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Protein

Adenine phosphoribosyltransferase

Gene

Aprt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase (Aprt)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

  • adenine binding Source: MGI
  • adenine phosphoribosyltransferase activity Source: MGI
  • AMP binding Source: MGI

GO - Biological processi

  • adenine metabolic process Source: MGI
  • adenine salvage Source: MGI
  • AMP salvage Source: UniProtKB-UniPathway
  • cellular response to insulin stimulus Source: Ensembl
  • grooming behavior Source: MGI
  • lactation Source: Ensembl
  • purine ribonucleoside salvage Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-74217. Purine salvage.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase (EC:2.4.2.7)
Short name:
APRT
Gene namesi
Name:Aprt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:88061. Aprt.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001495062 – 180Adenine phosphoribosyltransferaseAdd BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei30PhosphoserineBy similarity1
Modified residuei60PhosphotyrosineBy similarity1
Modified residuei66PhosphoserineBy similarity1
Modified residuei114N6-acetyllysineBy similarity1
Modified residuei135PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP08030.
PaxDbiP08030.
PeptideAtlasiP08030.
PRIDEiP08030.

PTM databases

iPTMnetiP08030.
PhosphoSitePlusiP08030.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006589.
CleanExiMM_APRT.
GenevisibleiP08030. MM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi198168. 3 interactors.
IntActiP08030. 4 interactors.
MINTiMINT-4088065.
STRINGi10090.ENSMUSP00000006764.

Structurei

3D structure databases

ProteinModelPortaliP08030.
SMRiP08030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1712. Eukaryota.
COG0503. LUCA.
GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
HOVERGENiHBG003144.
InParanoidiP08030.
KOiK00759.
OMAiATVEIHA.
OrthoDBiEOG091G0XZC.
TreeFamiTF300227.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr. 1 hit.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPELKLVA RRIRSFPDFP IPGVLFRDIS PLLKDPDSFR ASIRLLASHL
60 70 80 90 100
KSTHSGKIDY IAGLDSRGFL FGPSLAQELG VGCVLIRKQG KLPGPTVSAS
110 120 130 140 150
YSLEYGKAEL EIQKDALEPG QRVVIVDDLL ATGGTMFAAC DLLHQLRAEV
160 170 180
VECVSLVELT SLKGRERLGP IPFFSLLQYD
Length:180
Mass (Da):19,724
Last modified:February 6, 2013 - v2
Checksum:iA6AE4DBE0B456E42
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 7LK → FE in BAB22029 (PubMed:16141072).Curated2
Sequence conflicti15S → V in AAA37255 (PubMed:3921964).Curated1
Sequence conflicti24V → A in BAB22029 (PubMed:16141072).Curated1
Sequence conflicti46L → S in BAB22029 (PubMed:16141072).Curated1
Sequence conflicti72G → A in BAB22029 (PubMed:16141072).Curated1
Sequence conflicti107K → KVR in AAA37256 (PubMed:8433377).Curated1
Sequence conflicti135T → I in BAD95572 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11310 mRNA. Translation: AAA37255.1.
AB033539 mRNA. Translation: BAD95571.1.
AB033540 mRNA. Translation: BAD95572.1.
AK002350 mRNA. Translation: BAB22029.1.
AK153201 mRNA. Translation: BAE31801.1.
AC114917 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11692.1.
BC005667 mRNA. Translation: AAH05667.1.
M86439 Genomic DNA. Translation: AAA37256.1.
CCDSiCCDS40503.1.
PIRiA22670. RTMSA.
RefSeqiNP_033828.2. NM_009698.2.
UniGeneiMm.1786.

Genome annotation databases

EnsembliENSMUST00000006764; ENSMUSP00000006764; ENSMUSG00000006589.
GeneIDi11821.
KEGGimmu:11821.
UCSCiuc009ntf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11310 mRNA. Translation: AAA37255.1.
AB033539 mRNA. Translation: BAD95571.1.
AB033540 mRNA. Translation: BAD95572.1.
AK002350 mRNA. Translation: BAB22029.1.
AK153201 mRNA. Translation: BAE31801.1.
AC114917 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11692.1.
BC005667 mRNA. Translation: AAH05667.1.
M86439 Genomic DNA. Translation: AAA37256.1.
CCDSiCCDS40503.1.
PIRiA22670. RTMSA.
RefSeqiNP_033828.2. NM_009698.2.
UniGeneiMm.1786.

3D structure databases

ProteinModelPortaliP08030.
SMRiP08030.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198168. 3 interactors.
IntActiP08030. 4 interactors.
MINTiMINT-4088065.
STRINGi10090.ENSMUSP00000006764.

PTM databases

iPTMnetiP08030.
PhosphoSitePlusiP08030.

Proteomic databases

EPDiP08030.
PaxDbiP08030.
PeptideAtlasiP08030.
PRIDEiP08030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006764; ENSMUSP00000006764; ENSMUSG00000006589.
GeneIDi11821.
KEGGimmu:11821.
UCSCiuc009ntf.1. mouse.

Organism-specific databases

CTDi353.
MGIiMGI:88061. Aprt.

Phylogenomic databases

eggNOGiKOG1712. Eukaryota.
COG0503. LUCA.
GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
HOVERGENiHBG003144.
InParanoidiP08030.
KOiK00759.
OMAiATVEIHA.
OrthoDBiEOG091G0XZC.
TreeFamiTF300227.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00646.
ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-74217. Purine salvage.

Miscellaneous databases

ChiTaRSiAprt. mouse.
PROiP08030.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006589.
CleanExiMM_APRT.
GenevisibleiP08030. MM.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr. 1 hit.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPT_MOUSE
AccessioniPrimary (citable) accession number: P08030
Secondary accession number(s): Q564P4
, Q61319, Q6PK77, Q9DCY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 6, 2013
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.