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Protein

Protein disulfide-isomerase A4

Gene

Pdia4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A4 (EC:5.3.4.1)
Alternative name(s):
Endoplasmic reticulum resident protein 72
Short name:
ER protein 72
Short name:
ERp-72
Short name:
ERp72
Gene namesi
Name:Pdia4
Synonyms:Cai, Erp72
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104864. Pdia4.

Subcellular locationi

  • Endoplasmic reticulum lumen By similarity
  • Melanosome By similarity

GO - Cellular componenti

  • cell surface Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum chaperone complex Source: ParkinsonsUK-UCL
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003423021 – 638Protein disulfide-isomerase A4Add BLAST618

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi84 ↔ 87Redox-activePROSITE-ProRule annotation
Disulfide bondi199 ↔ 202Redox-activePROSITE-ProRule annotation
Modified residuei359N6-acetyllysineBy similarity1
Disulfide bondi548 ↔ 551Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

EPDiP08003.
MaxQBiP08003.
PaxDbiP08003.
PeptideAtlasiP08003.
PRIDEiP08003.

PTM databases

iPTMnetiP08003.
PhosphoSitePlusiP08003.
SwissPalmiP08003.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025823.
CleanExiMM_PDIA4.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Protein-protein interaction databases

BioGridi198446. 1 interactor.
IntActiP08003. 5 interactors.
MINTiMINT-4106888.
STRINGi10090.ENSMUSP00000076521.

Structurei

Secondary structure

1638
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi52 – 54Combined sources3
Beta strandi57 – 59Combined sources3
Turni62 – 64Combined sources3
Helixi65 – 69Combined sources5
Beta strandi73 – 79Combined sources7
Helixi85 – 88Combined sources4
Helixi91 – 102Combined sources12
Beta strandi103 – 106Combined sources4
Beta strandi109 – 113Combined sources5
Turni115 – 117Combined sources3
Helixi119 – 124Combined sources6
Beta strandi129 – 137Combined sources9
Beta strandi140 – 143Combined sources4
Helixi150 – 161Combined sources12
Beta strandi162 – 164Combined sources3
Beta strandi171 – 173Combined sources3
Turni177 – 179Combined sources3
Turni181 – 183Combined sources3
Helixi184 – 186Combined sources3
Beta strandi188 – 195Combined sources8
Helixi200 – 218Combined sources19
Beta strandi219 – 221Combined sources3
Beta strandi225 – 229Combined sources5
Turni230 – 232Combined sources3
Helixi234 – 239Combined sources6
Beta strandi244 – 254Combined sources11
Beta strandi256 – 258Combined sources3
Helixi265 – 277Combined sources13
Beta strandi519 – 522Combined sources4
Turni525 – 527Combined sources3
Helixi528 – 532Combined sources5
Beta strandi537 – 543Combined sources7
Helixi550 – 565Combined sources16
Beta strandi568 – 576Combined sources9
Turni578 – 580Combined sources3
Beta strandi591 – 599Combined sources9
Helixi619 – 627Combined sources9
Beta strandi628 – 630Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DJ1NMR-A46-172[»]
2DJ2NMR-A171-277[»]
2DJ3NMR-A519-638[»]
ProteinModelPortaliP08003.
SMRiP08003.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08003.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 162Thioredoxin 1PROSITE-ProRule annotationAdd BLAST142
Domaini162 – 294Thioredoxin 2PROSITE-ProRule annotationAdd BLAST133
Domaini498 – 629Thioredoxin 3PROSITE-ProRule annotationAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi635 – 638Prevents secretion from ER4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 50Asp/Glu-rich (acidic)Add BLAST12

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 3 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiP08003.
KOiK09582.
PhylomeDBiP08003.
TreeFamiTF106382.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR017068. Protein_diS-isomerase_A4.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 3 hits.
[Graphical view]
PIRSFiPIRSF036862. Disulphide_isom_A4. 1 hit.
SUPFAMiSSF52833. SSF52833. 5 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 3 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRKAWLLV LLLALTQLLA AASAGDAHED TSDTENATEE EEEEDDDDLE
60 70 80 90 100
VKEENGVWVL NDGNFDNFVA DKDTVLLEFY APWCGHCKQF APEYEKIAST
110 120 130 140 150
LKDNDPPIAV AKIDATSASM LASKFDVSGY PTIKILKKGQ AVDYDGSRTQ
160 170 180 190 200
EEIVAKVREV SQPDWTPPPE VTLSLTKDNF DDVVNNADII LVEFYAPWCG
210 220 230 240 250
HCKKLAPEYE KAAKELSKRS PPIPLAKVDA TEQTDLAKRF DVSGYPTLKI
260 270 280 290 300
FRKGRPFDYN GPREKYGIVD YMIEQSGPPS KEILTLKQVQ EFLKDGDDVV
310 320 330 340 350
IIGLFQGDGD PAYLQYQDAA NNLREDYKFH HTFSPEIAKF LKVSLGKLVL
360 370 380 390 400
THPEKFQSKY EPRFHVMDVQ GSTEASAIKD YVVKHALPLV GHRKTSNDAK
410 420 430 440 450
RYSKRPLVVV YYSVDFSFDY RAATQFWRNK VLEVAKDFPE YTFAIADEED
460 470 480 490 500
YATEVKDLGL SESGEDVNAA ILDESGKKFA MEPEEFDSDT LREFVTAFKK
510 520 530 540 550
GKLKPVIKSQ PVPKNNKGPV KVVVGKTFDA IVMDPKKDVL IEFYAPWCGH
560 570 580 590 600
CKQLEPIYTS LGKKYKGQKD LVIAKMDATA NDITNDQYKV EGFPTIYFAP
610 620 630
SGDKKNPIKF EGGNRDLEHL SKFIDEHATK RSRTKEEL
Length:638
Mass (Da):71,982
Last modified:November 30, 2010 - v3
Checksum:i2DDE2CE80BD77F6E
GO

Sequence cautioni

The sequence CAA68777 differs from that shown. Reason: Frameshift at position 544.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28H → Q in AAA39907 (PubMed:2295602).Curated1
Sequence conflicti28H → Q in BAE27045 (PubMed:16141072).Curated1
Sequence conflicti28H → Q in AAI41079 (PubMed:15489334).Curated1
Sequence conflicti39E → EEEE in BAC25863 (PubMed:16141072).Curated1
Sequence conflicti39E → EEEE in BAE41134 (PubMed:16141072).Curated1
Sequence conflicti39E → EEEE in BAE36446 (PubMed:16141072).Curated1
Sequence conflicti39E → EEEE in BAE29664 (PubMed:16141072).Curated1
Sequence conflicti468N → S in BAC25863 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05186 mRNA. Translation: AAA39907.1.
AK028292 mRNA. Translation: BAC25863.1.
AK146288 mRNA. Translation: BAE27045.1.
AK150566 mRNA. Translation: BAE29664.1.
AK161534 mRNA. Translation: BAE36446.1.
AK169387 mRNA. Translation: BAE41134.1.
BC066857 mRNA. Translation: AAH66857.1.
BC141078 mRNA. Translation: AAI41079.1.
Y00884 mRNA. Translation: CAA68777.1. Frameshift.
PIRiB34930. ISMSER.
S06318.
RefSeqiNP_033917.2. NM_009787.2.
UniGeneiMm.2442.

Genome annotation databases

GeneIDi12304.
KEGGimmu:12304.
UCSCiuc009btf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05186 mRNA. Translation: AAA39907.1.
AK028292 mRNA. Translation: BAC25863.1.
AK146288 mRNA. Translation: BAE27045.1.
AK150566 mRNA. Translation: BAE29664.1.
AK161534 mRNA. Translation: BAE36446.1.
AK169387 mRNA. Translation: BAE41134.1.
BC066857 mRNA. Translation: AAH66857.1.
BC141078 mRNA. Translation: AAI41079.1.
Y00884 mRNA. Translation: CAA68777.1. Frameshift.
PIRiB34930. ISMSER.
S06318.
RefSeqiNP_033917.2. NM_009787.2.
UniGeneiMm.2442.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DJ1NMR-A46-172[»]
2DJ2NMR-A171-277[»]
2DJ3NMR-A519-638[»]
ProteinModelPortaliP08003.
SMRiP08003.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198446. 1 interactor.
IntActiP08003. 5 interactors.
MINTiMINT-4106888.
STRINGi10090.ENSMUSP00000076521.

PTM databases

iPTMnetiP08003.
PhosphoSitePlusiP08003.
SwissPalmiP08003.

Proteomic databases

EPDiP08003.
MaxQBiP08003.
PaxDbiP08003.
PeptideAtlasiP08003.
PRIDEiP08003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12304.
KEGGimmu:12304.
UCSCiuc009btf.1. mouse.

Organism-specific databases

CTDi9601.
MGIiMGI:104864. Pdia4.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiP08003.
KOiK09582.
PhylomeDBiP08003.
TreeFamiTF106382.

Miscellaneous databases

ChiTaRSiPdia4. mouse.
EvolutionaryTraceiP08003.
PROiP08003.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025823.
CleanExiMM_PDIA4.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR017068. Protein_diS-isomerase_A4.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 3 hits.
[Graphical view]
PIRSFiPIRSF036862. Disulphide_isom_A4. 1 hit.
SUPFAMiSSF52833. SSF52833. 5 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 3 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA4_MOUSE
AccessioniPrimary (citable) accession number: P08003
Secondary accession number(s): P15841
, Q3TT79, Q3UJW0, Q6NXW4, Q8BMT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 30, 2010
Last modified: November 2, 2016
This is version 166 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.