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P07997 (HEM1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-aminolevulinate synthase, nonspecific, mitochondrial

Short name=ALAS-H
EC=2.3.1.37
Alternative name(s):
5-aminolevulinic acid synthase 1
Delta-ALA synthase 1
Delta-aminolevulinate synthase 1
Gene names
Name:ALAS1
Synonyms:ALASN
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactor

Pyridoxal phosphate.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion matrix.

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5656Mitochondrion
Chain57 – 6355795-aminolevulinate synthase, nonspecific, mitochondrial
PRO_0000001233

Sites

Active site4401 By similarity
Binding site2121Substrate By similarity
Binding site3291Substrate By similarity
Binding site3481Substrate By similarity
Binding site3811Pyridoxal phosphate By similarity
Binding site4091Pyridoxal phosphate By similarity
Binding site4371Pyridoxal phosphate By similarity
Binding site4691Pyridoxal phosphate By similarity
Binding site4701Pyridoxal phosphate By similarity
Binding site5571Substrate By similarity

Amino acid modifications

Modified residue4401N6-(pyridoxal phosphate)lysine Probable

Experimental info

Sequence conflict531S → A in CAA26595. Ref.1
Sequence conflict531S → A no nucleotide entry Ref.3

Sequences

Sequence LengthMass (Da)Tools
P07997 [UniParc].

Last modified January 1, 1990. Version 2.
Checksum: EEE708B85C1539BA

FASTA63569,948
        10         20         30         40         50         60 
MEAVVRRCPF LARVSQAFLQ KAGPSLLFYA QHCPKMMEAA PPAAARGLAT SASRGQQVEE 

        70         80         90        100        110        120 
TPAAQPEAKK AKEVAQQNTD GSQPPAGHPP AAAVQSSATK CPFLAAQMNH KSSNVFCKAS 

       130        140        150        160        170        180 
LELQEDVKEM QVDRKGKEFA KIPTNSVVRN TEAEGEEQSG LLKKFKDIML KQRPESVSHL 

       190        200        210        220        230        240 
LQDNLPKSVS TFQYDQFFEK KIDEKKKDHT YRVFKTVNRK AQIFPMADDY SDSLITKKEV 

       250        260        270        280        290        300 
SVWCSNDYLG MSRHPRVCGA VMDTLKQHGA GAGGTRNISG TSKFHVDLEK ELADLHGKDA 

       310        320        330        340        350        360 
ALLFSSCFVA NDSTLFTLAK MLPGCEIYSD SGNHASMIQG IRNSRVPKHI FRHNDVNHLR 

       370        380        390        400        410        420 
ELLKKSDPST PKIVAFETVH SMDGAVCPLE ELCDVAHEHG AITFVDEVHA VGLYGARGGG 

       430        440        450        460        470        480 
IGDRDGVMHK MDIISGTLGK AFACVGGYIS STSALIDTVR SYAAGFIFTT SLPPMLLAGA 

       490        500        510        520        530        540 
LESVRTLKSA EGQVLRRQHQ RNVKLMRQML MDAGLPVVHC PSHIIPIRVA DAAKNTEICD 

       550        560        570        580        590        600 
KLMSQHSIYV QAINYPTVPR GEELLRIAPT PHHTPQMMSY FLEKLLATWK DVGLELKPHS 

       610        620        630 
SAECNFCRRP LHFEVMSERE RSYFSGMSKL LSVSA 

« Hide

References

[1]"Complete nucleotide sequence of hepatic 5-aminolaevulinate synthase precursor."
Borthwick I.A., Srivastava G., Day A.R., Pirola B.A., Snoswell M.A., May B.K., Elliott W.H.
Eur. J. Biochem. 150:481-484(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Nucleotide sequence of the chicken 5-aminolevulinate synthase gene."
Maguire D.J., Day A.R., Borthwick I.A., Srivastava G., Wigley P.L., May B.K., Elliott W.H.
Nucleic Acids Res. 14:1379-1391(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[3]"Expression of delta-aminolevulinate synthase in avian cells: separate genes encode erythroid-specific and nonspecific isozymes."
Riddle R.D., Yamamoto M., Engel J.D.
Proc. Natl. Acad. Sci. U.S.A. 86:792-796(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02827 mRNA. Translation: CAA26595.1.
X03517, X03627 Genomic DNA. Translation: CAA27223.1.
PIRSYCHAL. A23538.
RefSeqNP_001018012.1. NM_001018012.1.
UniGeneGga.1399.

3D structure databases

ProteinModelPortalP07997.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000006285.

Proteomic databases

PaxDbP07997.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID552895.
KEGGgga:552895.

Organism-specific databases

CTD211.

Phylogenomic databases

eggNOGCOG0156.
HOVERGENHBG005954.
KOK00643.
PhylomeDBP07997.

Enzyme and pathway databases

ReactomeREACT_115655. Metabolism.
UniPathwayUPA00251; UER00375.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR01821. 5aminolev_synth. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20879866.
PROP07997.

Entry information

Entry nameHEM1_CHICK
AccessionPrimary (citable) accession number: P07997
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways