P08026 (STXA_BPH19) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shiga-like toxin 1 subunit A
Short name=SLT-1 A subunit
Short name=SLT-1a
Short name=SLT-Ia
EC=3.2.2.22

Alternative name(s):
Verocytotoxin 1 subunit A
Verotoxin 1 subunit A
rRNA N-glycosidase 1

Gene names

Name:	stxA
Synonyms:	sltA

Organism

Enterobacteria phage H19B (Bacteriophage H19B)

Taxonomic identifier

69932 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses › unclassified Lambda-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subnit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Shiga-like toxin contains a single subunit A and five copies of subunit B.
Subcellular location	Secreted.
Sequence similarities	Belongs to the ribosome-inactivating protein family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 315	293	Shiga-like toxin 1 subunit A		PRO_0000030791
Regions
	Region	23 – 273	251	A1 By similarity
	Region	274 – 315	42	A2 By similarity
Sites
	Active site	189	1	Ref.3
	Site	273 – 274	2	Cleavage; by furin By similarity
Amino acid modifications
	Disulfide bond	264 ↔ 283		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08026 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 8B993DF7A8E58F30	FASTA	315	34,800
	10 20 30 40 50 60 MKIIIFRVLT FFFVIFSVNV VAKEFTLDFS TAKTYVDSLN VIRSAIGTPL QTISSGGTSL 70 80 90 100 110 120 LMIDSGSGDN LFAVDVRGID PEEGRFNNLR LIVERNNLYV TGFVNRTNNV FYRFADFSHV 130 140 150 160 170 180 TFPGTTAVTL SGDSSYTTLQ RVAGISRTGM QINRHSLTTS YLDLMSHSGT SLTQSVARAM 190 200 210 220 230 240 LRFVTVTAEA LRFRQIQRGF RTTLDDLSGR SYVMTAEDVD LTLNWGRLSS VLPDYHGQDS 250 260 270 280 290 300 VRVGRISFGS INAILGSVAL ILNCHHHASR VARMASDEFP SMCPADGRVR GITHNKILWD 310 SSTLGAILMR RTISS

References

[1]	"Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli." Calderwood S.B., Auclair F., Donohue-Rolfe A., Keusch G.T., Mekalanos J.J. Proc. Natl. Acad. Sci. U.S.A. 84:4364-4368(1987) [PubMed: 3299365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence and promoter mapping of the Escherichia coli Shiga-like toxin operon of bacteriophage H-19B." de Grandis S., Ginsberg J., Toone M., Climie S., Friesen J., Brunton J.L. J. Bacteriol. 169:4313-4319(1987) [PubMed: 3040689] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I." Hovde C.J., Calderwood S.B., Mekalanos J.J., Collier R.J. Proc. Natl. Acad. Sci. U.S.A. 85:2568-2572(1988) [PubMed: 3357883] [Abstract] Cited for: ACTIVE SITE.
[4]	"Evidence that the A2 fragment of Shiga-like toxin type I is required for holotoxin integrity." Austin P.R., Jablonski P.E., Bohach G.A., Dunker A.K., Hovde C.J. Infect. Immun. 62:1768-1775(1994) [PubMed: 8168939] [Abstract] Cited for: ROLE OF A2 FRAGMENT IN HOLOTOXIN ASSEMBLY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16625 Genomic DNA. Translation: AAA98099.1. M17358 Genomic DNA. Translation: AAA32229.1.
PIR	XUBPH9. A27052. A53887.
3D structure databases
ProteinModelPortal	P08026.
SMR	P08026. Positions 23-312.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR016331. Shiga-like_toxin_subunit_A. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PIRSF	PIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAM	SSF56371. Ribosome_inactivat_prot. 1 hit.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STXA_BPH19

Accession

Primary (citable) accession number: P08026

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	May 31, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program