Reviewed, UniProtKB/Swiss-Prot P07999 (DHGB_BACME)

Names and origin

Protein names

Recommended name:
    Glucose 1-dehydrogenase B
    EC=1.1.1.47

Gene names

Name:

gdhB

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.
Subunit structure	Homotetramer.
Developmental stage	Expressed during sporulation.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Sporulation
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose 1-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Glucose 1-dehydrogenase B		PRO_0000054614
Regions
	Nucleotide binding	11 – 35	25	NADP By similarity
Sites
	Active site	160	1	Proton acceptor By similarity
	Binding site	146	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07999-1 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: C9281328D634E789	FASTA	262	28,347
	10 20 30 40 50 60 MYKDLEGKVV VITGSSTGLG KSMAIRFATE KAKVVVNYRS KEDEANSVLE EEIKKVGGEA 70 80 90 100 110 120 IAVKGDVTVE SDVINLVQSA IKEFGKLDVM INNAGMENPV SSHEMSLSDW NKVIDTNLTG 130 140 150 160 170 180 AFLGSREAIK YFVENDIKGT VINMSSVHEW KIPWPLFVHY AASKGGMKLM TETLALEYAP 190 200 210 220 230 240 KGIRVNNIGP GAINTPINAE KFADPEQRAD VESMIPMGYI GEPEEIAAVA WLASSEASYV 250 260 TGITLFADGG MTQYPSFQAG RG

References

[1]	"Complete amino acid sequence of glucose dehydrogenase from Bacillus megaterium." Jany K.-D., Ulmer W., Froschle M., Pfleiderer G. FEBS Lett. 165:6-10(1984) [PubMed: 6420184] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Identification and isolation of glucose dehydrogenase genes of Bacillus megaterium M1286 and their expression in Escherichia coli." Heilmann H.J., Maegert H.-J., Gassen H.G. Eur. J. Biochem. 174:485-490(1988) [PubMed: 3134196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52, SEQUENCE REVISION TO 207 AND 258. Strain: M1286.
[3]	"Evidence for an essential histidine residue in glucose dehydrogenase from Bacillus megaterium and sequence analysis of the peptides labeled with bromoacetyl pyridine." Ulmer W., Froschle M., Jany K.-D. Eur. J. Biochem. 136:183-194(1983) [PubMed: 6413208] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-204.
[4]	"Tyrosine modification of glucose dehydrogenase from Bacillus megaterium. Effect of tetranitromethane on the enzyme in the tetrameric and monomeric state." Froschle M., Ulmer W., Jany K.-D. Eur. J. Biochem. 142:533-540(1984) [PubMed: 6432532] [Abstract] Cited for: PROTEIN SEQUENCE OF 218-262.

Cross-references

Sequence databases
PIR	A23260. S01227. S02299.
3D structure databases
HSSP	HSSP built from PDB template 1GCO based on UniProtKB P40288.
SMR	P07999. Positions 1-262.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.47. 325.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHGB_BACME

Accession

Primary (citable) accession number: P07999

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)