P08004 (CHS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1

Gene names

Name:	CHS1
Ordered Locus Names:	YNL192W
ORF Names:	N1404

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Septum formation and repair, especially under certain adverse conditions.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Enzyme regulation	Requires proteolytic activation.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell budding Traceable author statement. Source: SGD cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro cytokinesis, completion of separation Inferred from mutant phenotype. Source: SGD
Cellular component	chitosome Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD
Molecular function	chitin synthase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAN1	P04817	1	EBI-4618,EBI-3993
COX20	Q04935	1	EBI-4618,EBI-36910
GAP1	P19145	1	EBI-4618,EBI-7314
PMP2	P40975	1	EBI-4618,EBI-2043041
PMP3	P87284	1	EBI-4618,EBI-13555

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1131	1131	Chitin synthase 1		PRO_0000193727
Regions
	Transmembrane	795 – 815	21	Helical; Potential
	Transmembrane	833 – 853	21	Helical; Potential
	Transmembrane	866 – 886	21	Helical; Potential
	Transmembrane	914 – 934	21	Helical; Potential
	Transmembrane	942 – 962	21	Helical; Potential
	Transmembrane	1042 – 1062	21	Helical; Potential
	Transmembrane	1101 – 1121	21	Helical; Potential
Amino acid modifications
	Modified residue	34	1	Phosphoserine Ref.5
	Modified residue	270	1	Phosphoserine Ref.5 Ref.7
	Modified residue	299	1	Phosphoserine Ref.5
	Modified residue	305	1	Phosphoserine Ref.6
	Modified residue	318	1	Phosphoserine Ref.5
	Modified residue	328	1	Phosphothreonine Ref.4 Ref.5
	Modified residue	358	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	815	1	V → F in AAA34491. Ref.1
	Sequence conflict	815	1	V → F in CAA96086. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08004 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: C1856F3C0C857E07	FASTA	1,131	129,871
	10 20 30 40 50 60 MSDQNNRSRN EYHSNRKNEP SYELQNAHSG LFHSSNEELT NRNQRYTNQN ASMGSFTPVQ 70 80 90 100 110 120 SLQFPEQSQQ TNMLYNGDDG NNNTINDNER DIYGGFVNHH RQRPPPATAE YNDVFNTNSQ 130 140 150 160 170 180 QLPSEHQYNN VPSYPLPSIN VIQTTPELIH NGSQTMATPI ERPFFNENDY YYNNRNSRTS 190 200 210 220 230 240 PSIASSSDGY ADQEARPILE QPNNNMNSGN IPQYHDQPFG YNNGYHGLQA KDYYDDPEGG 250 260 270 280 290 300 YIDQRGDDYQ INSYLGRNGE MVDPYDYENS LRHMTPMERR EYLHDDSRPV NDGKEELDSV 310 320 330 340 350 360 KSGYSHRDLG EYDKDDFSRD DEYDDLNTID KLQFQANGVP ASSSVSSIGS KESDIIVSND 370 380 390 400 410 420 NLTANRALKR SGTEIRKFKL WNGNFVFDSP ISKTLLDQYA TTTENANTLP NEFKFMRYQA 430 440 450 460 470 480 VTCEPNQLAE KNFTVRQLKY LTPRETELML VVTMYNEDHI LLGRTLKGIM DNVKYMVKKK 490 500 510 520 530 540 NSSTWGPDAW KKIVVCIISD GRSKINERSL ALLSSLGCYQ DGFAKDEINE KKVAMHVYEH 550 560 570 580 590 600 TTMINITNIS ESEVSLECNQ GTVPIQLLFC LKEQNQKKIN SHRWAFEGFA ELLRPNIVTL 610 620 630 640 650 660 LDAGTMPGKD SIYQLWREFR NPNVGGACGE IRTDLGKRFV KLLNPLVASQ NFEYKMSNIL 670 680 690 700 710 720 DKTTESNFGF ITVLPGAFSA YRFEAVRGQP LQKYFYGEIM ENEGFHFFSS NMYLAEDRIL 730 740 750 760 770 780 CFEVVTKKNC NWILKYCRSS YASTDVPERV PEFILQRRRW LNGSFFASVY SFCHFYRVWS 790 800 810 820 830 840 SGHNIGRKLL LTVEFFYLFF NTLISWFSLS SFFLVFRILT VSIALAYHSA FNVLSVIFLW 850 860 870 880 890 900 LYGICTLSTF ILSLGNKPKS TEKFYVLTCV IFAVMMIYMI FCSIFMSVKS FQNILKNDTI 910 920 930 940 950 960 SFEGLITTEA FRDIVISLGS TYCLYLISSI IYLQPWHMLT SFIQYILLSP SYINVLNIYA 970 980 990 1000 1010 1020 FCNVHDLSWG TKGAMANPLG KINTTEDGTF KMEVLVSSSE IQANYDKYLK VLNDFDPKSE 1030 1040 1050 1060 1070 1080 SRPTEPSYDE KKTGYYANVR SLVIIFWVIT NFIIVAVVLE TGGIADYIAM KSISTDDTLE 1090 1100 1110 1120 1130 TAKKAEIPLM TSKASIYFNV ILWLVALSAL IRFIGCSIYM IVRFFKKVTF R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo." Bulawa C.E., Slater M., Cabib E., Au-Young J., Sburlati A., Adair W.L. Jr., Robbins P.W. Cell 46:213-225(1986) [PubMed: 2941152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 815. Strain: ATCC 204508 / S288c.
[4]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-328, MASS SPECTROMETRY. Strain: SUB592.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-270; SER-299; SER-318 AND THR-328, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270 AND SER-358, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14045 Genomic DNA. Translation: AAA34491.1. Z71468 Genomic DNA. Translation: CAA96086.1. BK006947 Genomic DNA. Translation: DAA10361.2.
PIR	A23944.
RefSeq	NP_014207.2. NM_001183030.2.
3D structure databases
ProteinModelPortal	P08004.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4680N.
IntAct	P08004. 11 interactions.
MINT	MINT-557753.
STRING	P08004.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Proteomic databases
PeptideAtlas	P08004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	855529.
KEGG	sce:YNL192W.
NMPDR	fig|4932.3.peg.5272.
Organism-specific databases
CYGD	YNL192w.
SGD	S000005136. CHS1.
Phylogenomic databases
eggNOG	fuNOG04531.
GeneTree	EFGT00050000001909.
HOGENOM	HBG327157.
OMA	HILLGRT.
OrthoDB	EOG4GMZ52.
Gene expression databases
ArrayExpress	P08004.
Genevestigator	P08004.
GermOnline	YNL192W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
KO	K00698.
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	979574.

Entry information

Entry name

CHS1_YEAST

Accession

Primary (citable) accession number: P08004
Secondary accession number(s): D6W0Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	September 21, 2011
Last modified:	December 14, 2011
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program