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P08046

- EGR1_MOUSE

UniProt

P08046 - EGR1_MOUSE

Protein

Early growth response protein 1

Gene

Egr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri336 – 36025C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri366 – 38823C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri394 – 41623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. double-stranded DNA binding Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    5. RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
    6. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    7. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    8. transcription regulatory region DNA binding Source: BHF-UCL

    GO - Biological processi

    1. BMP signaling pathway Source: MGI
    2. cellular response to antibiotic Source: Ensembl
    3. cellular response to cAMP Source: RefGenome
    4. cellular response to drug Source: Ensembl
    5. cellular response to follicle-stimulating hormone stimulus Source: Ensembl
    6. cellular response to gamma radiation Source: BHF-UCL
    7. cellular response to gonadotropin stimulus Source: RefGenome
    8. cellular response to growth factor stimulus Source: Ensembl
    9. cellular response to heparin Source: UniProtKB
    10. cellular response to hyperoxia Source: Ensembl
    11. cellular response to hypoxia Source: Ensembl
    12. cellular response to insulin stimulus Source: Ensembl
    13. cellular response to isoquinoline alkaloid Source: Ensembl
    14. cellular response to mechanical stimulus Source: Ensembl
    15. cellular response to mycophenolic acid Source: UniProtKB
    16. cellular response to organic substance Source: MGI
    17. cellular response to steroid hormone stimulus Source: Ensembl
    18. circadian rhythm Source: Ensembl
    19. glomerular mesangial cell proliferation Source: UniProtKB
    20. interleukin-1-mediated signaling pathway Source: Ensembl
    21. learning or memory Source: Ensembl
    22. long-term synaptic potentiation Source: Ensembl
    23. negative regulation of apoptotic process Source: RefGenome
    24. negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
    25. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    26. oligodendrocyte differentiation Source: Ensembl
    27. positive regulation of glomerular metanephric mesangial cell proliferation Source: UniProtKB
    28. positive regulation of neuron apoptotic process Source: Ensembl
    29. positive regulation of transcription, DNA-templated Source: UniProtKB
    30. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    31. regulation of long-term neuronal synaptic plasticity Source: RefGenome
    32. regulation of protein sumoylation Source: Ensembl
    33. regulation of transcription, DNA-templated Source: MGI
    34. response to amphetamine Source: Ensembl
    35. response to carbon monoxide Source: Ensembl
    36. response to cocaine Source: Ensembl
    37. response to electrical stimulus Source: Ensembl
    38. response to ethanol Source: Ensembl
    39. response to glucose Source: UniProtKB
    40. response to insulin Source: UniProtKB
    41. response to norepinephrine Source: Ensembl
    42. response to nutrient levels Source: Ensembl
    43. skeletal muscle cell differentiation Source: MGI
    44. T cell differentiation Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Early growth response protein 1
    Short name:
    EGR-1
    Alternative name(s):
    Nerve growth factor-induced protein A
    Short name:
    NGFI-A
    Transcription factor Zif268
    Zinc finger protein Krox-24
    Gene namesi
    Name:Egr1
    Synonyms:Egr-1, Krox-24
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:95295. Egr1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Early growth response protein 1PRO_0000047110Add
    BLAST

    Proteomic databases

    PRIDEiP08046.

    PTM databases

    PhosphoSiteiP08046.

    Expressioni

    Inductioni

    By growth factors.

    Gene expression databases

    ArrayExpressiP08046.
    BgeeiP08046.
    CleanExiMM_EGR1.
    GenevestigatoriP08046.

    Interactioni

    Subunit structurei

    Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.By similarity

    Protein-protein interaction databases

    BioGridi199404. 7 interactions.

    Structurei

    Secondary structure

    1
    533
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi335 – 3373
    Beta strandi340 – 3423
    Beta strandi346 – 3494
    Helixi350 – 36112
    Turni369 – 3713
    Beta strandi374 – 3763
    Helixi378 – 38912
    Turni397 – 3993
    Beta strandi402 – 4054
    Helixi406 – 4127
    Helixi414 – 4174

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A1FX-ray2.10A333-421[»]
    1A1GX-ray1.90A333-421[»]
    1A1HX-ray1.60A333-421[»]
    1A1IX-ray1.60A333-421[»]
    1A1JX-ray2.00A333-421[»]
    1A1KX-ray1.90A333-421[»]
    1A1LX-ray2.30A333-421[»]
    1AAYX-ray1.60A333-421[»]
    1F2IX-ray2.35G/H/I/J/K/L331-389[»]
    1G2DX-ray2.20C/F333-421[»]
    1G2FX-ray2.00C/F333-421[»]
    1JK1X-ray1.90A333-421[»]
    1JK2X-ray1.65A333-421[»]
    1LLMX-ray1.50C/D364-412[»]
    1P47X-ray2.20A/B333-419[»]
    1ZAAX-ray2.10C332-418[»]
    ProteinModelPortaliP08046.
    SMRiP08046. Positions 334-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08046.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi57 – 8125Gly/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri336 – 36025C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri366 – 38823C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri394 – 41623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000036856.
    HOVERGENiHBG003909.
    InParanoidiP08046.
    KOiK09203.
    OMAiAVTNSFS.
    OrthoDBiEOG7Z69C7.
    PhylomeDBiP08046.
    TreeFamiTF318980.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR021839. DUF3432.
    IPR021849. DUF3446.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF11914. DUF3432. 1 hit.
    PF11928. DUF3446. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG    50
    AAGTPEGSGG NSSSSTSSGG GGGGGSNSGS SAFNPQGEPS EQPYEHLTTE 100
    SFSDIALNNE KAMVETSYPS QTTRLPPITY TGRFSLEPAP NSGNTLWPEP 150
    LFSLVSGLVS MTNPPTSSSS APSPAASSSS SASQSPPLSC AVPSNDSSPI 200
    YSAAPTFPTP NTDIFPEPQS QAFPGSAGTA LQYPPPAYPA TKGGFQVPMI 250
    PDYLFPQQQG DLSLGTPDQK PFQGLENRTQ QPSLTPLSTI KAFATQSGSQ 300
    DLKALNTTYQ SQLIKPSRMR KYPNRPSKTP PHERPYACPV ESCDRRFSRS 350
    DELTRHIRIH TGQKPFQCRI CMRNFSRSDH LTTHIRTHTG EKPFACDICG 400
    RKFARSDERK RHTKIHLRQK DKKADKSVVA SPAASSLSSY PSPVATSYPS 450
    PATTSFPSPV PTSYSSPGSS TYPSPAHSGF PSPSVATTFA SVPPAFPTQV 500
    SSFPSAGVSS SFSTSTGLSD MTATFSPRTI EIC 533
    Length:533
    Mass (Da):56,590
    Last modified:November 1, 1990 - v2
    Checksum:i36E3935767CEAA0F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti76 – 761S → T(PubMed:2511075)Curated
    Sequence conflicti76 – 761S → T(PubMed:3133658)Curated
    Sequence conflicti80 – 801S → T(PubMed:2511075)Curated
    Sequence conflicti80 – 801S → T(PubMed:3133658)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20157 mRNA. Translation: AAA37544.1.
    M22326 mRNA. Translation: AAA40416.1.
    M28845, M28844 Genomic DNA. Translation: AAB00468.1.
    M19643 mRNA. Translation: AAA39382.1.
    CCDSiCCDS29136.1.
    PIRiJS0304.
    RefSeqiNP_031939.1. NM_007913.5.
    UniGeneiMm.181959.

    Genome annotation databases

    EnsembliENSMUST00000064795; ENSMUSP00000069616; ENSMUSG00000038418.
    ENSMUST00000165033; ENSMUSP00000126931; ENSMUSG00000038418.
    GeneIDi13653.
    KEGGimmu:13653.
    UCSCiuc008elt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20157 mRNA. Translation: AAA37544.1 .
    M22326 mRNA. Translation: AAA40416.1 .
    M28845 , M28844 Genomic DNA. Translation: AAB00468.1 .
    M19643 mRNA. Translation: AAA39382.1 .
    CCDSi CCDS29136.1.
    PIRi JS0304.
    RefSeqi NP_031939.1. NM_007913.5.
    UniGenei Mm.181959.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A1F X-ray 2.10 A 333-421 [» ]
    1A1G X-ray 1.90 A 333-421 [» ]
    1A1H X-ray 1.60 A 333-421 [» ]
    1A1I X-ray 1.60 A 333-421 [» ]
    1A1J X-ray 2.00 A 333-421 [» ]
    1A1K X-ray 1.90 A 333-421 [» ]
    1A1L X-ray 2.30 A 333-421 [» ]
    1AAY X-ray 1.60 A 333-421 [» ]
    1F2I X-ray 2.35 G/H/I/J/K/L 331-389 [» ]
    1G2D X-ray 2.20 C/F 333-421 [» ]
    1G2F X-ray 2.00 C/F 333-421 [» ]
    1JK1 X-ray 1.90 A 333-421 [» ]
    1JK2 X-ray 1.65 A 333-421 [» ]
    1LLM X-ray 1.50 C/D 364-412 [» ]
    1P47 X-ray 2.20 A/B 333-419 [» ]
    1ZAA X-ray 2.10 C 332-418 [» ]
    ProteinModelPortali P08046.
    SMRi P08046. Positions 334-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199404. 7 interactions.

    PTM databases

    PhosphoSitei P08046.

    Proteomic databases

    PRIDEi P08046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064795 ; ENSMUSP00000069616 ; ENSMUSG00000038418 .
    ENSMUST00000165033 ; ENSMUSP00000126931 ; ENSMUSG00000038418 .
    GeneIDi 13653.
    KEGGi mmu:13653.
    UCSCi uc008elt.1. mouse.

    Organism-specific databases

    CTDi 1958.
    MGIi MGI:95295. Egr1.

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000036856.
    HOVERGENi HBG003909.
    InParanoidi P08046.
    KOi K09203.
    OMAi AVTNSFS.
    OrthoDBi EOG7Z69C7.
    PhylomeDBi P08046.
    TreeFami TF318980.

    Miscellaneous databases

    EvolutionaryTracei P08046.
    NextBioi 284384.
    PROi P08046.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08046.
    Bgeei P08046.
    CleanExi MM_EGR1.
    Genevestigatori P08046.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR021839. DUF3432.
    IPR021849. DUF3446.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF11914. DUF3432. 1 hit.
    PF11928. DUF3446. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization."
      Sukhatme V.P., Cao X., Chang L.C., Tsai-Morris C.-H., Stamenkovich D., Ferreira P.C.P., Cohen D.R., Edwards S.A., Shows T.B., Curran T., le Beau M.M., Adamson E.D.
      Cell 53:37-43(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with 'zinc finger' sequences."
      Christy B.A., Lau L.F., Nathans D.
      Proc. Natl. Acad. Sci. U.S.A. 85:7857-7861(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Structure, chromosome mapping and regulation of the mouse zinc-finger gene Krox-24; evidence for a common regulatory pathway for immediate-early serum-response genes."
      Janssen-Timmen U., Lemaire P., Mattei M.-G., Revelant O., Charnay P.
      Gene 80:325-336(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells."
      Lemaire P., Revelant O., Bravo R., Charnay P.
      Proc. Natl. Acad. Sci. U.S.A. 85:4691-4695(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-533.
    5. "In vivo mutational analysis of the NGFI-A zinc fingers."
      Wilson T.E., Day M.L., Pexton T., Padgett K.A., Johnston M., Milbrandt J.
      J. Biol. Chem. 267:3718-3724(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ZINC-FINGERS.
    6. "Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A."
      Pavletich N.P., Pabo C.O.
      Science 252:809-817(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 333-421.
    7. "High-resolution structures of variant Zif268-DNA complexes: implications for understanding zinc finger-DNA recognition."
      Elrod-Erickson M., Benson T.E., Pabo C.O.
      Structure 6:451-464(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 333-421 WITH VARIATIONS.

    Entry informationi

    Entry nameiEGR1_MOUSE
    AccessioniPrimary (citable) accession number: P08046
    Secondary accession number(s): Q61777
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 150 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3