEAA0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761S → T (PubMed:2511075).Curated
Sequence conflicti76 – 761S → T (PubMed:3133658).Curated
Sequence conflicti80 – 801S → T (PubMed:2511075).Curated
Sequence conflicti80 – 801S → T (PubMed:3133658).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20157 mRNA. Translation: AAA37544.1.
M22326 mRNA. Translation: AAA40416.1.
M28845, M28844 Genomic DNA. Translation: AAB00468.1.
M19643 mRNA. Translation: AAA39382.1.
CCDSiCCDS29136.1.
PIRiJS0304.
RefSeqiNP_031939.1. NM_007913.5.
UniGeneiMm.181959.

Genome annotation databases

EnsembliENSMUST00000064795; ENSMUSP00000069616; ENSMUSG00000038418.
ENSMUST00000165033; ENSMUSP00000126931; ENSMUSG00000038418.
GeneIDi13653.
KEGGimmu:13653.
UCSCiuc008elt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20157 mRNA. Translation: AAA37544.1.
M22326 mRNA. Translation: AAA40416.1.
M28845, M28844 Genomic DNA. Translation: AAB00468.1.
M19643 mRNA. Translation: AAA39382.1.
CCDSiCCDS29136.1.
PIRiJS0304.
RefSeqiNP_031939.1. NM_007913.5.
UniGeneiMm.181959.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A1FX-ray2.10A333-421[»]
1A1GX-ray1.90A333-421[»]
1A1HX-ray1.60A333-421[»]
1A1IX-ray1.60A333-421[»]
1A1JX-ray2.00A333-421[»]
1A1KX-ray1.90A333-421[»]
1A1LX-ray2.30A333-421[»]
1AAYX-ray1.60A333-421[»]
1F2IX-ray2.35G/H/I/J/K/L331-389[»]
1G2DX-ray2.20C/F333-421[»]
1G2FX-ray2.00C/F333-421[»]
1JK1X-ray1.90A333-421[»]
1JK2X-ray1.65A333-421[»]
1LLMX-ray1.50C/D364-412[»]
1P47X-ray2.20A/B333-419[»]
1ZAAX-ray2.10C332-418[»]
ProteinModelPortaliP08046.
SMRiP08046. Positions 333-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199404. 7 interactions.
STRINGi10090.ENSMUSP00000069616.

PTM databases

iPTMnetiP08046.
PhosphoSiteiP08046.

Proteomic databases

PaxDbiP08046.
PRIDEiP08046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064795; ENSMUSP00000069616; ENSMUSG00000038418.
ENSMUST00000165033; ENSMUSP00000126931; ENSMUSG00000038418.
GeneIDi13653.
KEGGimmu:13653.
UCSCiuc008elt.1. mouse.

Organism-specific databases

CTDi1958.
MGIiMGI:95295. Egr1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08046.
KOiK09203.
OMAiFATQTGS.
OrthoDBiEOG091G06VX.
PhylomeDBiP08046.
TreeFamiTF318980.

Miscellaneous databases

ChiTaRSiEgr1. mouse.
EvolutionaryTraceiP08046.
PROiP08046.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038418.
CleanExiMM_EGR1.
ExpressionAtlasiP08046. baseline and differential.
GenevisibleiP08046. MM.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 1 hit.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGR1_MOUSE
AccessioniPrimary (citable) accession number: P08046
Secondary accession number(s): Q61777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1990
Last modified: September 7, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.