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P08018

- PBS2_YEAST

UniProt

P08018 - PBS2_YEAST

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Protein

MAP kinase kinase PBS2

Gene

PBS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Seems to phosphorylate HOG1 on a tyrosine residue.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei389 – 3891ATPPROSITE-ProRule annotation
Active sitei485 – 4851Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi366 – 3749ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase activity Source: SGD
  3. MAP-kinase scaffold activity Source: SGD
  4. peptide alpha-N-acetyltransferase activity Source: UniProtKB
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. actin filament organization Source: SGD
  2. activation of MAPK activity involved in osmosensory signaling pathway Source: SGD
  3. cellular response to heat Source: SGD
  4. hyperosmotic response Source: SGD
  5. MAPK import into nucleus involved in osmosensory signaling pathway Source: SGD
  6. N-terminal peptidyl-methionine acetylation Source: UniProtKB
  7. osmosensory signaling pathway Source: UniProtKB
  8. protein phosphorylation Source: UniProtKB
  9. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31578-MONOMER.
BRENDAi2.7.12.2. 984.
ReactomeiREACT_188484. Oxidative Stress Induced Senescence.
REACT_189013. NOD1/2 Signaling Pathway.
REACT_191493. FCERI mediated MAPK activation.
REACT_259976. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_96987. activated TAK1 mediates p38 MAPK activation.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase kinase PBS2 (EC:2.7.12.2)
Alternative name(s):
Polymyxin B resistance protein 2
Suppressor of fluoride sensitivity 4
Gene namesi
Name:PBS2
Synonyms:HOG4, SFS4, SSK4
Ordered Locus Names:YJL128C
ORF Names:J0699
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL128c.
SGDiS000003664. PBS2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: SGD
  4. NatB complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961P → S in PBS2-13; loss of SH3-domain interaction. 1 Publication
Mutagenesisi389 – 3891K → M: Loss of activity. 1 Publication
Mutagenesisi514 – 5141S → A: Loss of phosphorylation. 1 Publication
Mutagenesisi518 – 5181T → A: Loss of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 668668MAP kinase kinase PBS2PRO_0000086483Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei68 – 681Phosphoserine1 Publication
Modified residuei269 – 2691Phosphoserine1 Publication
Modified residuei514 – 5141Phosphoserine2 Publications
Modified residuei518 – 5181Phosphothreonine1 Publication

Post-translational modificationi

Activated by phosphorylation by SSK2 or SSK22. Ser/Thr phosphorylation is also necessary for SHO1-mediated activation.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08018.
PaxDbiP08018.
PeptideAtlasiP08018.

Expressioni

Gene expression databases

GenevestigatoriP08018.

Interactioni

Subunit structurei

Interacts with NBP2, PTC1, SHO1 AND STE11.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NBP2Q121632EBI-12972,EBI-34713
SHO1P400735EBI-12972,EBI-18140

Protein-protein interaction databases

BioGridi33628. 289 interactions.
DIPiDIP-2368N.
IntActiP08018. 21 interactions.
MINTiMINT-546167.
STRINGi4932.YJL128C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VKNX-ray2.05C92-103[»]
ProteinModelPortaliP08018.
SMRiP08018. Positions 271-623.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08018.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini360 – 623264Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi91 – 10111Pro-richAdd
BLAST

Domaini

Alternative way of activation involves binding the proline-rich motif to the SH3 domain of SHO1.1 Publication

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
InParanoidiP08018.
KOiK11227.
OMAiTNILCSA.
OrthoDBiEOG7380F1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08018-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL
60 70 80 90 100
HARVKAFQEQ RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV
110 120 130 140 150
AGSSKVSQRM SSQVVQASSK STLKNVLDNQ ETQNITDVNI NIDTTKITAT
160 170 180 190 200
TIGVNTGLPA TDITPSVSNT ASATHKAQLL NPNRRAPRRP LSTQHPTRPN
210 220 230 240 250
VAPHKAPAII NTPKQSLSAR RGLKLPPGGM SLKMPTKTAQ QPQQFAPSPS
260 270 280 290 300
NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT
310 320 330 340 350
GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG
360 370 380 390 400
SSSRITLDEL EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF
410 420 430 440 450
RQILMELEVL HKCNSPYIVD FYGAFFIEGA VYMCMEYMDG GSLDKIYDES
460 470 480 490 500
SEIGGIDEPQ LAFIANAVIH GLKELKEQHN IIHRDVKPTN ILCSANQGTV
510 520 530 540 550
KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT YTVQSDIWSL
560 570 580 590 600
GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV
610 620 630 640 650
SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE
660
RGENGLSKNV PALHMGGL
Length:668
Mass (Da):72,720
Last modified:September 21, 2011 - v4
Checksum:i9BC3435BDAFE8019
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti222 – 2232GL → AV(PubMed:8948101)Curated
Sequence conflicti222 – 2232GL → AV in CAA89423. (PubMed:8641269)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02946 Unassigned DNA. Translation: AAA16819.1.
U12237 Genomic DNA. Translation: AAA20392.1.
Z49403 Genomic DNA. Translation: CAA89423.1.
BK006943 Genomic DNA. Translation: DAA08673.2.
PIRiS56909.
RefSeqiNP_012407.2. NM_001181561.2.

Genome annotation databases

EnsemblFungiiYJL128C; YJL128C; YJL128C.
GeneIDi853313.
KEGGisce:YJL128C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02946 Unassigned DNA. Translation: AAA16819.1 .
U12237 Genomic DNA. Translation: AAA20392.1 .
Z49403 Genomic DNA. Translation: CAA89423.1 .
BK006943 Genomic DNA. Translation: DAA08673.2 .
PIRi S56909.
RefSeqi NP_012407.2. NM_001181561.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VKN X-ray 2.05 C 92-103 [» ]
ProteinModelPortali P08018.
SMRi P08018. Positions 271-623.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33628. 289 interactions.
DIPi DIP-2368N.
IntActi P08018. 21 interactions.
MINTi MINT-546167.
STRINGi 4932.YJL128C.

Proteomic databases

MaxQBi P08018.
PaxDbi P08018.
PeptideAtlasi P08018.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL128C ; YJL128C ; YJL128C .
GeneIDi 853313.
KEGGi sce:YJL128C.

Organism-specific databases

CYGDi YJL128c.
SGDi S000003664. PBS2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119199.
HOGENOMi HOG000234206.
InParanoidi P08018.
KOi K11227.
OMAi TNILCSA.
OrthoDBi EOG7380F1.

Enzyme and pathway databases

BioCyci YEAST:G3O-31578-MONOMER.
BRENDAi 2.7.12.2. 984.
Reactomei REACT_188484. Oxidative Stress Induced Senescence.
REACT_189013. NOD1/2 Signaling Pathway.
REACT_191493. FCERI mediated MAPK activation.
REACT_259976. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_96987. activated TAK1 mediates p38 MAPK activation.

Miscellaneous databases

EvolutionaryTracei P08018.
NextBioi 973654.
PROi P08018.

Gene expression databases

Genevestigatori P08018.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases."
    Boguslawski G., Polazzi J.O.
    Proc. Natl. Acad. Sci. U.S.A. 84:5848-5852(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "PBS2, a yeast gene encoding a putative protein kinase, interacts with the RAS2 pathway and affects osmotic sensitivity of Saccharomyces cerevisiae."
    Boguslawski G.
    J. Gen. Microbiol. 138:2425-2432(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 222-223 AND 668.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon."
    Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C.
    Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 222-223.
    Strain: ATCC 204508 / S288c.
  7. "Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor."
    Maeda T., Takekawa M., Saito H.
    Science 269:554-558(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 91-101, DOMAIN, PHOSPHORYLATION AT SER-514 AND THR-518, MUTAGENESIS OF PRO-96; LYS-389; SER-514 AND THR-518.
  8. "An osmosensing signal transduction pathway in yeast."
    Brewster J.L., de Valoir T., Dwyer N.D., Winter E., Gustin M.C.
    Science 259:1760-1763(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway."
    Raitt D.C., Posas F., Saito H.
    EMBO J. 19:4623-4631(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SHO1.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway."
    Mapes J., Ota I.M.
    EMBO J. 23:302-311(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NBP2 AND PTC1.
  13. "A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast."
    Cullen P.J., Sabbagh W. Jr., Graham E., Irick M.M., van Olden E.K., Neal C., Delrow J., Bardwell L., Sprague G.F. Jr.
    Genes Dev. 18:1695-1708(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SHO1.
  14. "Protein-protein interaction affinity plays a crucial role in controlling the Sho1p-mediated signal transduction pathway in yeast."
    Marles J.A., Dahesh S., Haynes J., Andrews B.J., Davidson A.R.
    Mol. Cell 14:813-823(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHO1 AND STE11.
  15. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  16. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPBS2_YEAST
AccessioniPrimary (citable) accession number: P08018
Secondary accession number(s): D6VW57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 21, 2011
Last modified: November 26, 2014
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2160 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3