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P08032

- SPTA1_MOUSE

UniProt

P08032 - SPTA1_MOUSE

Protein

Spectrin alpha chain, erythrocytic 1

Gene

Spta1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi2280 – 2291121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi2323 – 2334122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. protein heterodimerization activity Source: MGI

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. actin filament capping Source: UniProtKB-KW
    3. hemopoiesis Source: MGI
    4. lymphocyte homeostasis Source: MGI
    5. plasma membrane organization Source: MGI
    6. porphyrin-containing compound biosynthetic process Source: MGI
    7. positive regulation of protein binding Source: MGI
    8. positive regulation of T cell proliferation Source: MGI
    9. regulation of cell shape Source: MGI

    Keywords - Molecular functioni

    Actin capping

    Keywords - Biological processi

    Cell shape

    Keywords - Ligandi

    Actin-binding, Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spectrin alpha chain, erythrocytic 1
    Alternative name(s):
    Erythroid alpha-spectrin
    Gene namesi
    Name:Spta1
    Synonyms:Spna1, Spta
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:98385. Spta1.

    Subcellular locationi

    GO - Cellular componenti

    1. cortical cytoskeleton Source: MGI
    2. cuticular plate Source: MGI
    3. membrane Source: MGI
    4. spectrin Source: MGI
    5. spectrin-associated cytoskeleton Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 24152415Spectrin alpha chain, erythrocytic 1PRO_0000073453Add
    BLAST

    Proteomic databases

    MaxQBiP08032.
    PaxDbiP08032.
    PRIDEiP08032.

    PTM databases

    PhosphoSiteiP08032.

    Expressioni

    Gene expression databases

    BgeeiP08032.
    CleanExiMM_SPNA1.
    GenevestigatoriP08032.

    Interactioni

    Subunit structurei

    Composed of non-homologous chains, alpha and beta, which aggregate to form dimers, tetramers, and higher polymers. Interacts with FASLG By similarity.By similarity

    Protein-protein interaction databases

    BioGridi203458. 5 interactions.
    IntActiP08032. 2 interactions.
    MINTiMINT-85326.

    Structurei

    3D structure databases

    ProteinModelPortaliP08032.
    SMRiP08032. Positions 4-953, 992-1032, 1047-2260, 2263-2411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati18 – 5033Spectrin 1Add
    BLAST
    Repeati52 – 154103Spectrin 2Add
    BLAST
    Repeati156 – 260105Spectrin 3Add
    BLAST
    Repeati262 – 366105Spectrin 4Add
    BLAST
    Repeati368 – 472105Spectrin 5Add
    BLAST
    Repeati474 – 578105Spectrin 6Add
    BLAST
    Repeati580 – 683104Spectrin 7Add
    BLAST
    Repeati685 – 789105Spectrin 8Add
    BLAST
    Repeati791 – 895105Spectrin 9Add
    BLAST
    Repeati897 – 96670Spectrin 10Add
    BLAST
    Domaini975 – 103460SH3PROSITE-ProRule annotationAdd
    BLAST
    Repeati1080 – 1179100Spectrin 11Add
    BLAST
    Repeati1181 – 1285105Spectrin 12Add
    BLAST
    Repeati1287 – 1391105Spectrin 13Add
    BLAST
    Repeati1393 – 1496104Spectrin 14Add
    BLAST
    Repeati1498 – 1603106Spectrin 15Add
    BLAST
    Repeati1605 – 1709105Spectrin 16Add
    BLAST
    Repeati1711 – 1815105Spectrin 17Add
    BLAST
    Repeati1817 – 1922106Spectrin 18Add
    BLAST
    Repeati1924 – 2029106Spectrin 19Add
    BLAST
    Repeati2039 – 2143105Spectrin 20Add
    BLAST
    Repeati2153 – 2254102Spectrin 21Add
    BLAST
    Domaini2267 – 230236EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini2310 – 234536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini2347 – 238236EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the spectrin family.Curated
    Contains 3 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation
    Contains 21 spectrin repeats.Curated

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiNOG237318.
    GeneTreeiENSGT00750000117361.
    HOGENOMiHOG000246965.
    HOVERGENiHBG059266.
    InParanoidiB2RWX6.
    KOiK06114.
    OMAiSINKDWW.
    OrthoDBiEOG7GXP9K.
    PhylomeDBiP08032.
    TreeFamiTF343803.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR002048. EF_hand_dom.
    IPR001452. SH3_domain.
    IPR018159. Spectrin/alpha-actinin.
    IPR013315. Spectrin_alpha_SH3.
    IPR002017. Spectrin_repeat.
    [Graphical view]
    PfamiPF08726. EFhand_Ca_insen. 1 hit.
    PF00018. SH3_1. 1 hit.
    PF00435. Spectrin. 20 hits.
    [Graphical view]
    PRINTSiPR01887. SPECTRNALPHA.
    SMARTiSM00326. SH3. 1 hit.
    SM00150. SPEC. 20 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50222. EF_HAND_2. 3 hits.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08032-1 [UniParc]FASTAAdd to Basket

    « Hide

    METPKETAVE SSGPKVLETA EEIQHRRAEV LNQYQRFKDR VAERGQKLEE     50
    SYHYQVFRRD ADDLEKWIME KLEIAKDKTY EPTNIQGKYQ KHESFVSEVQ 100
    AKSRVLPELE EIREARFAED HFAHEATKTH LKQLRLLWDL LLELTQEKSD 150
    VLLRALKFYQ YSQECEDILE WVKEKEAIVT LVELGDDWER TEVLHKKFEE 200
    FQEELTARKG KVDRVNQYAN ECAQEKHPKL PEIKAKQDEV NAAWDRLWSL 250
    ALKRRESLSN AADLQRFKRD VNEAIQWMEE KEPQLTSEDY GKDLVSSEAL 300
    FHNHKRLERN LAVMDDKVKE LCAKADKLMI SHSADAPQIQ QMKLDLVSNW 350
    ERIRALATNR YAKLKASYGY HRFLSDYDEL SGWMKEKTAL INADELPTDV 400
    ASGEALLARH QQHKHEIDSY DDRFQSADAT GQELLDGNHE ASEEIREKMT 450
    ILANDWAALL ELWDKCQHQY RQCLDFHLFY RDSEQVDSWM SRQEAFLENE 500
    DLGNSVGSVE ALLQKHDDFE EAFTAQEEKI ITLDETATKL IDNDHYDSEN 550
    IAAIRDGLLA RRDALRERAA TRRKLLVDSQ LLQQLYQDSD DLKTWINKKK 600
    KLADDDDYKD VQNLKSRVQK QQDFEEELAV NEIMLNNLEK TGQEMIEDGH 650
    YASEAVAARL SEVANLWKEL LEATAQKGTQ LYEANQLLQF ENNAEDLKRW 700
    LEEVEWQVTS EDYGKGLADV QNLLRKHGLL ESDVTARQNQ VDTLTDMAAH 750
    FEEIGHPDSG DIRARQESLL SRFEALKEPL AIRKKKLIDL LKLQQICRDS 800
    EDEEAWIQET EPSAASTHLG KDLVAAKNLL NRHEVILADI ASHEPRIQVI 850
    TERGNKMVEE GHFAAEDIAS RVESLNKNME SLHARAIRRE NDLKANVQLQ 900
    QYLADLHEAE AWIKEKEPIV DNKNYGADEE AAGALLKKHE AFLVDLNAFE 950
    NSIKALRDQA EVCQQQQAAP VDEAGREARV IALYDFEARS RREVSMKKND 1000
    VLTLLSSINK DWWKVEADDH QGFVPAVYVR KLAPDELPGF PQHRQEEPVN 1050
    IPQLQQQVET LYHSLLDRAE ERRRRLLQRY NEFLLAYEAG DMLEWIQEKK 1100
    TENTGVELDD VWELQKKFDE FQRDLKSNEP RLKDINKVAD ELLFEELLTP 1150
    EGAHIRQELN TRWNSLKRLA DEQYQLLSSA HAVEMFHREA DDVKEQIDKK 1200
    CRALNAADPG SDLLSVQALQ RQHEVFERDI IPLGEKVTTL GETAERLCES 1250
    HPDATEDLQK QRTELNEAWD TLQGLTSDRK ESLNEAHKFF LFLSKASDLE 1300
    NWIKTIGGVI SSPELAEDLT GTEILLERHQ EHHDDIKRED PTFQALEDFG 1350
    TELIDSGHRN RREIDNTLQN INSKRDNLEK SWENRKKMLD QCLELQLFRG 1400
    KCDQVESWMV ARENSLRSDD RDHLNSLQAL MKKRDDLDKA ITAQEGKISD 1450
    LENVATRLID NDHYAKEEIA ARLQRVLDRW KALKEQLLTE LGKLGDYADL 1500
    KQFYRDLEDL EEWINEMLPI ACDESYKDPT NIQRKYLKHQ AFENEVNGRA 1550
    EQVDGVINLG NSLIERRVCD GDEENMQEQL DKLKENWDYL LERTTDKGQK 1600
    LNEASRQQRF NTSIRDFEFW LSEAEGLLAM KDQARDLTSA GNLLKKHQLL 1650
    EAEMLAREDP LKDLNDLAQE LISSGTFNID QIEEKMNGVN ERFENVQSLA 1700
    AAHHEKLKET YALFQFFQDL DDEEAWIEEK LLRVSSQDYG RDLQSVQNLL 1750
    KKHKRLEGEL VAHEPAVQNV LDTAESLRDK AAVGKEEIQE RLAQFVQHWE 1800
    KLKELAKTRG VNLEESLEYL QFMENAEEEE AWLGEKCALV SRGDSGDTLA 1850
    ATQSLLKKHE ALENDFAVHK NRVQDVCAQG EDILNKEETQ NKDKISTKIQ 1900
    VLNEKTASLA KALAAWKSQL DDVHAFQQFN WKADVVESWI GEKEASLKTK 1950
    SNGADLTAFL TLLAKHDTLD ASLQSFQQER LSEIAELKDQ LVAGEHSQAK 2000
    AIEEQHAALL RHWEQLLEAS RVHRQKLLEK QLPLQKAEEL FMEFAHKASA 2050
    FNNWCENAEE DLSEPVHCVS LNEIRQLQKE HEAFLASLAG AQEDFNYLLE 2100
    LDKQIKALNV PSSPYTWLTV DVLGRIWNHL PDIIKEREQE LQKEEARQIK 2150
    NFEMCQEFEQ NASAFLQWIQ ETRAYFLDGS LLKETGTLES QLEANKRKQK 2200
    EIQAMKRHLT KIEDLGDSME EALILDIKYS TIGLAQQWDQ LHQLGMRMQH 2250
    NLEQQIQAKD TIGVSEETLK EFSTTYKHFD ENLTGRLTHK EFRSCLRGLN 2300
    YYLPMVEEGE PEPKFEKFLN AVDPGRKGYV SLEDYTSFLI DKESENIKTS 2350
    DDIESAFQAL AEGKAYITKE DMKQALTPEQ VSFCTIHMQQ YMDPRGRSQP 2400
    AGYDYVGFTN SFFGN 2415
    Length:2,415
    Mass (Da):279,865
    Last modified:July 7, 2009 - v3
    Checksum:i510B61CDEC4FAD13
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371P → L in AAB47540. 1 PublicationCurated
    Sequence conflicti501 – 5011D → Y in AAI50748. (PubMed:15489334)Curated
    Sequence conflicti716 – 7161G → V in AAB47540. 1 PublicationCurated
    Sequence conflicti741 – 7411V → M in AAB47540. 1 PublicationCurated
    Sequence conflicti741 – 7411V → M in AAI50748. (PubMed:15489334)Curated
    Sequence conflicti1044 – 10452RQ → QR in AAB47540. 1 PublicationCurated
    Sequence conflicti1044 – 10452RQ → QR in AAI50748. (PubMed:15489334)Curated
    Sequence conflicti1225 – 12251V → C in AAB47540. 1 PublicationCurated
    Sequence conflicti1238 – 12381T → N in AAB47540. 1 PublicationCurated
    Sequence conflicti1425 – 14251N → D in AAB47540. 1 PublicationCurated
    Sequence conflicti1582 – 15821K → M in AAB47540. 1 PublicationCurated
    Sequence conflicti2062 – 20621L → M in AAB47540. 1 PublicationCurated
    Sequence conflicti2062 – 20621L → M in AAI50748. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87455 mRNA. Translation: AAB47540.1.
    AF093576 mRNA. Translation: AAC61874.1.
    AC113483 mRNA. No translation available.
    AC156549 mRNA. No translation available.
    BC150747 mRNA. Translation: AAI50748.1.
    M10276 mRNA. Translation: AAA40123.1.
    CCDSiCCDS35795.1.
    PIRiA05283.
    RefSeqiNP_035595.2. NM_011465.4.
    UniGeneiMm.200611.

    Genome annotation databases

    EnsembliENSMUST00000027817; ENSMUSP00000027817; ENSMUSG00000026532.
    GeneIDi20739.
    KEGGimmu:20739.
    UCSCiuc007dsw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87455 mRNA. Translation: AAB47540.1 .
    AF093576 mRNA. Translation: AAC61874.1 .
    AC113483 mRNA. No translation available.
    AC156549 mRNA. No translation available.
    BC150747 mRNA. Translation: AAI50748.1 .
    M10276 mRNA. Translation: AAA40123.1 .
    CCDSi CCDS35795.1.
    PIRi A05283.
    RefSeqi NP_035595.2. NM_011465.4.
    UniGenei Mm.200611.

    3D structure databases

    ProteinModelPortali P08032.
    SMRi P08032. Positions 4-953, 992-1032, 1047-2260, 2263-2411.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203458. 5 interactions.
    IntActi P08032. 2 interactions.
    MINTi MINT-85326.

    PTM databases

    PhosphoSitei P08032.

    Proteomic databases

    MaxQBi P08032.
    PaxDbi P08032.
    PRIDEi P08032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027817 ; ENSMUSP00000027817 ; ENSMUSG00000026532 .
    GeneIDi 20739.
    KEGGi mmu:20739.
    UCSCi uc007dsw.2. mouse.

    Organism-specific databases

    CTDi 6708.
    MGIi MGI:98385. Spta1.

    Phylogenomic databases

    eggNOGi NOG237318.
    GeneTreei ENSGT00750000117361.
    HOGENOMi HOG000246965.
    HOVERGENi HBG059266.
    InParanoidi B2RWX6.
    KOi K06114.
    OMAi SINKDWW.
    OrthoDBi EOG7GXP9K.
    PhylomeDBi P08032.
    TreeFami TF343803.

    Miscellaneous databases

    NextBioi 299373.
    PROi P08032.
    SOURCEi Search...

    Gene expression databases

    Bgeei P08032.
    CleanExi MM_SPNA1.
    Genevestigatori P08032.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR002048. EF_hand_dom.
    IPR001452. SH3_domain.
    IPR018159. Spectrin/alpha-actinin.
    IPR013315. Spectrin_alpha_SH3.
    IPR002017. Spectrin_repeat.
    [Graphical view ]
    Pfami PF08726. EFhand_Ca_insen. 1 hit.
    PF00018. SH3_1. 1 hit.
    PF00435. Spectrin. 20 hits.
    [Graphical view ]
    PRINTSi PR01887. SPECTRNALPHA.
    SMARTi SM00326. SH3. 1 hit.
    SM00150. SPEC. 20 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50222. EF_HAND_2. 3 hits.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Burnett R.C., Avery A.C., Swardson C.J.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/cJ.
    2. Birkenmeier C.S., Gifford E.J., Barker J.E.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Sequence comparison of human and murine erythrocyte alpha-spectrin cDNA."
      Curtis P.J., Palumbo A., Ming J., Fraser P.J., Cioe L., Meo P., Shane S., Rovera G.
      Gene 36:357-362(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1567-1819.

    Entry informationi

    Entry nameiSPTA1_MOUSE
    AccessioniPrimary (citable) accession number: P08032
    Secondary accession number(s): B2RWX6, P97502
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This complex is anchored to the cytoplasmic face of the plasma membrane via another protein, ankyrin, which binds to beta-spectrin and mediates the binding of the whole complex to a transmembrane protein band 3. The interaction of erythrocyte spectrin with other proteins through specific binding domains lead to the formation of an extensive subplasmalemmal meshwork which is thought to be responsible for the maintenance of the biconcave shape of human erythrocytes, for the regulation of plasma membrane components and for the maintenance of the lipid asymmetry of the plasma membrane.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3