Reviewed, UniProtKB/Swiss-Prot P07997 (HEM1_CHICK)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase, nonspecific, mitochondrial
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase
      Short name=ALAS-H

Gene names

Name:	ALAS1
Synonyms:	ALASN

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion matrix.
Miscellaneous	There are two delta-ALA synthetase in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 56	56	Mitochondrion
	Chain	57 – 635	579	5-aminolevulinate synthase, nonspecific, mitochondrial		PRO_0000001233
Amino acid modifications
	Modified residue	440	1	N6-(pyridoxal phosphate)lysine Probable
Experimental info
	Sequence conflict	53	1	S → A Ref.2
	Sequence conflict	53	1	S → A Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P07997-1 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: EEE708B85C1539BA	FASTA	635	69,948
	10 20 30 40 50 60 MEAVVRRCPF LARVSQAFLQ KAGPSLLFYA QHCPKMMEAA PPAAARGLAT SASRGQQVEE 70 80 90 100 110 120 TPAAQPEAKK AKEVAQQNTD GSQPPAGHPP AAAVQSSATK CPFLAAQMNH KSSNVFCKAS 130 140 150 160 170 180 LELQEDVKEM QVDRKGKEFA KIPTNSVVRN TEAEGEEQSG LLKKFKDIML KQRPESVSHL 190 200 210 220 230 240 LQDNLPKSVS TFQYDQFFEK KIDEKKKDHT YRVFKTVNRK AQIFPMADDY SDSLITKKEV 250 260 270 280 290 300 SVWCSNDYLG MSRHPRVCGA VMDTLKQHGA GAGGTRNISG TSKFHVDLEK ELADLHGKDA 310 320 330 340 350 360 ALLFSSCFVA NDSTLFTLAK MLPGCEIYSD SGNHASMIQG IRNSRVPKHI FRHNDVNHLR 370 380 390 400 410 420 ELLKKSDPST PKIVAFETVH SMDGAVCPLE ELCDVAHEHG AITFVDEVHA VGLYGARGGG 430 440 450 460 470 480 IGDRDGVMHK MDIISGTLGK AFACVGGYIS STSALIDTVR SYAAGFIFTT SLPPMLLAGA 490 500 510 520 530 540 LESVRTLKSA EGQVLRRQHQ RNVKLMRQML MDAGLPVVHC PSHIIPIRVA DAAKNTEICD 550 560 570 580 590 600 KLMSQHSIYV QAINYPTVPR GEELLRIAPT PHHTPQMMSY FLEKLLATWK DVGLELKPHS 610 620 630 SAECNFCRRP LHFEVMSERE RSYFSGMSKL LSVSA

References

[1]	"Nucleotide sequence of the chicken 5-aminolevulinate synthase gene." Maguire D.J., Day A.R., Borthwick I.A., Srivastava G., Wigley P.L., May B.K., Elliott W.H. Nucleic Acids Res. 14:1379-1391(1986) [PubMed: 3005973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	"Complete nucleotide sequence of hepatic 5-aminolaevulinate synthase precursor." Borthwick I.A., Srivastava G., Day A.R., Pirola B.A., Snoswell M.A., May B.K., Elliott W.H. Eur. J. Biochem. 150:481-484(1985) [PubMed: 3839458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Expression of delta-aminolevulinate synthase in avian cells: separate genes encode erythroid-specific and nonspecific isozymes." Riddle R.D., Yamamoto M., Engel J.D. Proc. Natl. Acad. Sci. U.S.A. 86:792-796(1989) [PubMed: 2915978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02827 mRNA. Translation: CAA26595.1. X03517, X03627 Genomic DNA. Translation: CAA27223.1.
IPI	IPI00584086.
PIR	SYCHAL. A23538.
RefSeq	NP_001018012.1.
UniGene	Gga.1399
3D structure databases
HSSP	HSSP built from PDB template 1BS0 based on UniProtKB P12998.
ModBase	Search...
Genome annotation databases
GeneID	552895.
KEGG	gga:552895.
Phylogenomic databases
HOVERGEN	P07997.
Enzyme and pathway databases
BRENDA	2.3.1.37. 4.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR004839. Aminotrans_I/II. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_CHICK

Accession

Primary (citable) accession number: P07997

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)