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P08046

- EGR1_MOUSE

UniProt

P08046 - EGR1_MOUSE

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Protein

Early growth response protein 1

Gene

Egr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri336 – 36025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri366 – 38823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri394 – 41623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. double-stranded DNA binding Source: Ensembl
  2. metal ion binding Source: UniProtKB-KW
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  5. RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
  6. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  7. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  8. transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

  1. BMP signaling pathway Source: MGI
  2. cellular response to antibiotic Source: Ensembl
  3. cellular response to cAMP Source: RefGenome
  4. cellular response to drug Source: Ensembl
  5. cellular response to follicle-stimulating hormone stimulus Source: Ensembl
  6. cellular response to gamma radiation Source: BHF-UCL
  7. cellular response to gonadotropin stimulus Source: RefGenome
  8. cellular response to growth factor stimulus Source: Ensembl
  9. cellular response to heparin Source: UniProtKB
  10. cellular response to hyperoxia Source: Ensembl
  11. cellular response to hypoxia Source: Ensembl
  12. cellular response to insulin stimulus Source: Ensembl
  13. cellular response to isoquinoline alkaloid Source: Ensembl
  14. cellular response to mechanical stimulus Source: Ensembl
  15. cellular response to mycophenolic acid Source: UniProtKB
  16. cellular response to organic substance Source: MGI
  17. cellular response to steroid hormone stimulus Source: Ensembl
  18. circadian rhythm Source: Ensembl
  19. glomerular mesangial cell proliferation Source: UniProtKB
  20. interleukin-1-mediated signaling pathway Source: Ensembl
  21. learning or memory Source: Ensembl
  22. long-term synaptic potentiation Source: Ensembl
  23. negative regulation of apoptotic process Source: RefGenome
  24. negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  25. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  26. oligodendrocyte differentiation Source: Ensembl
  27. positive regulation of glomerular metanephric mesangial cell proliferation Source: UniProtKB
  28. positive regulation of neuron apoptotic process Source: Ensembl
  29. positive regulation of transcription, DNA-templated Source: UniProtKB
  30. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  31. regulation of long-term neuronal synaptic plasticity Source: RefGenome
  32. regulation of protein sumoylation Source: Ensembl
  33. regulation of transcription, DNA-templated Source: MGI
  34. response to amphetamine Source: Ensembl
  35. response to carbon monoxide Source: Ensembl
  36. response to cocaine Source: Ensembl
  37. response to electrical stimulus Source: Ensembl
  38. response to ethanol Source: Ensembl
  39. response to glucose Source: UniProtKB
  40. response to insulin Source: UniProtKB
  41. response to norepinephrine Source: Ensembl
  42. response to nutrient levels Source: Ensembl
  43. skeletal muscle cell differentiation Source: MGI
  44. T cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 1
Short name:
EGR-1
Alternative name(s):
Nerve growth factor-induced protein A
Short name:
NGFI-A
Transcription factor Zif268
Zinc finger protein Krox-24
Gene namesi
Name:Egr1
Synonyms:Egr-1, Krox-24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:95295. Egr1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Early growth response protein 1PRO_0000047110Add
BLAST

Proteomic databases

MaxQBiP08046.
PRIDEiP08046.

PTM databases

PhosphoSiteiP08046.

Expressioni

Inductioni

By growth factors.

Gene expression databases

BgeeiP08046.
CleanExiMM_EGR1.
ExpressionAtlasiP08046. baseline and differential.
GenevestigatoriP08046.

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.By similarity

Protein-protein interaction databases

BioGridi199404. 7 interactions.

Structurei

Secondary structure

1
533
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi335 – 3373Combined sources
Beta strandi340 – 3423Combined sources
Beta strandi346 – 3494Combined sources
Helixi350 – 36112Combined sources
Turni369 – 3713Combined sources
Beta strandi374 – 3763Combined sources
Helixi378 – 38912Combined sources
Turni397 – 3993Combined sources
Beta strandi402 – 4054Combined sources
Helixi406 – 4127Combined sources
Helixi414 – 4174Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A1FX-ray2.10A333-421[»]
1A1GX-ray1.90A333-421[»]
1A1HX-ray1.60A333-421[»]
1A1IX-ray1.60A333-421[»]
1A1JX-ray2.00A333-421[»]
1A1KX-ray1.90A333-421[»]
1A1LX-ray2.30A333-421[»]
1AAYX-ray1.60A333-421[»]
1F2IX-ray2.35G/H/I/J/K/L331-389[»]
1G2DX-ray2.20C/F333-421[»]
1G2FX-ray2.00C/F333-421[»]
1JK1X-ray1.90A333-421[»]
1JK2X-ray1.65A333-421[»]
1LLMX-ray1.50C/D364-412[»]
1P47X-ray2.20A/B333-419[»]
1ZAAX-ray2.10C332-418[»]
ProteinModelPortaliP08046.
SMRiP08046. Positions 334-453.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08046.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi57 – 8125Gly/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri336 – 36025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri366 – 38823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri394 – 41623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08046.
KOiK09203.
OMAiAVTNSFS.
OrthoDBiEOG7Z69C7.
PhylomeDBiP08046.
TreeFamiTF318980.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 1 hit.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08046-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG
60 70 80 90 100
AAGTPEGSGG NSSSSTSSGG GGGGGSNSGS SAFNPQGEPS EQPYEHLTTE
110 120 130 140 150
SFSDIALNNE KAMVETSYPS QTTRLPPITY TGRFSLEPAP NSGNTLWPEP
160 170 180 190 200
LFSLVSGLVS MTNPPTSSSS APSPAASSSS SASQSPPLSC AVPSNDSSPI
210 220 230 240 250
YSAAPTFPTP NTDIFPEPQS QAFPGSAGTA LQYPPPAYPA TKGGFQVPMI
260 270 280 290 300
PDYLFPQQQG DLSLGTPDQK PFQGLENRTQ QPSLTPLSTI KAFATQSGSQ
310 320 330 340 350
DLKALNTTYQ SQLIKPSRMR KYPNRPSKTP PHERPYACPV ESCDRRFSRS
360 370 380 390 400
DELTRHIRIH TGQKPFQCRI CMRNFSRSDH LTTHIRTHTG EKPFACDICG
410 420 430 440 450
RKFARSDERK RHTKIHLRQK DKKADKSVVA SPAASSLSSY PSPVATSYPS
460 470 480 490 500
PATTSFPSPV PTSYSSPGSS TYPSPAHSGF PSPSVATTFA SVPPAFPTQV
510 520 530
SSFPSAGVSS SFSTSTGLSD MTATFSPRTI EIC
Length:533
Mass (Da):56,590
Last modified:November 1, 1990 - v2
Checksum:i36E3935767CEAA0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761S → T(PubMed:2511075)Curated
Sequence conflicti76 – 761S → T(PubMed:3133658)Curated
Sequence conflicti80 – 801S → T(PubMed:2511075)Curated
Sequence conflicti80 – 801S → T(PubMed:3133658)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20157 mRNA. Translation: AAA37544.1.
M22326 mRNA. Translation: AAA40416.1.
M28845, M28844 Genomic DNA. Translation: AAB00468.1.
M19643 mRNA. Translation: AAA39382.1.
CCDSiCCDS29136.1.
PIRiJS0304.
RefSeqiNP_031939.1. NM_007913.5.
UniGeneiMm.181959.

Genome annotation databases

EnsembliENSMUST00000064795; ENSMUSP00000069616; ENSMUSG00000038418.
ENSMUST00000165033; ENSMUSP00000126931; ENSMUSG00000038418.
GeneIDi13653.
KEGGimmu:13653.
UCSCiuc008elt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20157 mRNA. Translation: AAA37544.1 .
M22326 mRNA. Translation: AAA40416.1 .
M28845 , M28844 Genomic DNA. Translation: AAB00468.1 .
M19643 mRNA. Translation: AAA39382.1 .
CCDSi CCDS29136.1.
PIRi JS0304.
RefSeqi NP_031939.1. NM_007913.5.
UniGenei Mm.181959.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A1F X-ray 2.10 A 333-421 [» ]
1A1G X-ray 1.90 A 333-421 [» ]
1A1H X-ray 1.60 A 333-421 [» ]
1A1I X-ray 1.60 A 333-421 [» ]
1A1J X-ray 2.00 A 333-421 [» ]
1A1K X-ray 1.90 A 333-421 [» ]
1A1L X-ray 2.30 A 333-421 [» ]
1AAY X-ray 1.60 A 333-421 [» ]
1F2I X-ray 2.35 G/H/I/J/K/L 331-389 [» ]
1G2D X-ray 2.20 C/F 333-421 [» ]
1G2F X-ray 2.00 C/F 333-421 [» ]
1JK1 X-ray 1.90 A 333-421 [» ]
1JK2 X-ray 1.65 A 333-421 [» ]
1LLM X-ray 1.50 C/D 364-412 [» ]
1P47 X-ray 2.20 A/B 333-419 [» ]
1ZAA X-ray 2.10 C 332-418 [» ]
ProteinModelPortali P08046.
SMRi P08046. Positions 334-453.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199404. 7 interactions.

PTM databases

PhosphoSitei P08046.

Proteomic databases

MaxQBi P08046.
PRIDEi P08046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000064795 ; ENSMUSP00000069616 ; ENSMUSG00000038418 .
ENSMUST00000165033 ; ENSMUSP00000126931 ; ENSMUSG00000038418 .
GeneIDi 13653.
KEGGi mmu:13653.
UCSCi uc008elt.1. mouse.

Organism-specific databases

CTDi 1958.
MGIi MGI:95295. Egr1.

Phylogenomic databases

eggNOGi COG5048.
HOGENOMi HOG000036856.
HOVERGENi HBG003909.
InParanoidi P08046.
KOi K09203.
OMAi AVTNSFS.
OrthoDBi EOG7Z69C7.
PhylomeDBi P08046.
TreeFami TF318980.

Miscellaneous databases

ChiTaRSi Egr1. mouse.
EvolutionaryTracei P08046.
NextBioi 284384.
PROi P08046.
SOURCEi Search...

Gene expression databases

Bgeei P08046.
CleanExi MM_EGR1.
ExpressionAtlasi P08046. baseline and differential.
Genevestigatori P08046.

Family and domain databases

Gene3Di 3.30.160.60. 3 hits.
InterProi IPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF11914. DUF3432. 1 hit.
PF11928. DUF3446. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 3 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization."
    Sukhatme V.P., Cao X., Chang L.C., Tsai-Morris C.-H., Stamenkovich D., Ferreira P.C.P., Cohen D.R., Edwards S.A., Shows T.B., Curran T., le Beau M.M., Adamson E.D.
    Cell 53:37-43(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with 'zinc finger' sequences."
    Christy B.A., Lau L.F., Nathans D.
    Proc. Natl. Acad. Sci. U.S.A. 85:7857-7861(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure, chromosome mapping and regulation of the mouse zinc-finger gene Krox-24; evidence for a common regulatory pathway for immediate-early serum-response genes."
    Janssen-Timmen U., Lemaire P., Mattei M.-G., Revelant O., Charnay P.
    Gene 80:325-336(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells."
    Lemaire P., Revelant O., Bravo R., Charnay P.
    Proc. Natl. Acad. Sci. U.S.A. 85:4691-4695(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-533.
  5. "In vivo mutational analysis of the NGFI-A zinc fingers."
    Wilson T.E., Day M.L., Pexton T., Padgett K.A., Johnston M., Milbrandt J.
    J. Biol. Chem. 267:3718-3724(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ZINC-FINGERS.
  6. "Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A."
    Pavletich N.P., Pabo C.O.
    Science 252:809-817(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 333-421.
  7. "High-resolution structures of variant Zif268-DNA complexes: implications for understanding zinc finger-DNA recognition."
    Elrod-Erickson M., Benson T.E., Pabo C.O.
    Structure 6:451-464(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 333-421 WITH VARIATIONS.

Entry informationi

Entry nameiEGR1_MOUSE
AccessioniPrimary (citable) accession number: P08046
Secondary accession number(s): Q61777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1990
Last modified: November 26, 2014
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3