P08038 (DRNE_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular deoxyribonuclease
Short name=DNase
EC=3.1.21.-

Gene names

Name:	dns
Ordered Locus Names:	VC_0470

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Sequence similarities	Belongs to the EndA/NucM nuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA catabolic process Inferred from mutant phenotype PubMed 1791765. Source: TIGR
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	deoxyribonuclease activity Inferred from mutant phenotype PubMed 1791765. Source: TIGR endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Potential

Chain

21 – 231

211

Extracellular deoxyribonuclease

PRO_0000007833

Experimental info

Sequence conflict

50

1

R → P in AAA27516. Ref.1

Sequence conflict

163 – 169

7

PPERARG → AQSVQR in AAA27516. Ref.1

Sequence conflict

191

1

S → T in AAA27516. Ref.1

Sequence conflict

223 – 228

6

RFVREQ → LLCACK in AAA27516. Ref.1

Secondary structure

1

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231

Helix Strand Turn

Details...

Helix

26 – 36

11

Turn

43 – 45

3

Beta strand

48 – 52

5

Beta strand

55 – 59

5

Helix

61 – 63

3

Helix

71 – 74

4

Beta strand

76 – 83

8

Helix

85 – 89

5

Helix

93 – 106

14

Helix

108 – 115

8

Helix

117 – 119

3

Beta strand

120 – 124

5

Helix

125 – 131

7

Beta strand

144 – 148

5

Beta strand

150 – 155

6

Turn

156 – 159

4

Beta strand

160 – 162

3

Helix

165 – 182

18

Helix

188 – 200

13

Helix

205 – 218

14

Helix

223 – 226

4

Sequences

	Sequence		Length	Mass (Da)
	P08038 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 9E9541460EDE866B	FASTA	231	26,713
	10 20 30 40 50 60 MMIFRFVTTL AASLPLLTFA APISFSHAKN EAVKIYRDHP VSFYCGCEIR WQGKKGIPDL 70 80 90 100 110 120 ESCGYQVRKN ENRASRIEWE HVVPAWQFGH QLQCWQQGGR KNCTRTSPEF NQMEADLHNL 130 140 150 160 170 180 TPAIGEVNGD RSNFSFSQWN GVDGVTYGQC EMQVNFKERT AMPPERARGA IARTYLYMSE 190 200 210 220 230 QYGLRLSKAQ SQLMQAWNNQ YPVSEWECVR DQRIEKVQGN SNRFVREQCP N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Extracellular proteins of Vibrio cholerae: molecular cloning, nucleotide sequence and characterization of the deoxyribonuclease (DNase) together with its periplasmic localization in Escherichia coli K-12." Focareta T., Manning P.A. Gene 53:31-40(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Manning P.A. Submitted (NOV-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M16499 Genomic DNA. Translation: AAA27516.1.
AE003852 Genomic DNA. Translation: AAF93643.1.

PIR

A26509.
C82319.

RefSeq

NP_230124.1. NC_002505.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VND	X-ray	1.70	A	1-231	[»]

ProteinModelPortal

P08038.

SMR

P08038. Positions 24-231.

ModBase

Search...

Protein-protein interaction databases

STRING

243277.VC0470.

Protocols and materials databases

DNASU

2615132.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAF93643; AAF93643; VC_0470.

GeneID

2615132.

KEGG

vch:VC0470.

PATRIC

20080035. VBIVibCho83274_0443.

Phylogenomic databases

eggNOG

COG2356.

KO

K01150.

OMA

IECKRDN.

ProtClustDB

CLSK793636.

Family and domain databases

InterPro

IPR007346. Endonuclease-I.
[Graphical view]

Pfam

PF04231. Endonuclease_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P08038.

Entry information

Entry name

DRNE_VIBCH

Accession

Primary (citable) accession number: P08038
Secondary accession number(s): Q9KUP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program