Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P08019 (AMYG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucoamylase, intracellular sporulation-specific

EC=3.2.1.3
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene names
Name:SGA1
Synonyms:SGA
Ordered Locus Names:YIL099W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Glucoamylase, intracellular sporulation-specific
PRO_0000186120

Sites

Active site2611Proton acceptor By similarity
Active site2641Proton donor By similarity
Binding site1981Substrate By similarity

Experimental info

Sequence conflict1841D → H in CAA32071. Ref.4
Sequence conflict504 – 54946HVGTD…VLQSL → TWEQTGN in AAA35042. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P08019 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 6351E84F2CF4AB77

FASTA54961,463
        10         20         30         40         50         60 
MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN NGPSQVQLRD 

        70         80         90        100        110        120 
AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI GPSAVYPSIS PGVVIASPSQ 

       130        140        150        160        170        180 
THPDYFYQWI RDSALTINSI VSHSAGPAIE TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV 

       190        200        210        220        230        240 
ALGDPKWNVD NTAFTEDWGR PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF 

       250        260        270        280        290        300 
DDIVRWDLRF IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE 

       310        320        330        340        350        360 
LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN TVHDAPSASH 

       370        380        390        400        410        420 
LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG RYPEDVYDGY GFGEGNPWVL 

       430        440        450        460        470        480 
ATCTASTTLY QLIYRHISEQ HDLVVPMNND CSNAFWSELV FSNLTTLGND EGYLILEFNT 

       490        500        510        520        530        540 
PAFNQTIQKI FQLADSFLVK LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ 


IRQEVLQSL 

« Hide

References

« Hide 'large scale' references
[1]"Gene fusion is a possible mechanism underlying the evolution of STA1."
Yamashita I., Nakamura M., Fukui S.
J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae."
Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.
FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190.
Strain: SPX101-1C.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38125 Genomic DNA. Translation: CAA86282.1.
M16166 Genomic DNA. Translation: AAA35042.1.
X13858 Genomic DNA. Translation: CAA32071.1.
BK006942 Genomic DNA. Translation: DAA08454.1.
PIRS48474.
RefSeqNP_012167.3. NM_001179447.3.

3D structure databases

ProteinModelPortalP08019.
SMRP08019. Positions 80-546.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34892. 10 interactions.
IntActP08019. 1 interaction.
MINTMINT-2784002.

Chemistry

ChEMBLCHEMBL1075248.

Protein family/group databases

CAZyGH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEP08019.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL099W; YIL099W; YIL099W.
GeneID854708.
KEGGsce:YIL099W.

Organism-specific databases

CYGDYIL099w.
SGDS000001361. SGA1.

Phylogenomic databases

HOGENOMHOG000182646.
KOK01178.
OMALEYVSHY.
OrthoDBEOG7C2R8Z.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13682.
YEAST:YIL099W-MONOMER.

Gene expression databases

GenevestigatorP08019.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSPR00736. GLHYDRLASE15.
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977366.

Entry information

Entry nameAMYG_YEAST
AccessionPrimary (citable) accession number: P08019
Secondary accession number(s): D6VVI8, Q07070
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries