P08009 (GSTM4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase Yb-3
EC=2.5.1.18

Alternative name(s):
Chain 4
GST Yb3
GST class-mu 3

Gene names

Name:

Gstm3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	Yb subclass selectively binds steroid hormones.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from electronic annotation. Source: Compara xenobiotic catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 218	217	Glutathione S-transferase Yb-3		PRO_0000185833
Regions
	Domain	2 – 88	87	GST N-terminal
	Domain	90 – 208	119	GST C-terminal
	Region	7 – 8	2	Glutathione binding By similarity
	Region	46 – 50	5	Glutathione binding By similarity
	Region	59 – 60	2	Glutathione binding By similarity
	Region	72 – 73	2	Glutathione binding By similarity
Sites
	Binding site	116	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08009 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: BBC0B61288AB069A	FASTA	218	25,681
	10 20 30 40 50 60 MPMTLGYWDI RGLAHAIRLL LEYTDSSYEE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL 70 80 90 100 110 120 PYLIDGSHKI TQSNAILRYL GRKHNLCGET EEERIRVDIL ENQLMDNRMV LARLCYNPDF 130 140 150 160 170 180 EKLKPGYLEQ LPGMMRLYSE FLGKRPWFAG DKITFVDFIA YDVLERNQVF EATCLDAFPN 190 200 210 LKDFIARFEG LKKISDYMKS SRFLPRPLFT KMAIWGSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selective expression of a unique glutathione S-transferase Yb3 gene in rat brain." Abramovitz M., Listowsky I. J. Biol. Chem. 262:7770-7773(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Brain and testis selective expression of the glutathione S-transferase Yb3 subunit is governed by tandem direct repeat octamer motifs in the 5'-flanking region of its gene." Abramovitz M., Testori A., Angelov I.V., Darmon A., Listowsky I. Brain Res. Mol. Brain Res. 28:37-46(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Tail.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[4]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 19-50; 53-69; 137-144 AND 153-182, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02744 mRNA. Translation: AAA41292.1. L35330 Genomic DNA. Translation: AAB00123.1. BC059130 mRNA. Translation: AAH59130.1.
IPI	IPI00230942.
PIR	A29036.
RefSeq	NP_112416.1. NM_031154.1.
UniGene	Rn.6036.
3D structure databases
ProteinModelPortal	P08009.
SMR	P08009. Positions 1-217.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000025689.
PTM databases
PhosphoSite	P08009.
2D gel databases
World-2DPAGE	0004:P08009.
Proteomic databases
PaxDb	P08009.
PRIDE	P08009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025689; ENSRNOP00000025689; ENSRNOG00000018937.
GeneID	81869.
KEGG	rno:81869.
UCSC	RGD:621287. rat.
Organism-specific databases
CTD	68312.
RGD	621287. Gstm3.
Phylogenomic databases
eggNOG	NOG300089.
GeneTree	ENSGT00550000074559.
HOGENOM	HOG000115735.
HOVERGEN	HBG106842.
InParanoid	P08009.
KO	K00799.
OMA	RVNILEN.
OrthoDB	EOG47D9H2.
Gene expression databases
Genevestigator	P08009.
GermOnline	ENSRNOG00000018937. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR003081. GST_mu. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01267. GSTRNSFRASEM.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615787.

Entry information

Entry name

GSTM4_RAT

Accession

Primary (citable) accession number: P08009

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program