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Protein

Adenine phosphoribosyltransferase

Gene

Aprt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase (Aprt)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

  • adenine binding Source: MGI
  • adenine phosphoribosyltransferase activity Source: MGI
  • AMP binding Source: MGI

GO - Biological processi

  • adenine metabolic process Source: MGI
  • adenine salvage Source: MGI
  • AMP salvage Source: UniProtKB-UniPathway
  • cellular response to insulin stimulus Source: Ensembl
  • grooming behavior Source: MGI
  • lactation Source: Ensembl
  • purine ribonucleoside salvage Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-74217 Purine salvage
UniPathwayiUPA00588; UER00646

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase (EC:2.4.2.7)
Short name:
APRT
Gene namesi
Name:Aprt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:88061 Aprt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001495062 – 180Adenine phosphoribosyltransferaseAdd BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei30PhosphoserineBy similarity1
Modified residuei60PhosphotyrosineBy similarity1
Modified residuei66PhosphoserineBy similarity1
Modified residuei114N6-acetyllysineBy similarity1
Modified residuei135PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP08030
PaxDbiP08030
PeptideAtlasiP08030
PRIDEiP08030

PTM databases

iPTMnetiP08030
PhosphoSitePlusiP08030

Expressioni

Gene expression databases

BgeeiENSMUSG00000006589
CleanExiMM_APRT
ExpressionAtlasiP08030 baseline and differential
GenevisibleiP08030 MM

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi1981683 interactors.
IntActiP08030 4 interactors.
MINTiP08030
STRINGi10090.ENSMUSP00000006764

Structurei

3D structure databases

ProteinModelPortaliP08030
SMRiP08030
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1712 Eukaryota
COG0503 LUCA
GeneTreeiENSGT00390000017259
HOGENOMiHOG000036776
HOVERGENiHBG003144
InParanoidiP08030
KOiK00759
OMAiCAPIRKK
OrthoDBiEOG091G0XZC
TreeFamiTF300227

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_00004 Aden_phosphoribosyltr, 1 hit
InterProiView protein in InterPro
IPR005764 Ade_phspho_trans
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01090 apt, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPELKLVA RRIRSFPDFP IPGVLFRDIS PLLKDPDSFR ASIRLLASHL
60 70 80 90 100
KSTHSGKIDY IAGLDSRGFL FGPSLAQELG VGCVLIRKQG KLPGPTVSAS
110 120 130 140 150
YSLEYGKAEL EIQKDALEPG QRVVIVDDLL ATGGTMFAAC DLLHQLRAEV
160 170 180
VECVSLVELT SLKGRERLGP IPFFSLLQYD
Length:180
Mass (Da):19,724
Last modified:February 6, 2013 - v2
Checksum:iA6AE4DBE0B456E42
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 7LK → FE in BAB22029 (PubMed:16141072).Curated2
Sequence conflicti15S → V in AAA37255 (PubMed:3921964).Curated1
Sequence conflicti24V → A in BAB22029 (PubMed:16141072).Curated1
Sequence conflicti46L → S in BAB22029 (PubMed:16141072).Curated1
Sequence conflicti72G → A in BAB22029 (PubMed:16141072).Curated1
Sequence conflicti107K → KVR in AAA37256 (PubMed:8433377).Curated1
Sequence conflicti135T → I in BAD95572 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11310 mRNA Translation: AAA37255.1
AB033539 mRNA Translation: BAD95571.1
AB033540 mRNA Translation: BAD95572.1
AK002350 mRNA Translation: BAB22029.1
AK153201 mRNA Translation: BAE31801.1
AC114917 Genomic DNA No translation available.
CH466525 Genomic DNA Translation: EDL11692.1
BC005667 mRNA Translation: AAH05667.1
M86439 Genomic DNA Translation: AAA37256.1
CCDSiCCDS40503.1
PIRiA22670 RTMSA
RefSeqiNP_033828.2, NM_009698.2
UniGeneiMm.1786

Genome annotation databases

EnsembliENSMUST00000006764; ENSMUSP00000006764; ENSMUSG00000006589
GeneIDi11821
KEGGimmu:11821
UCSCiuc009ntf.1 mouse

Similar proteinsi

Entry informationi

Entry nameiAPT_MOUSE
AccessioniPrimary (citable) accession number: P08030
Secondary accession number(s): Q564P4
, Q61319, Q6PK77, Q9DCY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 6, 2013
Last modified: February 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome