SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P08019

- AMYG_YEAST

UniProt

P08019 - AMYG_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glucoamylase, intracellular sporulation-specific
Gene
SGA1, SGA, YIL099W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei198 – 1981Substrate By similarity
Active sitei261 – 2611Proton acceptor By similarity
Active sitei264 – 2641Proton donor By similarity

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: SGD

GO - Biological processi

  1. ascospore formation Source: SGD
  2. glycogen catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13682.
YEAST:YIL099W-MONOMER.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase, intracellular sporulation-specific (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:SGA1
Synonyms:SGA
Ordered Locus Names:YIL099W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL099w.
SGDiS000001361. SGA1.

Subcellular locationi

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Glucoamylase, intracellular sporulation-specific
PRO_0000186120Add
BLAST

Proteomic databases

PRIDEiP08019.

Expressioni

Gene expression databases

GenevestigatoriP08019.

Interactioni

Protein-protein interaction databases

BioGridi34892. 10 interactions.
IntActiP08019. 1 interaction.
MINTiMINT-2784002.

Structurei

3D structure databases

ProteinModelPortaliP08019.
SMRiP08019. Positions 80-546.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000182646.
KOiK01178.
OMAiLEYVSHY.
OrthoDBiEOG7C2R8Z.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08019-1 [UniParc]FASTAAdd to Basket

« Hide

MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN    50
NGPSQVQLRD AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI 100
GPSAVYPSIS PGVVIASPSQ THPDYFYQWI RDSALTINSI VSHSAGPAIE 150
TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV ALGDPKWNVD NTAFTEDWGR 200
PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF DDIVRWDLRF 250
IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE 300
LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN 350
TVHDAPSASH LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG 400
RYPEDVYDGY GFGEGNPWVL ATCTASTTLY QLIYRHISEQ HDLVVPMNND 450
CSNAFWSELV FSNLTTLGND EGYLILEFNT PAFNQTIQKI FQLADSFLVK 500
LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ IRQEVLQSL 549
Length:549
Mass (Da):61,463
Last modified:February 1, 1995 - v2
Checksum:i6351E84F2CF4AB77
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841D → H in CAA32071. 1 Publication
Sequence conflicti504 – 54946HVGTD…VLQSL → TWEQTGN in AAA35042. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38125 Genomic DNA. Translation: CAA86282.1.
M16166 Genomic DNA. Translation: AAA35042.1.
X13858 Genomic DNA. Translation: CAA32071.1.
BK006942 Genomic DNA. Translation: DAA08454.1.
PIRiS48474.
RefSeqiNP_012167.3. NM_001179447.3.

Genome annotation databases

EnsemblFungiiYIL099W; YIL099W; YIL099W.
GeneIDi854708.
KEGGisce:YIL099W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38125 Genomic DNA. Translation: CAA86282.1 .
M16166 Genomic DNA. Translation: AAA35042.1 .
X13858 Genomic DNA. Translation: CAA32071.1 .
BK006942 Genomic DNA. Translation: DAA08454.1 .
PIRi S48474.
RefSeqi NP_012167.3. NM_001179447.3.

3D structure databases

ProteinModelPortali P08019.
SMRi P08019. Positions 80-546.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34892. 10 interactions.
IntActi P08019. 1 interaction.
MINTi MINT-2784002.

Chemistry

ChEMBLi CHEMBL1075248.

Protein family/group databases

CAZyi GH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEi P08019.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL099W ; YIL099W ; YIL099W .
GeneIDi 854708.
KEGGi sce:YIL099W.

Organism-specific databases

CYGDi YIL099w.
SGDi S000001361. SGA1.

Phylogenomic databases

HOGENOMi HOG000182646.
KOi K01178.
OMAi LEYVSHY.
OrthoDBi EOG7C2R8Z.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13682.
YEAST:YIL099W-MONOMER.

Miscellaneous databases

NextBioi 977366.

Gene expression databases

Genevestigatori P08019.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view ]
Pfami PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
PRINTSi PR00736. GLHYDRLASE15.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene fusion is a possible mechanism underlying the evolution of STA1."
    Yamashita I., Nakamura M., Fukui S.
    J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae."
    Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.
    FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190.
    Strain: SPX101-1C.

Entry informationi

Entry nameiAMYG_YEAST
AccessioniPrimary (citable) accession number: P08019
Secondary accession number(s): D6VVI8, Q07070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi