P08030 (APT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine phosphoribosyltransferase
Short name=APRT
EC=2.4.2.7

Gene names

Name:

Aprt

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP-Rule MF_00004
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00004
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP-Rule MF_00004
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00004.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	AMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway adenine metabolic process Inferred from mutant phenotype PubMed 8643571PubMed 8894695. Source: MGI adenine salvage Inferred from mutant phenotype PubMed 8864750. Source: MGI cellular response to insulin stimulus Inferred from electronic annotation. Source: Ensembl grooming behavior Inferred from genetic interaction PubMed 8485579. Source: MGI lactation Inferred from electronic annotation. Source: Ensembl purine ribonucleoside salvage Inferred from mutant phenotype PubMed 8485579. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: Ensembl
Molecular_function	AMP binding Inferred from electronic annotation. Source: Ensembl adenine binding Inferred from direct assay PubMed 9218001. Source: MGI adenine phosphoribosyltransferase activity Inferred from direct assay PubMed 1235912PubMed 198184PubMed 6307822PubMed 7323947PubMed 7444718. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 180	179	Adenine phosphoribosyltransferase HAMAP-Rule MF_00004		PRO_0000149506
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	15	1	Phosphoserine Ref.8
	Modified residue	60	1	Phosphotyrosine By similarity
	Modified residue	66	1	Phosphoserine By similarity
	Modified residue	114	1	N6-acetyllysine By similarity
	Modified residue	135	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	6 – 7	2	LK → FE in BAB22029. Ref.3
	Sequence conflict	15	1	S → V in AAA37255. Ref.1
	Sequence conflict	24	1	V → A in BAB22029. Ref.3
	Sequence conflict	46	1	L → S in BAB22029. Ref.3
	Sequence conflict	72	1	G → A in BAB22029. Ref.3
	Sequence conflict	107	1	K → KVR in AAA37256. Ref.7
	Sequence conflict	135	1	T → I in BAD95572. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08030 [UniParc]. Last modified February 6, 2013. Version 2. Checksum: A6AE4DBE0B456E42	FASTA	180	19,724
	10 20 30 40 50 60 MSEPELKLVA RRIRSFPDFP IPGVLFRDIS PLLKDPDSFR ASIRLLASHL KSTHSGKIDY 70 80 90 100 110 120 IAGLDSRGFL FGPSLAQELG VGCVLIRKQG KLPGPTVSAS YSLEYGKAEL EIQKDALEPG 130 140 150 160 170 180 QRVVIVDDLL ATGGTMFAAC DLLHQLRAEV VECVSLVELT SLKGRERLGP IPFFSLLQYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement." Dush M.K., Sikela J.M., Khan S.A., Tischfield J.A., Stambrook P.J. Proc. Natl. Acad. Sci. U.S.A. 82:2731-2735(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"mRNA aprt (wild-type allele) in murine SR-1 cell line." Fujimori A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Carcinoma.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow and Kidney.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Mammary tumor.
[6]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"Region-specific rates of molecular evolution: a fourfold reduction in the rate of accumulation of 'silent' mutations in transcribed versus nontranscribed regions of homologous DNA fragments derived from two closely related mouse species." Turker M.S., Cooper G.E., Bishop P.L. J. Mol. Evol. 36:31-40(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 63-133.
[8]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11310 mRNA. Translation: AAA37255.1. AB033539 mRNA. Translation: BAD95571.1. AB033540 mRNA. Translation: BAD95572.1. AK002350 mRNA. Translation: BAB22029.1. AK153201 mRNA. Translation: BAE31801.1. AC114917 Genomic DNA. No translation available. CH466525 Genomic DNA. Translation: EDL11692.1. BC005667 mRNA. Translation: AAH05667.1. M86439 Genomic DNA. Translation: AAA37256.1.
PIR	RTMSA. A22670.
RefSeq	NP_033828.2. NM_009698.2.
UniGene	Mm.1786.
3D structure databases
ProteinModelPortal	P08030.
SMR	P08030. Positions 2-180.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
IntAct	P08030. 4 interactions.
MINT	MINT-4088065.
STRING	10090.ENSMUSP00000006764.
PTM databases
PhosphoSite	P08030.
Proteomic databases
PaxDb	P08030.
PRIDE	P08030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006764; ENSMUSP00000006764; ENSMUSG00000006589.
GeneID	11821.
KEGG	mmu:11821.
UCSC	uc009ntf.1. mouse.
Organism-specific databases
CTD	353.
MGI	MGI:88061. Aprt.
Phylogenomic databases
eggNOG	COG0503.
GeneTree	ENSGT00390000017259.
HOGENOM	HOG000036776.
HOVERGEN	HBG003144.
InParanoid	Q61319.
KO	K00759.
OMA	VHALCAF.
OrthoDB	EOG7FFMT9.
TreeFam	TF300227.
Enzyme and pathway databases
UniPathway	UPA00588; UER00646.
Gene expression databases
CleanEx	MM_APRT.
Genevestigator	P08030.
Family and domain databases
HAMAP	MF_00004. Aden_phosphoribosyltr.
InterPro	IPR005764. Ade_phspho_trans. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01090. apt. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	APRT. mouse.
NextBio	279721.
PRO	P08030.
SOURCE	Search...

Entry information

Entry name

APT_MOUSE

Accession

Primary (citable) accession number: P08030
Secondary accession number(s): Q564P4 , Q61319, Q6PK77, Q9DCY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 6, 2013
Last modified:	April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program