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P08019

- AMYG_YEAST

UniProt

P08019 - AMYG_YEAST

Protein

Glucoamylase, intracellular sporulation-specific

Gene

SGA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei198 – 1981SubstrateBy similarity
    Active sitei261 – 2611Proton acceptorPROSITE-ProRule annotation
    Active sitei264 – 2641Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. glycogen catabolic process Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Sporulation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13682.
    YEAST:YIL099W-MONOMER.

    Protein family/group databases

    CAZyiGH15. Glycoside Hydrolase Family 15.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase, intracellular sporulation-specific (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:SGA1
    Synonyms:SGA
    Ordered Locus Names:YIL099W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL099w.
    SGDiS000001361. SGA1.

    Subcellular locationi

    GO - Cellular componenti

    1. fungal-type vacuole Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 549549Glucoamylase, intracellular sporulation-specificPRO_0000186120Add
    BLAST

    Proteomic databases

    PRIDEiP08019.

    Expressioni

    Gene expression databases

    GenevestigatoriP08019.

    Interactioni

    Protein-protein interaction databases

    BioGridi34892. 10 interactions.
    IntActiP08019. 1 interaction.
    MINTiMINT-2784002.

    Structurei

    3D structure databases

    ProteinModelPortaliP08019.
    SMRiP08019. Positions 80-546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000182646.
    KOiK01178.
    OMAiLEYVSHY.
    OrthoDBiEOG7C2R8Z.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view]
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PRINTSiPR00736. GLHYDRLASE15.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08019-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN    50
    NGPSQVQLRD AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI 100
    GPSAVYPSIS PGVVIASPSQ THPDYFYQWI RDSALTINSI VSHSAGPAIE 150
    TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV ALGDPKWNVD NTAFTEDWGR 200
    PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF DDIVRWDLRF 250
    IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE 300
    LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN 350
    TVHDAPSASH LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG 400
    RYPEDVYDGY GFGEGNPWVL ATCTASTTLY QLIYRHISEQ HDLVVPMNND 450
    CSNAFWSELV FSNLTTLGND EGYLILEFNT PAFNQTIQKI FQLADSFLVK 500
    LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ IRQEVLQSL 549
    Length:549
    Mass (Da):61,463
    Last modified:February 1, 1995 - v2
    Checksum:i6351E84F2CF4AB77
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841D → H in CAA32071. (PubMed:3141213)Curated
    Sequence conflicti504 – 54946HVGTD…VLQSL → TWEQTGN in AAA35042. (PubMed:3106330)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38125 Genomic DNA. Translation: CAA86282.1.
    M16166 Genomic DNA. Translation: AAA35042.1.
    X13858 Genomic DNA. Translation: CAA32071.1.
    BK006942 Genomic DNA. Translation: DAA08454.1.
    PIRiS48474.
    RefSeqiNP_012167.3. NM_001179447.3.

    Genome annotation databases

    EnsemblFungiiYIL099W; YIL099W; YIL099W.
    GeneIDi854708.
    KEGGisce:YIL099W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38125 Genomic DNA. Translation: CAA86282.1 .
    M16166 Genomic DNA. Translation: AAA35042.1 .
    X13858 Genomic DNA. Translation: CAA32071.1 .
    BK006942 Genomic DNA. Translation: DAA08454.1 .
    PIRi S48474.
    RefSeqi NP_012167.3. NM_001179447.3.

    3D structure databases

    ProteinModelPortali P08019.
    SMRi P08019. Positions 80-546.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34892. 10 interactions.
    IntActi P08019. 1 interaction.
    MINTi MINT-2784002.

    Chemistry

    ChEMBLi CHEMBL1075248.

    Protein family/group databases

    CAZyi GH15. Glycoside Hydrolase Family 15.

    Proteomic databases

    PRIDEi P08019.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL099W ; YIL099W ; YIL099W .
    GeneIDi 854708.
    KEGGi sce:YIL099W.

    Organism-specific databases

    CYGDi YIL099w.
    SGDi S000001361. SGA1.

    Phylogenomic databases

    HOGENOMi HOG000182646.
    KOi K01178.
    OMAi LEYVSHY.
    OrthoDBi EOG7C2R8Z.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13682.
    YEAST:YIL099W-MONOMER.

    Miscellaneous databases

    NextBioi 977366.

    Gene expression databases

    Genevestigatori P08019.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view ]
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PRINTSi PR00736. GLHYDRLASE15.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene fusion is a possible mechanism underlying the evolution of STA1."
      Yamashita I., Nakamura M., Fukui S.
      J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae."
      Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.
      FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190.
      Strain: SPX101-1C.

    Entry informationi

    Entry nameiAMYG_YEAST
    AccessioniPrimary (citable) accession number: P08019
    Secondary accession number(s): D6VVI8, Q07070
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3