P08019 (AMYG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucoamylase, intracellular sporulation-specific
EC=3.2.1.3

Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase

Gene names

Name:	SGA1
Synonyms:	SGA
Ordered Locus Names:	YIL099W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 15 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation Sporulation
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ascospore formation Inferred from expression pattern PubMed 350852. Source: SGD glycogen catabolic process Inferred from mutant phenotype PubMed 11486014. Source: SGD
Cellular_component	fungal-type vacuole Inferred from direct assay PubMed 2493265. Source: SGD
Molecular_function	glucan 1,4-alpha-glucosidase activity Inferred from direct assay PubMed 2493265. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Glucoamylase, intracellular sporulation-specific		PRO_0000186120
Sites
	Active site	261	1	Proton acceptor By similarity
	Active site	264	1	Proton donor By similarity
	Binding site	198	1	Substrate By similarity
Experimental info
	Sequence conflict	184	1	D → H in CAA32071. Ref.4
	Sequence conflict	504 – 549	46	HVGTD…VLQSL → TWEQTGN in AAA35042. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08019 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 6351E84F2CF4AB77	FASTA	549	61,463
	10 20 30 40 50 60 MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN NGPSQVQLRD 70 80 90 100 110 120 AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI GPSAVYPSIS PGVVIASPSQ 130 140 150 160 170 180 THPDYFYQWI RDSALTINSI VSHSAGPAIE TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV 190 200 210 220 230 240 ALGDPKWNVD NTAFTEDWGR PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF 250 260 270 280 290 300 DDIVRWDLRF IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE 310 320 330 340 350 360 LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN TVHDAPSASH 370 380 390 400 410 420 LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG RYPEDVYDGY GFGEGNPWVL 430 440 450 460 470 480 ATCTASTTLY QLIYRHISEQ HDLVVPMNND CSNAFWSELV FSNLTTLGND EGYLILEFNT 490 500 510 520 530 540 PAFNQTIQKI FQLADSFLVK LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ IRQEVLQSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene fusion is a possible mechanism underlying the evolution of STA1." Yamashita I., Nakamura M., Fukui S. J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae." Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A. FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190. Strain: SPX101-1C.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38125 Genomic DNA. Translation: CAA86282.1. M16166 Genomic DNA. Translation: AAA35042.1. X13858 Genomic DNA. Translation: CAA32071.1. BK006942 Genomic DNA. Translation: DAA08454.1.
PIR	S48474.
RefSeq	NP_012167.3. NM_001179447.3.
3D structure databases
ProteinModelPortal	P08019.
SMR	P08019. Positions 80-546.
ModBase	Search...
Protein-protein interaction databases
IntAct	P08019. 1 interaction.
MINT	MINT-2784002.
Protein family/group databases
CAZy	GH15. Glycoside Hydrolase Family 15.
Proteomic databases
PRIDE	P08019.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL099W; YIL099W; YIL099W.
GeneID	854708.
KEGG	sce:YIL093C. sce:YIL099W.
Organism-specific databases
CYGD	YIL099w.
SGD	S000001361. SGA1.
Phylogenomic databases
HOGENOM	HOG000182646.
KO	K01178.
OMA	IGNPWFI.
OrthoDB	EOG4FFH90.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13682.
Gene expression databases
Genevestigator	P08019.
GermOnline	YIL099W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR000165. Glucoamylase. IPR011613. Glyco_hydro_15. [Graphical view]
Pfam	PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
PRINTS	PR00736. GLHYDRLASE15.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00820. GLUCOAMYLASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075248.
NextBio	977366.

Entry information

Entry name

AMYG_YEAST

Accession

Primary (citable) accession number: P08019
Secondary accession number(s): D6VVI8, Q07070

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program