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P08032

- SPTA1_MOUSE

UniProt

P08032 - SPTA1_MOUSE

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Protein
Spectrin alpha chain, erythrocytic 1
Gene
Spta1, Spna1, Spta
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi2280 – 2291121 Reviewed prediction
Add
BLAST
Calcium bindingi2323 – 2334122 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. protein heterodimerization activity Source: MGI

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. actin filament capping Source: UniProtKB-KW
  3. hemopoiesis Source: MGI
  4. lymphocyte homeostasis Source: MGI
  5. plasma membrane organization Source: MGI
  6. porphyrin-containing compound biosynthetic process Source: MGI
  7. positive regulation of T cell proliferation Source: MGI
  8. positive regulation of protein binding Source: MGI
  9. regulation of cell shape Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Actin capping

Keywords - Biological processi

Cell shape

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Spectrin alpha chain, erythrocytic 1
Alternative name(s):
Erythroid alpha-spectrin
Gene namesi
Name:Spta1
Synonyms:Spna1, Spta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:98385. Spta1.

Subcellular locationi

GO - Cellular componenti

  1. cortical cytoskeleton Source: MGI
  2. cuticular plate Source: MGI
  3. membrane Source: MGI
  4. spectrin Source: MGI
  5. spectrin-associated cytoskeleton Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24152415Spectrin alpha chain, erythrocytic 1
PRO_0000073453Add
BLAST

Proteomic databases

MaxQBiP08032.
PaxDbiP08032.
PRIDEiP08032.

PTM databases

PhosphoSiteiP08032.

Expressioni

Gene expression databases

BgeeiP08032.
CleanExiMM_SPNA1.
GenevestigatoriP08032.

Interactioni

Subunit structurei

Composed of non-homologous chains, alpha and beta, which aggregate to form dimers, tetramers, and higher polymers. Interacts with FASLG By similarity.

Protein-protein interaction databases

BioGridi203458. 5 interactions.
IntActiP08032. 2 interactions.
MINTiMINT-85326.

Structurei

3D structure databases

ProteinModelPortaliP08032.
SMRiP08032. Positions 4-953, 992-1032, 1047-2260, 2263-2411.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 5033Spectrin 1
Add
BLAST
Repeati52 – 154103Spectrin 2
Add
BLAST
Repeati156 – 260105Spectrin 3
Add
BLAST
Repeati262 – 366105Spectrin 4
Add
BLAST
Repeati368 – 472105Spectrin 5
Add
BLAST
Repeati474 – 578105Spectrin 6
Add
BLAST
Repeati580 – 683104Spectrin 7
Add
BLAST
Repeati685 – 789105Spectrin 8
Add
BLAST
Repeati791 – 895105Spectrin 9
Add
BLAST
Repeati897 – 96670Spectrin 10
Add
BLAST
Domaini975 – 103460SH3
Add
BLAST
Repeati1080 – 1179100Spectrin 11
Add
BLAST
Repeati1181 – 1285105Spectrin 12
Add
BLAST
Repeati1287 – 1391105Spectrin 13
Add
BLAST
Repeati1393 – 1496104Spectrin 14
Add
BLAST
Repeati1498 – 1603106Spectrin 15
Add
BLAST
Repeati1605 – 1709105Spectrin 16
Add
BLAST
Repeati1711 – 1815105Spectrin 17
Add
BLAST
Repeati1817 – 1922106Spectrin 18
Add
BLAST
Repeati1924 – 2029106Spectrin 19
Add
BLAST
Repeati2039 – 2143105Spectrin 20
Add
BLAST
Repeati2153 – 2254102Spectrin 21
Add
BLAST
Domaini2267 – 230236EF-hand 1
Add
BLAST
Domaini2310 – 234536EF-hand 2
Add
BLAST
Domaini2347 – 238236EF-hand 3
Add
BLAST

Sequence similaritiesi

Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 21 spectrin repeats.

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG237318.
GeneTreeiENSGT00750000117361.
HOGENOMiHOG000246965.
HOVERGENiHBG059266.
InParanoidiB2RWX6.
KOiK06114.
OMAiSINKDWW.
OrthoDBiEOG7GXP9K.
PhylomeDBiP08032.
TreeFamiTF343803.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR001452. SH3_domain.
IPR018159. Spectrin/alpha-actinin.
IPR013315. Spectrin_alpha_SH3.
IPR002017. Spectrin_repeat.
[Graphical view]
PfamiPF08726. EFhand_Ca_insen. 1 hit.
PF00018. SH3_1. 1 hit.
PF00435. Spectrin. 20 hits.
[Graphical view]
PRINTSiPR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
SM00150. SPEC. 20 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08032-1 [UniParc]FASTAAdd to Basket

« Hide

METPKETAVE SSGPKVLETA EEIQHRRAEV LNQYQRFKDR VAERGQKLEE     50
SYHYQVFRRD ADDLEKWIME KLEIAKDKTY EPTNIQGKYQ KHESFVSEVQ 100
AKSRVLPELE EIREARFAED HFAHEATKTH LKQLRLLWDL LLELTQEKSD 150
VLLRALKFYQ YSQECEDILE WVKEKEAIVT LVELGDDWER TEVLHKKFEE 200
FQEELTARKG KVDRVNQYAN ECAQEKHPKL PEIKAKQDEV NAAWDRLWSL 250
ALKRRESLSN AADLQRFKRD VNEAIQWMEE KEPQLTSEDY GKDLVSSEAL 300
FHNHKRLERN LAVMDDKVKE LCAKADKLMI SHSADAPQIQ QMKLDLVSNW 350
ERIRALATNR YAKLKASYGY HRFLSDYDEL SGWMKEKTAL INADELPTDV 400
ASGEALLARH QQHKHEIDSY DDRFQSADAT GQELLDGNHE ASEEIREKMT 450
ILANDWAALL ELWDKCQHQY RQCLDFHLFY RDSEQVDSWM SRQEAFLENE 500
DLGNSVGSVE ALLQKHDDFE EAFTAQEEKI ITLDETATKL IDNDHYDSEN 550
IAAIRDGLLA RRDALRERAA TRRKLLVDSQ LLQQLYQDSD DLKTWINKKK 600
KLADDDDYKD VQNLKSRVQK QQDFEEELAV NEIMLNNLEK TGQEMIEDGH 650
YASEAVAARL SEVANLWKEL LEATAQKGTQ LYEANQLLQF ENNAEDLKRW 700
LEEVEWQVTS EDYGKGLADV QNLLRKHGLL ESDVTARQNQ VDTLTDMAAH 750
FEEIGHPDSG DIRARQESLL SRFEALKEPL AIRKKKLIDL LKLQQICRDS 800
EDEEAWIQET EPSAASTHLG KDLVAAKNLL NRHEVILADI ASHEPRIQVI 850
TERGNKMVEE GHFAAEDIAS RVESLNKNME SLHARAIRRE NDLKANVQLQ 900
QYLADLHEAE AWIKEKEPIV DNKNYGADEE AAGALLKKHE AFLVDLNAFE 950
NSIKALRDQA EVCQQQQAAP VDEAGREARV IALYDFEARS RREVSMKKND 1000
VLTLLSSINK DWWKVEADDH QGFVPAVYVR KLAPDELPGF PQHRQEEPVN 1050
IPQLQQQVET LYHSLLDRAE ERRRRLLQRY NEFLLAYEAG DMLEWIQEKK 1100
TENTGVELDD VWELQKKFDE FQRDLKSNEP RLKDINKVAD ELLFEELLTP 1150
EGAHIRQELN TRWNSLKRLA DEQYQLLSSA HAVEMFHREA DDVKEQIDKK 1200
CRALNAADPG SDLLSVQALQ RQHEVFERDI IPLGEKVTTL GETAERLCES 1250
HPDATEDLQK QRTELNEAWD TLQGLTSDRK ESLNEAHKFF LFLSKASDLE 1300
NWIKTIGGVI SSPELAEDLT GTEILLERHQ EHHDDIKRED PTFQALEDFG 1350
TELIDSGHRN RREIDNTLQN INSKRDNLEK SWENRKKMLD QCLELQLFRG 1400
KCDQVESWMV ARENSLRSDD RDHLNSLQAL MKKRDDLDKA ITAQEGKISD 1450
LENVATRLID NDHYAKEEIA ARLQRVLDRW KALKEQLLTE LGKLGDYADL 1500
KQFYRDLEDL EEWINEMLPI ACDESYKDPT NIQRKYLKHQ AFENEVNGRA 1550
EQVDGVINLG NSLIERRVCD GDEENMQEQL DKLKENWDYL LERTTDKGQK 1600
LNEASRQQRF NTSIRDFEFW LSEAEGLLAM KDQARDLTSA GNLLKKHQLL 1650
EAEMLAREDP LKDLNDLAQE LISSGTFNID QIEEKMNGVN ERFENVQSLA 1700
AAHHEKLKET YALFQFFQDL DDEEAWIEEK LLRVSSQDYG RDLQSVQNLL 1750
KKHKRLEGEL VAHEPAVQNV LDTAESLRDK AAVGKEEIQE RLAQFVQHWE 1800
KLKELAKTRG VNLEESLEYL QFMENAEEEE AWLGEKCALV SRGDSGDTLA 1850
ATQSLLKKHE ALENDFAVHK NRVQDVCAQG EDILNKEETQ NKDKISTKIQ 1900
VLNEKTASLA KALAAWKSQL DDVHAFQQFN WKADVVESWI GEKEASLKTK 1950
SNGADLTAFL TLLAKHDTLD ASLQSFQQER LSEIAELKDQ LVAGEHSQAK 2000
AIEEQHAALL RHWEQLLEAS RVHRQKLLEK QLPLQKAEEL FMEFAHKASA 2050
FNNWCENAEE DLSEPVHCVS LNEIRQLQKE HEAFLASLAG AQEDFNYLLE 2100
LDKQIKALNV PSSPYTWLTV DVLGRIWNHL PDIIKEREQE LQKEEARQIK 2150
NFEMCQEFEQ NASAFLQWIQ ETRAYFLDGS LLKETGTLES QLEANKRKQK 2200
EIQAMKRHLT KIEDLGDSME EALILDIKYS TIGLAQQWDQ LHQLGMRMQH 2250
NLEQQIQAKD TIGVSEETLK EFSTTYKHFD ENLTGRLTHK EFRSCLRGLN 2300
YYLPMVEEGE PEPKFEKFLN AVDPGRKGYV SLEDYTSFLI DKESENIKTS 2350
DDIESAFQAL AEGKAYITKE DMKQALTPEQ VSFCTIHMQQ YMDPRGRSQP 2400
AGYDYVGFTN SFFGN 2415
Length:2,415
Mass (Da):279,865
Last modified:July 7, 2009 - v3
Checksum:i510B61CDEC4FAD13
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371P → L in AAB47540. 1 Publication
Sequence conflicti501 – 5011D → Y in AAI50748. 1 Publication
Sequence conflicti716 – 7161G → V in AAB47540. 1 Publication
Sequence conflicti741 – 7411V → M in AAB47540. 1 Publication
Sequence conflicti741 – 7411V → M in AAI50748. 1 Publication
Sequence conflicti1044 – 10452RQ → QR in AAB47540. 1 Publication
Sequence conflicti1044 – 10452RQ → QR in AAI50748. 1 Publication
Sequence conflicti1225 – 12251V → C in AAB47540. 1 Publication
Sequence conflicti1238 – 12381T → N in AAB47540. 1 Publication
Sequence conflicti1425 – 14251N → D in AAB47540. 1 Publication
Sequence conflicti1582 – 15821K → M in AAB47540. 1 Publication
Sequence conflicti2062 – 20621L → M in AAB47540. 1 Publication
Sequence conflicti2062 – 20621L → M in AAI50748. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87455 mRNA. Translation: AAB47540.1.
AF093576 mRNA. Translation: AAC61874.1.
AC113483 mRNA. No translation available.
AC156549 mRNA. No translation available.
BC150747 mRNA. Translation: AAI50748.1.
M10276 mRNA. Translation: AAA40123.1.
CCDSiCCDS35795.1.
PIRiA05283.
RefSeqiNP_035595.2. NM_011465.4.
UniGeneiMm.200611.

Genome annotation databases

EnsembliENSMUST00000027817; ENSMUSP00000027817; ENSMUSG00000026532.
GeneIDi20739.
KEGGimmu:20739.
UCSCiuc007dsw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87455 mRNA. Translation: AAB47540.1 .
AF093576 mRNA. Translation: AAC61874.1 .
AC113483 mRNA. No translation available.
AC156549 mRNA. No translation available.
BC150747 mRNA. Translation: AAI50748.1 .
M10276 mRNA. Translation: AAA40123.1 .
CCDSi CCDS35795.1.
PIRi A05283.
RefSeqi NP_035595.2. NM_011465.4.
UniGenei Mm.200611.

3D structure databases

ProteinModelPortali P08032.
SMRi P08032. Positions 4-953, 992-1032, 1047-2260, 2263-2411.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203458. 5 interactions.
IntActi P08032. 2 interactions.
MINTi MINT-85326.

PTM databases

PhosphoSitei P08032.

Proteomic databases

MaxQBi P08032.
PaxDbi P08032.
PRIDEi P08032.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027817 ; ENSMUSP00000027817 ; ENSMUSG00000026532 .
GeneIDi 20739.
KEGGi mmu:20739.
UCSCi uc007dsw.2. mouse.

Organism-specific databases

CTDi 6708.
MGIi MGI:98385. Spta1.

Phylogenomic databases

eggNOGi NOG237318.
GeneTreei ENSGT00750000117361.
HOGENOMi HOG000246965.
HOVERGENi HBG059266.
InParanoidi B2RWX6.
KOi K06114.
OMAi SINKDWW.
OrthoDBi EOG7GXP9K.
PhylomeDBi P08032.
TreeFami TF343803.

Miscellaneous databases

NextBioi 299373.
PROi P08032.
SOURCEi Search...

Gene expression databases

Bgeei P08032.
CleanExi MM_SPNA1.
Genevestigatori P08032.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR001452. SH3_domain.
IPR018159. Spectrin/alpha-actinin.
IPR013315. Spectrin_alpha_SH3.
IPR002017. Spectrin_repeat.
[Graphical view ]
Pfami PF08726. EFhand_Ca_insen. 1 hit.
PF00018. SH3_1. 1 hit.
PF00435. Spectrin. 20 hits.
[Graphical view ]
PRINTSi PR01887. SPECTRNALPHA.
SMARTi SM00326. SH3. 1 hit.
SM00150. SPEC. 20 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50222. EF_HAND_2. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Burnett R.C., Avery A.C., Swardson C.J.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. Birkenmeier C.S., Gifford E.J., Barker J.E.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Sequence comparison of human and murine erythrocyte alpha-spectrin cDNA."
    Curtis P.J., Palumbo A., Ming J., Fraser P.J., Cioe L., Meo P., Shane S., Rovera G.
    Gene 36:357-362(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1567-1819.

Entry informationi

Entry nameiSPTA1_MOUSE
AccessioniPrimary (citable) accession number: P08032
Secondary accession number(s): B2RWX6, P97502
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This complex is anchored to the cytoplasmic face of the plasma membrane via another protein, ankyrin, which binds to beta-spectrin and mediates the binding of the whole complex to a transmembrane protein band 3. The interaction of erythrocyte spectrin with other proteins through specific binding domains lead to the formation of an extensive subplasmalemmal meshwork which is thought to be responsible for the maintenance of the biconcave shape of human erythrocytes, for the regulation of plasma membrane components and for the maintenance of the lipid asymmetry of the plasma membrane.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi