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P08011

- MGST1_RAT

UniProt

P08011 - MGST1_RAT

Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Enzyme regulationi

    Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide, except in the testis. Activation also occurs via nitration of Tyr-93 by peroxynitrite.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381Glutathione1 Publication
    Sitei50 – 501Activates the enzyme when modified
    Binding sitei73 – 731Glutathione1 Publication
    Binding sitei74 – 741Glutathione1 Publication
    Binding sitei76 – 761Glutathione1 Publication
    Binding sitei81 – 811Glutathione1 Publication
    Binding sitei121 – 1211Glutathione1 Publication

    GO - Molecular functioni

    1. glutathione binding Source: UniProtKB
    2. glutathione peroxidase activity Source: Ensembl
    3. glutathione transferase activity Source: UniProtKB
    4. identical protein binding Source: RGD
    5. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. cellular response to lipid hydroperoxide Source: Ensembl
    2. glutathione metabolic process Source: Ensembl
    3. Leydig cell differentiation Source: RGD
    4. protein homotrimerization Source: UniProtKB
    5. response to drug Source: RGD
    6. response to lipopolysaccharide Source: RGD
    7. response to organonitrogen compound Source: RGD

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    SABIO-RKP08011.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
    Short name:
    Microsomal GST-1
    Alternative name(s):
    Microsomal GST-I
    Gene namesi
    Name:Mgst1
    Synonyms:Gst12
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi70927. Mgst1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical part of cell Source: RGD
    2. endoplasmic reticulum Source: HGNC
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB
    5. mitochondrial inner membrane Source: Ensembl
    6. mitochondrial outer membrane Source: UniProtKB-SubCell
    7. mitochondrion Source: RGD
    8. nucleus Source: RGD
    9. peroxisomal membrane Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi76 – 761H → Q: Decreased enzyme activity. 1 Publication
    Mutagenesisi81 – 811E → Q: Loss of enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 155154Microsomal glutathione S-transferase 1PRO_0000217739Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421N6-acetyllysineBy similarity
    Modified residuei55 – 551N6-acetyllysineBy similarity
    Modified residuei60 – 601N6-acetyllysineBy similarity
    Modified residuei93 – 931Nitrated tyrosine1 Publication

    Post-translational modificationi

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.1 Publication

    Keywords - PTMi

    Acetylation, Nitration

    Proteomic databases

    PaxDbiP08011.
    PRIDEiP08011.

    Expressioni

    Tissue specificityi

    Highest in the liver, followed by kidney and testis and much lower in seminal vesicles, spleen, lung and brain.

    Gene expression databases

    ArrayExpressiP08011.
    GenevestigatoriP08011.

    Interactioni

    Subunit structurei

    Homotrimer; The trimer binds only one molecule of glutathione.1 Publication

    Protein-protein interaction databases

    MINTiMINT-4575717.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2H8Aelectron microscopy3.20A2-155[»]
    ProteinModelPortaliP08011.
    SMRiP08011. Positions 10-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08011.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini3 – 97Lumenal
    Topological domaini34 – 6229CytoplasmicAdd
    BLAST
    Topological domaini97 – 993Lumenal
    Topological domaini124 – 1285Cytoplasmic
    Topological domaini149 – 1557Lumenal

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3324HelicalAdd
    BLAST
    Transmembranei63 – 9634HelicalAdd
    BLAST
    Transmembranei100 – 12324HelicalAdd
    BLAST
    Transmembranei129 – 14820HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG71159.
    GeneTreeiENSGT00390000011980.
    HOGENOMiHOG000231759.
    HOVERGENiHBG052470.
    InParanoidiP08011.
    KOiK00799.
    OMAiLIHFRIF.
    OrthoDBiEOG7288T0.
    PhylomeDBiP08011.
    TreeFamiTF105327.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08011-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLKQLMDN EVLMAFTSYA TIILAKMMFL SSATAFQRLT NKVFANPEDC    50
    AGFGKGENAK KFLRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL 100
    STALIHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR 150
    SRLYL 155
    Length:155
    Mass (Da):17,472
    Last modified:January 23, 2007 - v3
    Checksum:i8FB62EEDF5A74489
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271M → V AA sequence (PubMed:3932348)Curated
    Sequence conflicti55 – 551K → G AA sequence (PubMed:3932348)Curated
    Sequence conflicti68 – 681K → T AA sequence (PubMed:3932348)Curated
    Sequence conflicti97 – 971G → A AA sequence (PubMed:3932348)Curated
    Sequence conflicti101 – 1011S → P AA sequence (PubMed:3932348)Curated
    Sequence conflicti134 – 1341F → K AA sequence (PubMed:3932348)Curated
    Sequence conflicti141 – 1411T → D AA sequence (PubMed:3932348)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03752 mRNA. Translation: AAA41281.1.
    BC063150 mRNA. Translation: AAH63150.1.
    PIRiA28083.
    RefSeqiNP_599176.1. NM_134349.3.
    UniGeneiRn.2580.

    Genome annotation databases

    EnsembliENSRNOT00000010579; ENSRNOP00000010579; ENSRNOG00000007743.
    GeneIDi171341.
    KEGGirno:171341.
    UCSCiRGD:70927. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03752 mRNA. Translation: AAA41281.1 .
    BC063150 mRNA. Translation: AAH63150.1 .
    PIRi A28083.
    RefSeqi NP_599176.1. NM_134349.3.
    UniGenei Rn.2580.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2H8A electron microscopy 3.20 A 2-155 [» ]
    ProteinModelPortali P08011.
    SMRi P08011. Positions 10-148.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4575717.

    Proteomic databases

    PaxDbi P08011.
    PRIDEi P08011.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010579 ; ENSRNOP00000010579 ; ENSRNOG00000007743 .
    GeneIDi 171341.
    KEGGi rno:171341.
    UCSCi RGD:70927. rat.

    Organism-specific databases

    CTDi 4257.
    RGDi 70927. Mgst1.

    Phylogenomic databases

    eggNOGi NOG71159.
    GeneTreei ENSGT00390000011980.
    HOGENOMi HOG000231759.
    HOVERGENi HBG052470.
    InParanoidi P08011.
    KOi K00799.
    OMAi LIHFRIF.
    OrthoDBi EOG7288T0.
    PhylomeDBi P08011.
    TreeFami TF105327.

    Enzyme and pathway databases

    SABIO-RK P08011.

    Miscellaneous databases

    EvolutionaryTracei P08011.
    NextBioi 622110.
    PROi P08011.

    Gene expression databases

    ArrayExpressi P08011.
    Genevestigatori P08011.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene expression of rat and human microsomal glutathione S-transferases."
      Dejong J.L., Morgenstern R., Joernvall H., Depierre J.W., Tu C.-P.D.
      J. Biol. Chem. 263:8430-8436(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    3. "Microsomal glutathione transferase. Primary structure."
      Morgenstern R., Depierre J.W., Joernvall H.
      J. Biol. Chem. 260:13976-13983(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-155.
    4. "The distribution of microsomal glutathione transferase among different organelles, different organs, and different organisms."
      Morgenstern R., Lundqvist G., Andersson G., Balk L., Depierre J.W.
      Biochem. Pharmacol. 33:3609-3614(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    5. "Reactivity of cysteine-49 and its influence on the activation of microsomal glutathione transferase 1: evidence for subunit interaction."
      Svensson R., Rinaldi R., Swedmark S., Morgenstern R.
      Biochemistry 39:15144-15149(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE OF CYS-50 IN ENZYME REGULATION.
    6. "Nitration of tyrosine 92 mediates the activation of rat microsomal glutathione s-transferase by peroxynitrite."
      Ji Y., Neverova I., Van Eyk J.E., Bennett B.M.
      J. Biol. Chem. 281:1986-1991(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NITRATION AT TYR-93, ENZYME REGULATION.
    7. "Structural basis for detoxification and oxidative stress protection in membranes."
      Holm P.J., Bhakat P., Jegerschold C., Gyobu N., Mitsuoka K., Fujiyoshi Y., Morgenstern R., Hebert H.
      J. Mol. Biol. 360:934-945(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 2-154 IN COMPLEX WITH GLUTATHIONE, SUBUNIT, SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY, MUTAGENESIS OF HIS-76 AND GLU-81, GLUTATHIONE BINDING SITES.

    Entry informationi

    Entry nameiMGST1_RAT
    AccessioniPrimary (citable) accession number: P08011
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3