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Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide, except in the testis. Activation also occurs via nitration of Tyr-93 by peroxynitrite.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Glutathione1 Publication1
Sitei50Activates the enzyme when modified1
Binding sitei73Glutathione1 Publication1
Binding sitei74Glutathione1 Publication1
Binding sitei76Glutathione1 Publication1
Binding sitei81Glutathione1 Publication1
Binding sitei121Glutathione1 Publication1

GO - Molecular functioni

  • glutathione binding Source: UniProtKB
  • glutathione peroxidase activity Source: Ensembl
  • glutathione transferase activity Source: UniProtKB
  • identical protein binding Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

  • cellular response to lipid hydroperoxide Source: Ensembl
  • glutathione metabolic process Source: Ensembl
  • Leydig cell differentiation Source: RGD
  • protein homotrimerization Source: UniProtKB
  • response to drug Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organonitrogen compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 5301.
ReactomeiR-RNO-156590. Glutathione conjugation.
R-RNO-5423646. Aflatoxin activation and detoxification.
R-RNO-6798695. Neutrophil degranulation.
SABIO-RKP08011.

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:Mgst1
Synonyms:Gst12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi70927. Mgst1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 9Lumenal7
Transmembranei10 – 33HelicalAdd BLAST24
Topological domaini34 – 62CytoplasmicAdd BLAST29
Transmembranei63 – 96HelicalAdd BLAST34
Topological domaini97 – 99Lumenal3
Transmembranei100 – 123HelicalAdd BLAST24
Topological domaini124 – 128Cytoplasmic5
Transmembranei129 – 148HelicalAdd BLAST20
Topological domaini149 – 155Lumenal7

GO - Cellular componenti

  • apical part of cell Source: RGD
  • endoplasmic reticulum Source: HGNC
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
  • nucleus Source: RGD
  • peroxisomal membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76H → Q: Decreased enzyme activity. 1 Publication1
Mutagenesisi81E → Q: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002177392 – 155Microsomal glutathione S-transferase 1Add BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei55N6-acetyllysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei93Nitrated tyrosine1 Publication1

Post-translational modificationi

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.1 Publication

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

PaxDbiP08011.
PRIDEiP08011.

PTM databases

iPTMnetiP08011.
PhosphoSitePlusiP08011.

Expressioni

Tissue specificityi

Highest in the liver, followed by kidney and testis and much lower in seminal vesicles, spleen, lung and brain.

Gene expression databases

BgeeiENSRNOG00000007743.
ExpressionAtlasiP08011. baseline and differential.
GenevisibleiP08011. RN.

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.1 Publication

GO - Molecular functioni

  • identical protein binding Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

MINTiMINT-4575717.
STRINGi10116.ENSRNOP00000010579.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H8Aelectron microscopy3.20A2-155[»]
ProteinModelPortaliP08011.
SMRiP08011.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08011.

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXE1. Eukaryota.
ENOG4111VJG. LUCA.
GeneTreeiENSGT00390000011980.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiP08011.
KOiK00799.
OMAiIKMLAMS.
OrthoDBiEOG091G14I4.
PhylomeDBiP08011.
TreeFamiTF105327.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLKQLMDN EVLMAFTSYA TIILAKMMFL SSATAFQRLT NKVFANPEDC
60 70 80 90 100
AGFGKGENAK KFLRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL
110 120 130 140 150
STALIHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR

SRLYL
Length:155
Mass (Da):17,472
Last modified:January 23, 2007 - v3
Checksum:i8FB62EEDF5A74489
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27M → V AA sequence (PubMed:3932348).Curated1
Sequence conflicti55K → G AA sequence (PubMed:3932348).Curated1
Sequence conflicti68K → T AA sequence (PubMed:3932348).Curated1
Sequence conflicti97G → A AA sequence (PubMed:3932348).Curated1
Sequence conflicti101S → P AA sequence (PubMed:3932348).Curated1
Sequence conflicti134F → K AA sequence (PubMed:3932348).Curated1
Sequence conflicti141T → D AA sequence (PubMed:3932348).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03752 mRNA. Translation: AAA41281.1.
BC063150 mRNA. Translation: AAH63150.1.
PIRiA28083.
RefSeqiNP_599176.1. NM_134349.3.
UniGeneiRn.2580.

Genome annotation databases

EnsembliENSRNOT00000010579; ENSRNOP00000010579; ENSRNOG00000007743.
GeneIDi171341.
KEGGirno:171341.
UCSCiRGD:70927. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03752 mRNA. Translation: AAA41281.1.
BC063150 mRNA. Translation: AAH63150.1.
PIRiA28083.
RefSeqiNP_599176.1. NM_134349.3.
UniGeneiRn.2580.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H8Aelectron microscopy3.20A2-155[»]
ProteinModelPortaliP08011.
SMRiP08011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4575717.
STRINGi10116.ENSRNOP00000010579.

PTM databases

iPTMnetiP08011.
PhosphoSitePlusiP08011.

Proteomic databases

PaxDbiP08011.
PRIDEiP08011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010579; ENSRNOP00000010579; ENSRNOG00000007743.
GeneIDi171341.
KEGGirno:171341.
UCSCiRGD:70927. rat.

Organism-specific databases

CTDi4257.
RGDi70927. Mgst1.

Phylogenomic databases

eggNOGiENOG410IXE1. Eukaryota.
ENOG4111VJG. LUCA.
GeneTreeiENSGT00390000011980.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiP08011.
KOiK00799.
OMAiIKMLAMS.
OrthoDBiEOG091G14I4.
PhylomeDBiP08011.
TreeFamiTF105327.

Enzyme and pathway databases

BRENDAi2.5.1.18. 5301.
ReactomeiR-RNO-156590. Glutathione conjugation.
R-RNO-5423646. Aflatoxin activation and detoxification.
R-RNO-6798695. Neutrophil degranulation.
SABIO-RKP08011.

Miscellaneous databases

EvolutionaryTraceiP08011.
PROiP08011.

Gene expression databases

BgeeiENSRNOG00000007743.
ExpressionAtlasiP08011. baseline and differential.
GenevisibleiP08011. RN.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGST1_RAT
AccessioniPrimary (citable) accession number: P08011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.