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Protein

Gap junction beta-1 protein

Gene

Gjb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Molecular functioni

  • gap junction channel activity Source: RGD
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

  • cell communication Source: RGD
  • epididymis development Source: RGD
  • protein oligomerization Source: RGD
  • purine ribonucleotide transport Source: RGD

Enzyme and pathway databases

ReactomeiR-RNO-190704. Oligomerization of connexins into connexons.
R-RNO-190861. Gap junction assembly.

Protein family/group databases

TCDBi1.A.24.1.2. the gap junction-forming connexin (connexin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction beta-1 protein
Alternative name(s):
Connexin-32
Short name:
Cx32
GAP junction 28 kDa liver protein
Gene namesi
Name:Gjb1
Synonyms:Cxn-32
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi61926. Gjb1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 45HelicalSequence analysisAdd BLAST23
Topological domaini46 – 75ExtracellularSequence analysisAdd BLAST30
Transmembranei76 – 95HelicalSequence analysisAdd BLAST20
Topological domaini96 – 130CytoplasmicSequence analysisAdd BLAST35
Transmembranei131 – 153HelicalSequence analysisAdd BLAST23
Topological domaini154 – 191ExtracellularSequence analysisAdd BLAST38
Transmembranei192 – 214HelicalSequence analysisAdd BLAST23
Topological domaini215 – 283CytoplasmicSequence analysisAdd BLAST69

GO - Cellular componenti

  • connexin complex Source: InterPro
  • endoplasmic reticulum Source: RGD
  • gap junction Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • lateral plasma membrane Source: RGD
  • plasma membrane Source: RGD

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000578521 – 283Gap junction beta-1 proteinAdd BLAST283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08033.
PRIDEiP08033.

PTM databases

iPTMnetiP08033.
PhosphoSitePlusiP08033.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003746.
ExpressionAtlasiP08033. baseline and differential.
GenevisibleiP08033. RN.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. Interacts with CNST (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248217. 1 interactor.
STRINGi10116.ENSRNOP00000004979.

Structurei

3D structure databases

ProteinModelPortaliP08033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFM8. Eukaryota.
ENOG410Y7VN. LUCA.
GeneTreeiENSGT00860000133694.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP08033.
KOiK07620.
OMAiYLVVRAC.
OrthoDBiEOG091G0FKH.
PhylomeDBiP08033.
TreeFamiTF329606.

Family and domain databases

InterProiView protein in InterPro
IPR000500. Connexin.
IPR002267. Connexin32.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
PANTHERiPTHR11984. PTHR11984. 1 hit.
PTHR11984:SF99. PTHR11984:SF99. 1 hit.
PfamiView protein in Pfam
PF00029. Connexin. 1 hit.
PRINTSiPR00206. CONNEXIN.
PR01138. CONNEXINB1.
SMARTiView protein in SMART
SM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
PROSITEiView protein in PROSITE
PS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P08033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS
60 70 80 90 100
FICNTLQPGC NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH
110 120 130 140 150
IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM
160 170 180 190 200
YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF VSRPTEKTVF TVFMLAASGI
210 220 230 240 250
CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK
260 270 280
LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC
Length:283
Mass (Da):32,004
Last modified:August 1, 1988 - v1
Checksum:iC79FC46AA13BC5D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04070 mRNA. Translation: CAA27705.1.
X04303 mRNA. Translation: CAA27846.1.
M23565 Genomic DNA. Translation: AAA81569.1.
BC078868 mRNA. Translation: AAH78868.1.
AH003192 Genomic DNA. Translation: AAA75195.1.
PIRiA92745. GJRT.
I52552.
RefSeqiNP_058947.1. NM_017251.2.
XP_008771477.1. XM_008773255.2.
XP_017457434.1. XM_017601945.1.
UniGeneiRn.10444.

Genome annotation databases

EnsembliENSRNOT00000076816; ENSRNOP00000068248; ENSRNOG00000003746.
GeneIDi29584.
KEGGirno:29584.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCXB1_RAT
AccessioniPrimary (citable) accession number: P08033
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 10, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families