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Protein

Glutathione S-transferase Yb-3

Gene

Gstm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161SubstrateBy similarity

GO - Molecular functioni

  1. glutathione binding Source: Ensembl
  2. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular detoxification of nitrogen compound Source: Ensembl
  2. cellular response to caffeine Source: Ensembl
  3. glutathione metabolic process Source: Ensembl
  4. negative regulation of ryanodine-sensitive calcium-release channel activity Source: Ensembl
  5. nitrobenzene metabolic process Source: Ensembl
  6. positive regulation of ryanodine-sensitive calcium-release channel activity Source: Ensembl
  7. regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: Ensembl
  8. xenobiotic catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_251519. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Yb-3 (EC:2.5.1.18)
Alternative name(s):
Chain 4
GST Yb3
GST class-mu 3
Gene namesi
Name:Gstm3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi621287. Gstm3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. sarcoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 218217Glutathione S-transferase Yb-3PRO_0000185833Add
BLAST

Proteomic databases

PaxDbiP08009.
PRIDEiP08009.

2D gel databases

World-2DPAGE0004:P08009.

PTM databases

PhosphoSiteiP08009.

Expressioni

Gene expression databases

GenevestigatoriP08009.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

MINTiMINT-4565175.
STRINGi10116.ENSRNOP00000025689.

Structurei

3D structure databases

ProteinModelPortaliP08009.
SMRiP08009. Positions 1-217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8887GST N-terminalAdd
BLAST
Domaini90 – 208119GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 82Glutathione bindingBy similarity
Regioni46 – 505Glutathione bindingBy similarity
Regioni59 – 602Glutathione bindingBy similarity
Regioni72 – 732Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG300089.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP08009.
KOiK00799.
OMAiMAIWGSK.
OrthoDBiEOG7KH9M3.
PhylomeDBiP08009.
TreeFamiTF353040.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08009-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPMTLGYWDI RGLAHAIRLL LEYTDSSYEE KRYTMGDAPD FDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGSHKI TQSNAILRYL GRKHNLCGET EEERIRVDIL
110 120 130 140 150
ENQLMDNRMV LARLCYNPDF EKLKPGYLEQ LPGMMRLYSE FLGKRPWFAG
160 170 180 190 200
DKITFVDFIA YDVLERNQVF EATCLDAFPN LKDFIARFEG LKKISDYMKS
210
SRFLPRPLFT KMAIWGSK
Length:218
Mass (Da):25,681
Last modified:January 23, 2007 - v2
Checksum:iBBC0B61288AB069A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02744 mRNA. Translation: AAA41292.1.
L35330 Genomic DNA. Translation: AAB00123.1.
BC059130 mRNA. Translation: AAH59130.1.
PIRiA29036.
RefSeqiNP_112416.1. NM_031154.1.
UniGeneiRn.6036.

Genome annotation databases

EnsembliENSRNOT00000025689; ENSRNOP00000025689; ENSRNOG00000018937.
GeneIDi81869.
KEGGirno:81869.
UCSCiRGD:621287. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02744 mRNA. Translation: AAA41292.1.
L35330 Genomic DNA. Translation: AAB00123.1.
BC059130 mRNA. Translation: AAH59130.1.
PIRiA29036.
RefSeqiNP_112416.1. NM_031154.1.
UniGeneiRn.6036.

3D structure databases

ProteinModelPortaliP08009.
SMRiP08009. Positions 1-217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4565175.
STRINGi10116.ENSRNOP00000025689.

PTM databases

PhosphoSiteiP08009.

2D gel databases

World-2DPAGE0004:P08009.

Proteomic databases

PaxDbiP08009.
PRIDEiP08009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025689; ENSRNOP00000025689; ENSRNOG00000018937.
GeneIDi81869.
KEGGirno:81869.
UCSCiRGD:621287. rat.

Organism-specific databases

CTDi68312.
RGDi621287. Gstm3.

Phylogenomic databases

eggNOGiNOG300089.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP08009.
KOiK00799.
OMAiMAIWGSK.
OrthoDBiEOG7KH9M3.
PhylomeDBiP08009.
TreeFamiTF353040.

Enzyme and pathway databases

ReactomeiREACT_251519. Glutathione conjugation.

Miscellaneous databases

NextBioi615787.
PROiP08009.

Gene expression databases

GenevestigatoriP08009.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Selective expression of a unique glutathione S-transferase Yb3 gene in rat brain."
    Abramovitz M., Listowsky I.
    J. Biol. Chem. 262:7770-7773(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Brain and testis selective expression of the glutathione S-transferase Yb3 subunit is governed by tandem direct repeat octamer motifs in the 5'-flanking region of its gene."
    Abramovitz M., Testori A., Angelov I.V., Darmon A., Listowsky I.
    Brain Res. Mol. Brain Res. 28:37-46(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Tail.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  4. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 19-50; 53-69; 137-144 AND 153-182, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.

Entry informationi

Entry nameiGSTM4_RAT
AccessioniPrimary (citable) accession number: P08009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Yb subclass selectively binds steroid hormones.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.