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Protein

Acrosin

Gene

ACR

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Charge relay systemBy similarity1
Active sitei140Charge relay systemBy similarity1
Active sitei238Charge relay systemBy similarity1

GO - Molecular functioni

  • amidase activity Source: UniProtKB
  • drug binding Source: UniProtKB
  • fucose binding Source: UniProtKB
  • mannose binding Source: UniProtKB
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.10 6170

Protein family/group databases

MEROPSiS01.223

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Alternative name(s):
53 kDa fucose-binding protein
Cleaved into the following 2 chains:
Gene namesi
Name:ACR
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243910

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 163 PublicationsAdd BLAST16
ChainiPRO_000002752617 – 415AcrosinAdd BLAST399
ChainiPRO_000002752717 – 39Acrosin light chainAdd BLAST23
ChainiPRO_000002752840 – 338Acrosin heavy chainAdd BLAST299
PropeptideiPRO_0000027529339 – 415Pro-richAdd BLAST77

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi19N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi22 ↔ 152Interchain (between light and heavy chains)PROSITE-ProRule annotation1 Publication
Disulfide bondi26 ↔ 160Interchain (between light and heavy chains)PROSITE-ProRule annotation1 Publication
Disulfide bondi71 ↔ 87PROSITE-ProRule annotation1 Publication
Disulfide bondi175 ↔ 244PROSITE-ProRule annotation1 Publication
Disulfide bondi207 ↔ 223PROSITE-ProRule annotation1 Publication
Glycosylationi208N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi234 ↔ 264PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP08001
PeptideAtlasiP08001
PRIDEiP08001

PTM databases

iPTMnetiP08001

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000021991

Chemistry databases

BindingDBiP08001

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 27Combined sources4
Beta strandi54 – 61Combined sources8
Turni62 – 65Combined sources4
Beta strandi66 – 77Combined sources12
Beta strandi80 – 83Combined sources4
Helixi86 – 88Combined sources3
Beta strandi96 – 102Combined sources7
Beta strandi108 – 110Combined sources3
Beta strandi119 – 128Combined sources10
Turni134 – 137Combined sources4
Beta strandi142 – 148Combined sources7
Beta strandi174 – 181Combined sources8
Beta strandi183 – 186Combined sources4
Beta strandi195 – 202Combined sources8
Helixi204 – 208Combined sources5
Turni210 – 215Combined sources6
Beta strandi221 – 225Combined sources5
Beta strandi241 – 245Combined sources5
Beta strandi253 – 260Combined sources8
Beta strandi262 – 266Combined sources5
Beta strandi271 – 276Combined sources6
Helixi277 – 279Combined sources3
Helixi280 – 287Combined sources8
Helixi289 – 293Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIZX-ray2.90A40-302[»]
L17-39[»]
ProteinModelPortaliP08001
SMRiP08001
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08001

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 288Peptidase S1PROSITE-ProRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOVERGENiHBG013304
InParanoidiP08001
KOiK01317

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR012267 Pept_S1A_acrosin
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24252 PTHR24252, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001141 Acrosin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPTAVLLVL AVSVAARDNA TCDGPCGLRF RQKLESGMRV VGGMSAEPGA
60 70 80 90 100
WPWMVSLQIF MYHNNRRYHT CGGILLNSHW VLTAAHCFKN KKKVTDWRLI
110 120 130 140 150
FGANEVVWGS NKPVKPPLQE RFVEEIIIHE KYVSGLEIND IALIKITPPV
160 170 180 190 200
PCGPFIGPGC LPQFKAGPPR APQTCWVTGW GYLKEKGPRT SPTLQEARVA
210 220 230 240 250
LIDLELCNST RWYNGRIRST NVCAGYPRGK IDTCQGDSGG PLMCRDRAEN
260 270 280 290 300
TFVVVGITSW GVGCARAKRP GVYTSTWPYL NWIASKIGSN ALQMVQLGTP
310 320 330 340 350
PRPSTPAPPV RPPSVQTPVR PPWYFQRPPG PSQQPGSRPR PPAPPPAPPP
360 370 380 390 400
PPPPPPPPPP PPPPPPQQVS AKPPQALSFA KRLQQLIEAL KGTAFSSGRS
410
YYETETTDLQ ELPAS
Length:415
Mass (Da):45,362
Last modified:May 16, 2006 - v5
Checksum:iBAC41900AD43E768
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Missing in CAA32948 (PubMed:2502391).Curated1
Sequence conflicti211R → Q in CAA32948 (PubMed:2502391).Curated1
Sequence conflicti217 – 218IR → VT in CAA32948 (PubMed:2502391).Curated2
Sequence conflicti347A → P in AAA31131 (PubMed:2745422).Curated1
Sequence conflicti389Missing in CAA32948 (PubMed:2502391).Curated1
Sequence conflicti399 – 402RSYY → KELL (PubMed:2502391).Curated4
Sequence conflicti403 – 415Missing (PubMed:2502391).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04950 mRNA Translation: AAA31131.1
X14844 mRNA Translation: CAA32948.1
X58549 Genomic DNA Translation: CAA41440.1
PIRiA34170
RefSeqiNP_999198.1, NM_214033.1
UniGeneiSsc.128

Genome annotation databases

GeneIDi397098
KEGGissc:397098

Similar proteinsi

Entry informationi

Entry nameiACRO_PIG
AccessioniPrimary (citable) accession number: P08001
Secondary accession number(s): P08000, Q29015
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 16, 2006
Last modified: April 25, 2018
This is version 126 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health