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Protein

5-aminolevulinate synthase, nonspecific, mitochondrial

Gene

ALAS1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, erythroid-specific, mitochondrial (ALAS2), 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1), 5-aminolevulinate synthase (ALAS1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212SubstrateBy similarity1
Binding sitei329SubstrateBy similarity1
Binding sitei348SubstrateBy similarity1
Binding sitei381Pyridoxal phosphateBy similarity1
Binding sitei409Pyridoxal phosphateBy similarity1
Binding sitei437Pyridoxal phosphateBy similarity1
Active sitei440By similarity1
Binding sitei469Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei470Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei557SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-GGA-421525. Heme synthesis.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, nonspecific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-H
Alternative name(s):
5-aminolevulinic acid synthase 1
Delta-ALA synthase 1
Delta-aminolevulinate synthase 1
Gene namesi
Name:ALAS1
Synonyms:ALASN
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56MitochondrionAdd BLAST56
ChainiPRO_000000123357 – 6355-aminolevulinate synthase, nonspecific, mitochondrialAdd BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei440N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP07997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG005954.
InParanoidiP07997.
KOiK00643.
PhylomeDBiP07997.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVVRRCPF LARVSQAFLQ KAGPSLLFYA QHCPKMMEAA PPAAARGLAT
60 70 80 90 100
SASRGQQVEE TPAAQPEAKK AKEVAQQNTD GSQPPAGHPP AAAVQSSATK
110 120 130 140 150
CPFLAAQMNH KSSNVFCKAS LELQEDVKEM QVDRKGKEFA KIPTNSVVRN
160 170 180 190 200
TEAEGEEQSG LLKKFKDIML KQRPESVSHL LQDNLPKSVS TFQYDQFFEK
210 220 230 240 250
KIDEKKKDHT YRVFKTVNRK AQIFPMADDY SDSLITKKEV SVWCSNDYLG
260 270 280 290 300
MSRHPRVCGA VMDTLKQHGA GAGGTRNISG TSKFHVDLEK ELADLHGKDA
310 320 330 340 350
ALLFSSCFVA NDSTLFTLAK MLPGCEIYSD SGNHASMIQG IRNSRVPKHI
360 370 380 390 400
FRHNDVNHLR ELLKKSDPST PKIVAFETVH SMDGAVCPLE ELCDVAHEHG
410 420 430 440 450
AITFVDEVHA VGLYGARGGG IGDRDGVMHK MDIISGTLGK AFACVGGYIS
460 470 480 490 500
STSALIDTVR SYAAGFIFTT SLPPMLLAGA LESVRTLKSA EGQVLRRQHQ
510 520 530 540 550
RNVKLMRQML MDAGLPVVHC PSHIIPIRVA DAAKNTEICD KLMSQHSIYV
560 570 580 590 600
QAINYPTVPR GEELLRIAPT PHHTPQMMSY FLEKLLATWK DVGLELKPHS
610 620 630
SAECNFCRRP LHFEVMSERE RSYFSGMSKL LSVSA
Length:635
Mass (Da):69,948
Last modified:January 1, 1990 - v2
Checksum:iEEE708B85C1539BA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53S → A in CAA26595 (PubMed:3839458).Curated1
Sequence conflicti53S → A no nucleotide entry (PubMed:2915978).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02827 mRNA. Translation: CAA26595.1.
X03517, X03627 Genomic DNA. Translation: CAA27223.1.
PIRiA23538. SYCHAL.
RefSeqiNP_001018012.1. NM_001018012.1.
UniGeneiGga.1399.

Genome annotation databases

GeneIDi552895.
KEGGigga:552895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02827 mRNA. Translation: CAA26595.1.
X03517, X03627 Genomic DNA. Translation: CAA27223.1.
PIRiA23538. SYCHAL.
RefSeqiNP_001018012.1. NM_001018012.1.
UniGeneiGga.1399.

3D structure databases

ProteinModelPortaliP07997.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi552895.
KEGGigga:552895.

Organism-specific databases

CTDi211.

Phylogenomic databases

HOVERGENiHBG005954.
InParanoidiP07997.
KOiK00643.
PhylomeDBiP07997.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.
ReactomeiR-GGA-421525. Heme synthesis.

Miscellaneous databases

PROiP07997.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM1_CHICK
AccessioniPrimary (citable) accession number: P07997
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 1, 1990
Last modified: June 8, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.