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Protein

Glucoamylase, intracellular sporulation-specific

Gene

SGA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei198 – 1981SubstrateBy similarity
Active sitei261 – 2611Proton acceptorPROSITE-ProRule annotation
Active sitei264 – 2641Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: SGD

GO - Biological processi

  1. glycogen catabolic process Source: SGD
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13682.
YEAST:YIL099W-MONOMER.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase, intracellular sporulation-specific (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:SGA1
Synonyms:SGA
Ordered Locus Names:YIL099W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIL099w.
SGDiS000001361. SGA1.

Subcellular locationi

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Glucoamylase, intracellular sporulation-specificPRO_0000186120Add
BLAST

Proteomic databases

PRIDEiP08019.

Expressioni

Gene expression databases

GenevestigatoriP08019.

Interactioni

Protein-protein interaction databases

BioGridi34892. 10 interactions.
IntActiP08019. 1 interaction.
MINTiMINT-2784002.

Structurei

3D structure databases

ProteinModelPortaliP08019.
SMRiP08019. Positions 80-546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Phylogenomic databases

HOGENOMiHOG000182646.
InParanoidiP08019.
KOiK01178.
OMAiLEYVSHY.
OrthoDBiEOG7C2R8Z.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN
60 70 80 90 100
NGPSQVQLRD AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI
110 120 130 140 150
GPSAVYPSIS PGVVIASPSQ THPDYFYQWI RDSALTINSI VSHSAGPAIE
160 170 180 190 200
TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV ALGDPKWNVD NTAFTEDWGR
210 220 230 240 250
PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF DDIVRWDLRF
260 270 280 290 300
IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE
310 320 330 340 350
LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN
360 370 380 390 400
TVHDAPSASH LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG
410 420 430 440 450
RYPEDVYDGY GFGEGNPWVL ATCTASTTLY QLIYRHISEQ HDLVVPMNND
460 470 480 490 500
CSNAFWSELV FSNLTTLGND EGYLILEFNT PAFNQTIQKI FQLADSFLVK
510 520 530 540
LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ IRQEVLQSL
Length:549
Mass (Da):61,463
Last modified:February 1, 1995 - v2
Checksum:i6351E84F2CF4AB77
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841D → H in CAA32071 (PubMed:3141213).Curated
Sequence conflicti504 – 54946HVGTD…VLQSL → TWEQTGN in AAA35042 (PubMed:3106330).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86282.1.
M16166 Genomic DNA. Translation: AAA35042.1.
X13858 Genomic DNA. Translation: CAA32071.1.
BK006942 Genomic DNA. Translation: DAA08454.1.
PIRiS48474.
RefSeqiNP_012167.3. NM_001179447.3.

Genome annotation databases

EnsemblFungiiYIL099W; YIL099W; YIL099W.
GeneIDi854708.
KEGGisce:YIL099W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86282.1.
M16166 Genomic DNA. Translation: AAA35042.1.
X13858 Genomic DNA. Translation: CAA32071.1.
BK006942 Genomic DNA. Translation: DAA08454.1.
PIRiS48474.
RefSeqiNP_012167.3. NM_001179447.3.

3D structure databases

ProteinModelPortaliP08019.
SMRiP08019. Positions 80-546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34892. 10 interactions.
IntActiP08019. 1 interaction.
MINTiMINT-2784002.

Chemistry

BindingDBiP08019.
ChEMBLiCHEMBL1075248.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEiP08019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL099W; YIL099W; YIL099W.
GeneIDi854708.
KEGGisce:YIL099W.

Organism-specific databases

CYGDiYIL099w.
SGDiS000001361. SGA1.

Phylogenomic databases

HOGENOMiHOG000182646.
InParanoidiP08019.
KOiK01178.
OMAiLEYVSHY.
OrthoDBiEOG7C2R8Z.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13682.
YEAST:YIL099W-MONOMER.

Miscellaneous databases

NextBioi977366.

Gene expression databases

GenevestigatoriP08019.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene fusion is a possible mechanism underlying the evolution of STA1."
    Yamashita I., Nakamura M., Fukui S.
    J. Bacteriol. 169:2142-2149(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae."
    Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.
    FEBS Lett. 239:179-184(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190.
    Strain: SPX101-1C.

Entry informationi

Entry nameiAMYG_YEAST
AccessioniPrimary (citable) accession number: P08019
Secondary accession number(s): D6VVI8, Q07070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.